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| 1 | Causes and consequences of microsatellite instability in gastric carcinogenesis显示文摘Loss of DNA mismatch repair(MMR) function, due to somatic or germline epi/genetic alterations of MMR genes leads to the accumulation of numerous mutations across the genome, creating a molecular phenotype known as microsatellite instability(MSI). In gastric cancer(g C), MSI occurs in about 15% to 30% of the cases. This review summarizes the current knowledge on the molecular mechanisms underlying the acquisition of MSI in g C as well as on the clinic, pathologic and molecular consequences of the MSI phenotype. Additionally, current therapeutic strategies for g C and their applicability in the MSI subset are also discussed. | Sérgia Velho Maria Sofia Fernandes Marina Leite Ceu Figueiredo Raquel Seruca | 2014 | World Journal of Gastroenterology2014，20，44： | 14 |
| 2 | Titanium dioxide induced inflammation in the small intestine显示文摘AIM:To investigate the effects of titanium dioxide (TiO2) nanoparticles (NPTiO 2 ) and microparticles (MPTiO 2 ) on the inflammatory response in the small intestine of mice. METHODS: Bl 57/6 male mice received distilled water suspensions containing TiO 2 (100 mg/kg body weight) as NPTiO 2 (66 nm), or MPTiO 2 (260 nm) by gavage for 10 d, once a day; the control group received only distilled water. At the end of the treatment the duodenum, jejunum and ileum were extracted for assessment of cytokines, inflammatory cells and titanium content. The cytokines interleukin (IL)-1b, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, IL-17, IL-23, tumor necrosis factor-α (TNF-α), intracellular interferon-γ (IFN-γ) and transforming growth factor-β (TGF-β) were evaluated by enzyme-linked immunosorbent assay in segments of jejunum and ileum (mucosa and underlying muscular tissue). CD4 + and CD8 + T cells, natural killer cells, and dendritic cells were evaluated in duodenum, jejunum and ileum samples fixed in 10% formalin by immuno-histochemistry. The titanium content was determined by inductively coupled plasma atomic emission spectrometry. RESULTS: We found increased levels of T CD4 + cells (cells/mm 2 ) in duodenum:NP 1240 ± 139.4, MP 1070 ± 154.7 vs 458 ± 50.39 (P < 0.01); jejunum:NP 908.4 ± 130.3, MP 813.8 ± 103.8 vs 526.6 ± 61.43 (P < 0.05); and ileum:NP 818.60 ± 123.0, MP 640.1 ± 32.75 vs 466.9 ± 22.4 (P < 0.05). In comparison to the control group, the groups receiving TiO 2 showed a statistically significant increase in the levels of the inflammatory cytokines IL-12, IL-4, IL-23, TNF-α, IFN-γ and TGF-β. The cytokine production was more pronounced in the ileum (mean ± SE):IL-12: NP 33.98 ± 11.76, MP 74.11 ± 25.65 vs 19.06 ± 3.92 (P < 0.05); IL-4: NP 17.36 ± 9.96, MP 22.94 ± 7.47 vs 2.19 ± 0.65 (P < 0.05); IL-23: NP 157.20 ± 75.80, MP 134.50 ± 38.31 vs 22.34 ± 5.81 (P < 0.05); TNFα: NP 3.71 ± 1.33, MP 5.44 ± 1.67 vs 0.99± 019 (P < 0.05); IFNγ: NP 15.85 ± 9.99, MP 34.08 ± 11.44 vs 2.81 ± 0.69 (P < 0.05); and TGF-β: NP 780.70 ± 318.50, MP 1409.00 ± 502.20 vs 205.50 ± 63.93 (P < 0.05). CONCLUSION:Our findings indicate that TiO2 particles induce a Th1-mediated inflammatory response in the small bowel in mice. | Carolina Maciel Nogueira Walter Mendes de Azevedo Maria Lucia Zaidan Dagli Sérgio Hiroshi Toma AndréZonetti de Arruda Leite Maria Laura Lordello Iêda Nishitokukado Carmen Lúcia Ortiz-Agostinho Maria IrmaSeixas Duarte Marcelo Alves Ferreira Aytan Miranda Sipahi | 2012 | World Journal of Gastroenterology2012，18，34： | 8 |
| 3 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 4 | Phytoplankton functional groups as indicators of human impacts along the River Loire (France)显示文摘 | András Abonyi Maria Leit?o Anne Marie Lan?on Judit Padisák | 2012 | Hydrobiologia2012，，1： | 2 |
| 5 | Development of hepatorenal syndrome in bile duct ligated rats显示文摘AIM: To evaluate in bile duct ligated rats whether there were progressive alterations of renal function without changes in histopathology. METHODS: Male Wistar rats were submitted to sham-surgery or bile duct ligation (BDL) and divided according to the post-procedure time (2, 4 and 6-wk). To determine renal function parameters, rats wereplaced in metabolic cages and, at the end of the experiment, blood and urine samples were obtained. Histology and hydroxyproline content were analyzed in liver and renal tissue. RESULTS: Rats with 2 wk of BDL increased free water clearance (P = 0.02), reduced urinary osmolality (P = 0.03) and serum creatinine (P = 0.01) in comparison to the sham group. In contrast, rats at 6 wk of BDL showed features of HRS, including signif icant increase in serum creatinine and reductions in creatinine clearance, water excretion and urinary sodium concentration. Rats with 4 wk of BDL exhibited an intermediate stage of renal dysfunction. Progressive hepatic f ibrosis according to post-procedure time was confirmed by histology. The increased levels of liver hydroxyproline contrasted with the absence of structural changes in the kidney, as assessed by histology and unchanged hydroxyproline content in renal tissue. CONCLUSION: Our data show that BDL produced progressive renal dysfunction without structural changes in the kidney, characterizing HRS. The present model will be useful to understand the pathophysiology of HRS. | Regina M Pereira Robson AS dos Santos Eduardo A Oliveira Virgínia HR Leite Filipi LC Dias Alysson S Rezende Lincoln P Costa Lucíola S Barcelos Mauro M Teixeira Ana Cristina Sim■es e Silva | 2008 | World Journal of Gastroenterology2008，14，28： | 2 |
| 6 | Helicobacter pylori Induces Increased Expression of Toll‐Like Receptors and Decreased Toll‐Interacting Protein in Gastric Mucosa that Persists Throughout Gastric Carcinogenesis显示文摘 | Pedro Pimentel‐Nunes Nádia Gon?alves Inês Boal‐Carvalho Luís Afonso Paula Lopes Roberto Roncon‐Albuquerque Rui Henrique Luís Moreira‐Dias Adelino F. Leite‐Moreira Mário Dinis‐Ribeiro | 2012 | Helicobacter2012，，1： | 2 |
| 7 | Transmission capacity: availability, maximum, transfer and reliability显示文摘 | Leite da S A M de Carvalho C J G da Fonseca M L A | 2002 | IEEE Transactions on Power Systems2002，17，3： | 1 |
| 8 | Laminated three-dimensional biodegradable foams for use in tissue engineering显示文摘 | Mikos A G Sarakinos G Leite S M | 1993 | Biomaterials1993，14，： | 1 |
| 9 | Laboratory evaluation of recycled construction and demolition waste for pavements显示文摘 | Leite F D Motta R S Kamilla L V | 2011 | Construction and Building Materials2011，25，6： | 1 |
| 10 | Performance of anaerobic reactors in the biostabilization of organic solid wastes显示文摘 | Lopes W S Leite V D Prasad S | 2003 | Journal of Solid Waste Technology and Management2003，29，2： | 1 |
| 11 | Brown pigment of the nanopowder spinel ferrite prepared by combustion reaction显示文摘 | COSTA A C F M LEITE A M D FERREIRA H S | | 0，，： | 1 |
| 12 | External validation of a classification for methylene blue magnification chromoendoscopy in premalignant gastric lesions显示文摘 | Miguel Areia Pedro Amaro Mário Dinis-Ribeiro Maria Augusta Cipriano Carol Marinho Altamiro Costa-Pereira Carlos Lopes Luís Moreira-Dias José Manuel Rom?ozinho Hermano Gouveia Diniz Freitas Maximino Correia Leit?o | 2008 | Gastrointestinal Endoscopy2008，，7： | 1 |
| 13 | Probabilistic load flow considering dependence between input nodal powers显示文摘 | Leite D S Arienti V L Allan RN | 1984 | IEEE Transactions on Power Apparatus and Systems1984，103，6： | 1 |
| 14 | Oral clefts,consanguinity,parental tobacco and alcohol use:a case-control study in Rio de Janeiro,Brazil显示文摘 | Leite IC Koifman S | 2009 | Braz Oral Res2009，23，1： | 1 |
| 15 | Influence of inoculum on performance of anaerobic reactors for treating municipal solid waste显示文摘 | Lopes W S Leite V D Prasad S | 2004 | Bioresource Technology2004，94，： | 1 |
| 16 | Sensitivity-enhanced reflection Z-scan by oblique incidence of a polarized beam显示文摘 | Martinelli M Bian S Leite J R | | 0，，： | 1 |
| 17 | An improved LMI condition for robust D-stability of uncertain polytopic systems 显示文摘 | LEITE V J S PERES P L D | 2003 | IEEE Trans Automat Control2003，48，3： | 1 |
| 18 | Production and characteristics comparison of crude β-glucosidases produced by microorganisms Thermoascus aurantiacus e Aureobasidium pullulans in agricultural wastes显示文摘 | LEITE R S R ALVES-PRADO H F CABRAL H | | 0，，06： | 1 |
| 19 | Tracking of clinical and environmental Vibrio cholerae O1 strains by combined analysis of the presence of toxin cassette, plasmid content and ERIC PCR 显示文摘 | Mastrandrea S Leite F | 1997 | FEMS Immunol Med Microbiol1997，19，1： | 1 |
| 20 | Identifying a risk profile for thyroid cancer 显示文摘 | Ward L S Morari E C Leite J L | 2007 | Arq Bras Endocrinol Metabol2007，51，5： | 1 |
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