|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Microstructure of Al-Si Alloys Rapidly Solidified from the Different Temperature Melts显示文摘Hypo- and hyper-eutectic Al-Si alloys were rapidly solidified from the low or high temperature melt using water quenching and melt-spinning technique. The differences in microstructure of rapid solidification alloys were studied by scanning and transmission electron microscopy,and X-ray diffraction methods. The primary silicon in the Al-22(wt pct)Si alloy quenched in room temperature water from high temperature melt was smaller than that quenched from low temperature melt. Compared with the ribbons spun from low temperature melt, the ribbons spun from high temperature melt had finer Al-rich and Si-rich phase particles in either Al-7(wt pct)Si or Al-18(Wt pct)Si alloy. The mechanism was explained by the nature of the liquid | Gui, MC Jia, J Song, GS Li, QC | 1999 | Journal of Materials Science & Technology1999，15，3： | 7 |
| 3 | Cerebral phaeohyphomycosis-a cure at what lengths? 显示文摘 | Li DM de Hoog GS | 2009 | The Lancet Infectious Diseases2009，9，6： | 1 |
| 4 | Cordyceps sinensis preserves in- testinal mucosai barrier and may be an adjunct therapy in endo- toxin-induced sepsis rat mode显示文摘 | Gu GS Ren JA Li GW | 2015 | Int J ClinExp Med2015，8，5： | 1 |
| 5 | Ultraviolet B exposure of peripheral blood mononuclear ceils of patients with systemiclupus erythematosus inhibits DNA methylation显示文摘 | Wang GS Zhang M Li XP | 2009 | Lupus2009，18，12： | 1 |
| 6 | Screening of mimetic peptides for CD14binding site with LBP and antiendotoxin activity of mimetic peptidein vivo and in vitro显示文摘 | Xu Z Qian GS Li Q | 2009 | Inflamm Res2009，58，1： | 1 |
| 7 | On albonoursin-a substance accompanying the antibiotics nystatin and albofungin显示文摘 | Rozenfeld GS Rostovtseva LI Baikina VM | 1963 | Antibiotiki1963，8，： | 1 |
| 8 | The influence of surgical sites on early postoperative hypoxemia in adults undergoing elective surgery显示文摘 | Xue FS Li BW Zhang GS | 1999 | Anesth Analg1999，88，： | 1 |
| 9 | A nero strategy of iontophoresis for hyperhidrosis显示文摘 | Shen JL Lin GS Li WM | 1990 | J Am Acad Dermatol1990，22，21： | 1 |
| 10 | Health Literacy and health status of Korean-Chinese elderly people living in Yanbian,China显示文摘 | LI CY Lee O Shin GS | 2009 | J Korean Acad Nurs2009，39，3： | 1 |
| 11 | Valproate pretreatment protects dopaminergic neurons from LPS-induced neurotoxicity in rat primary midbrain cultures: role of microglia显示文摘 | Peng GS Li G Tzeng NS Chen PS Chuang DM Hsu YD Yang S Hong JS | 2006 | 中国生物学文摘2006，20，10： | 1 |
| 12 | Effect of Deanxit,Omeprazole and Mosapride in patients of intractable gastroesophageal reflux disease显示文摘 | Li CH Sha ZK Shun GS | | 0，，06： | 1 |
| 13 | Reliability and validity of a physical activity scale among urban pregnant women in Eastern China显示文摘 | Jiang H He GS Li M | | 0，，： | 1 |
| 14 | Norwalk-like virus 95/96-US strain is a major cause of gastroenteritis outbreaks in Australia显示文摘 | White PA Hansman GS Li A | 2002 | J Med Virol2002，68，1： | 1 |
| 15 | Protective effects of daviditin A against endothelial damage induced by lysophosphatidylcholine显示文摘 | Jiang DJ Jiang JL Tan GS Du YH Xu KP Li YJ | 2003 | Naunyn Schmiedebergs Arch Pharmacol2003，367，： | 1 |
| 16 | Interleukin-1 induces major phenotypic changes in skin microvascular endothelial cells显示文摘 | Romero LI Zhang DN Herron GS | 1997 | J Cell Physiol1997，173，： | 1 |
| 17 | Norwalk-like vinls 95/96-US strain is a major cause of gastroenteritis outbreaks in Australia显示文摘 | White PA Hansman GS Li A | 2002 | J Med Virol2002，68，1： | 1 |
| 18 | An acquired translocation in JAK2 Val617Phe-negative essential thrombocythemia associated with autosomal spread of X-inactivation显示文摘 | Vassiliou GS Campbell PJ Li J | | 0，，08： | 1 |
| 19 | Norwalk-like virus 95/96-US strain is a major cause of gastroenteritis outbreaks in Australia显示文摘 | Hansman GS Li A | 2002 | J Med Virol2002，68，1： | 1 |
| 20 | Mdm2 di- rects the ubiquitination ofbeta-arrestin-se- questered cAMP phosphodiesterase-4D5 显示文摘 | Li X Baillie GS Houslay MD | 2009 | Biol Chem2009，284，16： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员