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| 1 | PARylation regulates stress granule dynamics, phase separation, and neurotoxicity of disease-related RNA-binding proteins显示文摘Mutations in RNA-binding proteins (RBPs) localized in ribonucleoprotein (RNP) granules, such as hnRNP A1 and TDP-43, promote aberrant protein aggregation, which is a pathological hallmark of various neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Protein posttranslational modifications (PTMs) are known to 佗gulate RNP granules. In this study, we investigate the function of poly(ADP-ribosyl)ation (PARylation), an important PTM involved in DNA damage repair and cell death, in RNP granule-related neurodegeneration. We reveal that PARylation levels are a major regulator of the assembly-disassembly dynamics of RNP granules containing disease-related RBPs, hnRNP A1 and TDP-43. We find that hnRNP A1 can both be PARylated and bind to PARylated proteins or poly(ADP-ribose)(PAR). We further uncover that PARylation of hnRNP A1 at K298 controls its nucleocytoplasmic transport, whereas PAR-binding via the PAR-binding motif (PBM) of hnRNP Al regulates its association with stress granules. Moreover, we reveal that PAR not only dramatically enhances the liquid-liquid phase separation of hnRNP A1, but also promotes the co-phase separation of hnRNP A1 and TDP-43 in vitro and their interaction in vivo. Finally, both genetic and pharmacological inhibition of PARP mitigates hnRNP Al- and TDP-43-mediated neurotoxicity in cell and Drosophila models of ALS. Together, our findings suggest a novel and crucial role for PARylation in regulating the dynamics of RNP granules, and that dysregulation in PARylation and PAR levels may con tribute to ALS disease pathoge nesis by promoting protei n aggregation. | Yongjia Duan Aiying Du Jinge Gu Gang Duan Chen Wang Xinrui Gui Zhiwei Ma Beituo Qian Xue Deng Kai Zhang Le Sun Kuili Tian Yaoyang Zhang Hong Jiang Cong Liu Yanshan Fang | 2019 | Cell Research2019，29，3： | 13 |
| 2 | Activation of CaMKIIδA promotes Ca^2+ leak from the sarcoplasmic reticulum in cardiomyocytes of chronic heart failure rats显示文摘 | Le GUI Xin GUO Zhe ZHANG Hui XU Ya-wei JI Ren-jun WANG Jiang-hua ZHU Qing-hui CHEN | 2018 | Acta Pharmacologica Sinica2018，39，10： | 5 |
| 3 | Long-term adenosine A1 receptor activation-induced sortilin expression promotes α-synuclein upregulation in dopaminergic neurons显示文摘Prolonged activation of adenosine A1 receptor likely leads to damage of dopaminergic neurons and subsequent development of neurodegenerative diseases.However,the pathogenesis underlying long-term adenosine A1 receptor activation-induced neurodegeneration remains unclear.In this study,rats were intraperitoneally injected with 5 mg/kg of the adenosine A1 receptor agonist N6-cyclopentyladenosine(CPA)for five weeks.The mobility of rats was evaluated by forced swimming test,while their cognitive capabilities were evaluated by Y-maze test.Expression of sortilin,α-synuclein,p-JUN,and c-JUN proteins in the substantia nigra were detected by western blot analysis.In addition,immunofluorescence staining of sortilin andα-synuclein was performed to detect expression in the substantia nigra.The results showed that,compared with adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine(5 mg/kg)+CPA co-treated rats,motor and memory abilities were reduced,surface expression of sortin andα-synuclein in dopaminergic neurons was reduced,and total sortilin and totalα-synuclein were increased in CPA-treated rats.MN9D cells were incubated with 500 nM CPA alone or in combination with 10μM SP600125(JNK inhibitor)for 48 hours.Quantitative real-time polymerase chain reaction analysis of sortilin andα-synuclein mRNA levels in MN9D cells revealed upregulated sortilin expression in MN9D cells cultured with CPA alone,but the combination of CPA and SP600125 could inhibit this expression.Predictions made using Jasper,PROMO,and Alibaba online databases identified a highly conserved sequence in the sortilin promoter that was predicted to bind JUN in both humans and rodents.A luciferase reporter assay of sortilin promoter plasmid-transfected HEK293T cells confirmed this prediction.After sortilin expression was inhibited by sh-SORT1,expression of p-JUN and c-JUN was detected by western blot analysis.Long-term adenosine A1 receptor activation levels upregulatedα-synuclein expression at the post-transcriptional level by affecting sortilin expression.The online tool Raptor-X-Binding and Discovery Studio 4.5 prediction software predicted that sortilin can bind toα-synuclein.Co-immunoprecipitation revealed an interaction between sortilin andα-synuclein in MN9D cells.Our findings indicate that suppression of prolonged adenosine A1 receptor activation potently inhibited sortilin expression andα-synuclein accumulation,and dramatically improved host cognition and kineticism.This study was approved by the University Committee of Animal Care and Supply at the University of Saskatchewan(approval No.AUP#20070090)in March 2007 and the Animals Ethics Committee of University of South China(approval No.LL0387-USC)in June 2017. | Yun-Cheng Lv An-Bo Gao Jing Yang Li-Yuan Zhong Bo Jia Shu-Hui Ouyang Le Gui Tian-Hong Peng Sha Sun Francisco S.Cayabyab | 2020 | Neural Regeneration Research2020，15，4： | 4 |
| 4 | In situ synthesis and hardness of TiC/Ti_5Si_3 composites on Ti-5Al-2.5Sn substrates by gas tungsten arc welding显示文摘TiC/Ti5Si3 composites were fabricated on Ti-5Al-2.5Sn substrates by gas tungsten arc welding (GTAW). Identification of the phases was performed using X-ray diffraction (XRD). The microstructures were analyzed using scanning electron microscopy (SEM) combined with energy-dispersive X-ray spectrometry (EDS) and optical microscopy (OM). The Vickers hardness was measured with a micro-hardness tester. The TiC/Ti5Si3 composites were obtained in a double-layer track, and the Vickers hardness of the track increased by two to three times compared with the Ti-5Al-2.5 Sn substrate. | Wen-qing Yan Le Dai Chi-bin Gui | 2013 | International Journal of Minerals,Metallurgy and Materials2013，20，3： | 3 |
| 5 | Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda显示文摘The fall armyworm(FAW),Spodoptera frugiperda,is a destructive pest native to America and has recently become an invasive insect pest in China.Because of its rapid spread and great risks in China,understanding of FAW genetic background and pesticide resistance is urgent and essential to develop effective management strategies.Here,we assembled a chromosome-level genome of a male FAW(SFynMstLFR)and compared re-sequencing results of the populations from America,Africa,and China.Strain identification of 163 individuals collected from America,Africa and China showed that both C and R strains were found in the American pop-ulations,while only C strain was found in the Chinese and African populations.Moreover,population geno-mics analysis showed that populations from Africa and China have close relationship with significantly genetic differentiation from American populations.Taken toge-ther,FAWs invaded into China were most likely origi-nated from Africa.Comparative genomics analysis displayed that the cytochrome p450 gene family is extremely expanded to 425 members in FAW,of which 283 genes are specific to FAW.Treatments of Chinese populations with twenty-three pesticides showed the variant patterns of transcriptome profiles,and several detoxification genes such as AOX,UGT and GST spe-cially responded to the pesticides.These findings will be useful in developing effective strategies for manage-ment of FAW in China and other invaded areas. | Furong Gui Tianming Lan Yue Zhao Wei Guo Yang Dong Dongming Fang Huan Liu Haimeng Li Hongli Wang Ruoshi Hao Xiaofang Cheng Yahong Li Pengcheng Yang Sunil Kumar Sahu Yaping Chen Le Cheng Shuqi He Ping Liu Guangyi Fan Haorong Lu Guohai Hu Wei Dong Bin Chen Yuan Jiang Yongwei Zhang Hanhong Xu Fei Lin Bernard Slippers Alisa Postma Matthew Jackson Birhan Addisie Abate Kassahun Tesfaye Aschalew Lemma Demie Meseret Destaw Bayeleygne Dawit Tesfaye Degefu Feng Chen Paul K.Kuria Zachary M.Kinyua Tong-Xian Liu Huanming Yang Fangneng Huang Xin Liu Jun Sheng Le Kang | 2022 | Protein & Cell2022，13，7： | 2 |
| 6 | Effects of chemical forms of alkali and alkaline earth metallic species on the char ignition temperature of a Loy Yang coal under O2/N2 atmosphere显示文摘 | Wu Le Qiao Yu Gui Ben | | 0，，01： | 1 |
| 7 | QUENCHING AND AGEING BEHAVIORS OF Mg-Li-Zn ALLOY显示文摘QUENCHINGANDAGEINGBEHAVIORSOFMgLiZnALLOY①LeQichi,CuiJianzhong,LiuHanwuDepartmentofMetalForming,ScholofMaterialandMetalurgy,... | Le, Qichi Gui, Jianzhong Liu, Hanwu | 1997 | 中国有色金属学会会刊:英文版1997，7，3： | 1 |
| 8 | Study on instability fracture and simulation of surrounding rock induced by fault activation under mining influence显示文摘The fault is potentially vulnerability's geological structure in the working face and its vicinity,and it is also a crucial geological factor affecting coal mine safety exploitation.To investigate the unstable failure of surrounding rock induced by fault activation under the influence of adoption,which was studied utilizing field case and numerical analysis for the deformation and failure process of surrounding rock near the fault-affected zone.Combined with field cases,this paper analyzes disturbance stress and roof abscission layer monitoring in effecting zones of fault activation.Using the discrete element 3DEC numerical analysis method,the model of surrounding rock unstable fracture induced by fault activation under adoption is established.The unstable fracture and stress variation characteristics of surrounding rock induced by fault activation during the excavation of the upper side wall and lower side wall of the faults are simulated and analyzed.Field analysis shows that as the coal working face continues to advance,the mining stress gradually increases.There is a zigzag wave on the relationship curve between coal mining and roof displacement near the fault,which reveals that the surrounding rock of the fault activation affected zone is in the superposition state of static load and dynamic load.Furthermore,the simulation results show that the stress and displacement of surrounding rock near the fault increase with the advance of coal mining face.The closer to the fault plane,the displacement gradually returns to zero,and the stress is also in a lower state. | Jie Chen Kaiwen Shi Yuanyuan Pu Derek B.Apel Chuanjiu Zhang Yujun Zuo Jiongkun Chen Le Xu Zhen Gui Longfei Song | 2023 | Rock Mechanics Bulletin2023，2，2： | 1 |
| 9 | Enhanced store-operated calcium entry in platelets is associated with acute coronary syndrome显示文摘Acute coronary syndrome(ACS)is assciated with sudden,reduced blood flow to the heart and usually correlated with coronary thrombosis[1].Therefore,platelets play an important part in the pathogenesis and complications of ACS.Plateler activarion is a complex event involving activation of intracellular signaling cascades,such as calcium signaling,which le ads to platelet actiration,secrerion and aggregation,Platelets are highly specialized secretory cells that have a well characterized pattern of cell activation and intracellular signaling. | Xin Guo Jiayu Zhang Jianhua Zhu Qing-Hui Chen Renjun Wang Le Gui | 2020 | Acta Biochimica et Biophysica Sinica2020，52，2： | 0 |
| 10 | A multicenter randomized study on Me-CCNU,5-FU and ADM versus ACNU,5-FU and ADM intreatment of advanced gastric cancer显示文摘AmulticenterrandomizedstudyonMeCCNU,5FUandADMversusACNU,5FUandADMintreatmentofadvancedgastriccancerXIAOShuDong1,LIDeHu... | XIAO ShuDong1, LI DeHua1, ZHANG DeZhong1, SHEN MouJi1, ZHU XiaoTing1, HE GuiFen2, ZHAO TiPing2, XI LePing2, DENG XingCun3, WANG Min3, WANG XiuLing4, CHEN Qiang4, ZHANG YongPing4, YAO CuiLing5, BAO Ji | 1997 | World Journal of Gastroenterology1997，3，4： | 0 |
| 11 | Study on the green extraction of corncob xylan by deep eutectic solvent显示文摘Corn as one of the world's major food crops,its by-product corn cob is also rich in resources.However,the unreasonable utilization of corn cob often causes the environmental pollution,waste of resources and other problems.As one of the most abundant polymers in nature,xylan is widely used in food,medicine,materials and other fields.Corn cob is rich in xylan,which is an ideal raw material for extracting xylan.However,the intractable lignin is covalently linked to xylan,which increases the difficulty of xylan extraction.It has been reported that the deep eutectic solvent(DES)could preferentially dissolve lignin in biomass,thereby dissolving the xylan.Then,the xylan in the extract was separated by ethanol precipitation method.The xylan precipitate was obtained after centrifugation,while the supernatant was retained.The components of the supernatant after ethanol precipitation were separated by the rotary evaporator.The ethanol,water and DES were collected for the subsequent extraction of corn cob xylan.In this study,a novel way was provided for the green production of corn cob xylan.The DES was used to extract xylan from corn cob which was used as the raw material.The effects of solid-liquid ratio,reaction time,reaction temperature and water content of DES on the extraction rate of corn cob xylan were investigated by the single factor test.Furthermore,the orthogonal test was designed to optimize the xylan extraction process.The structure of corn cob xylan was analyzed and verified.The results showed that the optimum extraction conditions of corn cob xylan were as follows:the ratio of corn cob to DES was 1:15(g:mL),the extraction time was 3 h,the extraction temperature was 60℃,and the water content of DES was 70%.Under these conditions,the extraction rate of xylan was 16.46%.The extracted corn cob xylan was distinctive triple helix of polysaccharide,which was similar to the structure of commercially available xylan.Xylan was effectively and workably extracted from corn cob by the DES method.This study provided a new approach for high value conversion of corn cob and the clean production of xylan. | Bingyu Jiao Le Wang Haitao Gui Zifu Ni Rong Du Yuansen Hu | 2024 | Grain & Oil Science and Technology2024，7，1： | 0 |
| 12 | Recombinant human thrombopoietin prior to mobilization chemotherapy facilitates platelet recovery in autologous transplantation in patients with lymphoma:Results of a prospective randomized study显示文摘Background:Chemotherapy plus granulocyte colony-stimulating factor (GCSF) regimen is one of the available approaches to mobilize peripheral blood progenitor cells (PBPCs).It causes thrombocytopenia and delays leukapheresis.This study aimed to evaluate the role of recombinant human thrombopoietin (rhTPO) before mobilization chemotherapy in facilitating leukapheresis in patients with lymphoma.Methods:In this randomized open-label phase 2 trial, patients were randomly assigned in a 1:2 ratio to receive mobilization with rhTPO plus GCSF in combination with chemotherapy (the rhTPO plus GCSF arm) or GCSF alone in combination with chemotherapy (the GCSF alone arm).The recovery of neutrophils and platelets and the amount of platelet transfusion were monitored.Results:Thirty patients were enrolled in this study between March 2016 and August 2018. Patients in the rhTPO plus GCSF arm (n = 10) had similar platelet nadir after mobilization chemotherapy (P=0.878) and similar amount of platelet transfusion (median 0 vs.1 unit,P=0.735) when compared with the GCSF alone arm (n = 20). On the day of leukapheresis, the median platelet count was 86 ×10^(9)/L (range 18-219) among patients who received rhTPO and 73 ×10^(9)/L (range 42-197) among those who received GCSF alone (P=0.982). After the use of rhTPO, the incidence of platelet count <75 ×10^(9)/L on the day of leukapheresis did not decrease significantly (30.0% vs. 50.0%,P=0.297).Platelet recovery after PBPC transfusion was more rapid in the rhTPO plus GCSF arm (median 8.0 days [95% confidence interval 2.9-13.1] to platelets ≥50 ×10^(9)/L vs. 11.0 days [95% confidence interval 8.6-13.4],P=0.011).The estimated total cost of the mobilization and reconstitution phases per patient was similar between the two treatmtent groups (P=0.362 andP=0.067,respectively).Conclusions:Our findings indicate that there was no significant clinical benefit of rhTPO use in facilitating mobilization of progenitor cells,but it may promote platelet recovery in the reconstitution phase after high-dose therapy.Trial registration:This trial has been registered in Clinicaltrials.gov as NCT03014102. | Hongnan Mo Peng Liu Yan Qin Xiaohui He Xiaohong Han Jiarui Yao Weicai Su Shuxiang Zhang Le Tang Fengyi Zhao Lin Gui Sheng Yang Jianliang Yang Shengyu Zhou Zhishang Zhang Yuankai Shi | 2021 | Chronic Diseases and Translational Medicine2021，7，3： | 0 |
| 13 | Sulfation modification of dopamine in brain regulates aggregative behavior of animals显示文摘Behavioral plasticity and the underlying neuronal plasticity represent a fundamental capacity of animals to cope with environmental stimuli.Behavioral plasticity is controlled by complex molecular networks that act under different layers of regulation.While various molecules have been found to be involved in the regulation of plastic behaviors across species,less is known about how organisms orchestrate the activity of these molecules as part of a coherent behavioral response to varying environments.Here we discover a me chanism for the regulation of animal behavioral plasticity involving molecular sulfation in the brain,a modification of substrate molecules by sulfo transferase(ST)-catalyzed addition of a sulfonate group(SO_(3))from an obligate donor,3'-phosphoadenosine 5'-phosphosulfate(PAPS)to the substrates.We investigated aggregation behaviors of migratory locusts,which are well-known for extreme phase change plasticity triggered by population density.The processes of PAPS biosynthesis acted efficiently on induction of locust behavioral transition:Inhibition of PAPS synthesis solicited a behavioral shift from gregarious to solitarious states;external PAPS dosage,by contrast,promoted aggregation in solitarious locusts.Genetic or pharmacological intervention in the sulfation catalyzation resulted into pronounced solitarizing effects.Analysis of substrate-specific STs suggests a widespread involvement of sulfated neurotransmitters in the behavioral response.D opamine in the brain was finally identified to be actively sulfate conjugate d,and the sulfate conjugation enhanced the free DA-mediated behavioral aggregation.Similar results in Caenorhabditis elegans and mice indicate that sulfation may be involved more broadly in the modulation of animal aggregation.These findings reveal a general me chanism that effectively regulates animal social-like behavioral plasticity,possibly through sulfation-mediated modification of neural networks. | Bing Chen Xiwen Tong Xia Zhang Wanying Gui Guoming Ai Lihua Huang Ding Ding Jiangxu Zhang Le Kang | 2022 | National Science Review2022，9，4： | 0 |
| 14 | Folate and Vitamin B_(12)Status and Related Dietary Factors among the Elderly Northern and Southern Rural Chinese Population显示文摘Folate and vitamin B_(12)are involved in the carbon metabolism pathway supplying essential methyl groups for DNA and protein synthesis and play a vital role in individual health.They have also been shown to be associated with cardiovascular disease,especially stroke.A recent study showed that stroke was the leading cause of death and disabilityadjusted life years(DALYs)at the national level in China.Therefore,the nutritional status of folate and vitamin B_(12)in the Chinese population is a concern.The elderly are more prone to develop folate and vitamin B_(12)deficiencies due to poor appetite,dental defects,and issues with digestion. | LIU Zhen MAN Qing Qing JIA Shan Shan SONG Peng Kun WANG Jing Zhong WANG Xi Le KANG Bing Xian YANG Li Gui ZHANG Jian | 2021 | Biomedical and Environmental Sciences2021，34，7： | 0 |
| 15 | In situ growth of large-area and self-aligned graphene nanoribbon arrays on liquid metal显示文摘Intrinsic graphene features semi-metallic characteristics that limit its applications in electronic devices,whereas graphene nanoribbons(GNRs) are promising semiconductors because of their bandgap-opening feature. However, the controllable mass-fabrication of high-quality GNR arrays remains a major challenge.In particular, the in situ growth of GNR arrays through template-free chemical vapor deposition(CVD) has not been realized. Herein, we report a template-free CVD strategy to grow large-area, high-quality and self-aligned GNR arrays on liquid copper surface. The width of as-grown GNR could be optimized to sub-10 nm with aspect ratio up to 387, which is higher than those of reported CVD-GNRs. The study of the growth mechanism indicates that a unique comb-like etching-regulated growth process caused by a trace hydrogen flow guides the formation of the mass-produced self-aligned GNR arrays. Our approach is operationally simple and efficient, offering an assurance for the use of GNR arrays in integrated circuits. | Le Cai Wanzhen He Xudong Xue Jianyao Huang Ke Zhou Xiahong Zhou Zhiping Xu Gui Yu | 2021 | National Science Review2021，8，12： | 0 |
| 16 | Aliskiren ameliorates sympathetic nerve sprouting and suppresses the inducibility of ventricular tachyarrhythmia in postinfarcted rat heart显示文摘 | JIA Yin-yu BAO Zhi-wei WEI Mei-fang ZHU Jian-hua GUI Le | 2013 | Chinese Medical Journal2013，，24： | 0 |
| 17 | Mutation analysis of KCNQ1, KCNH2, SCN5A, KCNE1 and KCNE2 genes in Chinese patients with long QT syndrome显示文摘Long QT syndrome(LQTS)is the prototype of the cardiac ion channelopathies,which cause syncope and sudden death.Inherited LQTS is represented by the autosomal dominant Romano-ward syndrome(RWS),which is not accompanied by congenital deafness,and the autosomal recessive Jervell and Lange-Nielsen syndrome(JLNS),which is accompanied by congenital deafness.The LQTS-causing mutations have been reported in patients and families from Europe,North America and Japan.Few genetic studies have been carried out in families with JLNS from China.This study investigates the molecular pathology in four families with LQTS(including a family with JLNS)in the Chinese population.Polymerase chain reaction and DNA sequencing were used to screen for KCNQ1,KCNH2,KCNE1,KCNE2 and SCN5A mutation.A missense mutation G314S in an RWS family was identified,and a single nucleotide polymorphism(SNP)G643S was indentified in the KCNQ1 of the JLNS family.In this JLNS family,another heterozygous novel mutation in exon 2a was found in KCNQ1 of the patients.Our data provide useful information for the identification of polymorphisms and mutations related to LQTS and the Brugada Syndrome(BS)in Chinese populations. | DU Rong TIAN Li YUAN Guohui LI Jin REN Faxin GUI Le LI Wei ZHANG Shouyan KANG Cailian YANG Junguo | 2007 | Frontiers of Medicine2007，1，3： | 0 |
| 18 | Standardized quantitative measurements of wrist cartilage in healthy humans using 3T magnetic resonance imaging显示文摘AIM: To quantify the wrist cartilage cross-sectional area in humans from a 3D magnetic resonance imaging(MRI) dataset and to assess the corresponding reproducibility. METHODS: The study was conducted in 14 healthy volunteers(6 females and 8 males) between 30 and 58 years old and devoid of articular pain. Subjectswere asked to lie down in the supine position with the right hand positioned above the pelvic region on top of a home-built rigid platform attached to the scanner bed. The wrist was wrapped with a flexible surface coil. MRI investigations were performed at 3T(Verio-Siemens) using volume interpolated breath hold examination(VIBE) and dual echo steady state(DESS) MRI sequences. Cartilage cross sectional area(CSA) was measured on a slice of interest selected from a 3D dataset of the entire carpus and metacarpalphalangeal areas on the basis of anatomical criteria using conventional image processing radiology software. Cartilage cross-sectional areas between opposite bones in the carpal region were manually selected and quantified using a thresholding method.RESULTS: Cartilage CSA measurements performed on a selected predefined slice were 292.4 ± 39 mm2 using the VIBE sequence and slightly lower, 270.4 ± 50.6 mm2, with the DESS sequence. The inter(14.1%) and intra(2.4%) subject variability was similar for both MRI methods. The coefficients of variation computed for the repeated measurements were also comparable for the VIBE(2.4%) and the DESS(4.8%) sequences. The carpus length averaged over the group was 37.5 ± 2.8 mm with a 7.45% between-subjects coefficient of variation. Of note, wrist cartilage CSA measured with either the VIBE or the DESS sequences was linearly related to the carpal bone length. The variability between subjects was significantly reduced to 8.4% when the CSA was normalized with respect to the carpal bone length.CONCLUSION: The ratio between wrist cartilage CSA and carpal bone length is a highly reproducible standardized measurement which normalizes the natural diversity between individuals. | Jean-Vincent Zink Philippe Souteyrand Sandrine Guis Christophe Chagnaud Yann Le Fur Daniela Militianu Jean-Pierre Mattei Michael Rozenbaum Itzhak Rosner Maxime Guye Monique Bernard David Bendahan | 2015 | World Journal of Orthopedics2015，6，8： | 0 |
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