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| 1 | Molecular basis for the selective and ABA-independent inhibition of PP2CA by PYL13显示文摘PYR1/PYL/RCAR 家庭蛋白质(PYL ) 是描绘得好的 abscisic 酸(骆驼毛的织物) 受体。在在 Arabidopsis thaliana 的 14 个 PYL 成员之中, PYL13 是无反应的骆驼毛的织物,它的功能留下了逃犯。这里,我们证明 PYL13 有选择地禁止独立于骆驼毛的织物的 PP2CA 的磷酸酶活动。PYL13-PP2CA 建筑群的水晶结构,它在 2.4 Å 坚定;分辨率,阐明在 PP2CA 和 PYL13 之间的特定的识别的分子的基础。除了在 PYL 和 PP2Cs 之间的正规相互作用,一个额外的接口被识别在附近包含一个元素一以前在 PP2CA 的 uncharacterized CCCH 锌手指(ZF ) 主题。顺序强风识别了另一 56 包含 ZF PP2Cs,所有哪个从植物。结构也为 PYL13 的 ABA 无反应揭示分子的决定因素。最后,生物化学的分析建议那 PYL13 可以有 PYL10 的 hetero-oligomerize。这二 PYL 在他们 PP2Cs 的各自的骆驼毛的织物无关的抑制反对对方。生物化学、结构的研究在植物的压力反应提供重要卓见进 PYL13 的函数并且为 PYL13 的未来 biotechnological 应用程序安装一个基础。 | WenqiLi #x0002A LiWang #x0002A XinleiSheng #x0002A ChuangyeYan RuiZhou JingHang PingYin NiengYan | 2013 | Cell Research2013，，12： | 10 |
| 2 | Recognition of methylated DNA by TAL effectors显示文摘 | DongDeng PingYin ChuangyeYan XiaojingPan XinqiGong ShiqianQi TianXie MagdyMahfouz Jian-KangZhu NiengYan YigongShi | 2012 | Cell Research2012，22，10： | 4 |
| 3 | Dissecting the membrane-microtubule sensor in grapevine defence显示文摘Specific populations of plant microtubules cooperate with the plasma membrane to sense and process abiotic stress signals,such as cold stress.The current study derived from the question,to what extent this perception system is active in biotic stress signalling.The experimental system consisted of grapevine cell lines,where microtubules or actin filaments are visualised by GFP,such that their response became visible in vivo.We used the bacterial elicitors harpin(inducing cell-death related defence),or flg22(inducing basal immunity)in combination with modulators of membrane fluidity,or microtubules.We show that DMSO,a membrane rigidifier,can cause microtubule bundling and trigger defence responses,including activation of phytoalexin transcripts.However,DMSO inhibited the gene expression in response to harpin,while promoting the gene expression in response to flg22.Treatment with DMSO also rendered microtubules more persistent to harpin.Paradoxically,Benzylalcohol(BA),a membrane fluidiser,acted in the same way as DMSO.Neither GdCl_(3),nor diphenylene iodonium were able to block the inhibitory effect of membrane rigidification on harpin-induced gene expression.Treatment with taxol stabilised microtubule against harpin but amplified the response of PAL transcripts.Therefore,the data support implications of a model that deploys specific responses to pathogen-derived signals. | Pingyin Guan Wenjing Shi Michael Riemann Peter Nick | 2021 | Horticulture Research2021，8，1： | 0 |
| 4 | Anti-microtubule activity of the traditional Chinese medicine herb Northern Ban Lan(Isatis tinctoria)leads to glucobrassicin显示文摘Traditional Chinese medicine(TCM)belongs to the most elaborate and extensive systems of plant-based healing.The herb Northern Ban Lan(Isatis tinctoria)is famous for its antiviral and anti-inflammatory activity.Although numerous components isolated from I.tinctoria have been characterized so far,their modes of action have remained unclear.Here,we show that extracts from I.tinctoria exert antimicrotubular activity.Using time-lapse microscopy in living tobacco BY-2(Nicotiana tabacum L.cvBrightYellow2)cellsexpressing greenfluorescentprotein-tubulin,weuse activity-guided fractionation to screen out the biologically active compounds of I.tinctoria.Among 54 fractions obtained from either leaves or roots of I.tinctoria by methanol(MeOH/H_(2)O 8:2),or ethyl acetate extraction,one specific methanolic root fraction was selected,because it efficiently and rapidly eliminated microtubules.By combination of further purification with ultrahigh-performance liquid chromatography and high-resolution tandem mass spectrometry most of the bioactivity could be assigned to the glucosinolate compound glucobrassicin.Glucobrassicin can also affect microtubules and induce apoptosis in HeLa cells.In the light of these findings,the antiviral activity of Northern Ban Lan is discussed in the context of microtubules being hijacked by many viral pathogens for cell-to-cell spread. | Pingyin Guan Jianning Zhou Sergey Girel Xin Zhu Marian Schwab Kunxi Zhang Qiyan Wang-Müller Laurent Bigler Peter Nick | 2021 | Journal of Integrative Plant Biology2021，63，12： | 0 |
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