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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Recognition Interactions of Metal-complexing Imprinted Polymer显示文摘Molecularly imprinted polymer, exhibiting considerable enantioselectivity for L-mandelic acid, was prepared using metal coordination-chelation interaction. By evaluating the recognition characteristics in the chromatographic mode, the recognition interactions were proposed: specific and nonspecific metal coordination-chelation interaction and hydrophobic interaction were responsible for substrate binding on metal-complexing imprinted polymer; while the selective recognition only came from specific metal coordination-chelation interaction and specific hydrophobic interaction. | Liu, Y Ding, GS De Wang, J | 2005 | Chinese Chemical Letters2005，16，6： | 5 |
| 3 | Effects of chronic treatment with honokiol in spontaneously hypertensive rats 显示文摘 | Zhang GS Wang R J Zhang HN | 2010 | Biol Pharm Bull2010，33，3： | 1 |
| 4 | The lethality of tentacle-only extract from jellyfish Cyanea capillata is primarily attributed to cardiotoxicity in anaesthetized SD rats 显示文摘 | Xiao L Liu GS Wang QQ | 2010 | Toxicon2010，55，4： | 1 |
| 5 | Hedgehog signaling main- tains a tumor stem cell compartment in multiple myeloma 显示文摘 | Peacock CD Wang Q Gesell GS | 2007 | Proc Natl Acad Sci U S A2007，104，10： | 1 |
| 6 | Construction of anti-breaking mod-els of the main veins of flue-cured to-bacco leaves and principal component analysis显示文摘 | WANG BY SUN TT ZHANG GS | 2011 | Agricultural Science&Tech-nology2011，12，16： | 1 |
| 7 | Ultraviolet B exposure of peripheral blood mononuclear ceils of patients with systemiclupus erythematosus inhibits DNA methylation显示文摘 | Wang GS Zhang M Li XP | 2009 | Lupus2009，18，12： | 1 |
| 8 | The effect of cultured autologous periodontal ligament cells on the healing of delayed autotransplanted dog's teeth 显示文摘 | Wang Y Cheung GS Xu X | 2010 | J Endod2010，36，2： | 1 |
| 9 | Identification of groundwater sources of a local-scale creep slope: Using environmental stable isotopes as tracers显示文摘 | Peng TR Wang CH Hsu SM Wang GS Su TW | 2010 | Journal of Hydrology2010，381，1： | 1 |
| 10 | Role of autophagy in Cisplatin resistance in ovarian cancer cells 显示文摘 | Wang J Wu GS | 2014 | Journal of Biological Chemistry2014，289，24： | 1 |
| 11 | The lethality of tentacle-only extract from jellyfish Cyanea capillata is primarily attributed to cardiotoxicity in anaesthetized SD rats显示文摘 | Xiao L Liu GS Wang QQ | 2010 | Toxicon2010，55，4： | 1 |
| 12 | Membrane water permeability related to antigen-presenting function of dendritic cells 显示文摘 | Wang GF Dong CL Tang GS | 2008 | Clin Exp Immunol2008，153，3： | 1 |
| 13 | Role of nitric oxide in immunological liver damage in mice显示文摘 | Wang GS Liu GT | 1995 | Biochem Phamacol1995，49，2： | 1 |
| 14 | The mammalian target of rapamycin regulates cholesterol biosynthetic gene expression and exhibits a rapamycin-resistant transcriptional profile 显示文摘 | Wang BT Ducker GS Barczak AJ | 2011 | Proc Nail Acad Sci USA2011，108，15： | 1 |
| 15 | MDCI is a mediator of the mammalian DNA damage checkpoint显示文摘 | Stewart GS Wang B Bignell CR | 2003 | Nature2003，421，6926： | 1 |
| 16 | Genetic alterations in hepatocellular carcinomas: association between loss of chromosome 4q and p53 gene mutations显示文摘 | Rashid A Wang JS Qian GS | 1999 | Br J Cancer1999，80，12： | 1 |
| 17 | Occupational exposures of air- borne trichloramine at indoor swimming pools in Taipei 显示文摘 | Chu TS Cheng SF Wang GS | 2013 | Sci Total Environ2013，，： | 1 |
| 18 | Retinoid quantification by HPLC/MSn显示文摘 | Wang Y Chang WY Prins GS | 2001 | J Mass Spectrom2001，36，8： | 1 |
| 19 | Role of nitric oxide in immunological liver damage in mice显示文摘 | Liu GT | 1995 | Biochem Pharmacol1995，49，9： | 1 |
| 20 | Generation and characterization of B7-H4/B7S1/B7x2 deficient mice显示文摘 | Suh WK Wang S Duncan GS | 2006 | Mol Cell Biol2006，26，17： | 1 |
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