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| 1 | 小胶质细胞对星形胶质细胞的免疫和神经保护功能的调节作用(英文)显示文摘在中枢神经系统中,小胶质细胞和星形胶质细胞对神经元的发育、功能维持及细胞存活具有重要的作用。但在炎症反应过程中,星形胶质细胞增生的作用及其对神经元的影响目前并不十分清楚。因此,本研究对不同种类的细胞进行培养,给予细菌脂多糖(lipopolysaccharide,LPS)刺激,观察各种细胞的反应。结果显示,星形胶质细胞可以减轻炎症反应的神经元毒性,提示星形胶质细胞增生主要具有神经保护作用。如果去掉上清液中由星形胶质细胞分泌的胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF),这种神经保护作用显著降低。为了研究星形胶质细胞的免疫功能,本研究培养了高浓度的星形胶质细胞,并发现LPS不能诱导肿瘤坏死因子(tumor necrosis factor,TNF)-α及诱导型一氧化氮合酶(inducible nitric oxide synthase,iN OS)/一氧化氮(nitric oxide,NO)(iN OS/NO)释放。但如果在培养的高浓度星形胶质细胞中增加0.5%~1%的小胶质细胞,则可以诱导TNF-α及iN OS/NO的释放。这提示小胶质细胞和星形胶质细胞间的相互作用对于LPS诱导星形胶质细胞释放前炎症因子及GDNF是必须的。我们还发现,小胶质细胞释放的TNF-α可以通过旁分泌作用调节星形胶质细胞的神经保护功能。综上所述,本研究结果提示星形胶质细胞可能不会直接被LPS激活,而是通过与小胶质细胞相互作用被激活,释放神经营养因子,减少因炎症反应激活的小胶质细胞释放的毒性物质对神经元的损害作用。 | Chen SH Oyarzabal EA Sung YF Chu CH Wang Q Chen SL Lu RB Hong JS 唐颖馨 | 2015 | 神经损伤与功能重建2015，10，1： | 9 |
| 2 | Numerical Modeling on Suspended Sediment Transportation in the Hangzhou Bay显示文摘In this paper the characteristics of tidal flow and seasonal variation of seidment content in theHangzhou Bay and their affecting factors are studied.Field investigations and data analysis indicate thatthe sediment movement is mainly influenced by the Yangtze estuary and the sediment of the Yangtze estua-ry is induced by wind wave and tidal flow.Owing to the variation of dynamic conditions,the instanta-neous sediment content is controlled by tidal flow,wind wave,depth of water and tidal range synthetically.A sediment content relationship formula is established with related factors.A non-equilibrium2-dimensional numerical model of suspended sediment transportation is set up,and the finite element meth-od is applied.The computation results of the model is in accordance with field data. | Zhang, JS Lu, PD Xu, M | 1998 | China Ocean Engineering1998，13，1： | 7 |
| 3 | VIP immunoreactive nerves and somatostatin and serotonin containing cellsin Crohn′s disease显示文摘 | Lu SJ Liu YQ Lin JS Wu HJ Sun YH Tan YB | 1999 | World Journal of Gastroenterology1999，5，6： | 4 |
| 4 | Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles显示文摘 | Zhao P Qin ZL Ke JS Lu Y Liu M Pan W Zhao LJ Cao J Qi ZT | 2005 | 第二军医大学学报2005，26，10： | 4 |
| 5 | Lipopolysaccharide decreasing albumin expression in rat hepatocytes显示文摘The relations of the severity of hypoalbu-minemia to morbidity and mortality of patients with critical illness illustrate the need for better understanding of molecular mechanism of hypoalbuminemia. This study was undertaken to investigate the response of albumin synthesis to lipopolysaccharide(LPS)in rat hepatocytes in vitro in early acute phase of sepsis. METHODS:Hepatocytes were cultured at an initial cell density of 1.5×106 cells/well in 3 ml culture medium. There were two groups of samples which received either normal saline or 1 μg/L LPS randomly. Albumin mRNA in hepatocytes was assessed by reverse transcription-poly-merase chain reaction(RT-PCR) and albumin level in the supernatant was measured by ELISA at 0,2,8,12,24 hours after exposure. Meanwhile, the albumin precursor was evaluated at the same time points by flow cytometry. RESULTS:The quantitative changes of mRNA, albumin precursor and its protein were analogous. All of them tended to decline at 12 hours post-treatment and did not decrease significantly until 24 hours after LPS exposure. Meanwhile, albumin mRNA decreased about 30% and the levels of albumin precursor and albumin reduced approximately 50%. CONCLUSIONS: LPS can inhibit albumin synthesis in rat hepatocytes by prevention of albumin transcription. Moreover, the response of hepatic albumin synthesis to LPS changes with the stage of sepsis process. The results show that albumin metabolism in sepsis is a complicated process and further studies are required to understand the molecular mechanism of LPS-induced hypoalbuminemia in sepsis. | Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, China (Wang XY, Li WQ, Li N and Li JS) and Department of Orthopaedics, Zhongda Hospital, Nanjing 210009, China (Lu J) | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 2 |
| 6 | EPIMEDOKOREANONE A FROM THE AERIAL PARTS O FEPIMEDIUM KOREANUM NAKAI显示文摘EPIMEDOKOREANONEAFROMTHEAERIALPARTSOFEPIMEDIUMKOREANUMNAKAIWenKuiLI;JunShanYANG;PeiGenXIAO(InstituteofMedicinalPlantDevelopme... | Li, WK Yang, JS Xiao, PG Pan, JQ Lu, MJ Zhang, RY | 1996 | Chinese Chemical Letters1996，7，1： | 2 |
| 7 | 1 Effects ofpotassium supplemen-tmion on the recovery of thyrotoxic periodic paralysis显示文摘 | Lu KC Hsu YJ Chiu JS eta | 2004 | Am JEmergMed2004，22，7： | 1 |
| 8 | ER alpha negative breast cancer cells restore response to endocrine therapy by combination treatment with both HDAC inhibitor and DNMT inhibitor显示文摘 | Fan J Yin WJ Lu JS | 2008 | J Cancer Res Clin On- col2008，134，8： | 1 |
| 9 | Combination of active components of Xiexin decoction ameliorates renal fibrosis through the inhibition of NF -kappaB and TGF - betal/Smad pathways in db/db diabetic mice 显示文摘 | Wu JS Shi R Lu X | 2015 | PLoS One2015，10，01： | 1 |
| 10 | ER alpha negative breast cancer cells restore response to endoc-rine therapy by combination treatment with both HDAC inhibitor and DNMT inhibitor显示文摘 | Fan J Yin WJ Lu JS | 2008 | J Cancer Res Clin Oncol2008，134，8： | 1 |
| 11 | Motion Estimation Algorithm Using Interblock Correlation显示文摘 | HSIEH CH LU PC SHYN JS | 1990 | Electronics Letters1990，26，5： | 1 |
| 12 | Correlation between serum lipid concentrations and psychological distress显示文摘 | Chen CC Lu FH Wu JS | | 0，，02： | 1 |
| 13 | Turbulance characteristics downstream ofbileaflet aortic valve prostheses显示文摘 | Liu JS Lu PC Chu SH | 2000 | J Biomech Eng2000，122，2： | 1 |
| 14 | Fiberoptic ductoscopycombined with cytology testing in the patients of sponta-neous nipple discharge显示文摘 | Liu GY Lu JS Shen KW | 2008 | Breast Cancer Res Treat2008，108，2： | 1 |
| 15 | Imaging in the diagnosis and follow-up evaluation of vertebral artery dissection显示文摘 | Lu CJ Sun Y Jeng JS | 2000 | J Ultrasound Med2000，19，： | 1 |
| 16 | A comparative study of pulse rate variability and heart rate variability in healthy subjects显示文摘 | Wong JS Lu WA Wu KT | 2012 | J Clin Monit Comput2012，26，2： | 1 |
| 17 | Role of vortices in cavitation formation in the flow across a mechanical heart valve显示文摘 | Li CP Lu PC Liu JS | | 0，，4： | 1 |
| 18 | Cytokine production at the site of disease in human tuberculoses显示文摘 | Bsrnes PF Lu S Abrams JS | 1993 | Infect Immunol1993，61，： | 1 |
| 19 | Role of calcium and cyclophilin D in the regulation of mitochondrial permeabilization induced by glutathione depletion 显示文摘 | Lu C Armstrong JS | 2009 | Biochem Biophys Res Commun2009，363，3： | 1 |
| 20 | Adenovirus-mediated siRNA targeting c-Met inhibits proliferation and invasion of small-cell lung cancer(SCLC) cells显示文摘 | Wang ZX Lu BB Yang JS | 2011 | J Surg Res2011，171，1： | 1 |
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