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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors显示文摘 | Jing Liao Zhao Wu Ying Wang Lu Cheng Chun Cui Yuan Gao Taotao Chen Lingjun Rao Siye Chen Nannan Jia Huiming Dai Shunmei Xin Jiuhong Kang Gang Pei Lei Xiao | 2008 | Cell Research2008，18，5： | 61 |
| 3 | Cold-chain food contamination as the possible origin of COVID-19 resurgence in Beijing显示文摘COVID-19,caused by SARS-CoV-2[1,2],has been contained in China through stringent non-pharmaceutical interventions.Border control and quarantine have effectively prevented the virus from being spread by infected travellers,but the risk of resurgence caused by other routes of introduction and transmission remains unclear,and current strategies to prevent resurgence could be flawed.Since July,SARS-CoV-2 RNA contaminations in frozen food imported from countries with ongoing epidemics have been reported in nine provinces in China[3,4].However,there is no robust evidence of COVID-19 outbreaks initiated by environmentto-human transmission.Here we add to evidence of such transmission by investigating the recent COVID-19 resurgence in Beijing. | Xinghuo Pang Lili Ren Shuangsheng Wu Wentai Ma Jian Yang Lin Di Jie Li Yan Xiao Lu Kang Shichang Du Jing Du Jing Wang Gang Li Shuguang Zhai Lijuan Chen Wenxiong Zhou Shengjie Lai Lei Gao Yang Pan Quanyi Wang Mingkun Li Jianbin Wang Yanyi Huang Jianwei Wang COVID-19 Field Response Group COVID-19 Laboratory Testing Group | 2020 | National Science Review2020，7，12： | 51 |
| 4 | Preventive effect of ulinastatin and gabexate mesylate on post-endoscopic retrograde cholangiopancreatography pancreatitis显示文摘背景 endoscopic 以后后退 cholangiopancreatography 胰腺炎(锐气) 被认为是之一使人烦恼的复杂并发症内视镜后退 cholangiopancreatography (ERCP ) 。在锐气上评估 ulinastatin 和 gabexate mesylate (美国通用汽车公司) 的预防效果的使随机化的控制试用的结果是矛盾的。现在的学习被设计与使随机化的控制试用(RCT ) 的元分析在锐气上评估 ulinastatin 和美国通用汽车公司的预防效果 .Methods 五个电子数据库被在找在锐气上评估 ulinastatin 和美国通用汽车公司的预防效果的 RCT。概括效果被估计,方法由 Cochrane Collaboration.Results 推荐了包含 5105 个参加者的 12 研究在我们的元分析被包括。ulinastatin 的管理减少了仅仅在足够的剂量的锐气的发生(或, 0.39;95% CI, 0.19 ~ 0.81;P=0.01 ) 。需要到对待(NNT ) 的数字是 6。并且 ulinastatin 的管理也减少了 post-ERCP hyperamylasemia (PEHA ) 的发生(或, 0.40;95% CI, 0.28 ~ 0.58;P < 0.000 01 ) 。高剂量的美国通用汽车公司的慢注入为锐气预防是有效的(或, 0.44; 95% CI, 0.25 ~ 0.79;P=0.006 ) ,并且低剂量的美国通用汽车公司的快速的注入也为锐气预防显示出功效(或, 0.37; 95% CI, 0.20 ~ 0.69;P=0.002 ) 。NNT 分别地是 7 和 6。然而,在低剂量并且由慢注入的美国通用汽车公司的管理是无效的(或, 0.99;95% CI, 0.64 ~ 1.55;P=0.98 ) 。美国通用汽车公司的管理有趋势减少 PEHA 率,然而并非到统计意义(或, 0.86;95% CI, 0.73 ~ 1.01;P=0.06 ) 。当低质量的研究被排除时,有二高质量的研究的元分析显示 ulinastatin 没减少锐气的率(或, 0.63;95% CI, 0.32 ~ 1.26;P=0.19 ) 并且 PEHA 发生(或, 0.80;95% CI, 0.31 ~ 2.07;P=0.64 ) 。有六高质量的研究的元分析证明美国通用汽车公司管理减少了锐气发生(或, 0.52;95% CI, 0.29 ~ 0.91; P=0.02 ) 不为 PEHA 预防是有效的(或, 0.88;95% CI, 0.74 ~ 1.04;P=0.12 ).Conclusions Ulinastatin 和美国通用汽车公司可能具有为锐气的预防的价值。美国通用汽车公司应该在高剂量并且由快速的注入被管理。并且 ulinastatin 的剂量应该是足够的。然而,结论不是 overwhelming.More 大样品的尺寸和高质量的 RCT 仍然被需要阐明二药的政府是否确实穿上预防效果锐气。 | ZHANG Zhi-feng YANG Ning ZHAO Gang ZHU Lei ZHU Ying WANG Li-xia | 2010 | Chinese Medical Journal2010，，18： | 38 |
| 5 | Trustworthiness of Internet-based software显示文摘Recent years see an increasing concern over the trustworthiness of Inter- net-based software. By analyzing the trustworthiness of Internet-based software and the nature of the Internet applications, we point out that, on the one hand, due to the open- ness and dynamic nature of the Internet, the identity trustworthiness and the capability trustworthiness of the software are facing serious challenges; on the other hand, in order to ensure the trustworthiness of the whole system, emerging computing paradigms based on the collaboration of autonomous software need some impacts on the behavior of the software. Here we put forward a conceptual model for the trustworthiness of Inter- net-based software, and propose a trustworthy assurance framework for Internet-based virtual computing environment (iVCE). This framework deals with the trustworthy proper- ties of software on identity, capability and behavior in a combinated way. The authorization management in inter-domain computing environment, assurance on high availability of service and incentive mechanism for autonomic collaboration are taken as three core mechanisms of iVCE trustworthy assurance. | WANG Huaimin TANG Yangbin YIN Gang LI Lei | 2006 | Science in China(Series F)2006，49，6： | 28 |
| 6 | Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture显示文摘To investigate the biological character of human adipose-derived adult stem cells (hADAS cells) when cultured in vitro and the relationship between hADAS cell’s replication activity and the donor’s age factor, and to assess the stem cells as a new source for tissue engineering. hADAS cells are isolated from human adipose tissue of different age groups (from adolescents to olds: <20 years old, 21―40 years old, 41―60 years old and >61 years old groups). The protein markers (CD29, CD34, CD44, CD45, CD49d, HLA-DR, CD106) of hADAS cells were detected by flow cytometry (FCM) to identify the stem cell, and the cell cycle was examined for P20 hADAS cells to evaluate the safety of the subculture in vitro. The generative activity of hADAS cells in different age groups was also examined by MTT method. The formula “ log2T D = t logN t ? logN 0” was used to get the time doubling (TD) of the cells. The results showed that the cells kept heredity stabilization by chromosome analysis for at least 20 passages. The TD of these cells increased progressively by ageing, and the TD of the <20 years old group was lower than that of the >61 years old group (statistical analysis of variance (ANOVA), P=0.002, P<0.05). These find- ings suggested that a higher level of hADAS cells replication activity was found in the younger dona- tors, and they represent novel and valuable seed cells for studies of tissue engineering. | LEI Lei, LIAO WeiMing, SHENG PuYi, FU Ming, HE AiShan & HUANG Gang Department of Orthopedics, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China | 2007 | Science China(Life Sciences)2007，50，3： | 25 |
| 7 | Latest Progress of the Chinese Meteorological Satellite Program and Core Data Processing Technologies显示文摘In this paper,the latest progress,major achievements and future plans of Chinese meteorological satellites and the core data processing techniques are discussed.First,the latest three FengYun(FY)meteorological satellites(FY-2H,FY-3D,and FY-4A)and their primary objectives are introduced Second,the core image navigation techniques and accuracies of the FY meteorological satellites are elaborated,including the latest geostationary(FY-2/4)and polar-orbit(FY-3)satellites.Third,the radiometric calibration techniques and accuracies of reflective solar bands,thermal infrared bands,and passive microwave bands for FY meteorological satellites are discussed.It also illustrates the latest progress of real-time calibration with the onboard calibration system and validation with different methods,including the vicarious China radiance calibration site calibration,pseudo invariant calibration site calibration,deep convective clouds calibration,and lunar calibration.Fourth,recent progress of meteorological satellite data assimilation applications and quantitative science produce are summarized at length.The main progress is in meteorological satellite data assimilation by using microwave and hyper-spectral infrared sensors in global and regional numerical weather prediction models.Lastly,the latest progress in radiative transfer,absorption and scattering calculations for satellite remote sensing is summarized,and some important research using a new radiative transfer model are illustrated. | Peng ZHANG Qifeng LU Xiuqing HU Songyan GU Lei YANG Min MIN Lin CHEN Na XU Ling Sun Wenguang BAI Gang MA Di XIAN | 2019 | Advances in Atmospheric Sciences2019，36，9： | 26 |
| 8 | Effects of molasses on the fermentation characteristics of mixed silage prepared with rice straw, local vegetable by-products and alfalfa in Southeast China显示文摘This experiment was conducted to study the effect of molasses on the fermentation characteristics of mixed silage ensiled rice straw and vegetable by-products with alfalfa.Mixture(202 g kg^(–1) dry matter(DM))consisting of rice straw,broccoli residue and alfalfa at the ratio of 5:4:1 was ensiled with three experimental treatments:(1)no additives(control);(2)molasses at 2.5%(M1);(3)molasses at 5%(M2)on a fresh matter basis of mixture,respectively.All treatments were packed into laboratory-scale silos,and three silos per treatment were sampled on days 1,3,5,14 and 30.The result showed that the p H value of all mixed silages decreased gradually with the time of ensiling except for the control silage,in which a significant increase(P<0.05)on day 30 occurred.The lactic acid content increased gradually with the time of ensiling and reached the highest value on day 14,and a marked decrease(P<0.05)was found in the control silage on day 30.The changes of acetic acid content showed similar pattern with lactic acid content.A trace amount of propionic and butyric acid contents were found in the three mixed silages during the fermentation period.Comparing to the control,M1 and M2 treatments improved the fermentation quality of mixed silages as indicated by higher(P<0.05)lactic acid contents and lower(P<0.05)p H and ammonia-N contents.The Flieg points also showed that M1 and M2 silages were well preserved,whereas the control silage had a bad quality.Overall,the findings of this study suggested that adding molasses could improve fermentation quality of mixed silage,and M1 was more suitable for practical application. | WANG Jian CHEN Lei YUAN Xian-jun GUO Gang LI Jun-feng BAI Yun-feng SHAO Tao | 2017 | Journal of Integrative Agriculture2017，16，3： | 24 |
| 9 | Coastal erosion in China under the condition of global climate change and measures for its prevention显示文摘The general characteristics of coastal erosion in China are described in terms of the regional geography, the form of erosion, the causes of erosion, and the challenges we are facing. The paper highlights the relationship between coastal erosion and sea level rises, storm waves and tides, and the influence of global climate changes on coastal erosion along the coastal zone of China. The response of the risk of coastal erosion in China to climate changes has obvious regional diversity. Research into and the forecasting of the effects of climate changes on coastal erosion are systemic work involving the natural environment, social economy, and alongshore engineering projects in the global system. Facing global warming and continual enhancement of coastal erosion, suggestions for basic theoretical study, prevention technology, management system assurance, and strengthening the legal system are presented here. | Feng Cai Xianze Su Jianhui Liu Bing Li Gang Lei | 2009 | Progress in Natural Science:Materials International2009，19，4： | 23 |
| 10 | Exosomes containing miR-21 transfer the characteristic of cisplatin resistance by targeting PTEN and PDCD4 in oral squamous cell carcinoma显示文摘 | Tao Liu Gang Chen Dawei Sun Minghui Lei Yongqiang Li Changming Zhou Xiaodong Li Wei Xue Hong Wang Chunjun Liu Jiang Xu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，9： | 22 |
| 11 | Pathways of China's PM;air quality 2015–2060 in the context of carbon neutrality显示文摘Clean air policies in China have substantially reduced particulate matter(PM;) air pollution in recent years,primarily by curbing end-of-pipe emissions.However,reaching the level of the World Health Organization(WHO) guidelines may instead depend upon the air quality co-benefits of ambitious climate action.Here,we assess pathways of Chinese PM;air quality from 2015 to 2060 under a combination of scenarios that link global and Chinese climate mitigation pathways(i.e.global 2℃-and 1.5℃-pathways,National Determined Contributions(NDC) pledges and carbon neutrality goals) to local clean air policies.We find that China can achieve both its near-term climate goals(peak emissions) and PM;air quality annual standard(35 μg/m;) by 2030 by fulfilling its NDC pledges and continuing air pollution control policies.However,the benefits of end-of-pipe control reductions are mostly exhausted by 2030,and reducing PM;exposure of the majority of the Chinese population to below 10 μg/m;by 2060 will likely require more ambitious climate mitigation efforts such as China’ s carbon neutrality goals and global1.5℃-pathways.Our results thus highlight that China’s carbon neutrality goals will play a critical role in reducing air pollution exposure to the level of the WHO guidelines and protecting public health. | Jing Cheng Dan Tong Qiang Zhang Yang Liu Yu Lei Gang Yan Liu Yan Sha Yu Ryna Yiyun Cui Leon Clarke Guannan Geng Bo Zheng Xiaoye Zhang Steven JDavis Kebin He | 2021 | National Science Review2021，8，12： | 23 |
| 12 | Nature:骨髓微环境发生突变可导致造血干细胞异常显示文摘白血病骨髓微环境中支持血液发育的某些DNA突变可以驱动周围造血干细胞形成白血病,来自艾默里大学温希普癌症研究所和亚特兰大儿童健康中心的研究人员在国际学术期刊Nature上公布了他们的最新研究进展。许多致癌突变都作用于细胞自身。让细胞自身生长更快。相比之下,努南综合征小鼠模型上可以观察到间接的邻近细胞效应,努南综合征是一种能够增加白血病患病风险的遗传紊乱。 | Lei Dong, Wen-Mei Yu, Hong Zheng, Melinda Pauly, Muxiang Zhou Cheng-Kui Qu Mignon L. Loh Silvia T. Bunting Gang Huang Hal E. Broxmeyer David T. Scadden | 2016 | 现代生物医学进展2016，16，35： | 18 |
| 13 | Recent advances in gel polymer electrolyte for high-performance lithium batteries显示文摘Lithium batteries (LBs) have become increasingly important energy storage systems in our daily life. However, their practical applications are still severely plagued by the safety issues from liquid electrolyte, especially when the batteries are exposed to mechanical, thermal, or electrical abuse conditions. Gel polymer electrolytes (GPEs) are being considered as an effective solution to replace currently available organic liquid electrolyte for building safer LBs. This review provides recent advancements in GPEs applied for high-performance LBs. On the one hand, from the environmental and economic point of view, the skeletons of GPEs changed from traditional polymer to renewable and degradable polymer. On the other hand, in addition to being as a component with good electrochemical and physical characterizations, the GPEs also need to provide some functions for addressing the concerns of lithium (Li) dendrites, unstable cathode electrolyte interface, dissolution and migration of transition metal ions,'shuttle effect' of polysulfides, and so on. Finally, to synchronously meet the challenges from the advanced cathode and Li metal anode, the bio-based GPEs with multi-functionality are proposed to develop high-energy/powerdensity batteries in the future. | Ming Zhu Jiaxin Wu Yue Wang Mingming Song Lei Long Sajid Hussain Siyal Xiaoping Yang Gang Sui | 2019 | Journal of Energy Chemistry2019，28，10： | 17 |
| 14 | Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells and Schwann cells显示文摘背景最重要的目的在受伤针的绳索移植研究是为 axonal 生长提供有利环境。而且,新接枝的持续发现正在提供新潜在地有趣移植候选人。我们的目的是观察神经干细胞(NSC ) 的合作移植的词法、功能的修理效果, Schwann 装壁板圩(SC ) 并且 poly rats.Methods 的针的绳索损害上的 lactide-co-glycolide 酸(PLGA ) PLGA 的脚手架被制作。NSC 和 SC 是有教养的,与用 5-bromodeoxyuridine 标记的 NSC,并且 NSC/PLGA 或 NSC+SCs/PLGA 的建筑群被构造。36 只 Wistar 老鼠随机被划分成三个组:组织 A (PLGA 的移植) ,组织 B (NSC/PLGA 的移植) 并且组织 C (NSC+SCs/PLGA 的移植) 。正确 hemicord 的 3 公里长度在所有老鼠在显微镜下面被移开。PLGA 或 PLGA-celIs 的建筑群被植入进损害地点。Basso-Beattie-Bresnahan (BBB ) 运动分数,马达和更低的手足潜在的唤起的 somatosensory 被检验听说康复感觉并且在在损害以后的 4 个星期, 8 个星期, 12 个星期和 24 个星期的马达功能。所有恢复针的绳索损害( SCI )纸巾被观察与他染色, immunohistochemistry ,和 transelectronmicroscopy 到在 4 个星期识别移植房间和神经纤维的新生的幸存,迁居和区别, 8 个星期,在到在损害以后的 24 个星期的从 4 个星期的 injury.Results ( 1 )以后的 12 个星期和 24 个星期,移植房间的组的 BBB 运动分数比 non-cell-transplanted 组的那些好,特别组 C ( P 0.05 )。somatosensory 的振幅唤起了潜力(9 月) ,唤起马达的潜力(MEP ) 在组 B 和 C 在损害以后被改进,但是在 4 个星期的 9 月和 MEP 的振幅在损害以后在 12 个星期和 24 个星期是比那低的。与组 B 相比,在组 C 的 9 月和 MEP 的振幅被改进。9 月和 MEP 的振幅没在组 A 在损害以后被改进。(2 ) 他染色表明支架的体积减少了,在支架的房间的数字增加了。成长得最新的 capillaries 能也被看见。染色的 Immunohistochemistry 证明移植 NSC 能幸存并且移居直到 24 个星期和他们能区分进神经原和 oligodendrocytes。改革轴突与 transelectronmicroscopy 在支架房间建筑群被观察。上述表明在在损害,和他们能区分进各种各样的神经房间以后,移植 NSC 能在老鼠的针的绳索熬过并且移居直到 24 个星期的组 C.Conclusions 是更广泛的。cells/PLGA 的合作移植能支持受伤针的绳索的功能的恢复。共同移植的效果 NSC+SCs/PLGA 比独自移植 NSC/PLGA 好。 | CHEN Gang HU Yan-rong WAN Hong XIA Lei LI Jun-hua YANG Fei QU Xue WANG Shen-guo WANG Zhong-cheng | 2010 | Chinese Medical Journal2010，，17： | 16 |
| 15 | An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis显示文摘MYB 蛋白质在真核细胞的有机体起重要作用。在植物, R1R2R3 类型 MYB 蛋白质在房间周期控制工作。然而, R2R3 类型 MYB 蛋白质是否也涉及房间部门过程,仍然保持未知。这里,我们报导那 R2R3 类型抄写因素基因, AtMYB59,涉及房间周期前进和根生长的规定。AtMYB59 蛋白质在洋葱的原子核是局部性的表皮的房间并且 transactivation 活动。在酵母房间的 AtMYB59 的表示压制房间增长,和 transformants 与更长的房间有更多的原子核和更高的 aneuploid DNA 内容。在 AtMYB59 的保存领域的变化在酵母细胞生长上废除它的效果。在同步 Arabidopsis 房间暂停, AtMYB59 基因明确地在房间周期前进期间在 S 阶段被表示。表示和 promoter-GUS 分析表明 AtMYB59 基因富有地在根被表示。转基因的植物 overexpressing AtMYB59 更短的根与野类型的植物(Arabidopsis 就职 Col-0 ) 相比,并且在在根尖端的有丝分裂的房间的一半附近在中期。相反地,空变异的 myb59-1 比关口在中期让更长的根和更少有丝分裂的房间,建议那 AtMYB59 可以由扩大有丝分裂的房间的中期禁止根生长。AtMYB59 调整许多下游的基因,包括 CYCB1; 1 基因,可能通过到 MYB 应答的元素的绑定。这些结果在细胞周期规定和植物根生长为 AtMYB59 支持一个角色。 | Rui-Ling Mu Yang-Rong Cao Yun-Feng Liu Gang Lei Hong-Feng Zou Yong Liao Hui-Wen Wang Wan-Ke Zhang Biao Ma Ji-Zhou Du Ming Yuan Jin-Song Zhang Shou-Yi Chen | 2009 | Cell Research2009，19，11： | 16 |
| 16 | The global significance of biodiversity science in China: an overview显示文摘Biodiversity science in China has seen rapid growth over recent decades,ranging from baseline biodiversity studies to understanding the processes behind evolution across dynamic regions such as the Qinghai-Tibetan Plateau.We review research,including species catalogues;biodiversity monitoring;the origins,distributions,maintenance and threats to biodiversity;biodiversity-related ecosystem function and services;and species and ecosystems'responses to global change.Next,we identify priority topics and offer suggestions and priorities for future biodiversity research in China.These priorities include(i)the ecology and biogeography of the Qinghai-Tibetan Plateau and surrounding mountains,and that of subtropical and tropical forests across China;(ii)marine and inland aquatic biodiversity;and(iii)effective conservation and management to identify and maintain synergies between biodiversity and socio-economic development to fulfil China's vision for becoming an ecological civilization.In addition,we propose three future strategies:(i)translate advanced biodiversity science into practice for biodiversity conservation;(ii)strengthen capacity building and application of advanced technologies,including high-throughput sequencing,genomics and remote sensing;and(iii)strengthen and expand international collaborations.Based on the recent rapid progress of biodiversity research,China is well positioned to become a global leader in biodiversity research in the near future. | Xiangcheng Mi Gang Feng Yibo Hu Jian Zhang Lei Chen Richard T.Corlett Alice C.Hughes Stuart Pimm Bernhard Schmid Suhua Shi Jens-Christian Svenning Keping Ma | 2021 | National Science Review2021，8，7： | 16 |
| 17 | Co-transplantation of neural stem cells and Schwann cells within poly (L-lactic-co-glycolic acid) scaffolds facilitates axonal regeneration in hemisected rat spinal cord显示文摘 | XIA Lei WAN Hong HAO Shu-yu LI De-zhi CHEN Gang GAO Chuan-chuan LI Jun-hua YANG Fei WANG Shen-guo LIU Song | 2013 | Chinese Medical Journal2013，，5： | 15 |
| 18 | Differential stem cell aging kinetics in Hutchinson-Gilford progeria syndrome and Werner syndrome显示文摘Hutchinson-Gilford 早衰症候群(HGPS ) 和沃纳症候群(WS ) 是二最好描绘的人的 progeroid 症候群。HGPS 被一个点变化在 lamin A (LMNA ) 基因引起,导致截断的蛋白质 productprogerin 的生产。WS 被变化在 WRN 基因引起,编码 loss-of-function RecQ DNA helicase。这里,由基因编辑,我们创造了 isogenic 人的胚胎的干细胞(转换字符) 与异质接合(G608G/+) 或同型结合(G608G/G608G ) LMNA 变化和 biallelic WRN 大美人为为 HGPS 和 WS 致病建模,分别地。当转换字符和 endothelial 房间(EC ) 没介绍早熟的老朽的任何特征时, HGPS 间充质、 WS 间充质的干细胞(MSC ) 与不同动力学显示出联系老化的显型。当 HGPS-MSCs 展出了迟了发作的尖锐早衰 characterisitcs 时, WS-MSCs 有早发作的温和早衰显型。一起拿,我们的学习比较并且形成对照不同病理 underpinning 二早衰混乱,并且提供可靠干细胞为病理学、生理的老化识别新治疗学的策略的基于的模型。 | Zeming Wu Weiqi Zhang Moshi Song Wei Wang Gang Wei Wei Li Jinghui Lei Yu Huang Yanmei Sang Piu Chan Chang Chen Jing Qu Keiichiro Suzuki Juan Carlos Izpisua Belmonte Guang-Hui Liu | 2018 | Protein & Cell2018，9，4： | 15 |
| 19 | Self-microemulsifying drug delivery system for improving the bioavailability of huperzine A by lymphatic uptake显示文摘Huperzine A(Hup-A) is a poorly water-soluble drug with low oral bioavailability. A selfmicroemulsifying drug delivery system(SMEDDS) was used to enhance the oral bioavailability and lymphatic uptake and transport of Hup-A. A single-pass intestinal perfusion(SPIP) technique and a chylomicron flow-blocking approach were used to study its intestinal absorption, mesenteric lymph node distribution and intestinal lymphatic uptake. The value of the area under the plasma concentration–time curve(AUC) of Hup-A SMEDDS was significantly higher than that of a Hup-A suspension(P <0.01).The absorption rate constant(K_a) and the apparent permeability coefficient(P_(app)) for Hup-A in different parts of the intestine suggested a passive transport mechanism, and the values of K_a and P_(app) of Hup-A SMEDDS in the ileum were much higher than those in other intestinal segments. The determination of Hup-A concentration in mesenteric lymph nodes can be used to explain the intestinal lymphatic absorption of Hup-A SMEDDS. For Hup-A SMEDDS, the values of AUC and maximum plasma concentration(C_(max)) of the blocking model were significantly lower than those of the control model(P<0.05). The proportion of lymphatic transport of Hup-A SMEDDS and Hup-A suspension were about 40% and 5%,respectively, suggesting that SMEDDS can significantly improve the intestinal lymphatic uptake and transport of Hup-A. | Fang Li Rongfeng Hu Bin Wang Yun Gui Gang Cheng Song Gao Lei Ye Jihui Tang | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 15 |
| 20 | bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis显示文摘Iron(Fe)deficiency is prevalent in plants grown in neutral or alkaline soil.Plants have evolved sophisticated mechanisms that regulate Fe homeostasis,ensuring survival.In Arabidopsis,FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(FIT)is a crucial regulator of Fe-deficiency response.FIT is activated indirectly by basic helix-loop-helix(bHLH)IVc transcription factors(TFs)under Fed eficiency;how ever,it remains unclear which protein(s)act as the linker to mediate the activation of FIT by bHLH IVc TFs.In this study,we characterize the functions of bHLH121 and demonstrate that it directly associates with the FIT promoter.We found that loss-of-function mutations of bHLH121 cause severe Fedeficiency symptoms,reduced Feaccum ulation,and disrupted expression of genes associated with Fehomeostasis.Genetic analysis showed that FIT is epistatic to bHLH121 and FIT overexpression partially rescues the bhlh121 mutant.Further investigations revealed that bHLH IVc TFs interact with and promote nuclear accumulation of bHLH121.We demonstrated that bHLH121 has DNA-binding activity and can bind the prom oters of the FIT and bHLHlb genes,but we did not find that it has either direct transcriptional activation or repression activity tow ard these genes.Meanw hile,we found that bHLH121 functions downstream of and is a direct target of bHLH IVc TFs,and its expression is induced by Fe deficiency in a bHLH IV c-dependent manner.Taken together,these results establish that bHLH121 functions together with bHLH IVc TFs to positively regulate the expression of FIT and thus plays a pivotal role in maintaining Fe homeostasis in Arabidopsis. | Rihua Lei Yang Li Yuerong Cai Chenyang Li Mengna Pu Chengkai Lu Yujie Yang Gang Liang | 2020 | Molecular Plant2020，13，4： | 14 |
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