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| 1 | 土贝母提取物对人乳腺癌MDA-MB-231-GFP裸鼠模型的抗肿瘤作用评价显示文摘目的:建立绿色荧光蛋白标记的人乳腺癌MDA-MB-231-GFP裸鼠模型,研究中药土贝母提取物对转染GFP基因的人乳腺癌MDA-MB-231-GFP裸鼠模型的抗肿瘤活性。方法:应用原位移植方法,建立绿色荧光蛋白标记的人乳腺癌MDA-MB-231-GFP模型。通过比较阴性对照组(口服0.9%氯化钠溶液)、阳性对照组(腹腔注射紫衫醇20mg/kg)、低剂量组(口服土贝母提取物10mg/kg)和高剂量组(口服土贝母提取物50mg/kg)的肿瘤抑制率,评价土贝母提取物对肿瘤生长的影响。结果:阴性对照组与各治疗组小鼠给药前后体质量变化比较,差异均无统计学意义,一般状况良好。低剂量组和阴性对照组间比较,肿瘤大小和体质量的改变差异无统计学意义;高剂量组和阳性对照组肿瘤较阴性对照组明显缩小(P<0.05,P<0.01)。结论:经口服投予较高剂量的土贝母提取物,对人乳癌MDA-MB-231-GFP裸鼠模型有明显的抑制作用。 | 安超 杨萌 胡明昕 傅延龄 李泉旺 曹阳 Robert M.Hoffman 齐慧 刘永刚 胡凯文 | 2013 | 中华中医药杂志2013，28，2： | 12 |
| 2 | Inhibition of growth and metastasis of triple-negative breast cancer targeted by Traditional Chinese Medicine Tubeimu in orthotopic mice models显示文摘Objective: Triple-negative breast cancer(TNBC) is highly invasive and metastatic, which is in urgent need of transformative therapeutics. Tubeimu(TBM), the rhizome of Bolbostemma paniculatum(Maxim.) Franquet, is one of the Chinese medicinal herbs used for breast diseases since the ancient times. The present study evaluated the efficacy, especially the anti-metastatic effects of the dichloromethane extract of Tubeimu(ETBM) on TNBC orthotopic mouse models and cell lines.Methods: We applied real-time imaging on florescent orthotopic TNBC mice model and tested cell migration and invasion abilities with MDA-MB-231 cell line. Digital gene expression sequencing was performed and Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis applied to explore the pathways influenced by ETBM.Moreover, quantitative real-time polymerase chain reactions(q RT-PCR) and Western blot were delivered to confirm the gene expression changes.Results: ETBM exhibited noticeable control on tumor metastasis and growth of TNBC tumors with no obvious toxicity. In compliance with this, it also showed inhibition of cell migration and invasion in vitro. Its impact on the changed biological behavior in TNBC may be a result of decreased expression of integrin β1(ITGβ1), integrin β8(ITGβ8) and Rho GTPase activating protein 5(ARHGAP5), which disabled the focal adhesion pathway and caused change in cell morphology.Conclusions: This study reveals that ETBM has anti-metastatic effects on MDA-MB-231-GFP tumor and may lead to a new therapeutic agent for the integrative treatment of highly invasive TNBC. | Jingxiao Wang Xinjie Yang Haibo Han Limin Wang Weiqian Bao Shanshan Wang Robert M.Hoffman Meng Yang Hui Qi Chao An Kaiwen Hu | 2018 | Chinese Journal of Cancer Research2018，30，1： | 8 |
| 3 | 中药土贝母诱导人乳腺癌细胞凋亡的实时成像显示文摘目的:采用先进的荧光实时显像技术对中药土贝母植物块茎提取物抗乳腺癌作用进行评价。方法:MDA-MB-231人乳腺癌细胞被设计成为细胞质红色荧光蛋白(RFP)基因表达而细胞核组蛋白H2B绿色荧光蛋白(GFP)基因表达的双色荧光细胞,并分别在2D平面塑料平皿及3D立体明胶海绵块两种不同的培养环境中培养,经土贝母鲜品二氯甲烷提取物(DEFT)干预后,荧光共聚焦显微镜下观察细胞形态及核碎片形成的情况;DNA电泳方法进一步检测细胞凋亡。结果:土贝母鲜品二氯甲烷提取物作用24h后荧光显微镜下即观察到MDA-MB-231细胞出现凋亡的形态学改变;DNA电泳在药物干预48h后检测到凋亡的DNA碎片梯带,72h后更加明显;3D明胶海绵培养的MDA-MB-231细胞经实验药物作用后其形态学改变的效果不如2D环境下明显。结论:土贝母鲜品二氯甲烷提取成分具有抗乳腺癌治疗的潜能。 | 胡明昕 安超 李泉旺 Robert M.Hoffman 赵明 杨萌 傅延龄 倪胜楼 胡凯文 | 2012 | 中华中医药杂志2012，27，7： | 5 |
| 4 | 鲜土贝母二氯甲烷提取物对人乳腺癌MDA-MB-231-GFP裸鼠肿瘤生长及肺转移的抑制作用显示文摘目的:研究土贝母鲜品二氯甲烷提取物(DEFT)对绿色荧光蛋白标记的人三阴性乳腺癌MDA-MB-231-GFP裸鼠模型中移植瘤生长和肺转移的作用。方法:应用外科原位移植方法,建立人乳腺癌裸鼠移植模型,随机分成3组,阴性对照组(0.9%Na Cl)、低剂量组(DEFT 50 mg/kg)和高剂量组(DEFT 75 mg/kg),每组5只,灌胃给药,1次/d,连续58 d。每周2次荧光成像系统釆集肿瘤图像,系统自带软件计算肿瘤面积;给药结束时,称取肿瘤质量,荧光成像系统观察肺转移情况。结果:给药前后3组裸鼠体质量变化差异均无统计学意义(P>0.05)。给药第14天,3组肿瘤面积比较,差异有统计学意义(P<0.05)。实验结束时,土贝母低剂量组和高剂量组肺转移的发生均低于阴性对照组(P<0.05)。土贝母低剂量组和高剂量的肿瘤质量低于阴性对照组,但差异无统计学意义(P>0.05)。结论:鲜土贝母二氯甲烷提取物对人三阴性乳腺癌MDA-MB-231-GFP裸鼠模型中肿瘤生长及肺转移的发生有一定抑制作用。 | 鲍伟倩 王婧筱 安超 胡凯文 杨萌 Robert M.Hoffman 齐慧 曹阳 | 2017 | 中医药导报2017，23，23： | 5 |
| 5 | 前列腺癌筛查:调和初级保健和专科的视角矛盾显示文摘当初级保健医生实施专科医生制定的筛查指南时,两者可能因视角不同而产生分歧。两则由专科机构最近发布的临床指南就足以说明这一问题。美国泌尿学会(AUA)指南小组和美国国家综合癌症网络(NCCN)建议,具有前列腺癌一般患病风险的男性在40岁对前列腺特异性抗原筛查风险和好处进行咨询,而此前建议的年龄是50岁。不过,目前没有直接证据证明此建议对前列腺癌死亡率有具体影响。为了不妨碍初级保健医生为患者提供好处和价值已被证实的服务,专业机构在制定指南时应遵循适当的标准。初级保健协会和医疗系统也应该对现有证据进行评估,以判断临床建议实施的可行性和适用性。 | Richard M.Hoffman Michael J.Barry Richard G.Roberts Harold C.Sox 邵钧(翻译) | 2013 | 中国全科医学2013，16，13： | 3 |
| 6 | 维生素A在胚胎形态分化中的作用显示文摘在过去3年里,类视色素(retinoid)特别是视黄酸(retinoic acid)成为发育生物学最热门的课题之一。在一些对胚胎分化起关键作用的组织中检测到了视黄酸活性,并鉴定出了一组特异性的视黄酸受体。从而,视黄酸被确定为胚胎基本的形态原(morphogen)之一。 | M.Hoffman 周虚 | 1991 | 国外畜牧科技1991，18，4： | 2 |
| 7 | Patient-derived orthotopic xenograft models for cancer of unknown primary precisely distinguish chemotherapy,and tumor-targeting S.typhimurium A1-R is superior to first-line chemotherapy显示文摘Cancer of unknown primary(CUP)is a recalcitrant disease with poor prognosis because it lacks standard first-line therapy.CUP consists of diverse malignancy groups,making personalized precision therapy essential.The present study aimed to identify an effective therapy for a CUP patient using a patient-derived orthotopic xenograft(PDOX)model.This paper reports the usefulness of the PDOX model to precisely identify effective and ineffective chemotherapy and to compare the efficacy of S.typhimurium A1-R with first-line chemotherapy using the CUP PDOX model.The present study is the first to use a CUP PDOX model,which was able to precisely distinguish the chemotherapeutic course.We found that a carboplatinum(CAR)-based regimen was effective for this CUP patient.We also demonstrated that S.typhimurium A1-R was more effective against the CUP tumor than first-line chemotherapy.Our results indicate that S.typhimurium A1-R has clinical potential for CUP,a resistant disease that requires effective therapy. | Kentaro Miyake Tasuku Kiyuna Masuyo Miyake Kei Kawaguchi Sang Nam Yoon Zhiying Zhang Kentaro Igarashi Sahar Razmjooei Sintawat Wangsiricharoen Takashi Murakami Yunfeng Li Scott D.Nelson Tara A.Russell Arun S.Singh Yukihiko Hiroshima Masashi Momiyama Ryusei Matsuyama Takashi Chishima Shree Ram Singh Itaru Endo Fritz C.Eilber Robert M.Hoffman | 2018 | Signal Transduction and Targeted Therapy2018，3，1： | 2 |
| 8 | Fluorescence-guided hepatobiliary surgery with long and short wavelength fluorophores显示文摘Importance:Fluorescence-guided surgery(FGS)is a potentially powerful tool for hepatobiliary(HPB)surgery.The high sensitivity of fluorescence navigation is especially useful in settings where tactile feedback is limited.Objective:The present narrative review evaluates literature on the use of FDA-approved fluorophores such as methylene blue(MB),5-aminolevulinic acid(5-ALA),and indocyanine green(ICG)for clinical intra-operative image-guidance during HPB surgery.Evidence Review:Approaches such as dosing,timing,imaging devices and comparative endpoints are summarized.The feasibility and safety of fluorophores in visualizing the biliary tree,identify biliary leaks,outline anatomic hepatic segments,identify tumors,and evaluate perfusion and graft function in liver transplants are discussed.Findings:Tumor-specific probes are a promising advancement in FGS with a greater degree of specificity.The current status of tumor-specific probes being evaluated in clinical trials are summarized.Conclusions and Relevance for Reviews:Relevant discussion of promising tumor-specific probes in pre-clinical development are discussed.Fluorescence-guidance in HPB surgery is relatively new,but current literature shows that the dyes are reliably able to outline desired structures with a variety of dosing,timing,and imaging devices to provide real-time intra-operative anatomic information to surgeons.Development of tumor-specific probes will further advance the field of HPB surgery especially during oncologic resections. | Thinzar M.Lwin Robert M.Hoffman Michael Bouvet | 2020 | Hepatobiliary Surgery and Nutrition2020，9，5： | 2 |
| 9 | IL-1-3 IL-1 and the inflammasome显示文摘 | Hal M.Hoffman | | 0，，： | 1 |
| 10 | Cimetidine: An inhibitor or promoter of tumor growth?显示文摘 | Aaron R.Sasson RezaGamagami ZiliAn XiaoenWang A.R.Moossa Robert M.Hoffman | 1999 | Int J Cancer1999，，5： | 1 |
| 11 | The effective mechanism of counterproductive work behavior based on the exchange relationship in work ploce显示文摘 | David P Mackinnon chondra M.Lockwood Jeanne M.Hoffman Stephen G West Virgil sheets | | 0，，01： | 1 |
| 12 | renleixuejia weihe yao yanjiu zhainan (Why Should Anthropologists Study Disaster)显示文摘 | Anthony Oliver Smith Susanna M.Hoffman Peng Wen-bin | | 0，，04： | 1 |
| 13 | A Conceptualization of Trust in International Relations显示文摘 | Aaronm M.Hoffman | | 0，，03： | 1 |
| 14 | The marketing of diversity in the inner city: tourism and regulation in Harlem显示文摘 | Lily M.Hoffman | 2003 | International Journal of Urban and Regional Research2003，，2： | 1 |
| 15 | Speed associated with plasma pH, oxygen content, total protein and urea in an 80 km race显示文摘 | R. M.HOFFMAN T. M.HESS C. A.WILLIAMS D. S.KRONFELD K. M.GRIEWE‐CRANDELL J. E.WALDRON P. M.GRAHAM‐THIERS L. S.GAY R. K.SPLAN K. E.SAKER P. A.HARRIS | 2010 | Equine Veterinary Journal . 2010 (S34)2010，，34： | 1 |
| 16 | Pharmacotherapy perspectives-pantoprazole sodium for injection显示文摘 | James M.Hoffman Lee Vermeulen | | 0，，01： | 1 |
| 17 | Review article: the prothrombin time test as a measure of bleeding risk and prognosis in liver disease显示文摘 | A.TRIPODI S. H.CALDWELL M.HOFFMAN J. F.TROTTER A. J.SANYAL | 2007 | Alimentary Pharmacology & Therapeutics2007，，2： | 1 |
| 18 | Leaching of diethylhexyl phthalate from polyvinyl chloride bags into intravenous etoposide solution显示文摘 | B.Demore J.Viqneron A.Perrin M.A.Hoffman M.Hoffman | | 0，，02： | 1 |
| 19 | Role of the leucine‐rich repeat domain of cryopyrin/NALP3 in monosodium urate crystal–induced inflammation in mice显示文摘 | Hal M.Hoffman PeterScott James L.Mueller AmirMisaghi SeanStevens George D.Yancopoulos AndrewMurphy David M.Valenzuela RuLiu‐Bryan | 2010 | Arthritis & Rheumatism2010，，7： | 1 |
| 20 | 关于聚焦诱导立体显示器的研究显示文摘传统的立体显示是通过聚焦诱导,进行深度视觉模糊调节,从而达到立体视觉效果。现在,科研人员开发了一种新型技术——聚焦修正。通过这项技术,将聚焦诱导和深度视觉模仿同步进行,可以使立体景象显示更加快速、准确,同时减轻观看者的视觉疲劳度。 | Martin S.Banks Kurt A keley David M.Hoffman Ahna R.Girshick 王鹏 | 2009 | 现代显示2009，，2： | 0 |
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