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| 1 | Identification of differentially expressed genes in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis显示文摘AIM: To identify genes differentially expressed in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis. METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with low potential of lympho- genous metastasis Hca-P and its synogenetic cell line Hca-F with high metastatic potential was constructed by suppression subtracted hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed. RESULTS: Fifteen differentially expressed cDNA fragments of Hca-P were obtained which revealed 8 known genes, 4 expressed sequence tags (ESTs) and 3 cDNAs showed no homology. CONCLUSION: Tumor metastasis is an incident involving multiple genes. SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes. | Xiao-Nan Cui Jian-Wu Tang Li Hou Bo Song Li-Ying Ban | 2006 | World Journal of Gastroenterology2006，12，42： | 5 |
| 2 | 烯酯酰辅酶A水解酶1基因在小鼠肝癌细胞株中的表达及其与增殖和迁移能力的关系显示文摘目的 研究烯酯酰辅酶A水解酶1( ECH1)在小鼠肝癌高、低淋巴转移Hca-F/Hca-P细胞株中的表达及ECH1基因表达下调后对Hca-F细胞增殖和迁移能力的影响.方法 采用免疫荧光方法检测ECH1在小鼠肝癌细胞株中的表达.构建pGPU6/GFP/Neo-shRNA-ECH1表达载体,稳定转染至高淋巴转移Hca-F细胞中干扰ECH1基因的表达,通过CCK8法检测肿瘤细胞的增殖能力,Transwell小室检测肿瘤细胞的迁移能力,并应用流式细胞仪检测肿瘤细胞的细胞周期情况.结果 ECH1在小鼠肝癌细胞株中主要表达于细胞质,且ECH1在Hca-F细胞的表达强于其在Hca-P细胞的表达.ECH1表达下调后,Hca-F细胞的增殖能力明显下降.Transwell小室检测穿膜细胞数pGPU6/GFP/Neo-shRNA-ECH1转染Hca-F组(27.07±17.49)较阴性对照组(72.38±18.83)和Hca-F组(59.06±30.33)明显减少,细胞周期S期pGPU6/GFP/Neo-shRNA-ECH1转染Hca-F组(86.1%)较阴性对照组(75.8%)和Hca-F组(66.2%)停留细胞数明显增多,G1期pGPU6/GFP/Neo-shRNA-ECH1转染Hca-F组(9.4%)较阴性对照组(24.2%)和Hca-F组(30.3%)停留细胞数明显减少.结论 ECH1可能在肿瘤淋巴转移中发挥作用,抑制ECH1的表达可有效降低体外高淋巴转移肿瘤细胞的增殖和迁移能力. | 张军 唐建武 宋美英 王波 李荣宽 侯力 黄玉红 王梅 宋波 金艳玲 | 2011 | 中华病理学杂志2011，40，12： | 2 |
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