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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | Exosomal PD-L1 harbors active defense function to suppress T cell killing of breast cancer cells and promote tumor growth显示文摘 | Yi Yang Chia-Wei Li Li-Chuan Chan Yongkun Wei Jung-Mao Hsu Weiya Xia Jong-Ho Cha Junwei Hou Jennifer L. Hsu Linlin Sun Mien-Chie Hung | 2018 | Cell Research2018，28，8： | 35 |
| 3 | Palmitoylation stabilizes PD-L1 to promote breast tumor growth显示文摘Dear Editor,PD-L1 is a well-known transmembrane protein, which is highly expressed on many types of cancer cells. By binding to its receptor PD-1 on T cells, PD-L1 significantly inhibits T cells activation and activity, and thus plays a pivotal role in driving the escape of tumor cells from immune surveillance.^1. | Yi Yang Jung-Mao Hsu Linlin Sun Li-Chuan Chan Chia-Wei Li Jennifer L. Hsu Yongkun Wei Weiya Xia Junwei Hou Yufan Qiu Mien-Chie Hung | 2019 | Cell Research2019，29，1： | 14 |
| 4 | Biosynthesis of poly-3-hydroxybutyrate with a high molecular weight by methanotroph from methane and methanol显示文摘Poly-3-hydroxybutyrate (哲学学士) 能被细菌的各种各样的种类生产。在可能的碳来源之中,甲烷和甲醇能是为哲学学士的生产的合适的底层。甲烷不仅象天然气,而且作为简历气体便宜、丰富。甲醇能也处于一些条件维持 methanotrophic 活动。methanotrophic 紧张 Methylosinus trichosporium IMV3011 能在一个简短非无菌的过程与甲烷和甲醇积累哲学学士。液体甲醇(0.1%) 被增加改进甲烷的氧化。研究用饮料烧瓶被执行。耕作在二个阶段被执行:在除了在批文化的必要营养素(铵,硝酸盐,磷,铜,铁(III ) ,镁或乙烯肼 tetraacetate (EDTA )) 的条件下面的一个连续生长阶段和一个哲学学士累积阶段。细胞的最合适的生长时间是 144 h,这被发现。然后,为第二个阶段的一个优化文化条件被决定,在哪个 PHB 集中能是增加到 0.6 g/L 的大部分。为了增加,哲学学士满意的、柠檬性的酸作为 tricarboxylic 酸周期(TCA ) 的一个禁止者被增加。柠檬性的酸为 PHB 累积是有利的,这被发现,并且 PHB 收益被增加到 40%(w/w ) 从在滋养的缺乏耕作以后的 12% 的起始的收益(w/w ) 。生产的哲学学士具有与直到 1.5 × 1 的分子的重量很高质量 0 6 Da。 | Yingxin Zhang Jiaying Xin Linlin Chen Hao Song Chungu Xia | 2008 | Journal of Natural Gas Chemistry2008，17，1： | 5 |
| 5 | Adenosine A 2A R modulates cardiovascular function by activating ERK1/2 signal in the rostral ventrolateral medulla of acute myocardial ischemic rats显示文摘 | Meiyan Jiang Chengrong Zhang Jin Wang Jun Chen Chunmei Xia Dongshu Du Na Zhao Yinxiang Cao Linlin Shen Danian Zhu | 2011 | Life Sciences2011，，5： | 4 |
| 6 | Effects of tillage managements and maize straw returning on soil microbiome using 16S rDNA sequencing显示文摘Agricultural practices could affect bacterial diversity and community structure by altering soil physical and chemical properties. Straw returning and tillage practices are widely used in agriculture, however, the effects of these agricultural practices on microbiomes are still unclear. In the present study, we compared the 18 bacterial communities of soil with different straw returning and tillage treatment combinations. The V3-V4 regions of the 16S ribosomal RNA were amplified and analyzed by high-throughput sequencing technology. The results showed that the bacterial communities were consistently dominated by Acidobacteria, Proteobacteria, Actinobacteria, and Chloroflexi. Short-term straw returning and tillage practices significantly altered the diversity, relative abundance and functions of the soil microbiome. Soil subjected to rotary tillage and straw returning (RTS) combination possessed the highest bacterial diversity and lowest ratio of G+/G- bacteria, indicating that RTS could be an efficient integrated management system to improve microbiome in the short term. Double verifications based on relative abundance and network analysis, revealed close relationships of Mycobacterium and Methylibium with RTS, indicating they could serve as biomarkers for RTS. Investigating microbial changes under different agricultural practices will provide valuable foundations for land sustainable utilization and increase crop yields. | Xinyao Xia Piaopiao Zhang Linlin He Xingxing Gao Weijun Li Yuanyuan Zhou Zongxin Li Hui Li Long Yang | 2019 | Journal of Integrative Plant Biology2019，61，6： | 3 |
| 7 | Breaking the linear correlations for enhanced electrochemical nitrogen reduction by carbon-encapsulated mixed-valence Fe_(7)(PO_(4))_(6)显示文摘Electrochemical nitrogen reduction(NRR)is deemed as a consummate answer for the traditional Haber–Bosch technology.Breaking the linear correlations between adsorption and transition-state energies of intermediates is vital to improve the kinetics of ammonia synthesis and obtain a less energy-intensive process.Herein,carbon-encapsulated mixed-valence Fe_(7)(PO_(4))_(6) was prepared and applied as an electrocatalyst for high-efficiency NRR.A dramatic faradaic efficiency(FE)of 36.93%and an NH_(3) production rate of 13.1μg h^(-1) mg_(cat)^(-1) were obtained at-0.3 V versus RHE,superior to nearly all Fe-based catalysts.Experiments and DFT calculations revealed that the superior performance was ascribed to the synergistic effect of mixed-valence iron pair,which braked the linear correlations to improve the kinetics of ammonia from collaborative hydrogenation and*NH_(3) separation.This work proves the feasibility of mixedvalence catalysts for nitrogen reduction and thus opening a new avenue towards artificial nitrogenfixation catalysts. | Lehui Ma Fanfan Xu Linlin Zhang Zhongfen Nie Kai Xia Mingxia Guo Mingzhu Li Xin Ding | 2022 | Journal of Energy Chemistry2022，31，8： | 1 |
| 8 | Analysis of the temporal and spatial distribution of water quality in China's major river basins, and trends between 2005 and 2010显示文摘 | Jinjian LI Xiaojie MENG Yan ZHANG Juan LI Linlin XIA Hongmei ZHENG | 2015 | Frontiers of Earth Science2015，9，3： | 1 |
| 9 | Assessment of land degradation in Inner Mongolia between 2000 and 2020 based on remote sensing data显示文摘Achieving land degradation neutrality(LDN)worldwide is a significant target of the Sustainable Development Goal(SDG15.3).Inner Mongolia,as a typical dryland region in northern China,has carried out several large-scale ecological restoration programs to combat land degradation.However,there is a lack of comprehensive assess-ment of its land degradation situation after ecological programs implementation,which is of great significance to supporting SDG15.3 in China.This study analyzed the land degradation situation using the improved SDG15.3.1 calculation framework based on fine resolution data in Inner Mongolia from 2000 to 2020,and finally compre-hensively evaluated the land status of the whole region and those subject to ecological programs.The results show that net land restoration proportion of various ecological project regions and whole region continues to increase.The scope of the Grain for Green Program(GGP)had the largest proportion of net land restoration while the Natural Reserve Program(NRP)had the lowest proportion from 2000 to 2020.The net land restoration area of Inner Mongolia during 2000-2010 and 2010-2020 was 35,800 km 2 and 65,300 km 2,respectively.Overall,Inner Mongolia has achieved statistically zero growth in land degradation under the governance of ecological restora-tion programs.Therefore,reasonable planning,well monitoring,and timely assessment of ecological restoration programs are crucial to support SDG15.3. | Linlin Zhao Kun Jia Xin Liu Jie Li Mu Xia | 2023 | Geography and Sustainability2023，4，2： | 1 |
| 10 | Natural interface-mediated self-assembly of graphene-isolatednanocrystals for plasmonic arrays construction and personalized information acquisition显示文摘As Interface mediated self-assembly of nanocrystals provide excellent strategy for sensing,catalysis or photonics,the construction of innovative interfaces and development of versatile strategies for nanocrystal synthesis are urgently needed.Herein,latent fingerprints(LFPs),the most common markers for human identity,are used as naturally accessible interface for organization of graphene isolated nanocrystals(GINs).Excitingly,the selective adsorption of GINs on lipidic ridge provides a universal approach for the in-situ construction of the plasmonic arrays.Such system with intrinsic chrominance and Raman signal enables the high resolution colorimetric and surfaced-enhanced Raman spectroscopy(SERS)dual-mode imaging,which can detail the structures of the LFPs from 1st to 3rd level even the LFPs are shielded.Furthermore,the interface can be constructed on diverse materials by a simple finger-pressing process and the densely packed arrays can serve as superior SERS substrate for label-free,non-invasive acquisition of molecule information especially residues in LFPs.The combination of chemical composition with detailed structures efficiently recognizes the human identity and could help link it to a crime scene.Overall,the LFPs can act as natural platform for interface mediated localized assembly and personalized information acquisition for forensic science or precise medicine. | Shen Wang Tianhuan Peng Shengkai Li Linlin Wang Liang Zhang Zhiwei Yin Xin Xia Xinqi Cai Xiaoxu Cao Long Chen Zhuo Chen Weihong Tan | 2022 | Nano Research2022，15，10： | 1 |
| 11 | Specific protein expression in a rat model of early focal cerebral ischemia:Fluorescent two-dimensional difference gel electrophoresis显示文摘BACKGROUND: The use of fluorescent two-dimensional difference gel electrophoresis (2D-DIGE) has been shown to compensate for the shortcomings of conventional two-dimensional gel electrophoresis, such as poor repeatability and large systematic errors. However, little information is presently available regarding the use of 2D-DIGE to investigate mechanisms of ischemic cerebrovascular diseases. Plasma and body fluids have been utilized in proteomic technology to study ischemic cerebrovascular diseases. OBJECTIVE: To perform proteomic analysis of fresh rat brain tissue in peripheral ischemic regions using 2D-DIGE 6 hours after middle cerebral artery occlusion (MCAO), and to identify specific proteins closely associated with early ischemic cerebrovascular diseases. DESIGN, TIME AND SETTING: Proteomics-based, randomized, controlled, animal experiment was performed at the Laboratories of Neurology and Proteomics, Jilin University between January and April 2006. MATERIALS: 2, 3, 5-triphenyl tetrazolium chloride was purchased from Sigma, USA. Ettan DALTSix system, DeCyder DIA V5.0 differential analysis software, and Ettan matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) were purchased from Amersham Bioscience, Sweden. METHODS: Eight healthy, male, Wistar rats were randomized to experimental and control groups, with four rats in each group. In the experimental group, rat models of focal cerebral ischemia were established by MCAO. In the control group, the internal and external carotid arteries were exposed and then immediately sutured, and the remaining procedures were identical to the experimental group. MAIN OUTCOME MEASURES: At 6 hours after cerebral ischemia, protein expression in the peripheral ischemia region of the experimental group was compared with the control group using 2D-DIGE. Protein spots that exhibited statistical differences between experimental and control groups with > 1.4 attributable risk were screened using DeCyder DIA V5.0 differential analysis software. Differential proteins were identified using MALDI-TOF-MS. RESULTS: Triphenyl tetrazolium chloride staining results revealed pink, normal brain tissue and white, ischemic brain tissue, suggesting successful MCAO establishment. The average matching rate of four 2D-DIGE gels was 92.4%. There were (1 758 ± 43) protein spots on each gel, with similar distribution modes. At 6 hours after focal cerebral ischemia, 13 protein spots exhibited marked expression changes, including significantly increased (n = 7) and decreased (n = 6) expression (P < 0.05). MALDI-TOF-MS results revealed two differential protein spots: α-tubulin and heat shock protein 27, which were significantly decreased in the experimental group compared with the control group (P < 0.05). CONCLUSION: Thirteen protein spots with expression changes were revealed by 2D-DIGE proteomics technology. Of them, α-tubulin and heat shock protein 27 expressions were markedly decreased during the early stage of cerebral ischemia. These two proteins were presumed to be proteins associated with early ischemic cerebrovascular diseases. | Xueling Ma Wei Yang Xinmei Jiang Fuchun Li Xia Li Linlin Ye Kangding Liu | 2010 | Neural Regeneration Research2010，5，3： | 0 |
| 12 | A facile strategy for large-scale production of 2D nanosheets exfoliated by three-roll milling显示文摘Two-dimensional(2D)nanomaterials,such as graphene,MoS_(2),and MAX,have attracted increasing research attention in recent years due to their unique structural and performance advantages.However,their complex production processes and equipment requirements are significant issues affecting their widespread use.Here,with an exfoliation strategy using three-roll milling,we present a simple,cost-effective,and extensible method to produce multilayer graphene,BN,MoS_(2),and Ti_(3)AlC_(2) nanosheets.The roller and phenolic resin created three kinds of forces on the layered 2D materials,i.e.,shear forces,compressive forces,and adhesive forces,which exfoliated layered materials from their edges and surfaces into nanosheets.Subsequently,the exfoliated materials were ultrasonically washed with alcohol,treated with ultrasonic vibration,and centrifuged to obtain 2D nanomaterials.The easy operation and high yield are attractive for research based on the construction of high-performance 2D nanosheet-based devices at low cost.Herein,the obtained multilayer graphene and MoS_(2) nanosheets were used as anode materials of sodium/potassium-ion batteries,respectively,to test their electrochemical properties.Better performances are obtained compared with their primary bulk materials. | Xin Wang Yang Xia Juntong Huang Yao Su Zhi Chen Linlin Chen Zhaohui Wu Zhijun Feng Huiyong Yang Xibao Li | 2024 | Journal of Advanced Ceramics2024，13，1： | 0 |
| 13 | Identification and Expression Analysis of Abscisic Acid Signal Transduction Genes in Hemp Seeds显示文摘Abscisic acid(ABA)is involved in regulating diverse biological processes,but its signal transduction genes and roles in hemp seed germination are not well known.Here,the ABA signaling pathway members,PYL,PP2C and SnRK2 gene families,were identified from the hemp reference genome,including 7 CsPYL(pyrab-actin resistance1-like,ABA receptor),8 CsPP2CA(group A protein phosphatase 2c),and 7 CsSnRK2(sucrose nonfermenting1-related protein kinase 2).The content of ABA in hemp seeds in germination stage is lower than that in non-germination stage.Exogenous ABA(1 or 10μM)treatment had a significant regulatory effect on the selected PYL,PP2C,SnRK2 gene families.CsAHG3 and CsHAI1 were most significantly affected by exogenous ABA treatment.Yeast two-hybrid experiments were performed to reveal that CsPYL5,CsSnRK2.2,and CsSnRK2.3 could interact with CsPP2CA7 and demonstrate that this interaction was ABA-independent.Our results indicated that CsPYL5,CsSnRK2.2,CsSnRK2.3 and CsPP2CA7 might involve in the ABA signaling transduction pathway of hemp seeds during the hemp seed germination stages.This study suggested that novel genetic views can be brought into investigation of ABA signaling pathway in hemp seeds and lay the foundation for further exploration of the mechanism of hemp seed germination. | Cong Hou Kang Ning Xiuye Wei Yufei Cheng Huatao Yu Haibin Yu Xia Liu Linlin Dong | 2023 | Phyton-International Journal of Experimental Botany2023，92，7： | 0 |
| 14 | A novel feature susceptibility approach for a PEMFC control system based on an improved XGBoost-Boruta algorithm显示文摘Data-driven modelling methods are being developed in the quest to achieve more accurate performance prediction of protons exchange membrane fuel cell (PEMFC) systems in response to their complicated physicochemical phenomena. However, there is little research in this field detailing the pre-processing and selection of balance of plants (BOP) features for the input layer of system performance prediction at different current densities. Furthermore, most of the previous research applies neural networks based on simulation data rather than real-time bench or vehicle operation datasets which leads to low robustness and unreliable practical results. This paper details the application of a novel algorithm denoted XGBoost-Boruta, which utilises the combination of an ensemble learning approach and a wrapping approach, to improve the robustness of feature selection and to increase the accuracy and robustness of PEMFC system performance prediction. By introduction of the Z score and shadow features to eliminate the randomness of conventional ensemble learning methods, seven key controllable BOP variables of the hydrogen anode, air cathode and cooling subsystems are selected as the original input variables to determine their dependency on the stack voltage. Two case studies are presented for verification and validation of the proposed algorithm based on the real-time dataset of bench experimental data and data obtained from heavy truck operation at current densities ranging from 100 to 1500 mA/cm2. The feature selection strategy, based on the proposed XGBoost-Boruta algorithm, largely decreases the RMSE by 23.8% and 14.1% and the R^(2) increases by 0.06 and 0.04 of both the bench experimental and the heavy truck validation datasets respectively. | Xinjie Yuan Fujun Chen Zenggang Xia Linlin Zhuang Kui Jiao Zhijun Peng Bowen Wang Richard Bucknall Konrad Yearwood Zhongjun Hou | 2023 | Energy and AI2023，12，2： | 0 |
| 15 | Interactions of Microplastics and Methane Seepage in the Deep-Sea Environment显示文摘Microplastics(MPs)are important exempla of the Anthropocene and are exerting an increasing impact on Earth’s carbon cycle.The huge imbalance between the MPs floating on the marine surface and those that are estimated to have been introduced into the ocean necessitates a detailed assessment of marine MP sinks.Here,we demonstrate that cold seep sediments,which are characterized by methane fluid seepage and a chemosynthetic ecosystem,effectively capture and accommodate small-scale(<100μm)MPs,with 16 types of MPs being detected.The abundance of MPs in the surface of the sediment is higher in methane-seepage locations than in non-seepage areas.Methane seepage is beneficial to the accumulation,fragmentation,increased diversity,and aging of MPs.In turn,the rough surfaces of MPs contribute to the sequestration of the electron acceptor ferric oxide,which is associated with the anaerobic oxidation of methane(AOM).The efficiency of the AOM determines whether the seeping methane(which has a greenhouse effect 83 times greater than that of CO_(2)over a 20-year period)can enter the atmosphere,which is important to the global methane cycle,since the deep-sea environment is regarded as the largest methane reservoir associated with natural gas hydrates. | Jing-Chun Feng Zhifeng Yang Wenliang Zhou Xingwei Feng Fuwen Wei Bo Li Chuanxin Ma Si Zhang Linlin Xia Yanpeng Cai Yi Wang | 2023 | Engineering2023，，10： | 0 |
| 16 | Characteristics of Seismic Sequences and Early Tendency Judgment for Yutian M_S7.3 Earthquake in 2014显示文摘The basic parameters,seismogenic structure and seismic sequences characteristics of the Yutian MS7. 3 earthquake on February 12,2014 are introduced and compared to the Yutian MS7. 3 earthquake in 2008. The results show that the MS5. 4 earthquake is regarded as an immediate foreshock of the Yutian MS7. 3 main shock. The frequency of strong aftershock sequences was low and their number declined quickly,and the maximum aftershock was a MS5. 7 earthquake. According to analysis of the historical earthquake sequence type,and parameter of h-value,b-value and energy release ratio between main shock and sequence etc.,we found the preliminary conclusion that the Yutian MS7. 3 earthquake sequence in 2014 was a foreshock-main shock-aftershock type. | Song Chunyan Zhang Linlin Nie Xiaohong Xia Aiguo Wei Yunyun | 2015 | Earthquake Research in China2015，29，3： | 0 |
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