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| 1 | Activation of nuclear factor-kappa B and effects of pyrrolidine dithiocarbamate on TNBS-induced rat colitis显示文摘AIM: To explore the changes of nuclear factor-kappa B (NF-κB) DNA-binding activity, the expression of intercellular adhesion molecule-1 (ICAM-1) regulated by NF-κB at various times and to evaluate the effects of pyrrolidine dithiocarbamate (PDTC) on trinitrobenzene sulfonic acid (TNBS)-induced rat colitis.METHODS: TNBS of 0.6 mL was mixed with ethanol of 0.3 mL solution and instilled into the lumen of the rat colon. The rat models were divided into 6 groups, which were killed at 24 h, 3, 7, 14, and 21 d after enema. Colonic inflammation and damage were assessed by macroscopical and histological criteria. Activity of NF-κB DNA-binding was analyzed by electrophoresis mobility shift assays (EMSA).Expression of ICAM-1 was detected by in situ hybridization (ISH) and immunohistochemistry (IH). Then various doses of PDTC were injected into rat abdomen 30 min before enema with TNBS/ethanol as pretreatment. The rats were killed 4 h after enema and the colonic inflammation,myeloperoxidase (MPO) activity, malondialdehyde (MDA)level, and DNA-binding activity of NF-κB were assessed.Finally, PDTC was injected intraperitoneally after colitis was induced. Changes of morphology were assayed.RESULTS: During the first week, hyperemia, hemorrhage,edema and ulceration of the colonic mucosa appeared with predominant infiltration of leukocytes. Neutrophils,macrophages, lymphocytes infiltrated in mucosa and submucosa 14 d later. Fibroblasts and granuloma-like structures were also obviously seen. The binding activity of NF-κB began to increase at 24 h time point and reached a peak at 14 d, then decreased but still was higher than control group at 21 d (P<0.01). Levels of tCAM-1 mRNA and protein significantly elevated at 24 h and the peak was at 21 d. Pretreatment with PDTC could attenuate the development of inflammation but not by reducing NF-κB activity. This attenuation of inflammation had a positive relationship with the dose of PDTC. PDTC at the dose of 100 mg/kg had no therapeutic effect after colitis was induced.CONCLUSION: NF-κB activation is an important event that may be involved in acute and chronic inflammation development and may contribute to self-protection against early inflammation damage. NF-κB also regulates ICAM-1expression during colonic inflammation. Pretreatment of PDTC may attenuate the inflammation development. But PDTC has no therapeutic effect after the colitis is induced. | KenChen You-MingLong HuiWang LeiLan Zhen-HeLin | 2005 | World Journal of Gastroenterology2005，11，10： | 13 |
| 2 | Acute kidney injury risk in patients with ST-segment elevation myocardial infarction at presentation to the ED显示文摘 | Rafaela Elizabeth Bayas Queiroz Leilane Siqueira Nobre de Oliveira Cláudio Alves de Albuquerque Caroline de Alencar Santana Patrícia Maia Brasil Luzia Layla Rodrigues Carneiro Alexandre Braga Libório | 2012 | American Journal of Emergency Medicine2012，，9： | 1 |
| 3 | Digital Printing with Food Color Jet Inks显示文摘 | Yu Leilan Yao Yun Yang Jiurui Yu Boling | 2003 | International Dyer2003，188，11： | 1 |
| 4 | Nursing allocation and adverse events or incidents in intensive care units 显示文摘 | Leilane A G Rafaela A Elaine M O | 2012 | Rev Esc Enferm USP2012，，： | 1 |
| 5 | Photoluminescence properties of CaTiO3:Eu3+ nanophosphor obtained by the polymeric precursor method显示文摘 | TATIANA M M LUCAS M DA R O LEILANE R M | 2014 | Materials Chemistry and Physics2014，145，12： | 1 |
| 6 | Nursing activities score (NAS) : A proposal for practical application in intensive care units显示文摘 | Leilane AG Katia GP Regina M | 2007 | Intensive and Critical Care Nursing2007，23，： | 1 |
| 7 | Bulk Dyeing Trials with Natural Tannic Acid and Gallic Acid 显示文摘 | Yu Leilan Yu Boling Chen Shuguang | 2004 | Intemational Dyer2004，，8： | 1 |
| 8 | Effects of the addition of microencapsulated omega-3 and rosemary extract on the technological and sensory quality of white pan bread显示文摘 | Leilane Costa De Conto Raquel Silveira | 2012 | Food Science and Technology2012，45，1： | 1 |
| 9 | Acute kidney injury risk in patients with ST-segment elevation myocardial infarction at presentation to the ED显示文摘 | Rafaela Elizabeth Bayas Queiroz Leilane Siqueira Nobre de Oliveira Cláudio Alves de Albuquerque Caroline de Alencar Santana Patrícia Maia Brasil Luzia Layla Rodrigues Carneiro Alexandre Braga Libório | 2012 | American Journal of Emergency Medicine2012，，9： | 1 |
| 10 | Combinational therapy with Myc decoy oligodeoxynucleotides encapsulated in nanocarrier and X-irradiation on breast cancer cells显示文摘The Myc gene is the essential oncogene in triple-negative breast cancer(TNBC).This study investigates the synergistic effects of combining Myc decoy oligodeoxynucleotides-encapsulated niosomes-selenium hybrid nanocarriers with X-irradiation exposure on the MDA-MB-468 cell line.Decoy and scramble ODNs for Myc transcription factor were designed and synthesized based on promoter sequences of the Bcl2 gene.The nanocarriers were synthesized by loading Myc ODNs and selenium into chitosan(Chi-Se-DEC),which was then encapsulated in niosome-nanocarriers(NISM@Chi-Se-DEC).FT-IR,DLS,FESEM,and hemolysis tests were applied to confirm its characterization and physicochemical properties.Moreover,cellular uptake,cellular toxicity,apoptosis,cell cycle,and scratch repair assays were performed to evaluate its anticancer effects on cancer cells.All anticancer assessments were repeated under X-ray irradiation conditions(fractionated 2Gy).Physicochemical characteristics of niosomes containing SeNPs and ODNs showed that it is synthesized appropriately.It revealed that the anticancer effect of NISM@Chi-Se-DEC can be significantly improved in combination with X-ray irradiation treatment.It can be concluded that NISM@Chi-Se-DEC nanocarriers have the potential as a therapeutic agent for cancer treatment,particularly in combination with radiation therapy and in-vivo experiments are necessary to confirm the efficacy of this nano-drug. | BEHROOZ JOHARI MILAD PARVINZAD LEILAN MAHMOUD GHARBAVI YOUSEF MORTAZAVI ALI SHARAFI HAMED REZAEEJAM | 2024 | Oncology Research2024，32，2： | 0 |
| 11 | 黄酮类化合物紫花牡荆素通过内质网应激上调死亡受体5促进TRAIL诱导的结肠癌细胞凋亡(英文)显示文摘Objective:The aim of this study was to explore the mechanisms by which the flavonoid casticin enhances tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in colon cancer cells. Methods:Human colon cancer HT-29 cells were treated with TRAIL or casticin. Cytotoxicity was examined by MTT assay, and apoptosis determined by morphological observation and flow cytometric analysis. Death receptor 5 (DR5), DR4, and endoplasmic reticulum (ER) stress response markers, including glucose regulating protein 78 (GRP78), activating transcription factor 4 (ATF4) and CHOP (CCAAT/enhancer binding protein homologous protein), were examined with western blot. Small interfering RNA (siRNA) transfection was employed to knock down CHOP. Results:HT-29 cells were resistance to TRAIL-induced apoptosis, but casticin, at subtoxic concentrations, potentiated HT-29 cells to TRAIL-induced apoptosis. Casticin up-regulated the expression of DR5 time-and dose-dependent manners, but had no effect on the expression of DR4. Also, casticin increased the levels of ER stress response markers (GRP78, ATF4 and CHOP) in a similar way to DR5. Knockdown of CHOP by specific siRNA, or salubrinal, an ER stress inhibitor, abolished the up-regulation of DR5 and enhancement of TRAIL-induced apoptosis by casticin. Conclusion:Casticin enhances TRAIL-induced apoptosis of colon cancer cells by ER stress-mediated up-regulation of DR5. | Sanyuan Tang Guangjin Yuan Zhengyang Yu Leilan Yin Hao Jiang | 2013 | The Chinese-German Journal of Clinical Oncology2013，12，6： | 0 |
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