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| 1 | Down.regulation of E.cadherin enhances prostate cancer chemoresistance via Notch signaling显示文摘Background:The chemoresistance of prostate cancer(PCa)is invariably associated with the aggressiveness and metastasis of this disease.New emerging evidence indicates that the epithelial-to-mesenchymal transition(EMT)may play pivotal roles in the development of chemoresistance and metastasis.As a hallmark of EMT,E-cadherin is suggested to be a key marker in the development of chemoresistance.However,the molecular mechanisms underlying PCa chemoresistance remain unclear.The current study aimed to explore the association between EMT and chemoresistance in PCa as well as whether changing the expression of E-cadherin would affect PCa chemoresistance.Methods:Parental PC3 and DU145 cells and their chemoresistant PC3-Tx R and DU145-Tx R cells were analyzed.PC3-Tx R and DU145-Tx R cells were transfected with E-cadherin-expressing lentivirus to overexpress E-cadherin;PC3 and DU145 cells were transfected with small interfering RNA to silence E-cadherin.Changes of EMT phenotype-related markers and signaling pathways were assessed by Western blotting and quantitative real-time polymerase chain reaction.Tumor cell migration,invasion,and colony formation were then evaluated by wound healing,transwell,and colony formation assays,respectively.The drug sensitivity was evaluated using MTS assay.Results:Chemoresistant PC3-Tx R and DU145-Tx R cells exhibited an invasive and metastatic phenotype that associated with EMT,including the down-regulation of E-cadherin and up-regulation of Vimentin,Snail,and N-cadherin,comparing with that of parental PC3 and DU145 cells.When E-cadherin was overexpressed in PC3-Tx R and DU145-Tx R cells,the expression of Vimentin and Claudin-1 was down-regulated,and tumor cell migration and invasion were inhibited.In particular,the sensitivity to paclitaxel was reactivated in E-cadherin-overexpressing PC3-Tx R and DU145-Tx R cells.When E-cadherin expression was silenced in parental PC3 and DU145 cells,the expression of Vimentin and Snail was up-regulated,and,particularly,the sensitivity to paclitaxel was decreased.Interestingly,Notch-1 expression was up-regulated in PC3-Tx R and DU145-Tx R cells,whereas the E-cadherin expression was down-regulated in these cells comparing with their parental cells.The use ofγ-secretase inhibitor,a Notch signaling pathway inhibitor,significantly increased the sensitivity of chemoresistant cells to paclitaxel.Conclusion:The down-regulation of E-cadherin enhances PCa chemoresistance via Notch signaling,and inhibiting the Notch signaling pathway may reverse PCa chemoresistance. | Wenchu Wang Lihui Wang Atsushi Mizokami Junlin Shi Chunlin Zou Jinlu Dai Evan T. Keller Yi Lu Jian Zhang | 2017 | Chinese Journal of Cancer2017，36，3： | 16 |
| 2 | Effects and safety of aspirin use in patients after cerebrovascular bypass procedures显示文摘Object Superficial temporal artery to middle cerebral artery(STA-MCA)bypass is the most effective treatment for Moyamoya disease(MMD).In this study,we aimed to assess whether aspirin improves STA-MCA bypass patency and is safe in patients with MMD.Methods We performed a retrospective medical record review of patients with ischaemic-onset MMD who had undergone STA-MCA bypass at two hospitals between January 2011 and August 2018,to clarify the effects and safety of aspirin following STA-MCA bypass.The neurological status at the last follow-up(FU)was compared between patients with FU bypass patency and occlusion.Results Among 217 identified patients(238 hemispheres),the mean age was 41.4±10.2 years,and 51.8%were male;the indications for STA-MCA bypass were stroke(48.2%),followed by a transient ischaemic attack(44.0%).Immediate bypass patency was confirmed in all cases.During the FU period(1.5±1.5 y),15 cases were occluded at FU imaging,resulting in an overall cumulative patency rate of 94%.The patency rates were 93%and 94%in the short-term FU group(n=131,mean FU time 0.5±0.2 years)and long-term FU group(n=107,mean FU time 4.1±3.5 years),respectively.The STA-MCA bypass patency rate in the aspirin group was higher than that in the non-aspirin group(98.7%vs 89.7%;HR 1.57;95%CI 1.106 to 2.235;p=0.012).No significant difference in the FU haemorrhagic events was observed between the aspirin and non-aspirin groups.Conclusions Among adult patients with ischaemic-onset MMD undergoing STA-MCA bypass procedures,aspirin might increase the bypass patency rate,without increasing the bleeding risk.FU bypass patency may be associated with a better outcome.Additional studies,especially carefully designed prospective studies,are needed to address the role of aspirin after bypass procedures. | Junlin Lu Guangchao Shi Yuanli Zhao Rong Wang Dong Zhang Xiaolin Chen Hao Wang Ji Zong Zhao | 2021 | Stroke & Vascular Neurology2021，6，4： | 7 |
| 3 | Characteristics of black carbon aerosol in Jiaxing,China during autumn 2013显示文摘We conducted measurements of black carbon(BC) aerosol in Jiaxing,China during autumn from September 26 to November 30,2013.We investigated temporal and diurnal variations of BC,and its correlations with meteorological parameters and other major pollutants.Results showed that hourly mass concentrations of BC ranged from 0.2 to 22.0 μg/m^3,with an average of 5.1 μg/m^3.The diurnai variation of BC exhibited a bimodal distribution,with peaks at 07:00 and 18:00.The morning peak was larger than the evening peak.The mass percentages of BC in PM_(2.5) and PM_(10) were 7.1%and 4.8%,respectively.The absorption coefficient of BC was calculated to be 44.4 Mm^(-1),which accounted for 11.1%of the total aerosol extinction.BC was mainly emitted from local sources in southwestern Jiaxing where BC concentrations were generally greater than 11 μg/m^3 during the measurement period.Correlation analysis indicated that the main sources of BC were motor vehicle exhaust,and domestic and industrial combustion. | Lijuan Shen Li Li Sheng Lu Xiaohan Zhang Jie Liu Junlin An Guojun Zhang Bo Wu Fei Wang | 2015 | Particuology2015，13，3： | 2 |
| 4 | Quantitative angiographic haemodynamic evaluation of bypasses for complex aneurysms: a preliminary study显示文摘Objective Open microsurgery,often with bypass techniques,is indispensable for complex aneurysms.To date,it remains unknown whether arterial anatomy or quantitative blood flow measurements can predict insufficient flow-related stroke(IRS).The present study aimed to evaluate the risk factors for IRS in patients treated with open microsurgery with bypass procedures for complex internal carotid artery aneurysms.Methods Patients with complex aneurysms undergoing bypass surgery were retrospectively reviewed.The recipient/donor flow index(RDFI)was preoperatively evaluated using colour-coding angiography.RDFI was defined as the ratio of the cerebral blood volume of the recipient and donor arteries.The sizes of the recipient and donor arteries were measured.The recipient/donor diameter index(RDDI)was then calculated.IRS was defined as the presence of new postoperative neurological deficits and infarction on postoperative CT scans.We assessed the association between RDFI and other variables and the IRS.Results Twenty patients(38±12 years)were analysed.IRS was observed in 12 patients(60%).Patients with postoperative IRS had a higher RDFI than those without postoperative IRS(p<0.001).RDDI was not significantly different between patients with and without IRS(p=0.905).Patients with RDFI>2.3 were more likely to develop IRS(p<0.001).Conclusion Quantitative digital subtraction angiography enables preoperative evaluation of cerebral blood volume.RDFI>2.3,rather than RDDI,was significantly associated with postoperative IRS.This preoperative evaluation allows appropriate decisions regarding the treatment strategy for preventing postoperative IRS. | Junlin Lu Chao Xue Xulin Hu Yuanli Zhao Dong Zhang Xiaolin Chen Ji Zong Zhao | 2022 | Stroke & Vascular Neurology2022，7，1： | 1 |
| 5 | FPGA Prototyping of an AMBA Based Windows-Compatible SoC显示文摘 | Kan Huang Junlin Lu Jiufeng Pang | 2010 | FPGA''10 Feb 21-232010，，： | 1 |
| 6 | E-cadherin表达下调激活Notch信号通路增强前列腺癌化疗耐药的机制研究显示文摘背景与目的前列腺癌(prostate cancer,PCa)化疗耐药与其侵袭性和转移性密切相关。最新研究显示上皮细胞–间充质转化(epithelial-to-mesenchymal transition,EMT)可能在前列腺癌化疗耐药和转移中发挥重要作用。因此,作为EMT的标志物,E-cadherin被认为有可能与肿瘤化疗耐药有重要关系。然而,PCa化疗耐药的分子机制尚不清楚。本研究旨在探讨EMT与PCa化疗耐药的关系,以及E-cadherin表达变化是否影响PCa化疗耐药。方法以亲代PC3和DU145细胞及其化疗耐药PC3-TxR和DU145-TxR细胞作为研究对象。用E-cadherin过表达的慢病毒感染PC3-TxR和DU145-TxR细胞,使E-cadherin过表达;同时,用小干扰RNA转染PC3和DU145细胞,使E-cadherin表达沉默。采用蛋白免疫印迹和实时定量聚合酶链反应分析EMT相关标志物及信号通路的变化。分别通过划痕、transwell和克隆形成实验检测肿瘤细胞的迁移、侵袭和克隆形成能力。采用MTS法检测细胞对紫杉醇的药物敏感性。结果与亲代PC3和DU145细胞相比,化疗耐药PC3-TxR和DU145-TxR细胞表现出EMT相关的标志物表达改变,包括E-cadherin表达下调和Vimentin、Snail与N-cadherin表达上调。当E-cadherin在PC3-TxR和DU-145-TxR细胞中过表达时,Vimentin和Caldun-1表达下调,肿瘤细胞的迁移和侵袭受到抑制。特别是E-cadherin过表达以后,PC3-TxR和DU145-TxR细胞对紫杉醇的敏感性被重新激活。并且,在亲代PC3和DU145细胞中沉默E-cadherin表达后,Vimentin和Snail表达上调,对紫杉醇的敏感性降低。有趣的是,与亲代PC3和DU145细胞相比,PC3-TxR和DU145-TxR细胞中Notch-1表达上调,而E-cadherin在这些细胞中的表达下调。我们的研究还显示,Notch信号通路抑制剂γ-分泌酶抑制剂可显著增加化疗耐药细胞对紫杉醇的敏感性。结论 E-cadherin下调可以激活Notch信号通路,从而增强PCa化疗耐药,抑制Notch信号通路可能逆转PCa化疗耐药。 | Wenchu Wang Lihui Wang Atsushi Mizokami Junlin Shi Chunlin Zou Jinlu Dai Evan T.Keller Yi Lu Jian Zhang | 2019 | 癌症2019，38，3： | 1 |
| 7 | A quality by design (QbD) case study on entericcoated pellets: Screening of critical variables and establishment of design space at laboratory scale显示文摘The study aims to prepare naproxen enteric-coated pellets(NAP-ECPs)by fluid-bed coating using QbD principle.Risk assessment was firstly performed by using failure mode and effect analysis(FMEA)methodology.A PlacketteBurman design was then used for assessment of the most important variables affecting enteric-coated pellets characteristics.A BoxeBehnken design was subsequently used for investigating the main,interactive,and quadratic effects of these variables on the response.By FMEA we discovered that eight factors should be considered to be high/important risk variables as compared with others.The responses of acid resistance and cumulative drug release were taken as critical quality attributes(CQAs).Pareto ranking analyses indicated that the coating weight gain(X_(7)),triethyl citrate percentage(X_(1))and glycerol monostearate percentage(X_(2))were the most significant factors affecting the selected responses out of the eight high-risk variables.Optimization with response surface method(RSM)further fully clarified the relationship between X_(7),X_(1),X_(2) and CQAs,and design space was established based on the constraints set on the responses.Due to the extreme coincidence of the predicted value generated by model with the observed value,the accuracy and robustness of the model were confirmed.It could be concluded that a promising NAP-ECPs was successfully designed using QbD approach in a laboratory scale. | Shuling Kan Jing Lu Jianping Liu Junlin Wang Yi Zhao | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，5： | 1 |
| 8 | Interaction of inter- metallic compound formation in Cu/SnAgCu/NiAu sandwich solder joints显示文摘 | Xia Yanghua Lu Chuanyan Chang Junlin | 2006 | Journal of Electronic Materials2006，35，5： | 1 |
| 9 | Investigation of the feasibility of NRAV as a biomarker for hepatocellular carcinoma显示文摘The long non-coding RNA,Negative Regulator of Antiviral Response(NRAV)has been identified as a participant in both respiratory virus replication and immune checkpoints,however,its involvement in pan-cancer immune regulation and prognosis,particularly those of hepatocellular carcinoma(HCC),remains unclear.To address this knowledge gap,we analyzed expression profiles obtained from The Cancer Genome Atlas(TCGA)database,comparing normal and malignant tumor tissues.We found that NRAV expression is significantly upregulated in tumor tissues compared to adjacent nontumor tissues.Kaplan-Meier(K-M)analysis revealed the prognostic power of NRAV,wherein overexpression was significantly linked to reduced overall survival in a diverse range of tumor patients.Furthermore,noteworthy associations were observed between NRAV,immune checkpoints,immune cell infiltration,genes related to autophagy,epithelial-mesenchymal transition(EMT),pyroptosis,tumor mutational burden(TMB),and microsatellite instability(MSI)across different cancer types,including HCC.Moreover,NRAV upregulation expression was associated with multiple pathological stages by clinical observations.Furthermore,our investigation revealed a substantial elevation in the expression of NRAV in both HCC tumor tissues and cells compared to normal tissues and cells.The inhibition of NRAV resulted in the inhibition of cell proliferation,migration,and invasion in HCC cells,while also influencing the expression of CD274(PD-L1)and CD44,along with various biomarkers associated with EMT,autophagy,and pyroptosis.The aforementioned results propose NRAV as a promising prognostic biomarker for HCC. | JUN LIU WENLI LI RUYUE LU JIAQING XU CHUNHUI JIANG JUNLIN DUAN LINGZHI ZHANG GUANFU WANG JIAXI CHEN | 2024 | Oncology Research2024，32，4： | 0 |
| 10 | A facile strategy of in-situ anchoring of Co_(3)O_(4) on N doped carbon cloth for an ultrahigh electrochemical performance显示文摘Enhancement of supercapacitors(SCs)with high-energy density and high-power density is still a great challenge.In this paper,a facile strategy for in situ anchoring of Co_(3)O_(4) particles on N doped carbon cloth(pCoNCC)is reported.Due to the interaction of the doped N and Co_(3)O_(4),the electrochemical performance improves significantly,reaching 1,940.13 mF·cm^(−2) at 1 mA·cm^(−2) and energy density of 172.46μWh·cm^(−2) at the power density of 400μW·cm^(−2),much larger than that without N doping electrode of 28.5 mF·cm^(−2).An aqueous symmetric supercapacitor(ASSC)assembled by two pCoNCC electrodes achieves a maximum energy density of 447.42μWh·cm^(−2) and a highest power density of 8,000μW·cm^(−2).Utilizing such a high-energy storage ASSC,a digital watch and a temperature-humidity detector are powered for nearly 1 and 2 h,respectively.Moreover,the ASSC displays a superb electrochemical stability of 87.7%retention after 10,000 cycles at 40 mA·cm^(−2).This work would provide a new sight to enhance active materials performance and be beneficial for the future energy storage and supply systems. | Junlin Lu Jien Li Jing Wan Xiangyu Han Peiyuan Ji Shuang Luo Mingxin Gu Dapeng Wei Chenguo Hu | 2021 | Nano Research2021，14，7： | 0 |
| 11 | In vitro expression and analysis of the 826 human G protein-coupled receptors显示文摘G 联合蛋白质的受体(GPCR ) 涉及他们为 transducing 负责的所有人的生理的系统细胞外的信号进房间。GPCR 包括光,荷尔蒙,和类脂化合物响应刺激的一个多样的数组发信号,在这些信号影响下游的串联影响健康和疾病状态的地方。然而,尽管有他们是的重要性治疗学的目标,仅仅 30 GPCR 的详细分子的结构迄今为止被决定了。对他们的结构决心的关键挑战是足够的蛋白质表示。这里,我们用二不同熔化构造为所有 826 人的 GPCR 在一个昆虫房间表达式系统报导蛋白质表示的 quantification。表示特征在总数并且在每五个不同亚科之中被分析。这些数据能被用来识别与在不同熔化构造之间并且在不同 GPCR 家庭之间的 GPCR 表示有关的趋势,并且为未来结构决心可行性优先考虑铅候选人。 | Xuechen Lv Junlin Liu Qiaoyun Shi Qiwen Tan Dong Wu John J. Skinner Angela L. Walker Lixia Zhao Xiangxiang Gu Na Chen Lu Xue Pei Si Lu Zhang Zeshi Wang Vsevolod Katritch Zhijie Liu Raymond C. Stevens | 2016 | Protein & Cell2016，7，5： | 0 |
| 12 | Recent Advancements in Petroleum E & P Equipments in China显示文摘The role of equipments in oil and gas exploration and development had never been attached with so much importance as that in shale oil and gas boom in the U.S.With the help of massive hydraulic fracturing and horizontal drilling techniques,the U.S.,the world’s No.1 oil importer even started to dream about energy selfsufficiency with its proudly high production of shale | Lu Xuemei Lu Junlin Zhang Yuyin Wu Xinming | 2014 | China Oil & Gas2014，21，3： | 0 |
| 13 | Detect Peripheral Hardware Faults Using I/O-state-based Dynamic Value Invariants显示文摘 | ZHENG Yansong LU Junlin TONG Dong WANG Keyi CHENG Xu | 2012 | Chinese Journal of Electronics2012，21，2： | 0 |
| 14 | Effects of degradation and species composition on soil seed density in the alpine grasslands, China显示文摘Grassland degradation can alter the structure and function of ecosystem and soil seed bank.Therefore,estimating the role of soil seed bank in vegetation regeneration of degraded grasslands is crucial.We selected grasslands with three levels of degradation,namely non-degraded(ND),mildly degraded(MD),and heavily degraded(HD)to analyze the effect of grassland degradation on soil seed bank,as well as the role of soil seed bank on vegetation regeneration of the alpine grasslands,China.Soil samples from each level were collected in May,before seedling emergence,in August,after completion of transient seed bank germination,and in December,after seed dispersal,to determine the seed density and species composition through germination experiment.Result showed that a total of 35 plant species was identified,including 15 species observed in both soil seed bank and above-ground vegetation.A total of 19,15,and 14 species of soil seed bank were identified in December,May,and August,respectively.The most abundant species in soil seed bank were Compositae(5 species),followed by Poaceae(4 species),and Cyperaceae(3 species).Degradation level has no significant impact on species richness and Shannon-Wiener index of soil seed bank.In addition,sampling month and grassland degradation affected soil seed bank density,in which December>May>August,and ND>MD>HD,indicating that density of transient seed bank was greater than persistent seed bank.Soil seed bank density of surface layer(0–5 cm)accounting for 42%–72%of the total density,which was significantly higher than that of deep layer(5–10 cm).Similarity of species composition between vegetation and soil seed bank was low,and it increased with degradation level(ranged from 0.14 to 0.69).We concluded that grassland degradation affects soil seed bank density more than species diversity,and soil seed bank contributed slightly to vegetation regeneration of degraded alpine grassland.Therefore,it is unlikely that degraded alpine meadow can be restored solely through soil seed bank. | LI Chunming MA Jiahui LI Liangyu HUANG Junlin LU Jinhua HUANG Mei Allan DEGEN SHANG Zhanhuan | 2023 | Journal of Arid Land2023，15，12： | 0 |
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