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| 1 | Red pigment production by Penicillium purpurogenum GH2 is influenced by pH and temperature显示文摘The combined effects of pH and temperature on red pigment production and fungal morphology were evaluated in a submerged culture of Penicillium purpurogenum GH2, using Czapek-Dox media with D-xylose as a carbon source. An experimental design with a factorial fix was used: three pH values (5, 7, and 9) and two temperature levels (24 and 34 °C) were evaluated. The highest production of red pigment (2.46 g/L) was reached with a pH value of 5 and a temperature of 24 °C. Biomass and red pigment production were not directly associated. This study demon- strates that P. purpurogenum GH2 produces a pigment of potential interest to the food industry. It also shows the feasibility of producing and obtaining natural water-soluble pigments for potential use in food industries. A strong combined effect (p<0.05) of pH and temperature was associated with maximal red pigment production (2.46 g/L). | Alejandro MéNDEZ Catalina PéREZ Julio Cesar MONTAéZ Gabriela MARTíNEZ Cristóbal Noé AGUILAR | 2011 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2011，12，12： | 4 |
| 2 | Aminoguanidine impedes human pancreatic tumor growth and metastasis development in nude mice显示文摘AIM:To study the action of aminoguanidine on pancreatic cancer xenografts in relation to cell proliferation,apoptosis,redox status and vascularization.METHODS:Xenografts of PANC-1 cells were developed in nude mice. The animals were separated into two groups:control and aminoguanidine treated. Tumor growth,survival and appearance of metastases were determined in vivo in both groups. Tumors were excised and ex vivo histochemical studies were performed. Cell growth was assessed by Ki-67 expression. Apoptosis was studied by intratumoral expression of B cell lymphoma-2 protein (Bcl-2) family proteins and Terminal deoxynucleotidyl transferase biotin-dUTP Nick End Labeling (Tunel). Redox status was evaluated by the expression of endothelial nitric oxide synthase (eNOS),catalase,copper-zinc superoxide dismutase (CuZnSOD),manganese superoxide dismutase (MnSOD) and glutathione peroxidase (GPx). Finally,vascularization was determined by Massons trichromic staining,and by VEGF and CD34 expression.RESULTS:Tumor volumes after 32 d of treatment by aminoguanidine (AG) were significantly lower than in control mice (P < 0.01). Median survival of AG mice was significantly greater than control animals (P < 0.01). The appearance of both homolateral and contralateral palpable metastases was significantly delayed in AG group. Apoptotic cells,intratumoral vascularization (trichromic stain) and the expression of Ki-67,Bax,eNOS,CD34,VEGF,catalase,CuZnSOD and MnSOD were diminished in AG treated mice (P < 0.01),while the expression of Bcl-2 and GPx did not change.CONCLUSION:The antitumoral action of aminoguanidine is associated with decreased cell proliferation,reduced angiogenesis,and reduced expression of antioxidant enzymes. | Nora A Mohamad Graciela P Cricco Lorena A Sambuco Máximo Croci Vanina A Medina Alicia S Gutiérrez Rosa M Bergoc Elena S Rivera Gabriela A Martín | 2009 | World Journal of Gastroenterology2009，15，9： | 3 |
| 3 | 更新临床实践指南需要报告的条目:更新版指南的报告清单显示文摘目的临床指南需要根据相关研究结果定期审查以确保其可靠性,但在更新临床指南的过程中仍然缺乏对于更新过程表述的优先报告条目。本文旨在介绍更新版指南报告清单(Checklist for the Reporting of Updated Guidelines,Check Up)及其制定过程。方法基于临床指南更新的研究证据的概述,指南研究与评价工具(Appraisal of Guidelines for Research and EvaluationⅡ,AGREEⅡ)和报告清单专家组(33位)的建议开发了初始清单的各条目。然后,使用多个步骤对本清单进行了完善,包括评估10个现有的更新版临床指南,对关键信息提供者进行访谈[应答率为54.2%(13/24)],清单制定专家组内部进行的一个三轮的德尔菲(Delphi)共识调查,以及来自于临床指南方法学专家[应答率为90%(53/59)]和指南使用者[应答率为55.6%(10/18)]的外部评审意见。结果清单最终包括16个条目,主要涉及:(1)更新版指南的呈现;(2)编辑独立性;(3)更新过程采用的方法。结论更新版指南的报告清单作为一个工具可用于评估更新版指南报告的完整性,并为指南制定者提供撰写报告的要求。 | 王丽琼 严毓倩 梁宁 李迅 费宇彤 刘建平 Vernooij RW Alonso-Coello P Brouwers M Martínez García L | 2017 | 中国中西医结合杂志2017，37，5： | 3 |
| 4 | Faecalibacterium prausnitzii and human intestinal health显示文摘 | S Miquel R Martín O Rossi LG Bermúdez-Humarán JM Chatel H Sokol M Thomas JM Wells P Langella | 2013 | Current Opinion in Microbiology2013，，： | 3 |
| 5 | Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection显示文摘Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components.Dendritic cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease severity.In this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory markers.Remarkably,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 infection.Moreover,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were observed.These findings contribute to a better understanding of the immunological sequelae of COVID-19. | Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-Domínguez Juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-González Luis Fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez JoséManuel Lomas Claudio Bueno Rosario Amaya JoséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo Juan Delgado Antonio Domínguez-Petit Alejandro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-Delgado Manuel García-Gutiérrez María Luisa Gascón-Castillo Aurora González-Estrada Demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis Jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia Lanseros-Tenllado Carmina López Isabel López Álvaro López-Barrios Luis F.López-Cortés Rafael Luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez Luis Martín-Villén JoséMolina Aurora Morillo María Dolores Navarro-Amuedo Dolores Nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez Esther Pérez Manuel Poyato Julia Praena-Segovia Rafaela Ríos Cristina Roca-Oporto Jesús F.Rodríguez María Jesús Rodríguez-Hernández Santiago Rodríguez-Suárez Ángel Rodríguez-Villodres Nieves Romero-Rodríguez Ricardo Ruiz Zida Ruiz de Azua Celia Salamanca Sonia Sánchez Víctor Manuel Sánchez-Montagut César Sotomayor Alejandro Suárez Benjumea Javier Toral | 2021 | Cellular & Molecular Immunology2021，18，9： | 2 |
| 6 | Adipokines,cytokines and body fat stores in hepatitis Cvirus liver steatosis显示文摘AIM: To identify patients with or without liver steatosis and its severity in treatment-na?ve patients affected by hepatitis C virus(HCV) infection.METHODS: We included 56 HCV infected patients, and assessed the amount of liver fat by histomorphometry, and its relationships with fat and lean mass at different parts of the body(by densitometry), hormones [insulin, homeostatic model assessment(HOMA)], adipokines(resistin, adiponectin, leptin), and cytokines(tumor necrosis factor α, interleukin-6).RESULTS: Although the intensity of liver steatosis is related to trunk fat mass and HOMA, 33% of patients showed no liver steatosis, and this finding was not related to body mass index or genotype. Besides trunkfat mass, no other factor was related to the presence or not of liver steatosis, or to the intensity of it, by multivariate analysis. Lean mass was not related to liver steatosis. Adiponectin levels were lower among patients. No differences were observed in leptin and resistin.CONCLUSION: Steatosis in HCV infection is common(67.2%), and closely related to trunk fat, and insulin resistance, but not with leg fat mass or adipokines. | emilio gonzález-reimers javier lópez-prieto geraldine quintero-platt ricardo pelazas-gonzález m remedios alemán-valls onán pérez-hernández m joséde-la-vega-prieto m angeles gómez-rodríguez candelaria martín-gonzález francisco santolaria-fernández | 2016 | World Journal of Hepatology2016，8，1： | 2 |
| 7 | Clinical significance of'anti-HBc alone'in human immunodeficiency virus-positive patients显示文摘AIM:To determine the prevalence and clinical relevance of isolated antibodies to hepatitis B core antigen as the only marker of infection('anti-HBc alone')among human immunodeficiency virus(HIV) type-1 infected patients.Occult hepatitis B infection frequency was also evaluated. METHODS:Three hundred and forty eight histories from 2388 HIV-positive patients were randomly reviewed.Patients with serological markers of hepatitis B virus(HBV)infection were classified into three groups:past hepatitis,'anti-HBc alone'and chronic hepatitis.Determination of DNA from HBV,and RNA and genotype from hepatitis C virus(HCV)were performed on'anti-HBc alone'patients. RESULTS:One hundred and eighty seven(53.7%) HIV-positive patients had markers of HBV infection: 118 past infection(63.1%),14 chronic hepatitis (7.5%)and 55'anti-HBc alone'(29.4%).Younger age[2.3-fold higher per every 10 years younger;95% confidence intervals(CI)1.33-4.00]and antibodies to HCV infection[odds ratio(OR)2.87;95%CI 1.10-7.48]were factors independently associated with the'anti-HBc alone'pattern.No differences in liver disease frequency were detected between both groups. Serum levels of anti-HBs were not associated with HCV infection(nor viral replication or HCV genotype),or with HIV replication or CD4 level.No'anti-HBc alone' patient tested positive for HBV DNA. CONCLUSION:'Anti-HBc alone'prevalence in HIVpositive patients was similar to previously reported data and was associated with a younger age and with antibodies to HCV infection.In clinical practice,HBV DNA determination should be performed only in those patients with clinical or analytical signs of liver injury. | M~aTeresa Pérez-Rodríguez Bernardo Sopea Manuel Crespo Alberto Rivera Teresa González del Blanco Antonio Ocampo César Martínez-Vázquez | 2009 | World Journal of Gastroenterology2009，15，10： | 2 |
| 8 | Usefulness of biodegradable polydioxanone stents in the treatment of postsurgical colorectal strictures and fistulas显示文摘 | F. Pérez Roldán P. González Carro M. Villafá?ez García S. Aoufi Rabih M. Legaz Huidobro N. Sánchez-Manjavacas Mú?oz O. Roncero García-Escribano M. Ynfante Ferrús E. Bernardos Martín F. Ruiz Carrillo | 2012 | Endoscopy2012，，03： | 2 |
| 9 | Collateral avoids ponzi schemes in incomplete markets显示文摘 | ARAUJO A P(A)SCOA M R TORRES MART(I)NEZ J P | | 0，，4： | 1 |
| 10 | Summary of the consensus document:“Clinical practice guide forthe management of low cardiac output syndrome in the postoperativeperiod of heart surgery”显示文摘 | Pérez Vela JL Martín Benitez JC Carrasco Gonzalez M | 2012 | Medicina Intensiva (EnglishEdition)2012，36，4： | 1 |
| 11 | Inflammation, complement, ischemia and amoebic survival in acute experimental amoebic liver abscesses in hamsters显示文摘 | A Olivos-Garc??a M Nequiz-Avenda?o E Tello R.D Mart??nez A González-Canto R López-Vancell M.C Garc??a de León I Montfort R Pérez-Tamayo | 2004 | Experimental and Molecular Pathology2004，，1： | 1 |
| 12 | 显示文摘 | P'EREZ-RUIZ T MART'INEZ-LOZANO C GARC'IA M D | 2007 | J Chromatogr: A2007，1164，12： | 1 |
| 13 | Relationship between crowns and the periodontium:a literature update显示文摘 | Kosyfaki P del Pilar Pinilla Martín M Strub JR | | 0，，02： | 1 |
| 14 | Endocarditis due to Corynebacterium pseudodiphtheriticum显示文摘 | Rivero M Quereda C Martín-Dávila P | 2003 | Enferm Infecc Microbiol Clin2003，21，2： | 1 |
| 15 | Treatment with bisphosphonates in severe hypercalcemia due to subcutaneous fat necrosis in aninfant with hypoxic-ischemic encephalopathy显示文摘 | Pérez Martínez E CamprubíCamprubíM Ramos Cebrián M | 2014 | J Perinatol2014，34，6： | 1 |
| 16 | Experience of off-label use of eltrombopag in the treatment of thrombocytopenia associated with solid tumors显示文摘 | García Lagunar MH Cerezuela Fuentes P Martínez Penella M | 2015 | Farm Hosp2015，39，3： | 1 |
| 17 | Short-term N availability in response to dissolved-organic-carbon from poultry manure, alone or in combination with cellulose显示文摘 | Martín-Olmedo P Rees R M | 1999 | Biology and Fertility of Soils1999，29，： | 1 |
| 18 | Defective hepatic mitochondrial respiratory chain in patients with nonalcoholic steatohepatitis显示文摘 | Pérez-Carreras M Del Hoyo P MartíM A | 2003 | Hepatology2003，38，4： | 1 |
| 19 | Coupled Thermo-Mechanical Analysis of Metal Forming Processes through a Combined Finite Element-Boundary Element Approach显示文摘 | Martings P A F | 1998 | Int J Numer Meth Engng1998，42，： | 1 |
| 20 | Development of a new technology for the production of microcapsules based in atomization processes显示文摘 | Herrero E P Mart'n Del Valle E M Gal'an M A | 2006 | Chem Eng J2006，117，2： | 1 |
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