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| 1 | Oxymatrine therapy for chronic hepatitis B:A randomized doubleblind and placebo-controlled multi-center trial显示文摘AIM: To evaluate the efficacy and safety of capsule oxymatrine in the treatment of chronic hepatitis B.METHODS: A randomised double-blind and placebocontrolled multicenter trial was conducted. Injection of oxymatrine was used as positive-control drug. A total of 216 patients with chronic hepatitis B entered the study for 24 weeks, of them 108 received capsule oxymatrine, 36 received injection of oxymatrine, and 72 received placebo.After and before the treatment, clinical symptoms, liver function, serum hepatitis B virus markers, and adverse drug reaction were observed.RESULTS: Among the 216 patients, six were dropped off,and 11 inconsistent with the standard were excluded.Therefore, the efficacy and safety of oxymatrine in patients were analysed. In the capsule treated patients, 76.47 % became normal in ALT level, 38.61% and 31.91% became negative both in HBV DNA and in HBeAg. In the injection treated patients, 83.33 % became normal in ALT level,43.33 % and 39.29 % became negative both in HBV DNA and in HBeAg. In the placebo treated patients, 40.00 % became normal in ALT level, 7.46 % and 6.45 % became negative both in HBV DNA and in HBeAg. The rates of complete response and partial response were 24.51% and 57.84 % in the capsule treated patients, and 33.33 % and 50.00 % in the injection treated patients, and 2.99 % and 41.79 % in the placebo treated patients, respectively.There was no significance between the two groups of patients, but both were significantly higher than the placebo. The adverse drug reaction rates of the capsule,injection and placebo were 7.77 %, 6.67 % and 8.82 %,respectively. There was no statistically significant difference among them.CONCLUSION: Oxymatrine is an effective and safe agent for the treatment of chronic hepatitis B. | Lun-GenLu Min-DeZeng Yi-MinMao Ji-QiangLi Mo-BinWan Cheng-ZhongLi Cheng-WeiChen Qing-ChunFu Ji-YaoWang Wei-MinShe XiongCai JunYe Xia-QiuZhou HuiWang Shan-MingWu Mei-FangTang Jin-ShuiZhu Wei-XiongChen Hui-QuanZhang | 2003 | World Journal of Gastroenterology2003，9，11： | 33 |
| 2 | Dose-dependent effects of genistein on bone homeo- stasis in rats' mandibular subchondral bone显示文摘Aim: To investigate the effect of genistein on bone homeostasis in mandibular subchondral bone of rats. Methods: Female SD rats were administered with genistein (10 and 50 mg/kg) or placebo by oral gavage for 6 weeks. Then the ani- mals were sacrificed, and histomorphology and micro-structure of mandibular condyle were examined using HE staining and micro-CT analysis, respectively. The expression levels of alkaline phosphatase (ALP), osteocalcin (OC), osteoprotegerin (OPG), the receptor activator of nuclear factor KB ligand (RANKL) and estrogen receptors (ERs) in mandibular condyle were detected using real-time PCR. Cultured osteoblasts were prepared from rat mandibular condyle for in in vitro study. The cells were treated with genistein (10-7 or 10-4 mol/L) for 48 h. The expression of the bone homeostasis-associated factors and estrogen receptors (ERs) was detected using real- time PCR, and ER silencing was performed. Results: At both the low- and high-doses, genistein significantly increased the bone mineral density (BMD) and bone volume, and resulted in thicker subchondral trabecular bone in vivo. In both in vivo and in vitro study, the low-dose genistein significantly increased the expression of ALP, OC and OPG, but decreased the expression of RANKL and the RANKL/OPG ratio. The high-dose genistein decreased the expression of all these bone homeostasis-associated factors. Both the low and high doses of genistein significantly increased the expression of ERβ, while ERα expression was increased by the low dose genistein and decreased by the high dose genistein. ERβ silencing abrogated most of the effects of genistein treatment. Conclusion: In rat mandibular condylar subchondral bone, low-dose genistein increases bone formation and inhibit bone resorption, while excess genistein inhibits both bone formation and resorption. The effects of genistein were predominantly mediated through ERβ. | Yong-qiLi Xiang-huiXing HuiWang Xi-liWeng Shi-binYu Guang-yingDong | 2012 | Acta Pharmacologica Sinica2012，33，1： | 21 |
| 3 | Capsule oxymatrine in treatment of hepatic fibrosis due to chronic viral hepatitis:A randomized,double blind,placebo-controlled,multicenter clinical study显示文摘AIM: To evaluate the efficacy and safety of oxymatrine capsule in treatment of hepatic fibrosis in patients with chronic viral hepatitis. METHODS: It was a randomized, double blind, placebocontrolled, multicenter clinical study. One hundred and fortyfour patients were divided into oxymatrine capsule group (group A) and placebo group (group B). The course was 52wk. Patients were visited once every 12wk and the last visit was at 12wk after cessation of the treatment. All patients had liver biopsy before treatment, part of them had a second biopsy at the end of therapy. Clinical symptoms, liver function test, serum markers of hepatic fibrosis were tested. Ultrasound evaluation was performed before, during and at the end of therapy. RESULTS: One hundred and forty-four patients enrolled in the study. Of them 132 patients completed the study according to the protocol, 49 patients had liver biopsy twice (25 patients in group A and 24 in group 13). At the end of therapy, significant improvements in hepatic fibrosis and inflammatory activity based on Semi-quantitative scoring system (SSS) were achieved in group A. The total effective rate of the treatment was 48.00%, much higher than that of 4.17% in group B (P<0.05). Significant improvement in serum markers of hepatic fibrosis such as hyaluronic acid (HA) and type Ⅲ procollagenic peptide (PⅢ P) in group A was seen (P<0.05). The total effective rate of serum markers at the end of therapy in group A was 68.19%, much higher than that of 34.85% in group B (P<0.05). The total effective rate of noninvasive markers at the end of therapy in group A was 66.67%, much higher than that of 30.30% in group B (P<0.05). The rate of adverse events was similar in two groups. CONCLUSION: Oxymatrine capsule is effective and safe in treatment of hepatic fibrosis due to chronic viral hepatitis. | Yi-MinMao Min-DeZeng Lun-GenLu Mo-BinWan Cheng-ZhongLi Cheng-WeiChen Qing-ChuenFu Ji-YaoWang Wei-MinShe XiongCai JunYe Xia-QiuZhou HuiWang Shan-MingWu Mei-FangTang Jin-ShuiZhu Wei-XiongChen Hui-QuanZhang | 2004 | World Journal of Gastroenterology2004，10，22： | 18 |
| 4 | Process analysis of PM_(2.5) pollution events in a coastal city of China using CMAQ显示文摘US EPA's Community Multiscale Air Quality modeling system(CMAQ) with Process Analysis tool was used to simulate and quantify the contribution of individual atmospheric processes to PM_(2.5) concentration in Qingdao during three representative PM_(2.5) pollution events in the winter of 2015 and 2016. Compared with the observed surface PM_(2.5) concentrations, CMAQ could reasonably reproduce the temporal and spatial variations of PM_(2.5) during these three events. Process analysis results show that primary emissions accounted for 72.7%–93.2% of the accumulation of surface PM_(2.5) before and after the events.When the events occurred, primary emissions were still the major contributor to the increase of PM_(2.5) in Qingdao, however the contribution percentage reduced significantly,which only account for 51.4%–71.8%. Net contribution from horizontal and vertical transport to the accumulation of PM_(2.5) was also positive and its percentage increased when events occurred. Only 1.1%–4.6% of aerosol accumulation was due to PM processes and aqueous chemical processes before and after events. When the events occurred,contribution from PM processes and aqueous chemistry increased to 6.0%–11.8%. Loss of PM_(2.5) was mainly through horizontal transport, vertical transport and dry deposition, no matter during or outside the events. Wet deposition would become the main removal pathway of PM_(2.5), when precipitation occurred. | Qiang Zhang Di Xue Xiaohuan Liu Xiang Gong Huiwang Gao | 2019 | Journal of Environmental Sciences2019，31，5： | 17 |
| 5 | Activation of nuclear factor-kappa B and effects of pyrrolidine dithiocarbamate on TNBS-induced rat colitis显示文摘AIM: To explore the changes of nuclear factor-kappa B (NF-κB) DNA-binding activity, the expression of intercellular adhesion molecule-1 (ICAM-1) regulated by NF-κB at various times and to evaluate the effects of pyrrolidine dithiocarbamate (PDTC) on trinitrobenzene sulfonic acid (TNBS)-induced rat colitis.METHODS: TNBS of 0.6 mL was mixed with ethanol of 0.3 mL solution and instilled into the lumen of the rat colon. The rat models were divided into 6 groups, which were killed at 24 h, 3, 7, 14, and 21 d after enema. Colonic inflammation and damage were assessed by macroscopical and histological criteria. Activity of NF-κB DNA-binding was analyzed by electrophoresis mobility shift assays (EMSA).Expression of ICAM-1 was detected by in situ hybridization (ISH) and immunohistochemistry (IH). Then various doses of PDTC were injected into rat abdomen 30 min before enema with TNBS/ethanol as pretreatment. The rats were killed 4 h after enema and the colonic inflammation,myeloperoxidase (MPO) activity, malondialdehyde (MDA)level, and DNA-binding activity of NF-κB were assessed.Finally, PDTC was injected intraperitoneally after colitis was induced. Changes of morphology were assayed.RESULTS: During the first week, hyperemia, hemorrhage,edema and ulceration of the colonic mucosa appeared with predominant infiltration of leukocytes. Neutrophils,macrophages, lymphocytes infiltrated in mucosa and submucosa 14 d later. Fibroblasts and granuloma-like structures were also obviously seen. The binding activity of NF-κB began to increase at 24 h time point and reached a peak at 14 d, then decreased but still was higher than control group at 21 d (P<0.01). Levels of tCAM-1 mRNA and protein significantly elevated at 24 h and the peak was at 21 d. Pretreatment with PDTC could attenuate the development of inflammation but not by reducing NF-κB activity. This attenuation of inflammation had a positive relationship with the dose of PDTC. PDTC at the dose of 100 mg/kg had no therapeutic effect after colitis was induced.CONCLUSION: NF-κB activation is an important event that may be involved in acute and chronic inflammation development and may contribute to self-protection against early inflammation damage. NF-κB also regulates ICAM-1expression during colonic inflammation. Pretreatment of PDTC may attenuate the inflammation development. But PDTC has no therapeutic effect after the colitis is induced. | KenChen You-MingLong HuiWang LeiLan Zhen-HeLin | 2005 | World Journal of Gastroenterology2005，11，10： | 13 |
| 6 | Sources and characteristics of fine particles over the Yellow Sea and Bohai Sea using online single particle aerosol mass spectrometer显示文摘Marine aerosols over the East China Seas are heavily polluted by continental sources. During the Chinese Comprehensive Ocean Experiment in November 2012, size and mass spectra of individual atmospheric particles in the size range from 0.2 to 2.0 μm were measured on board by a single particle aerosol mass spectrometer(SPAMS). The average hourly particle number(PN)was around 4560 ± 3240 in the South Yellow Sea(SYS), 2900 ± 3970 in the North Yellow Sea(NYS), and 1700 ± 2220 in the Bohai Sea(BS). PN in NYS and BS varied greatly over 3 orders of magnitude, while that in SYS varied slightly. The size distributions were fitted with two log-normal modes. Accumulation mode dominated in NYS and BS, especially during episodic periods. Coarse mode particles played an important role in SYS. Particles were classified using an adaptive resonance theory based neural network algorithm(ART-2a). Six particle types were identified with secondary-containing, aged sea-salt, soot-like, biomass burning, fresh sea-salt,and lead-containing particles accounting for 32%, 21%, 18%, 16%, 4%, and 3% of total PN,respectively. Aerosols in BS were relatively enriched in particles from anthropogenic sources compared to SYS, probably due to emissions from more developed upwind regions and indicating stronger influence of continental outflow on marine environment. Variation of source types depended mainly on origins of transported air masses. This study examined rapid changes in PN, size distribution and source types of fine particles in marine atmospheres. It also demonstrated the effectiveness of high-time-resolution source apportionment by ART-2a. | Huaiyu Fu Mei Zheng Caiqing Yan Xiaoying Li Huiwang Gao Xiaohong Yao Zhigang Guo Yuanhang Zhang | 2015 | Journal of Environmental Sciences2015，27，3： | 12 |
| 7 | Application of autologous tumor cell vaccine and NDV vaccine in treatment of tumors of digestive traet显示文摘AIM. TO treat patients with stage I-IV malignant tumors of digestive tract using autologous tumor cell vaccine and NDV(Newcastle disease virus) vaccine, and observe the survival period and curative effect.METHODS: 335 patients with malignant tumors of digestive tract were treated with autologous tumor cell vaccine and NDV vaccine. The autologous tumor cell vaccine received were assigned for long-term survival observation. While these failed to obtain the autologous tumor tissue were given with NDV vaccine for a received short-term observation on curative effect.RESULTS: The colorectal cancer patients treated with autologous tumor cell vaccine were divided into two groups:the controlled group (subjected to resection alone) (n=257),the vaccine group (subjected to both resection and immunotherapy) (n=310). 25 patients treated with NDV immunotherapy were all at stage IV without having resection.In postoperation adjuvant therapy patients, the 5, 6 and 7-year survival rates were 66.51%, 60.52 %, 56.50 %respectively; whereas in patients with resection alone, only 45.57 %, 44.76 % and 43.42 % respectively. The average survival period was 5.13 years (resection alone group 4.15 years), the median survival period was over 7 years (resection alone group 4.46 years). There were significant differences between the two groups. The patients treated with resection plus vaccine were measured delayed-type hypersensitivity (DTH) reactions after vaccination, (indurative scope >5 mm).The magnitude of DTH was related to the prognosis. The 5-year survival rate was 80 % for those with indurations greater than 5 mm, compared with 30 % for those with indurations less than 5 ram. The 1-year survival rate was 96 % for 25 patients treated with NDV immunotherapy. The total effective rate (CR+PR) was 24.00 % in NDV immunotherapy; complete remission (CR) in 1 case (4.00 %), partial remission (PR) in 5 cases (20.00 %), stabilizedin in 16 cases (64.00 %),progression (PD) in 1 case (4.00 %). After NDV vaccine immunotherapy, the number of NK cell increased and immune function imporved obviously.CONCLUSION: The autologous tumor cell vaccine and NDV vaccine can prolong the patients' life. NDV vaccine is notably effective for short-term with promotion of quality of life and can be used whenever necessary with good prospects. | WeiLiang HuiWang Tie-MieSun Wen-QingYao Li-LiChen YuJin Chun-LingLi Fan-JuanMeng | 2003 | World Journal of Gastroenterology2003，9，3： | 12 |
| 8 | Contrast-enhanced ultrasonographic imaging diagnosis on assessment of vascularity in liver metastatic lesions显示文摘AIM: To investigate the vasculature of rabbit liver metastatic lesions by color Doppler imaging and power Doppler imaging (PDI) techniques.METHODS: Eight New Zealand rabbits with implanted VX2 liver tumors were used. All ultrasound examinations were performed with a HP 5500 color Doppler ultrasound scanner. Before and after the injection of contrast agent, the changes of gray scale and the periphery and intralesional blood flow of the liver metastatic lesion were carefully observed by B mode ultrasound, color Doppler flow imaging (CDFI)and PDI.RESULTS: Twelve lesions were found in the eight rabbits with implanted VX2 liver tumors, whose diameter rangedfrom 1.6 to 4.8 cm. Echoes of these lesions were not characterized and has lack of specificity. After the injectionof contrast agent, the numbers of dot or strip-like flow messages increased both at the periphery and inside of these lesions under the mode of CDFI and PDI, and were more pronounced under PDI. Morphology of intralesional vessels extended, even branched and some signals were clearly found encircling the lesion. And some vessels were found penetrating into the center of the lesion.CONCLUSION: PDI after injection of self-made echo contrast agent can show a pronounced sensitivity than that of B mode ultrasound and CDFI in diagnosis of vascularity of a metastatic lesion. | Wen-HuaDu Wei-XiaoYang Xiu-QinXiong XiangWang YiZhou HuiWang | 2005 | World Journal of Gastroenterology2005，11，23： | 9 |
| 9 | Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer显示文摘The spatial distribution of ammonia-oxidizing Betaproteobacteria ( β AOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary.Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006.The abundance of β AOB ranged from 1.87 × 10 5 to 3.53 × 10 5 cells/g of sediment.β AOB abundance did not present a negative correlation with salinity,whereas salinity was implicated as the primary factor affecting nitrification rates.The DGGE profiles of PCR-amplified amo A gene fragments revealed that the β AOB community structure of sample S2 separated from other samples at the level of 40% similarity.The variations in composition of β AOB were significantly correlated with the salinity,temperature,absorption ability of sediments and TOC. The statistical analysis indicates that the β AOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity.Considering the good correlation between β AOB abundance and nitrification estimates,the abundance and diversity of β AOB community could be expected as an indirect index of nitrification activity at the study sea area in summer. | LI Jialin BAI Jie GAO Huiwang LIU Guangxing | 2011 | Acta Oceanologica Sinica2011，30，3： | 6 |
| 10 | Simulating Dry Deposition Fluxes of PM_(10) and Particulate Inorganic Nitrogen over the Eastern China Seas During a Severe Asian Dust Event Using WRF-Chem Model显示文摘A WRF-Chem model including a comprehensive gas-phase nitrogen chemistry module was used to simulate a severe dust event appearing in the eastern China on 19-25 March, 2002. The modeling result well reproduced PM10 concentrations in various distances from the dust sources and the transport pathway of the dust strom. The results showed that both the concentrations and the dry deposition fluxes of PM10 increased over the China seas during the dust event following the passage of a cold front system. The maximum fluxes of PM10 in the Yellow Sea and the East China Sea during the dust event were 5.5 and 8.4 times of those before the event, respectively. However, the temporal variations of the dry deposition fluxes of particulate inorganic nitrogen differed over the Yellow Sea from those over the East China Sea. Nitrate and ammonium in the whole northern China rapidly decreased because of the intrusion of dust-loaded air on 19 March. The dust plume arrived in the Yellow Sea on 20 March, decreasing the particulate inorganic nitrogen in mass concentration accordingly. The minimum dry deposition fluxes of nitrate and ammonium in the Yellow Sea were about 3/5 and 1/6 of those before the dust arrival, respectively. In contrast, when the dust plume crossed over the Yangtze Delta area, it became abundant in nitrate and ammonium and increased the concentrations and dry deposition fluxes of particulate inorganic nitrogen over the East China Sea, where the maximum dry deposition fluxes of nitrate and ammonium increased approximately by 4.1 and 2.6 times of those prior to the dust arrival. | YAN Han GAO Huiwang YAO Xiaohong WANG Zifa | 2012 | Journal of Ocean University of China2012，11，3： | 5 |
| 11 | The Effects of Reclamation Activity and Yellow River Runoff on Coastline and Area of the Laizhou Bay,China显示文摘Study on morphological changes of a bay can help to identify the effects of anthropogenic activities on coastal environ-ment and guide the exploration of marine resources.In this paper,morphological data including coastline and water areas in five discrete years between 1968 and 2015 were selected and extracted from the remote sensing images and historical marine charts to study the morphological changes in Laizhou Bay(LZB),one of the bays in the southwest of the Bohai Sea.A systematic analysis on spatial variations of the coastline and the surface areas of different types of waters in LZB was conducted.The results showed that the surface area of LZB was decreased by 1253.2km^(2)in the last half century,which is 17.4%of the total in the 1970s.The areas of the natural wetland and the intertidal zone were decreased by 17.2%and 56.1%,respectively,and the average water depth varied from 9.05 m to 8.16m at low tide level from 1968 to 2015.The coastline and shape variations of the bay turned to be complex after the 1980s,and the shape index of LZB showed an increasing trend in more recent years.The centroid of the bay generally migrated to the northeast direction,i.e.,the direction of the center of the Bohai Sea,and the shrinking direction of the bay was consistent with the migration direction of the coastline.The reclamation area during 1968-2015 in LZB was 1201.7km^(2),and 94.1%was in the inter-tidal zone.The overall morphological change of the bay during the last half century was mainly controlled by the coastal reclamation activities,and the Yellow River runoff including the river course change and sediment load variation was also an important control-ling factor. | XU Yandong GAO Huiwang WEI Xiao ZHU Jinlong | 2021 | Journal of Ocean University of China2021，20，3： | 4 |
| 12 | Distributions of Inorganic Nutrients in the Bohai Sea of China显示文摘To study the contents and distribution of inorganic nutrients in the Bohai Sea of China, two cruise surveys were undertaken in August (summer) 2000 and January (winter) 2001, respectively. A total of 595 water samples were collected from 91 stations and five nutrients, i.e., nitrate, nitrite, ammonia, phosphate and silicate, were analyzed for each sample. The results show that the average concentration of dissolved inorganic nitrogen (DIN) in the Bohai Sea in winter (6.529 μmol L -1) is significantly higher than that in summer (3.717 μmol L -1). The phosphorus concentration in winter (0.660 μmol L -1) is also significantly higher than that in summer (0.329 μmol L -1). Mean silicate concentration in winter (7.858 μmol L -1) is, however, not significantly different from that in summer (7.200 μmol L -1). Nutrients also vary considerably in different areas in Bohai Sea. DIN concentration in the Laizhou Bay (4.444 μmol L -1), for example, is significantly higher than those in the Bohai Bay (2.270 μmol L -1) and Bohai Strait (2.431 μmol L -1), which is caused by the discharge of large amounts of nitrogen into Laizhou Bay via Yellow River. The nutrients show different vertical distribution patterns. In summer, nutrients in bottom layer are generally richer than those in surface and middle layers. In winter, however, nutrients are not significantly different in different layers. Compared with historic data, DIN contents have increased continually since the early 1980 s. Based on atomic ratios of different nutrients, nitrogen is still the limiting factor for algal growth in the Bohai Sea. | LI Zhengyan *, BAI Jie, SHI Jinhui, GAO Huiwang College of Environmental Science and Engineering, Ocean University of China, Qingdao 266003, P..R.China | 2003 | Journal of Ocean University of Qingdao2003，2，1： | 4 |
| 13 | Size and elemental composition of dry-deposited particles during a severe dust storm at a coastal site of Eastern China显示文摘Dry-deposited particles were collected during the passage of an extremely strong dust storm in March, 2010 at a coastal site in Qingdao(36.15°N, 120.49°E), a city located in Eastern China. The size, morphology, and elemental composition of the particles were quantified with a scanning electron microscope equipped with an energy dispersive X-ray instrument(SEM–EDX). The particles appeared in various shapes, and their size mainly varied from 0.4to 10 μm, with the mean diameters of 0.5, 1.5, and 1.0 μm before, during, and after the dust storm, respectively. The critical size of the mineral particles settling on the surface in the current case was about 0.3–0.4 μm before the dust storm and about 0.5–0.7 μm during the dust storm. Particles that appeared in high concentration but were smaller than the critical size deposited onto the surface at a small number flux. The elements Al, Si and Mg were frequently detected in all samples, indicating the dominance of mineral particles. The frequency of Al in particles collected before the dust storm was significantly lower than for those collected during and after the dust storm. The frequencies of Cl and Fe did not show obvious changes, while those of S, K and Ca decreased after the dust arrival. These results indicate that the dust particles deposited onto the surface were less influenced by anthropogenic pollutants in terms of particle number. | Hongya Niu Daizhou Zhang Wei Hu Jinhui Shi Ruipeng Li Huiwang Gao Wei Pian Min Hu | 2016 | Journal of Environmental Sciences2016，28，2： | 4 |
| 14 | Alterations in microbial community during the remediation of a black-odorous stream by acclimated composite microorganisms显示文摘Microbial application is an efficient, economical, and ecofriendly method for remediating black-odorous rivers. In this study, the field treatment effect and microbial community changes were monitored during remediation by the acclimated complex microorganisms of a typical black-odorous stream. After the treatment, the total phosphorus and ammonia contents decreased by 74.0% and 76.3% and the concentrations of dissolved oxygen increased from 1.65 to 4.90 mg/L, indicating the effectiveness of the acclimated composite microorganisms. The proportion of Bacteroidetes decreased significantly by 48.1% and that of Firmicutes increased by 2.23% on average, and the microbial diversity index first increased and then tended to be uniform. Redundancy analysis demonstrated that the pH,dissolved oxygen, and oxidation-reduction potential together determined the composition of the microbial communities(p < 0.05). These findings showed that the acclimated composite microorganisms can effectively remediate the black odor. | Feng Shi Zongli Liu Jialin Li Huiwang Gao Song Qin Jingjing Guo | 2022 | Journal of Environmental Sciences2022，34，8： | 3 |
| 15 | A novel,rapid strategy to form dendritomas from human dendritic cells and hepatocellular carcinoma cell line HCCLM3 cells using mature dendritic cells derived from human peripheral blood CD14+monocytes within 48 hours of in vitro culture显示文摘AIM: Dendritomas formed by fusing cancer cells to dendritic cells have already been applied to clinical treatment trial of several types of cancers. Dendritic cells for the fusion in most trials and experiments were from blood monocytes in standard 7-d protocol culture, which requires 5-7 d of culture with granulocyte-macrophage-colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), followed by 2-3 d of activation with a combination of proinflammatory mediators such as tumor necrosis factorα (TNFα), interleukin-1β (IL-1β), interleukin-6 (IL-6) and prostaglandin E2 (PGE2).One study showed that mature monocyte-derived dendritic cells could be obtained within 48 h of in vitro culture with the same protocol as standard 7-d culture and referred to as FastDCs. Here we aimed to fuse human hepatocellular carcinoma cell line HCCLM3 cells with mature monocytederived dendritic cells within 48 h of in vitro culture (FastDC).METHODS: HCCLM3 cells were cultured in RPMI 1640 with 150 mL/L fetal calf serum (FCS). CD14+monocytes from healthy human peripheral blood were purified with MACS CD14 isolation kit and cultured in six-well plates in fresh complete DC medium containing RPMI-1640, 20 mL/L heat inactivated human AB serum, 2 mmol/1 L-glutamine,100 μg/mL gentamicin, 1000 U/mL GM-CSF and 500 U/mL IL-4 for 24 h, then proinflammatory mediators such as TNFα(1000 U/mL), IL-1β (10 ng/mL), IL-6 (10 ng/mL) and PGE2(1 μg/mL) were supplemented for another 24 h, and thus mature FastDCs were generated. HCCLM3 cells and FastDCs were labeled with red fluorescent dye PKH26-GL and green fluorescent dye PKH67-GL respectively. After the red fluorescent-stained HCCLM3 cells were irradiated with 50 Gy, FastDCs and irradiated HCCLM3 cells were fused in 500 mL/1 polyethylene glycol(PEG)+100 mL/L dimethyl sulfoxide (DMSO) to generate novel dendritornas. The FastDCs and novel dendritomas were immunostained with antiCD80, anti-CD86, anti-CD83, anti-HLA-DR mAbs and analyzed by fluorescence-activated cell sorting (FACS).Novel dendritomas were nucleus-stained with Hoechst 33258 and analyzed by confocal laser scanning microscopy.RESULTS: Mature FastDCs with highly expressed surface markers CD80, CD86, CD83 and HLA-DR were generated within 48 h in vitro. Novel dendritomas with dual red-green fluorescence were constructed fast and successfully, and FACS analysis showed that the fusion efficiency was 24.27% and the novel dendritomas expressed the same activation markers as FastDCs. Confocal laser scanning microscopy analysis showed representative images of dendritomas.CONCLUSION: Dendritomas can be formed fast with mature FastDCs from healthy human peripheral blood monocytes (PBMC) by incubation with GM-CSF and IL-4 for 24 h and by activation with proinflammatory mediators for an additional period of 24 h. Owing to shorter time required for in vitro DCs development, the generation of these novel dendritomas reduced labor and cost. This rapid method for formation of dendritomas may represent a new strategy for immunotherapy of hepatocellular carcinoma. | XinGuan Ji-RunPeng LanYuan HuiWang Yu-HuaWei Xi-ShengLeng | 2004 | World Journal of Gastroenterology2004，10，24： | 3 |
| 16 | Modeling Seasonal Variations of Subsurface Chlorophyll Maximum in South China Sea显示文摘In the South China Sea(SCS), the subsurface chlorophyll maximum(SCM) is frequently observed while the mechanisms of SCM occurrence have not been well understood. In this study, a 1-D physical-biochemical coupled model was used to study the seasonal variations of vertical profiles of chlorophyll-a(Chl-a) in the SCS. Three parameters(i.e., SCM layer(SCML) depth, thickness, and intensity) were defined to characterize the vertical distribution of Chl-a in SCML and were obtained by fitting the vertical profile of Chl-a in the subsurface layer using a Gaussian function. The seasonal variations of SCMs are reproduced reasonably well compared to the observations. The annual averages of SCML depth, thickness, and intensity are 75 ± 10 m, 31 ± 6.7 m, and 0.37 ± 0.11 mg m-3, respectively. A thick, close to surface SCML together with a higher intensity occurs during the northeastern monsoon. Both the SCML thickness and intensity are sensitive to the changes of surface wind speed in winter and summer, but the surface wind speed exerts a minor influence on the SCML depth; for example, double strengthening of the southwestern monsoon in summer can lead to the thickening of SCML by 46%, the intensity decreasing by 30%, and the shoaling by 6%. This is because part of nutrients are pumped from the upper nutricline to the surface mixed layer by strong vertical mixing. Increasing initial nutrient concentrations by two times will increase the intensity of SCML by over 80% in winter and spring. The sensitivity analysis indicates that light attenuation is critical to the three parameters of SCM. Decreasing background light attenuation by 20% extends the euphotic zone, makes SCML deeper(~20%) and thicker(12% – 41%), and increases the intensity by over 16%. Overall, the depth of SCML is mainly controlled by light attenuation, and the SCML thickness and intensity are closely associated with wind and initial nitrate concentration in the SCS. | GONG Xiang SHI Jie GAO Huiwang | 2014 | Journal of Ocean University of China2014，13，4： | 3 |
| 17 | Historic changes in flux of matter and nutrient budgets in the Bohai Sea显示文摘Over the past four periods (1959-1960,1982-1983,1992-1993,and 1998-1999),the ecosystem of the Bohai Sea changed due to both a significant decrease of river water discharge from the Huanghe River and a reduction of precipitation. The shifts in nutrient chemistry could result in changes in the phytoplankton composition with an increased potential for non-diatom algal blooms. Simple box model was used to estimate the water-mass balance and nutrient budgets for the Bohai Sea. Water budgets indicate that the residual flow changed from out of the Bohai Sea before 1993,but became inflow to the Bohai Sea after then. The nutrient budgets developed indicate that the Bohai Sea was a sink for nutrients except for phosphate in 1959-1960 and 1982-1983 and for silicate in 1982-1983. Net water flow transports nutrients out of the Bohai Sea in 1959-1960,1982-1983 and 1992-1993,but into the sea in 1998-1999 due to climate changes,such as precipitation and subsequent freshwater discharge. The residual fluxes of nutrients are minor relative to atmospheric deposition and riverine inputs. Conversions of phosphate values to carbon by stoichiometric ratios were used to predict that the system was net heterotrophic before 1982-1983 and net autotrophic after then. Nutrient budgets can explain the change of nutrient concentrations in the Bohai Sea except nitrates,which should include the surface runoff. | LIU Sumei ZHANG Jing GAO Huiwang LIU Zhe | 2008 | Acta Oceanologica Sinica2008，27，5： | 3 |
| 18 | Organizational Culture in China: An Analysis of Culture Dimensions and Culture Types显示文摘 | Anne S.Tsui HuiWang Katherine R.Xin | 2006 | Management and Organization Review2006，，3： | 3 |
| 19 | The vertical attenuation of irradiance as a function of turbidity: a case of the Huanghai (Yellow) Sea in spring显示文摘The planar photosynthetically available radiation (PAR), turbidity and concentration of chlorophyll a (chl a), were measured at 26 stations in the Huanghai (Yellow) Sea during a cruise of China SOLAS from 19 to 27 March 2005. Due to low chl a (<0.35 mg · m-3 ) in upper layers (above 5 m), suspended particulate matter became the major factor that influenced the turbidity in early spring. The calculated vertical diffuse attenuation coefficient of PAR, K PAR , varied with water depths with a maximum value in the upper 5 m layer. The mean K PAR in survey area was 0.277 ± 0.07 m-1 that is considerably higher than most of the other case 2 waters. Within the survey area, K PAR also showed distinct regional characteristics, corresponding to the distribution of turbidity. Based on measurements, the relationship between K PAR and turbidity as well as chl a was established. It was suggested that suspended particulate matter plays an important role in light attenuation in the Huanghai (Yellow) Sea in spring. | LIN Shaoying ZOU Tao GAO Huiwang GUO Xinyu | 2009 | Acta Oceanologica Sinica2009，28，5： | 2 |
| 20 | Seasonal and Spatial Variations of Macro Benthos in the Intertidal Mudflat of Southern Yellow River Delta,China in 2007/2008显示文摘In order to examine the seasonal and spatial distributions of benthic animals in the intertidal mudflat of the southern Yellow River Delta,field investigations were carried out in 2007 and 2008 and multiple methods were applied.Results showed that,the biomass of macro benthos ranged at 0.75-1151.00 g wet m^(-2) and averaged at 156.31 g wet m^(-2),in which Mactra veneriformis accounted for 75.6%-93.4% of the total macro benthic biomass.More than 90% of macro benthos inhabited in the middle and low tide lines,and higher biomass occurred in early summer and lower in winter.Statistical analysis showed that:1)M.veneriformis growth was primarily favored at higher temperature and lower salinity;2)after long time interaction,benthic bivalve grazers led to patching distributions of Chlorophyll a(Chl a);3)macro benthic biomass positively related with Chl a when the concentration of Chl a was low,but they were negatively related when Chl a concentration was high;and 4)furthermore,the biomass of benthic bivalves peaked in the sediment with median grain size about 0.55 mm,but decreased gradually in coarse or fine sediments.The secondary productivity ranged at 0.37-283.68 g m^(-2)yr^(-1) and averaged at 47.88 g m^(-2) yr^(-1),in which 69.7% was contributed by M.veneriformis It was estimated that primary production was transformed to secondary production at a rate of 6.87%approximately,which implies that there is a local sustainability of high bivalve production. | ZOU Li YAO Xiao YAMAGUCHI Hitomi GUO Xinyu GAO Huiwang WANG Kai SUN Mingyi | 2018 | Journal of Ocean University of China2018，17，2： | 2 |
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