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| 1 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 2 | The experimental study of genetic engineering human neural stem cells mediated by lentivirus to express multigene显示文摘 | Cai PQ Tang X Lin YQ Martin O Sun GY Xu L Yang YK Zhou TH | 2006 | 中国生物学文摘2006，20，5： | 6 |
| 3 | Synergistic control of sex hormones by 17β-HSD type 7: a novel target for estrogen-dependent breast cancer显示文摘17β-hydroxysteroid dehydrogenase(17β-HSD)type 1 is known as a critical target to block the final step of estrogen production in estrogen-dependent breast cancer.Recent confirmation of the role of dyhydroxytestosterone(DHT)in counteracting estrogeninduced cell growth prompted us to study the reductive 17β-HSD type 7(17β-HSD7),which activates estrone while markedly inactivatingDHT.The role ofDHTin breast cancer cell proliferation isdemonstratedby its independent suppression of cell growthin the presence of a physiological concentration of estradiol(E2).Moreover,an integral analysis of a large number of clinical samples in Oncomine datasets demonstrated the overexpression of 17β-HSD7 in breast carcinoma.Inhibition of 17β-HSD7 in breast cancer cells resulted in a lower level of E2 and a higher level of DHT,successively induced regulation of cyclinD1,p21,Bcl-2,and Bik,consequently arrested cell cycle in the G0/G1 phase,and triggered apoptosis and auto-downregulation feedback of the enzyme.Such inhibition led to significant shrinkage of xenograft tumors with decreased cancer cell density and reduced 17β-HSD7 expression.Decreased plasma E2 and elevated plasma DHT levels were also found.Thus,the dual functional 17β-HSD7 is proposed as a novel target for estrogen-dependent breast cancer by regulating the balance of E2 andDHT.Thisdemonstrates aconceptual advance on the general belief that the major role of this enzyme is in cholesterol metabolism. | Xiaoqiang Wang Catherine Gérard Jean-Francois Thériault Donald Poirier Charles J.Doillon Sheng-Xiang Lin | 2015 | Journal of Molecular Cell Biology2015，7，6： | 2 |
| 4 | Effects of different exercise modes on mineralization, structure, and biomechanical properties of growing bone显示文摘 | Huang TH Lin SC Chang FL | 2003 | J Appl Physiol2003，95，1： | 1 |
| 5 | Early identification of lepto- spirosis as an ignored cause of muhiple organ dysfunction syndrome 显示文摘 | Yang HY Yen TH Lin CY | 2012 | Shock2012，38，1： | 1 |
| 6 | Arsenic and lipid peroxidation in patients with blactfoot disease显示文摘 | Lin TH | 1995 | Bull of Environm Contam Toxicol1995，54，4： | 1 |
| 7 | Expression and prognostic role of tumor suppressor gene PTEN/MMAC1/TEP1 in hepatocellular carcinoma显示文摘 | Hu TH Huang CC Lin PR | 2003 | Cancer2003，1597，8： | 1 |
| 8 | General cone-beam reconstruction algorithm显示文摘 | Wang G Lin TH Cheng PC | 1993 | IEEE Transactions on Medical Imaging1993，12，3： | 1 |
| 9 | PPARgamma inhibits osteogenesis via the down-regulation of the expression of COX-2 and iNOS in rats显示文摘 | Lin TH Yang RS Tang CH | 2007 | Bone2007，41，4： | 1 |
| 10 | Wireless Integrated Network Sensors (WINS)显示文摘 | Asada G Bhatti I Lin TH | 1999 | Proceedings of SPIE -The International Society for Optical Engineering1999，3673，： | 1 |
| 11 | Determination of time-dependent accumulation of d-lactate in the streptozotocin-induced diabetic rat kidney by column-switching HPLC with fluorescence detection显示文摘 | Lin MH Chen HY Liao TH | 2011 | J Chromatogr B Analyt Technol Biomed Life Sci2011，879，29： | 1 |
| 12 | Telbivudine and adefovir combination therapy for patients with chronic lamivudine-resistant hepatitis B virus infections显示文摘 | Lin MT Chou YP Hu TH | 2014 | Arch Virol2014，159，1： | 1 |
| 13 | Relationship of progesterone/estradiol ratio on day of hCG administration and pregnancy outcomes in high responders undergoing in vitro fertilization显示文摘 | Lee FK Lai TH Lin TK | | 0，，04： | 1 |
| 14 | Chronic inflammation in bioma- terialinduced periprosthetie osteolysis: NF-KB as a therapeutic target 显示文摘 | Lin TH Tamaki Y Pajarinen J | 2014 | Aeta Biomater2014，10，1： | 1 |
| 15 | Long non - coding RNA AOC4J5 suppresses hepatocellular carcinoma metastasis by enhan- cing vimentin degradation and inhibiting epithelial - mesenchymal transition 显示文摘 | Wang TH Lin YS Chen Y | 2015 | Oncotarget2015，6，23： | 1 |
| 16 | Mechanism of concentration - dependent induction of heme oxygenase - 1 by resveratrol in human aortic smooth muscle cells 显示文摘 | Juan SH Cheng TH Lin HC | 2005 | Biochem Pharmacol2005，69，1： | 1 |
| 17 | Ananalytical investigation of the mechanics of spinal instrumentation显示文摘 | Geol VK Kim YE Lin TH | 1988 | Spine1988，13，9： | 1 |
| 18 | Preliminary feasibility study of FTIR microscopic mapping system for the rapid detection of the composited components of prostatic calculi 显示文摘 | Hsu TH Lin SY Lin CC | 2011 | Urol Res2011，39，： | 1 |
| 19 | Blood eosinophilia, corticoadre- hal insufficiency and eosinophilic cystitis 显示文摘 | Lin HH Yen TH Huang CC | 2008 | Urol Int2008，80，2： | 1 |
| 20 | Can iterative reconstruction improve imaging quality for lower radiation CT perfusion? Initial experience 显示文摘 | Lin C J Wu TH Lin C H | 2013 | AJNR2013，34，： | 1 |
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