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| 1 | Identify lymphatic metastasis-associated genes in mouse hepatocarcinoma cell lines using gene chip显示文摘AIM: In order to obtain lymphogenous metastasisassociated genes, we compared the transcriptional profiles of mouse hepatocarcinoma cell lines Hca-F with highly lymphatic metastasis potential and Hca-P with low lymphatic metastasis potential.METHODS: Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA. In vitro transcription double-stranded cDNA was labeled with biotin (i.e. biotin-labeled cRNA, used as the probe). The cRNA probes hybridized with Affymetrix GeneChip() MOE430A (containing 22 690 transcripts, including 14 500 known mouse genes and 4 371 ESTs) respectively and the signals were scanned by the GeneArray Scanner. The results were then analyzed by bioinformatics.RESULTS: Out of the 14 500 known genes investigated,110 (0.8%) were up regulated at least 23 fold. Among the total 4 371 ESTs, 17 ESTs (0.4%) (data were not presented) were up regulated at least 23 fold. According to the Gene Ontology and TreeView analysis, the 110genes were further classified into two groups: differential biological process profile and molecular function profile.CONCLUSION: Using high-throughput gene chip method,a large number of genes and their cellular functions about angiogenesis, cell adhesion, signal transduction, cell motility, transport, microtubule-based process, cytoskeleton organization and biogenesis, cell cycle, transcription,chaperone activity, motor activity, protein kinase activity,receptor binding and protein binding might be involved in the process of lymphatic metastasis and deserve to be used as potential candidates for further investigation.Cyclin D1, Fosl1, Hsp47, EGFR and AR, and Cav-1 are selected as the possible candidate genes of the metastatic phenotype, which need to be validated in later experiments.ESTs (data were not presented) might indicate novel genes associated with lymphatic metastasis. Validating the function of these genes is helpful to identify the key or candidate gene/pathway responsible for lymphatic metastasis, which might be used as the diagnostic markers and the therapeutic targets for lymphatic metastasis. | BoSong Jian-WuTang BoWang Xiao-NanCui LiHou LuSun Li-MinMao Chun-HuiZhou YueDu Li-HuiWang Hua-XinWang Ren-ShuZheng LeiSun | 2005 | World Journal of Gastroenterology2005，11，10： | 19 |
| 2 | Construction and selection of subtracted cDNA library of mouse hepatocarcinoma cell lines with different lymphatic metastasis potential显示文摘AIM: In order to elucidate the molecular mechanism of lymphatic metastasis of hepatocarcinoma, we detected the difference of gene expression between mouse hepatocarcinoma cell lines Hca-F and Hca-P with different lymphatic metastasis potential.METHODS: cDNA of Hca-F cells was used as a tester and cDNA of Hca-P cells was used as a driver, cDNAs highly expressed in Hca-F cells were isolated by the suppression subtractive hybridization (SSH) method. The isolated cDNA was cloned into T/A cloning vector. The ligation products were transformed into DH5 α competent cells. Individual dories were randomly selected and used for PCR amplification.Vector DNA from positive clones was isolated for sequencing.RESULTS: There were 800 positive clones in amplified subtracted cDNA library. Random analysis of 160 clones with PCR showed that 95% of the clones contained 100-700 bp inserts. Analysis of 20 sequenced cDNA clones randomly picked from the SSH library revealed 4 known genes(mouse heat shock protein 84 ku, DNA helicase, ribosomal protein S13 ,ethanol induced 6 gene) and 3 expressed sequence tags (ESTs). Four cDNAs showed no homology and presumably represent novel genes.CONCLUSION: A subtracted cDNA library of differentially expressed genes in mouse heptocarcinoma cell lines with different lymphatic metastasis potential was successfully constructed with SSH and T/A cloning techniques. The library is efficient and lays a solid foundation for searching new lymphatic metastasis related genes. The expression of mouse heat shock protein gene, DNA helicase and other 4 novel gene may be different between mouse heptocarcinoma cell lines with different lymphatic metastasis potential. | LiHou Jan-WuTang Xiao-NanCui BoWang BoSong LeiSun | 2004 | World Journal of Gastroenterology2004，10，16： | 9 |
| 3 | Screening difFerentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis显示文摘AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis.METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed.RESULTS: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes.CONCLUSION: SSH is a useful technique to detect differentially expressioned genes and an effective method to clone novel genes. | Xiao-NanCui Jian-WuTang LiHOU BoSong LiLi Ji-WeiLiu | 2005 | World Journal of Gastroenterology2005，11，12： | 2 |
| 4 | Shape and terminal velocity of slurry drops in air显示文摘 | Lihou D A | 1996 | Birmingham Univ Chem Eng1996，，16： | 1 |
| 5 | Arrest of Replication Forks by Drug-stabilized topoisomerase Ⅰ-DNA Cleavable Complexes as a Mechanism of Cell Killing by Camptothecin显示文摘 | Hsiang YH Lihou MG Liu LF | 1989 | Cancer Res1989，49，18： | 1 |
| 6 | Uncertainties in above ground tree biomass estimation显示文摘Models of above-ground tree biomass have been widely used to estimate forest biomass using national forest inventory data.However,many sources of uncertainty affect above-ground biomass estimation and are challenging to assess.In this study,the uncertainties associated with the measurement error in independent variables(diameter at breast height,tree height),residual variability,variances of the parameter estimates,and the sampling variability of national inventory data are estimated for five above-ground biomass models.The results show sampling variability is the most significant source of uncertainty.The measurement error and residual variability have negligible effects on forests above-ground biomass estimations.Thus,a reduction in the uncertainty of the sampling variability has the greatest potential to decrease the overall uncertainty.The power model containing only the diameter at breast height has the smallest uncertainty.The findings of this study provide suggestions to achieve a trade-off between accuracy and cost for above-ground biomass estimation using field work. | Lihou Qin Shengwang Meng Guang Zhou Qijing Liu Zhenzhao Xu | 2021 | Journal of Forestry Research2021，32，5： | 1 |
| 7 | Arrest of replicating forks by drug-stabilized topoisomerase Ⅰ-DNA cleavable complexes as a mechanism of cell killing by camptothecin 显示文摘 | Lihou MG Lin LF | 1989 | Cancer Res1989，49，： | 1 |
| 8 | Arrest of replication forks by drug-stabilized topoisomerase I-DNA cleavable complexes as a mechanism of cell killing by camptothecin显示文摘 | Hsiang YH Lihou MG Liu LF | 1989 | Cancer Res1989，49，18： | 1 |
| 9 | Use of intravesicalvalrubicin in clinical practice for treatment of nonmuscleinvasivebladder cancer, including carcinoma in situ of the bladder显示文摘 | Cookson MS Chang SS Lihou C | 2014 | TherAdv Urol2014，6，5： | 1 |
| 10 | Provenance of the Sardana Flysch, eastern Swiss Alps : example of high resolution heavy mineral analysis applied to an ultrastable assemblage 显示文摘 | Lihou J C Mange-Rajetzky M A | 1996 | Sedimentary Geology1996，105，： | 1 |
| 11 | Arrest of replication forks by drug-stabilized topoisomerase I-DNA cleavable complexes as a mechanism of cell killing by camptothecin显示文摘 | HSIANG Y H LIHOU M G LIU L F | 1989 | Cancer Res1989，49，： | 1 |
| 12 | Provenance of the Sardona Flysch, eastern Swiss Alps: Example of high resolution heavy-mineral analysis applied to an ultra-stable assemblage 显示文摘 | Lihou J C Mange-Rajetzky M A | 1996 | Sedimentary Geology1996，105，： | 1 |
| 13 | Arrest of replication forks by drug-stabilized topoisomerase I-DNA cleavable complexes as a mechanism of cell killing by camptothecin 显示文摘 | Hsiang YH Lihou MG Liu LF | 1989 | Cancer Res1989，49，： | 1 |
| 14 | Provenance of the Sardona Flysch, eastern Swiss Alps: example of high-resolution heavy mineral analysis applied to an ultrastable assemblage显示文摘 | Lihou J C Mange-Rajetzky M A | 1996 | Sedimentary Geology1996，105，34： | 1 |
| 15 | Provenance of the Sardona Flysch, eastern Swiss Alps: Example of high resolution heavy mineral analysis applied to an ultrastable assemblage显示文摘 | Lihou J C Mange-Rajetzky M A | 1996 | Sedimentary Geology1996，105，： | 1 |
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