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| 1 | Evaluation of three tracers for labeling distal cerebrospinal fluidcontacting neurons显示文摘It has been reported that distal cerebrospinal fluidcontacting neurons(dCSF-CNs)can be detected by immunohistochemical assay using cholera toxin subunit B-conjugated horseradish peroxidase(CBHRP).In the present study,another two methods with CB alone or CB-conjugated FITC(CB-FITC)were used,and the results from the three methods were compared.Adult Sprague-Dawley rats were randomly divided into three groups with CB-HRP,CB or CB-FITC.Tracers were diluted to 30%in artificial cerebrospinal fluid and injected separately(in a volume of 3μL)into the lateral ventricle.Animals from the CB-HRP and CB groups were perfused 48 h after surgery while animals from the CB-FITC group were perfused 1,3,6,12,24 or 48 h after surgery.The brain was sectioned(40μm)for immunofluorescence and five sections with positive neurons were selected from each rat for neuron counting.Three clusters of positive neurons in a'Y-like'distribution were detected ventral to the cerebral aqueduct of rats from the three groups.No significant difference was observed among the quantitative data.In the CB-FITC group,stable staining was detected even at 6 h after injection.Taken together,lateral ventricle injection of CB/CB-FITC is a useful method for labeling dCSFCNs in rats.The CB-FITC method makes dCSF-CNs labeling much simpler and more convenient. | Fang Zhou Jiayou Wang Hongxing Zhang He Liu Guangping Zhao Cuihua Zu Xiaoxing Lu Licai Zhang | 2013 | Neuroscience Bulletin2013，29，5： | 7 |
| 2 | The Use of Postoperative Serum HS-AFP and GGTⅡfor Judgment of the Prognosis for Hepatocellular Carcinoma patients显示文摘OBJECTIVE To investigate the clinical value of hepatoma-specific alpha -fetoprotein (HS-AFP) and gamma-glutamyltransferaseⅡ(GGTⅡ) for judgment of postoperative prognosis of patients with hepatocellular carcinoma (HCC). METHODS HS-AFP was separated and determined using native polyacrylamide electrophoresis with a discontinuous buffer system and Western blots. GGTⅡwas separated with native polyacrylamide electrophoresis with a discontinuous buffer system and detected by enzyme staining. Forty cases with HCC underwent serial determination of HS-AFP and GGTⅡbefore and after radical excision. The correlations were analyzed between the two indices and survival time. RESULTS In the 40 cases with HCC, before radical excision the positive rates of HS-AFP and GGTⅡwere 57.5% and 67.5% respectively, with the positive rate of combined HS-AFP and GGTⅡreaching 80.0%. After operation, the recurrence and metastasis rate in the groups with positive HS-AFP and GGTⅡwere 90.9% and 58.8% respectively, while in the groups with negative HS-AFP and GGTⅡthe rates were 20.7% and 26.1% respectively. Recurrence and metastasis occurred in all cases with both postoperative positive HS-AFP and GGTⅡbut only in 9.5% of the cases in whom both postoperative HS-AFP and GGTⅡwere negative. Univariate analysis revealed that postoperative HS-AFP and GGTⅡwere related to the prognosis in HCC. CONCLUSION Postoperative serum HS-AFP and GGTⅡare very useful in predicting the prognosis of HCC patients. | Runzhou Ni Lei Yang Mingbing Xiao Feng Li Cuihua Lu | 2006 | Chinese Journal of Clinical Oncology2006，3，4： | 1 |
| 3 | Upregulated expression of ubiquitin-conjugating enzyme E2Q1 (UBE2Q1) is associated with enhanced cell proliferation and poor prognosis in human hapatocellular carcinoma显示文摘 | Renan Chang Lixian Wei Yuhua Lu Xiaopeng Cui Cuihua Lu Luoliang Liu Dawei Jiang YiCheng Xiong Gang Wang Chunhua Wan Haixin Qian | 2015 | Journal of Molecular Histology2015，，1： | 1 |
| 4 | Composite Membrane of Sulfonated Poly (phenylene oxide) Doped with Phosphosilicate Gels for Direct Methanol Fuel Cell显示文摘 | Deping Lu Wei Lu Cuihua Li Jianhong Liu Rong Guan | 2007 | Polymer Bulletin2007，，4： | 1 |
| 5 | Step adap- tive normalization blind source separation algorithm显示文摘 | YIN HONGWEI LI GUOLIN LU CUIHUA | 2014 | Modern Technologies in Materials Mechanics and Intelli- gent Systems2014，51，3： | 1 |
| 6 | Step adap- tive normalization blind source separation algorithm显示文摘 | YIN HONGWEI LI GUOLIN LU CUIHUA | 2014 | Modem Technologies in Materials Mechanics and Intelli- gent Systems2014，85，2： | 1 |
| 7 | Decreased expression of Small glutamine-rich tetratricopeptide repeat- containing protein (SGT) correlated with prognosis of Hepatocellular carcinoma 显示文摘 | ZHANG Jing LU Cuihua HE Song | 2014 | Neoplasma2014，61，1： | 1 |
| 8 | Caching scheme based on power-law characteristic in unstructured P2P networks显示文摘 | Zuo Cuihua Li Ruixuan Lu Zhengding | 2009 | Journal of Southwest Jiaotong University2009，17，3： | 1 |
| 9 | Genetic Architecture of Childhood Kidney and Urological Diseases in China显示文摘Kidney disease is manifested in a wide variety of phenotypes,many of which have an important hereditary component.To delineate the genotypic and phenotypic spectrum of pediatric nephropathy,a multicenter registration system is being imple-mented based on the Chinese Children Genetic Kidney Disease Database(CCGKDD).In this study,all the patients with kidney and urological diseases were recruited from 2014 to 2020.Genetic analysis was conducted using exome sequencing for families with multiple affected individuals with nephropathy or clinical suspicion of a genetic kidney disease owing to early-onset or extrarenal features.The genetic diagnosis was confirmed in 883 of 2256(39.1%)patients from 23 provinces in China.Phenotypic profiles showed that the primary diagnosis included steroid-resistant nephrotic syndrome(SRNS,23.5%),glomerulonephritis(GN,32.2%),congenital anomalies of the kidney and urinary tract(CAKUT,21.2%),cystic renal disease(3.9%),renal calcinosis/stone(3.6%),tubulopathy(9.7%),and chronic kidney disease of unknown etiology(CKDu,5.8%).The pathogenic variants of 105 monogenetic disorders were identified.Ten distinct genomic disorders were identified as pathogenic copy number variants(CNVs)in 11 patients.The diagnostic yield differed by subgroups,and was highest in those with cystic renal disease(66.3%),followed by tubulopathy(58.4%),GN(57.7%),CKDu(43.5%),SRNS(29.2%),renal calcinosis/stone(29.3%)and CAKUT(8.6%).Reverse phenotyping permitted correct identification in 40 cases with clinical reassessment and unexpected genetic conditions.We present the results of the largest cohort of children with kidney disease in China where diagnostic exome sequencing was performed.Our data demonstrate the utility of family-based exome sequencing,and indicate that the combined analysis of genotype and phenotype based on the national patient registry is pivotal to the genetic diagnosis of kidney disease. | Ye Fang Hua Shi Tianchao Xiang Jiaojiao Liu Jialu Liu Xiaoshan Tang Xiaoyan Fang Jing Chen Yihui Zhai Qian Shen Guomin Li Li Sun Yunli Bi Xiang Wang Yanyan Qian Bingbing Wu Huijun Wang Wenhao Zhou Duan Ma Jianhua Mao Xiaoyun Jiang Shuzhen Sun Ying Shen Xiaorong Liu Aihua Zhang Xiaowen Wang Wenyan Huang Qiu Li Mo Wang Xiaojie Gao Yubin Wu Fang Deng Ruifeng Zhang Cuihua Liu Li Yu Jieqiu Zhuang Qing Sun Xiqiang Dang Haitao Bai Ying Zhu Siguang Lu Bili Zhang Xiaoshan Shao Xuemei Liu Mei Han Lijun Zhao Yuling Liu Jian Gao Ying Bao Dongfeng Zhang Qingshan Ma Liping Zhao Zhengkun Xia Biao Lu Yulong Wang Mengzhun Zhao Jianjiang Zhang Shan Jian Guohua He Huifeng Zhang Bo Zhao Xiaohua LI Feiyan Wang Yufeng Li Hongtao Zhu Xinhui Luo Jinghai Li Jia Rao Hong Xu | 2021 | Phenomics2021，1，3： | 0 |
| 10 | Clinical Value of Hepatoma-Specific Alpha-Fetoprotein in the Diagnosis of Hepatocellular Carcinoma显示文摘OBJECTIVE To study the clinical value of hepatoma-specific alpha-feto- protein (HS-AFP) in the diagnosis and differential diagnosis of hepato- cellular carcinoma (HCC). METHODS A method of vertical slab polyacrylamide gel electrophoresis with discontinuous buffer system was developed to separate AFP sub- types. After separation, the AFP subtypes were transferred to nitrocellu- lose and reacted first with rabbit anti-human AFP and then with goat an- ti-rabbit IgG -HRP. Finally, AFP subtypes were visualized by reacting with 3,3’-diaminobenzidine tetrahydrochloride. A HS-AFP band was de- termined in 82 cases with HCC and 95 cases with benign liver diseases. The correlations between the positive rates of HS-AFP and serum AFP concentration, tumor size as well as portal vein metastasis were analyzed. RESULTS Serum AFP in the cases with various liver diseases was sepa- rated into one to several bands. The fastest band on electrophoresis (F- AFP) was found in all patients, while the band at the cathodal site (HS- AFP) was detected predominantly in HCC but rarely in benign liver dis- eases. The positive rate of HS-AFP in HCC was 74.4%, which was sig- nificantly higher than that in benign liver diseases (9.1%, 7.3% and 10.0% in liver cirrhosis, chronic hepatitis and acute hepatitis respectively). HS- AFP was detected in 3 out of 9 HCC cases with AFP<50 μg/L , but in none of 22 cases of benign liver diseases with the same AFP concentra- tion. HS-AFP correlated with serum AFP concentration and tumor size to some extent, but not with portal vein metastasis. CONCLUSION HS-AFP increases the sensitivity of diagnosing HCC in patients with negative AFP, and is useful in distinguishing high AFP due to HCC from that caused by benign liver diseases. | Runzhou Ni Mingbing Xiao Fei Jin Cuihua Lu Jiefei Huang Xianyong Meng | 2006 | Chinese Journal of Clinical Oncology2006，3，3： | 0 |
| 11 | Development and Evaluation of Codon-modified cryV Constructs in Cultivated Potato(Solanum tuberosum L.) for Control of Potato Tuber Moth显示文摘A cryV gene,specifically toxic to Lepidoptera and Coleoptera,was incorporated into binary vectors with different promoters and the presence or absence of the β-glucuronidase(gus) reporter gene.These constructs were integrated into potato cv.Spunta by Agrobacterium tumefaciens-mediated transformation.Highest expression of cryV gene,determined by mRNA levels and insect mortality,was obtained using the CaMV 35S promoter without the gus gene configuration.Detached leaf and tuber bioassays showed a mortality rate of up to 83% and 100%,respectively,for potato tuber moth(Phthorimaea operculella Zeller) in the transgenic lines.Our results demonstrated that the presence of the gus gene negatively affects the expression level of the cryV gene.Bt expression was also facilitated by using the(ocs)3 mas super promoter,whereas the Bt expression regulated by the patatin promoter(tuber-specific) was too low to have any effect upon the mortality of potato tuber moth.These results represent significant improvement in the level of host plant resistance for the control of potato tuber moth via Bt transgenes. | Guan Hongju Li Wenbin Douches David Lu Cuihua | 2007 | 中国马铃薯2007，21，2： | 0 |
| 12 | A BBS4 mutation causes autosomal dominant polycystic liver disease显示文摘Autosomal dominant polycystic liver disease(ADPLD)refers to a condition characterized by the presence of numerous cholangiocytes-lined and fluid-filled cysts in the liver and the absence of polycystic kidney disease.1 Although patients with ADPLD may be asymptomatic,some patients suffer from abdominal pain,gastroesophageal reflux,and nausea,because of hepatomegaly. | Yalu Cui Wenping Xu Jinpei Liu Shuqing Liu Wei Huang Yihai Shi Xin Zhang Cuihua Lu Weifen Xie | 2024 | Genes & Diseases2024，11，1： | 0 |
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