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| 1 | H pylori (CagA) and Epstein-Barr virus infection in gastric carcinomas:Correlation with p53 mutation and c-Myc,Bcl-2 and Bax expression显示文摘AIM: To investigate the interrelationship between H pylori and Epstein-Barr virus (EBV) infection in the gastric carcinogenesis having in focus the p53 mutation and the c-Myc, Bcl-2 and Bax expression. METHODS: seventy-one gastric carcinoma tissues were assessed by polymerase chain reaction (PCR) for H pylori and in situ hybridization for EBV. c-Myc, Bcl-2 and Bax expression were detected by immunohistochemistry and single-stranded conformational polymorphism (SSCP) for p53 mutation. RESULTS: The positivity rates for H pylori and EBV were 94.4% and 8.45%, respectively. The majority of the cases displayed only the H pylori presence. All EBV positive cases were also H pylori positive. None infectious agent was observed in 5.55% of the cases. The intestinal type tumor was more frequent in the co-infected and non-infected groups. The female predominated in the non-infected group showing statistical significance (70.4% vs 29.6%, P=0.039). The Bcl-2 was only detected in the group exclusively infected by H pylori. However, c-Myc and Bax were detected in the three groups but with a low frequency in the co-infected group. Mutation of p53 was present in all groups, with the highest frequencies in the H pylori positive groups. CONCLUSION: The frequency of H pylori infection in gastric carcinomas was high. The presented data indicated that gastric carcinogenesis has different pathways depending of the presence of the two investigated infectious agents, suggesting a possible involvement of H pylori with apoptotic process. The low expression of c-Myc and Bax in the EBV-positive groups suggests that EBV may inhibit the expression of these proteins. Nevertheless, p53 mutation shows to be a relevant alteration, independent of both infectious agents. | Valeska Portela Lima Marcos Antonio Pereira de Lima Angela Rosa André Márcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Sílvia Helena Barem Rabenhorst | 2008 | World Journal of Gastroenterology2008，14，6： | 17 |
| 2 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 3 | Embolization of splenorenal shunt associated to portal vein thrombosis and hepatic encephalopathy显示文摘Hepatic encephalopathy(HE)is a cognitive disturbance characterized by neuropsychiatric alterations.It occurs in acute and chronic hepatic disease and also in patients with portosystemic shunts.The presence of these portosystemic shunts allows the passage of nitrogenous substances from the intestines through systemic veins without liver depuration.Therefore,the embolization of these shunts has been performed tocontrol HE manifestations,but the presence of portal vein thrombosis is considered a contraindication.In this presentation we show a cirrhotic patient with severe HE and portal vein thrombosis who was submitted to embolization of a large portosystemic shunt.Case report:a 57 years-old cirrhotic patient who had been hospitalized many times for persistent HE and hepatic coma,even without precipitant factors.She had a wide portosystemic shunt and also portal vein thrombosis.The abdominal angiography confirmed the splenorenal shunt and showed other shunts.The larger shunt was embolized through placement of microcoils,and the patient had no recurrence of overt HE.There was a little increase of esophageal and gastric varices,but no endoscopic treatment was needed.Since portosystemic shunts are frequent causes of recurrent HE in cirrhotic patients,portal vein thrombosis should be considered a relative contraindication to perform a shunt embolization.However,in particular cases with many shunts and severe HE,we found that one of these shunts can be safely embolized and this procedure can be sufficient to obtain a good HE recovery.In conclusion,we reported a case of persistent HE due to a wide portosystemic shunt associated with portal vein thrombosis.As the patient had other shunts,she was successfully treated by embolization of the larger shunt. | Letícia de Campos Franzoni Fábio Cardoso de Carvalho Rafael Gomes de Almeida Garzon Fábio da Silva Yamashiro Laís Augusti Lívia Alves Amaral Santos Mariana de Souza Dorna Júlio Pinheiro Baima Talles Bazeia Lima Carlos Antonio Caramori Giovanni Faria Silva Fernando Gomes Romeiro | 2014 | World Journal of Gastroenterology2014，20，42： | 6 |
| 4 | Biological princi- ples for future internet architecture design显示文摘 | S Balasubramaniam K Leibnitz P Lio | 2011 | IEEE Conmau- nications Magazine2011，49，7： | 1 |
| 5 | Natriuretic peptides as predictors of non-sudden and sudden cardiac death after acute myo- cardial infarction in the beta-blocking era显示文摘 | TAPANAINEN J M LINDGREN K S MAKIKAL- LIO T H | 2004 | J Am Coll Cardiol2004，43，： | 1 |
| 6 | Steam reforming of methanol over a CuO/ZnO/Al 2 O 3 catalyst part II: A carbon membrane reactor显示文摘 | Sandra Sá José M. Sousa Adélio Mendes | 2011 | Chemical Engineering Science2011，，22： | 1 |
| 7 | Selection of regulatory T cells in the thymus 显示文摘 | HSIEH C S LEE H M LIO C W | 2012 | Nat Rev Immunol2012，12，3： | 1 |
| 8 | Hydrogen production by methanol steam reforming in a membrane reactor: Palladium vs carbon molecular sieve membranes显示文摘 | Sandra Sá Hugo Silva José M. Sousa Adélio Mendes | 2009 | Journal of Membrane Science2009，，1： | 1 |
| 9 | Feasibility of robotic pancreati- coduodenectomy显示文摘 | Boggi U Signori S de Lio N | 2013 | Br J Surg2013，100，7： | 1 |
| 10 | A two-step process for thymic regulatory T cell development显示文摘 | Lio C W Hsieh C S | 2008 | Immunity2008，28，： | 1 |
| 11 | Reinforced Concrete Jacketing-Interface Influence on Cyclic Loading Response显示文摘 | Júlio Eduardo N B S Branco Fernando A B | 2008 | ACI Structural Journal2008，，4： | 1 |
| 12 | Inflammation, ageing and cancer 显示文摘 | Vasto S Carruba G Lio D Colonna-Romano G Di-Bona D Candore G | 2009 | Mech Ageing Dev2009，130，12： | 1 |
| 13 | Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method显示文摘 | Tiago M. Martins Olívia C. Pedro Rubina A. Caldeira Virgílio E. do Rosário Luís Neves Ana Domingos | 2008 | Veterinary Parasitology2008，，3： | 1 |
| 14 | A Robuat and Fast Bacteria Counting Method using CdSe/ZnS core/shell Quantum Dots as Labels显示文摘 | FU X HOANG K LIO S | 2009 | J Microbiol Methods2009，79，3： | 1 |
| 15 | Chemical Composition, Leishmanicidal and Cytotoxic Activities of the Essential Oils from Mangifera indica L. var. Rosa and Espada显示文摘 | Eduardo H. S. Ramos Marcílio M. Moraes Laís L. de A. Nerys Silene C. Nascimento Gardênia C. G. Milit?o Regina C. B. Q. de Figueiredo Cláudio A. G. da Camara Teresinha Gon?alves Silva Silvia R. B. Uliana | 2014 | BioMed Research International2014，，： | 1 |
| 16 | Production of fructooligosaccharides in high yields using a mixed enzyme system of ( - fructofuranosidase and glucose oxidase 显示文摘 | Sheu D C Lio P J Chen S T | 2001 | Biotechnology letters2001，23，18： | 1 |
| 17 | A novel granular sludge sequencing, batch reactor for removal of organic and nitrogen flora wastewater 显示文摘 | Yang S F Tay J H Lio Y | 2003 | Journal of Biotechnology2003，106，1： | 1 |
| 18 | The chemo- kine fractalkine inhibits Fas-mediated cell death of brain micro- glia 显示文摘 | Boehme S A Lio F M Maciejewski-Lenoir D | 2000 | J Immunol2000，165，1： | 1 |
| 19 | Nebulized 0.5, 2.5 and 5 ml l -epinephrine for post-extubation stridor in children: a prospective, randomized, double-blind clinical trial显示文摘 | Paulo Sérgio Lucas Silva Marcelo Cunio Machado Fonseca Simone Brasil Oliveira Iglesias Emílio Lopes Junior Vania Euzébio Aguiar Werther Brunow Carvalho | 2012 | Intensive Care Medicine2012，，2： | 1 |
| 20 | 查看详情显示文摘 | Ildo L S H&lio Tatizawa Francisco A M | | 0，，： | 1 |
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