|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Knockdown of survivin gene expression by RNAi induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721显示文摘AIM: To investigate the survivin gene expression in human hepatocellular carcinoma cell line SMMC-7721 and the effects of survivin gene RNA interference (RNAi) on cell apoptosis and biological behaviors of SMMC-7721 cells.METHODS: Eukaryotic expression vector of survivin gene RNAi and recombinant plasmid pSuppressorNeo-survivin (pSuNeo-SW), were constructed by ligating into the vector,pSupperssorNeo (pSuNeo) digested with restriction enzymes Xba I and Sa/I and the designed double-chain RNAi primers. A cell model of SMMC-7721 after treatment with RNAi was prepared by transfecting SMMC-7721 cells with the lipofectin transfection method. Strept-avidinbiotin-complex (SABC) immunohistochemical staining and RT-PCR were used to detect survivin gene expressions in SMMC-7721 cells. Flow cytometry was used for the cell cycle analysis. Transmission electron microscopy was performed to determine whether RNAi induced cell apoptosis, and the method of measuring the cell growth curve was utilized to study the growth of SMMC-7721 cells before and after treatment with RNAi.RESULTS: The eukaryotic expression vector of survivin gene RNAi and pSuNeo-SW, were constructed successfully. The expression level of survivin gene in SMMC-7721 cells was observed. After the treatment of RNAi, the expression of survivin gene in SMMC-7721 cells was almost absent,apoptosis index was increased by 15.6%, and the number of cells was decreased in G2/M phase and the cell growth was inhibited.CONCLUSION: RNAi can exert a knockdown of survivin gene expression in SMMC-7721 cells, and induce apoptosis and inhibit the growth of carcinoma cells. | Sheng-QuanCheng Wen-LiangWang WeiYan Qing-LongLi LiWang Wen-YongWang | 2005 | World Journal of Gastroenterology2005，11，5： | 42 |
| 2 | Pancreatic cancer mortality in China(1991-2000)显示文摘AIM: To describe the mortality rate of pancreatic cancer and its distribution in China during the period of 1991-2000.METHODS: Based on the data of demography and death collected through China′s Disease Surveillance Point System (DSPS) over the period of 1991-2000, the distribution of death rate of pancreatic cancer was described in terms of age group, gender, calendar year, rural/urban residence and administrative district.RESULTS: A total of 1 619 death cases attributed to pancreatic cancer (975 men and 644 women) were reported by DSPS during 1991-2000. The reported, adjusted and agestandardized mortality rates increased from 1.46, 1.75, and 2.18 per 100 000 populations in 1991 to 2.38, 3.06, and 3.26per 100 000 populations in 2000. The majority (69.62 %) of the deaths of pancreatic cancer were seen in the age group of 60 years and older. The mortality rate was higher in men than in women, but the male to female death rate ratios decreased during the 10 years. Our data also showed that the death rate of pancreatic cancer in urban areas was about 2-4 fold higher than that in rural areas, and in Northeast and East China, the death rates were higher than those in the other 5 administrative districts.CONCLUSION: The death rate due to pancreatic cancer was rising during the period of 1991-2000 and the peak mortality of pancreatic cancer might arrive in China. | LiWang Gong-HuanYang Xing-HuaLu Zheng-JingHuang HuiLi | 2003 | World Journal of Gastroenterology2003，9，8： | 38 |
| 3 | Ginkgo biloba extract reverses CCI4-induced liver fibrosis in rats显示文摘AIM: To study the reversing effect of Ginkgo biloba extract (GbE) on established liver fibrosis in rats. METHODS: Following confirmation of CCI4-induced liver fibrosis, GbE or saline was administrated to the rats for 4 weeks. The remaining rats received neither CCI 4 norGbE as normal control. The four groups were compared in terms of serum enzymes, tissue damage, expression of αSMA and tissue inhibitor-1 of metalloproteinase (TIMP-1) and metalloproteinase-1 (MMP-1). RESULTS: Compared with saline-treated group, liver fibrosis rats treated with GbE had decreased serum total bilirubin (P<0.01) and aminotransferase levels (P<0.01) and increased levels of serum albumin (P<0.01). Microscopic studies revealed that the livers of rats receiving GbE showed allieviation in fibrosis (P<0.05) as well as expression of αSMA (P<0.01). The liver collagen and reticulum contents were lower in rats treated with GbE than saline-treated group (P<0.01). RT-PCR revealed that the level of TIMP-1 decreased while the level of MMP-1 increased in GbE group. CONCLUSION: Administration of GbE improved CCI4-induced liver fibrosis. It is possibly attributed to its effect of inhibiting the expression of TIMP-1 and promoting the apoptosis of hepatic stellate cells. | Yan-JunLuo Jie-PingYu Zhao-HongShi LiWang | 2004 | World Journal of Gastroenterology2004，10，7： | 20 |
| 4 | Molecular basis for the selective and ABA-independent inhibition of PP2CA by PYL13显示文摘PYR1/PYL/RCAR 家庭蛋白质(PYL ) 是描绘得好的 abscisic 酸(骆驼毛的织物) 受体。在在 Arabidopsis thaliana 的 14 个 PYL 成员之中, PYL13 是无反应的骆驼毛的织物,它的功能留下了逃犯。这里,我们证明 PYL13 有选择地禁止独立于骆驼毛的织物的 PP2CA 的磷酸酶活动。PYL13-PP2CA 建筑群的水晶结构,它在 2.4 Å 坚定;分辨率,阐明在 PP2CA 和 PYL13 之间的特定的识别的分子的基础。除了在 PYL 和 PP2Cs 之间的正规相互作用,一个额外的接口被识别在附近包含一个元素一以前在 PP2CA 的 uncharacterized CCCH 锌手指(ZF ) 主题。顺序强风识别了另一 56 包含 ZF PP2Cs,所有哪个从植物。结构也为 PYL13 的 ABA 无反应揭示分子的决定因素。最后,生物化学的分析建议那 PYL13 可以有 PYL10 的 hetero-oligomerize。这二 PYL 在他们 PP2Cs 的各自的骆驼毛的织物无关的抑制反对对方。生物化学、结构的研究在植物的压力反应提供重要卓见进 PYL13 的函数并且为 PYL13 的未来 biotechnological 应用程序安装一个基础。 | WenqiLi #x0002A LiWang #x0002A XinleiSheng #x0002A ChuangyeYan RuiZhou JingHang PingYin NiengYan | 2013 | Cell Research2013，，12： | 10 |
| 5 | Genetic regulation by non-coding RNAs显示文摘Large scale cDNA sequencing and genome tiling array studies have shown that around 50% of genomic DNA in humans is transcribed, of which 2% is translated into proteins and the remaining 98% is non-coding RNAs (ncRNAs). There is mounting evidence that these ncRNAs play critical roles in regulating DNA structure, RNA expression, protein translation and protein functions through multiple genetic mechanisms, and thus affect normal development of organisms at all levels. Today, we know very little about the regulatory mechanisms and functions of these ncRNAs, which is clearly essential knowledge for understanding the secret of life. To promote this emerging research subject of critical importance, in this paper we review (1) ncRNAs' past and present, (2) regulatory mechanisms and their functions, (3) experimental strategies for identifying novel ncRNAs, (4) experimental strategies for investigating their functions, and (5) methodologies and examples of the application of ncRNAs. | QI Liwang, LI Xinmin, ZHANG Shougong & AN Daochang Laboratory of Cell Biology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China Functional Genomics Facility, Division of Biological Science, University of Chicago, Chicago 60637, USA China National Center for Biotechnology and Development, Ministry of Science and Technology, Beijing 100081, China | 2006 | Science China(Life Sciences)2006，49，3： | 10 |
| 6 | Lipid metabolism disturbances and AMPK activation in prolonged propofol-sedated rabbits under mechanical ventilation显示文摘瞄准: 探索位于 propofol 注入症候群(PRIS ) 下面的机制,潜在地致命的复杂并发症在延长 propofol 期间注入。 | WeiJiang Zheng-boYang Quan-hongZhou XiangHuan LiWang | 2012 | Acta Pharmacologica Sinica2012，33，1： | 7 |
| 7 | Toxic activity of a protein complex purified from Xenorhabdus nematophila HB310 to Plutella xylostella larvae显示文摘Xenorhabdus nematophila,属于家庭 Enterobacteriaceae 的克否定的 proteobacterium 并且与土壤 entomopathogenic 线虫共生地联系了, Steinernema carpocapsae,对大量昆虫病原。有杀虫的活动的蛋白质建筑群从房间 ofX 被孤立。外面腌和本国的 polyacrylamide 胶化电气泳动(页) 的 nematophilaHB310 紧张使用方法。七多肽变化 50250 kDa 被钠 dodecyl 硫酸盐(SDS ) 很好与蛋白质建筑群(命名 Xnpt ) 分开其五被帮助矩阵的激光 desorption-time-of-flight 团 spectrometry (MALDI-TOFMS ) 作为 XptA2, xptC1, XptB1, GroEL 和假想蛋白质识别的 -PAGE, 。Xnpt 显示出高口头的毒力到有菱形斑纹的蛾(DBM ) 的幼虫, Plutella xylostellaL。(鳞翅目, Plutellidae ) 作为它对第二和第三中间形态的中部的致命的集中(LC50 ) ,幼虫分别地在 72 h 是 331.45 ng/mL 和 553.59 ng/mL。喂 Xnpt 的 DBM 幼虫的组织学的分析在 midgut 上显示出广泛的组织病理学说的效果。生物化学的分析显示 Xnpt 显著地在 midgut 禁止了三重要的酶的活动。总的来说,我们的数据证明蛋白质建筑群孤立 fromX。nematophilaHB310 由破坏 midgut 纸巾并且禁止 midgut 朊酶活动导致了昆虫的 antifeedant 和死亡。 | Qin-Ying Wang Zi-Yan Nangong Jun Yang Ping Song Yi Wang Liwang Cui Long Cui | 2012 | Insect Science2012，19，3： | 7 |
| 8 | Experiment research on grain drying process in the heat pump assisted fluidized beds显示文摘A heat pump assisted fluidized bed grain drying experimental system was developed. Based on this system, a serial of ex- periments was performed under four kinds of air cycle conditions. According to the experimental analysis, an appropriate drying medium-air cycle for the heat pump assisted fluidized bed drying equipment was decided, which is different from the commonly used heat pump assisted drying system. The experimental results concerning the drying operation performance of the new system show that the averaged coefficient of performance (COP) can reach more than 2.5. The economical evaluation was performed and the power consumption for removing a kilogram water from grains was about 0.485 kW.h/kg (H2O), which shows its reasonable commercial efficiency and great application potentiality in future market. | JingYang LiWang FiXiang LigeTong HuaSu | 2004 | Journal of University of Science and Technology Beijing2004，11，4： | 7 |
| 9 | 恶性疟原虫抗药性测定方法及其影响因素显示文摘本文就疟原虫抗药性测定方法近几年的研究进展及其在测定中的影响因素加以综述。对抗药性测定方法,如传统的微量测试法和同位素测定法,以及近几年发展起来的利用HRP Ⅱ和LDH酶联免疫法、利用SYBR Green I等标记的荧光法和流式细胞仪法进行了介绍。文内还分析了血清、红细胞压积和原虫起始密度、原虫时期和状态等因素对测定值的影响。 | 赵振(综述) Liwang Cui 吴兰鸥 杨照青(审校) | 2010 | 中国热带医学2010，10，9： | 6 |
| 10 | Population pharmacokinetic model of valproate and prediction of valproate serum concentrations in children with epilepsy显示文摘AIM: Using sparse data of valproate (VPA) serum concentrations to build a population pharmacokinetic (PPK) model of VPA in Chinese children with epilepsy and to predict serum concentrations for new patients using a Bayesian approach. METHODS: Two hundred epileptic children, whose VPA serum concentrations were collected, were divided randomly into two groups (A and B, n=100 each). The PPK parameter values of group A were calculated to establish a PPK Model by using the NPEM Program of USC*PACK software. Based on it, VPA serum concentrations of group B were predicted with the Bayesian Fitting Program of the USC*PACK software. To assess the accuracy and precision of prediction, a paired-comparisons t-test was run between predicted and ob- served concentrations, and then the mean prediction error (MPE), mean square prediction error (MSPE), root mean square prediction error (RMSPE), and coincidence rates for different percentages of prediction error were all calculated. RESULTS: Optimum PPK parameters were: Ka, 2.522±2.743 h-1; Vs, 0.329±0.496 L/kg; and Kel, 0.0438±0.0384 h-1. For group B, there was no significant difference between predicted and observed concentrations. MPE was -0.43 mg/L, MSPE was 115.40 (mg/L)2, and RMSPE was 5.47 mg/L. The coincidence rates for percent- ages of prediction error, which were less than 5 %, 10 %, 15 %, 20 %, 25 %, and 30 %, were 62 %, 74 %, 82 %, 85 %, 89 %, and 93 %, respectively. CONCLUSION: A PPK model of VPA in epileptic children was successfully established. Based on it, VPA serum concentrations can be predicted accurately with a Bayesian approach. | De-chunJIANG LiWANG | 2004 | Acta Pharmacologica Sinica2004，25，12： | 5 |
| 11 | Effects of extracellular iron concentration on calcium absorption and relationship between Ca^(2+) and cell apoptosis in Caco-2 cells显示文摘AIM: To determine the method of growing small intestinal epithelial cells in short-term primary culture and to investigate the effect of extracellular iron concentration ([Fe3+]) on calcium absorption and the relationship between the rising intracellular calcium concentration ([Ca2+]i) and cell apoptosis in human intestinal epithelial Caco-2 cells. METHODS: Primary culture was used for growing small intestinal epithelial cells. [Ca2+]i was detected by a confocal laser scanning microscope. The changes in [Ca2+]i were represented by fluorescence intensity (FI). The apoptosis was evaluated by flow cytometry.RESULTS: Isolation of epithelial cells and preservation of its three-dimensional integrity were achieved using the digestion technique of a mixture of collagenase Ⅺ and dispase Ⅰ. Purification of the epithelial cells was facilitated by using a simple differential sedimentation method. The results showed that proliferation of normal gut epithelium in vitro was initially dependent upon the maintenance of structural integrity of the tissue. If 0.25% trypsin was used for digestion, the cells were severely damaged and very difficult to stick to the Petri dish for growing. The Fe3+ chelating agent desferrioxamine (100, 200 and 300 μmol/L) increased the FI of Caco-2 cells from 27.50±13.18 (control,n = 150) to 35.71±13.99 (n = 150, P<0.01), 72.19±35.40 (n = 150, P<0.01) and 211.34±29.03 (n = 150, P<0.01) in a concentration-dependent manner. There was a significant decrease in the FI of Caco-2 cells treated by ferric ammonium citrate (FAC, a Fe3+ donor; 10, 50 and 100 μmol/L). The FIvalue of Caco-2 cells treated by FAC was 185.85±33.77 (n = 150, P<0.01), 122.73±58.47 (n = 150, P<0.01), and 53.29±19.82 (n = 150, P<0.01), respectively, suggesting that calcium absorption was influenced by [Fe3+]. Calcium ionophore A23187 (0.1, 1.0 and 10 μmol/L) increased the FI of Caco-2 cells from 40.45±13.95 (control, n = 150) to 45.19±21.95 (n = 150, P<0.01), 89.87±43.29 (n = 150,P<0.01) and 104.64±51.07 (n = 150, P<0.01) in a concentration-dependent manner. The positive apoptotic cell number of the Caco-2 cells after being treated with indicating that the increase in the positive apoptotic cell~ increased from 0.32% to 0.69%, 0.90% and 1.10%, number was positively correlated with [Ca2+]i.CONCLUSION: Ca2+ absorbability is increased with the decrease of extracellular iron concentration Fe3+ and hindered with the increase of Fe3+ consistence out of them. Furthermore, increase of [Ca2+]i can induce apoptosis in Caco-2 cells. | LiWang QingLi Xiang-LinDuan Yan-ZhongChang | 2005 | World Journal of Gastroenterology2005，11，19： | 4 |
| 12 | 青蒿素类药物治疗间日疟的研究进展显示文摘疟疾作为一种致死性很高的全球性寄生虫病,一直被全世界所关注,疟疾的控制也被列入全球三大公共卫生问题之一。由于长期抗疟药的使用,目前恶性疟原虫对主要抗疟药物普遍产生抗药性,青蒿素类药物是在氯喹、奎宁抗药性产生后的主要替代治疗药。而对于间日疟原虫引起的疟疾的治疗,却与恶性疟原虫大不相同,本文针对间日疟的青蒿素治疗现状及进展情况进行了综述。 | 徐馨 赵书仙 Liwang Cui 杨照青 | 2014 | 中国热带医学2014，14，12： | 3 |
| 13 | A genome-wide association study uncovers a critical role of the RsPAP2 gene in red-skinned Raphanus sativus L.显示文摘Radish(Raphanus sativus L.)taproot contains high concentrations of flavonoids,including anthocyanins(ATCs),in redskinned genotypes.However,little information on the genetic regulation of ATC biosynthesis in radish is available.A genome-wide association study of radish red skin color was conducted using whole-genome sequencing data derived from 179 radish genotypes.The R2R3-MYB transcription factor production of anthocyanin pigment 2(PAP2)gene was found in the region associated with a leading SNP located on chromosome 2.The amino acid sequence encoded by the RsPAP2 gene was different from those of the other published RsMYB genes responsible for the red skin color of radish.The overexpression of the RsPAP2 gene resulted in ATC accumulation in Arabidopsis and radish,which was accompanied by the upregulation of several ATC-related structural genes.RsPAP2 was found to bind the RsUFGT and RsTT8 promoters,as shown by a dual-luciferase reporter system and a yeast one-hybrid assay.The promoter activities of the RsANS,RsCHI,RsPAL,and RsUFGT genes could be strongly activated by coinfiltration with RsPAP2 and RsTT8.These findings showed the effectiveness of GWAS in identifying candidate genes in radish and demonstrated that RsPAP2 could(either directly or together with its cofactor RsTT8)regulate the transcript levels of ATC-related genes to promote ATC biosynthesis,facilitating the genetic enhancement of ATC contents and other related traits in radish. | Lianxue Fan YanWang Liang Xu Mingjia Tang Xiaoli Zhang Jiali Ying Cui Li Junhui Dong Liwang Liu | 2020 | Horticulture Research2020，7，1： | 2 |
| 14 | Occurrence and a screening‐level risk assessment of human pharmaceuticals in the Pearl River system, South China显示文摘 | Jian‐LiangZhao Guang‐GuoYing You‐ShengLiu FengChen Ji‐FengYang LiWang Xiao‐BingYang Jenny L.Stauber Michael St. J.Warne | 2010 | Environmental Toxicology and Chemistry2010，，6： | 2 |
| 15 | Effect of ginkgo biloba extract on livers in aged rats显示文摘AIM: To investigate the protective effect of ginkgo biloba extract (GBE) on livers of aged rats and the associated mechanisms.METHODS: Two-mo- and 20-mo-old rats were treated with GBE/saline for 3 mo. Liver tissue samples from 5-moold rats treated with saline (group Y) and 23-mo-old rats treated with GBE (group E) or saline (group N) were used for histopathological examinations (hematoxylin-eosin and Masson staining, Lipofuscin staining-Schmorl staining) and determination of expression of tissue inhibitor-1 of metalloproteinase (TIMP-1) and the level of malondialdehyde (MDA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). Blood samples were collected for determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and albumin.RESULTS: Microscopic studies with Masson staining revealed mild liver fibrosis in aged rats (group N), while the livers of aged rats receiving GBE (group E) showed amelioration in fibrosis (2.2±0.1 vs 2.8±0.1, P<0.01) and deposition of lipofuscin (33.7±5.3 vs 62.8±5.7, P<0.01).The expression of TIMP-1 and the level of liver MDA (1.0±0.1 vs 1.2±0.2, P<0.05) also decreased but the activity of GPx (97.1±15.3 vs 61.8±14.5, P<0.01) increased in group E. Compared with group Y, the level of liver MDA (0.8±0.1 vs 1.2±0.2, P<0.01), lipofuscin (32.4±6.0 vs 62.8±5.7, P<0.01) and TIMP-1 expression were increased,while the activity of GPx (103.2±17.6 vs61.8±14.5, P<0.01)and SOD (16.7±4.4 vs 11.8±3.9, P<0.05) was decreased in group N. There was no difference in liver function among these three groups.CONCLUSION: GBE has protective effects on aging liver.The possible mechanisms might be its antioxidant activity and inhibition of TIMP-1 expression. | Shang-ZhenHuang Yan-JunLuo LiWang Ke-YinCai | 2005 | World Journal of Gastroenterology2005，11，1： | 2 |
| 16 | A state-of-the-art review of mechanical characteristics and cracking processes of pre-cracked rocks under quasi-static compression显示文摘The mechanical characteristics and failure behavior of rocks containing flaws or discontinuities have received wide attention in the field of rock mechanics.When external loads are applied to rock materials,stress-induced cracks would initiate and propagate from the flaws,ultimately leading to the irreversible failure of rocks.To investigate the cracking behavior and the effect of flaw geometries on the mechanical properties of rock materials,a series of samples containing one,two and multiple flaws have been widely investigated in the laboratory.In this paper,the experimental results for pre-cracked rocks under quasistatic compression were systematically reviewed.The progressive failure process of intact rocks is briefly described to reveal the background for experiments on samples with flaws.Then,the nondestructive measurement techniques utilized in experiments,such as acoustic emission(AE),X-ray computed tomography(CT),and digital image correlation(DIC),are summarized.The mechanical characteristics of rocks with different flaw geometries and under different loading conditions,including the geometry of pre-existing flaws,flaw filling condition and confining pressure,are discussed.Furthermore,the cracking process is evaluated from the perspective of crack initiation,coalescence,and failure patterns. | Liwang Liu Haibo Li Xiaofeng Li | 2022 | Journal of Rock Mechanics and Geotechnical Engineering2022，14，6： | 2 |
| 17 | CC and CX3 C chemokines differentially interact with the N terminus of the human cytomegalovirus-encoded US28 receptor 显示文摘 | Casarosa P Waldhoer M LiWang PJ | 2005 | J Biol Chem2005，280，5： | 1 |
| 18 | Modal Analysis of Thread off Failure of Oil Round Thread Casing Connection显示文摘The dynamic modal model analysis of the oil casing connection is done by finite element method (FEM), and the first nature frequency, the second nature frequency, the vibration mode shape and the time domain of the node displacement and the element stress variation in the thread connection are gotten. The Von.Mises stresses of the connection in the static and dynamic state are also studied. The results of calculation and analysis show: (1) because the maximum of static stress is at the coupling thread end of connection, the connection begins to thread off at the coupling thread end, which is in accord with the results of the thread off experiment in laboratory; (2) because the first nature frequency is very high, the probability of casing connection to be damaged from vibration is little; (3) the sho- ck dynamic load makes casing connection begin to thread off at the tube thread end. | LiWang, Yong Zang, Zhaoneng Chen, Dechun Tong, Jiaxiang Zou 1) Shanahai Jiaotong University Shanghai 200030 China 2) Mechanical Engineering School. University of Science and Technology Beijing. Beijing 100083. China | 2001 | Journal of University of Science and Technology Beijing2001，8，4： | 1 |
| 19 | The binding surface and affinity of monomeric and dimeric chemokine macrophage inflammatory protein 1 beta for various glycosaminoglycan disaccharides 显示文摘 | McCornack MA Cassidy CK LiWang PJ | 2003 | J Biol Chem2003，278，3： | 1 |
| 20 | Prevalence of chronic kidney disease in China:a cross-sectional survey显示文摘 | Zhang LiWang F Wang L etc | 2012 | Lancet2012，379，9818： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员