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2篇 您的检索式：作者名="Linwei Duan"

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		题名	作者	年代	出处	被引量
	1	Responses of the functional structure of soil microbial community to livestock grazing in the T ibetan alpine grassland显示文摘	Yunfeng Yang Linwei Wu Qiaoyan Lin Mengting Yuan Depeng Xu Hao Yu Yigang Hu Jichuang Duan Xiangzhen Li Zhili He Kai Xue Joy van Nostrand Shiping Wang Jizhong Zhou	2012	Glob Change Biol2012，，2：	1
	2	Rapid field testing of mercury pollution by designed fluorescent biosensor and its cells-alginate hydrogel-based paper assay显示文摘With increasing industrial activities,mercury has been largely discharged into environment and caused serious environmental problems.The growing level of mercury pollution has become a huge threat to human health due to its significant biotoxicity.Therefore,the simple and fast means for on-site monitoring discharged mercury pollution are highly necessary to protect human beings from its pernicious effects in time.Herein,a'turn off'fluorescent biosensor(mCherry L199 C)for sensing Hg2+was successfully designed based on direct modification of the chromophore environment of fluorescent protein mCherry.For rapid screening and characterization,the designed variant of mCherry(mCherry L199 C)was directly expressed on outer-membrane of Escherichia coli cells by cell surface display technique.The fluorescent biosensor was characterized to have favorable response to Hg2+at micromole level among other metal ions and over a broad pH range.Further,the cells of the fluorescent biosensor were encapsulated in alginate hydrogel to develop the cells-alginate hydrogel-based paper.The cells-alginate hydrogel-based paper could detect mercury pollution in 5 min with simple operation process and inexpensive equipment,and it could keep fluorescence and activity stable at 4℃ for 24 hr,which would be a high-throughput screening tool in preliminarily reporting the presence of mercury pollution in natural setting.	Yanan Zheng Liudan Wei Linwei Duan Fangfang Yang Guixiang Huang Tianyi Xiao Min Wei Yanling Liang Huiting Yang Zhipeng Li Dan Wang	2021	Journal of Environmental Sciences2021，33，8：	0

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