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| 1 | Precise base editing of non-allelic acetolactate synthase genes confers sulfonylurea herbicide resistance in maize显示文摘Single-nucleotide polymorphisms contribute to phenotypic diversity in maize. Creation and functional annotation of point mutations has been limited by the low efficiency of conventional methods based on random mutation. An efficient tool for generating targeted single-base mutations is desirable for both functional genomics and precise genetic improvement. The objective of this study was to test the efficiency of targeted C-to-T base editing of two non-allelic acetolactate synthase(ALS) in generating sulfonylurea herbicide-resistant mutants. A CRISPR/Cas9 nickase-cytidine deaminase fused with uracil DNA glycosylase inhibitor(UGI) was employed to achieve targeted conversion of cytosine to thymine in ZmALS1 and ZmALS2. Both protoplasts and recovered mutant plants showed the activity of the cytosine base editor, with an in vivo efficiency of up to 13.8%. Transgene-free edited plants harboring a homozygous ZmALS1 mutation or a ZmALS1 and ZmALS2 double mutation were tested for their resistance at a dose of up to 15-fold the recommended limit of chlorsulfuron, a sulfonylurea herbicide widely used in agriculture. Targeted base editing of C-to-T per se and a phenotype verified in the generated mutants demonstrates the power of base editing in precise maize breeding. | Yanmin Li Jinjie Zhu Hao Wu Changlin Liu Changling Huang Jinhao Lan Yanming Zhao Chuanxiao Xie | 2020 | The Crop Journal2020，8，3： | 9 |
| 2 | Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain显示文摘Peripheral nerve injury could lead to chronic neuropathic pain.Understanding transcriptional changes induced by nerve injury could provide fundamental insights into the complex pathogenesis of neuropathic pain.Gene expression profiles of dorsal root ganglia(DRG)in neuropathic pain condition have been studied.However,little is known about transcriptomic changes in individual DRG neurons after peripheral nerve injury. | Kaikai Wang Sashuang Wang Yan Chen Dan Wu Xinyu Hu Yingjin Lu Liping Wang Lan Bao Changlin Li Xu Zhang | 2021 | Cell Research2021，31，8： | 5 |
| 3 | Publisher Correction: Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain显示文摘 | Kaikai Wang Sashuang Wang Yan Chen Dan Wu Xinyu Hu Yingjin Lu Liping Wang Lan Bao Changlin Li Xu Zhang | 2021 | Cell Research2021，31，8： | 1 |
| 4 | Measurement of cross sections for (n,2n),(n,p) and (n,a) reactionson germanium isotopes induced by 14 MeV neutrons显示文摘 | Lan Changlin Xu Xiaosan Fang Kaihong | 2008 | Annals of Nuclear Energy2008，35，： | 1 |
| 5 | Particle identification using CsI(Tl) crystal with three different methods显示文摘Three pulse-shape-discrimination (PSD) methods are applied to study the particle identification (PID) by using CsI(Tl) crystal, especially for identifying light charged particles. The zero-cross time method, fast and total component method and signal rise time method are used. The experiment, data analysis and results are compared. Good PID for p, α and γ can be achieved with a CsI(Tl)-photomultiplier assembly. | LAN Changlin RUAN Xichao LIU Gang KONG Xiangzhong ZHOU Zuying | 2008 | Nuclear Science and Techniques2008，19，6： | 0 |
| 6 | Determination of the half-life of^(91)Sr in uranium sample bombarded by 14.8 MeV neutron显示文摘Purpose Due to the few reports on the^(91)Sr half-life previously,the accuracy and uncertainty of the results are difficult to meet the requirements such as calculating the cross section and thefission yields of 235U,238U,and 239Pu.To solve the discrepancy of^(91)Sr half-life in existing experimental data,the half-life of^(91)Sr was measured in this work,and the uncertainty was discussed in detail.Method The measurements were performed by the activation method implemented for the uranium sample using the K-400 neutron generator at the Chinese Academy of Engineering Physics(CAEP).The half-life of^(91)Sr was measured byγ-ray spectrometry using the high-resolution High Purity Germanium(HPGe)Detector.Results Through exponential functionfitting and a detailed discussion of the uncertainty evaluation,the half-life of^(91)Sr in the present work was determined to be 9.65±0.30 h,which was consistent with the values reported previously and the uncertainty was reduced greatly,however,the uncertainty assessment of the latter was barely documented.Conclusions In this work,a more accurate measurement of the^(91)Sr half-life is provided,as well as the uncertainty is discussed in detail.This result will provide essential information for applications using an activation method,such as the calculation offission yields of 235U,238U,and 239Pu. | Yuting Wei Changlin Lan Li Feng Chao Xu Xianlin Yang Xiaojun Li Jiahao Wang Yujie Ge Benchao Lou Li Jiang | 2022 | Radiation Detection Technology and Methods2022，6，3： | 0 |
| 7 | Chemical Synthesis of Structurally Defined Phosphorylated Ubiquitins Suggests Impaired Parkin Activation by Phosphorylated Ubiquitins with a Non-Phosphorylated Distal Unit显示文摘Mutations in genes encoding PINK1(PTEN-induced kinase 1)and Parkin(E3 ubiquitin ligase)are identified in familial Parkinson’s disease.However,it remains unclear whether the phosphorylated Ub chains activate wild-type Parkin(w-Parkin)or phosphorylated Parkin(p-Parkin),with the consequent expulsion of the damaged mitochondria. | Man Pan Qingyun Zheng Shuai Gao Qian Qu Yuanyuan Yu Ming Wu Huan Lan Yulei Li Sanling Liu Jiabin Li Demeng Sun Lining Lu Tian Wang Wenhao Zhang Jiawei Wang Yiming Li Hong-Gang Hu Changlin Tian Lei Liu | 2019 | CCS Chemistry2019，1，5： | 0 |
| 8 | A direct spino-cortical circuit bypassing the thalamus modulates nociception显示文摘Nociceptive signals are usually transmitted to layer 4 neurons in somatosensory cortex via the spinothalamic-thalamocortical pathway.The layer 5 corticospinal neurons in sensorimotor cortex are reported to receive the output of neurons in superficial layers;and their descending axons innervate the spinal cord to regulate basic sensorimotor functions.Here,we show that a subset of layer 5 neurons receives spinal inputs through a direct spino-cortical circuit bypassing the thalamus,and thus define these neurons as spino-cortical recipient neurons(SCRNs).Morphological studies revealed that the branches from spinal ascending axons formed a kind of disciform structure with the descending axons from SCRNs in the basilar pontine nucleus(BPN).Electron microscopy and calcium imaging further confirmed that the axon terminals from spinal ascending neurons and SCRNs made functional synaptic contacts in the BPN,linking the ascending sensory pathway to the descending motor control pathway.Furthermore,behavioral tests indicated that the spino-cortical connection in the BPN was involved in nociceptive responses.In vivo calcium imaging showed that SCRNs responded to peripheral noxious stimuli faster than neighboring layer 4 cortical neurons in awake mice.Manipulating activities of SCRNs could modulate nociceptive behaviors.Therefore,this direct spino-cortical circuit represents a noncanonical pathway,allowing a fast sensory-motor transition of the brain in response to noxious stimuli. | Bing Cai Dan Wu Hong Xie Yan Chen Huadong Wang Sen Jin Yuran Song Anan Li Shiqi Huang Sashuang Wang Yingjin Lu Lan Bao Fuqiang Xu Hui Gong Changlin Li Xu Zhang | 2023 | Cell Research2023，33，10： | 0 |
| 9 | IL-24/MDA-7 Suppressed the Transplantation Tumor in BALB/c Mice显示文摘It is well-documented that interleukin-24 (IL-24) can induce apoptosis in a large spectrum of human cancer derived cell lines, but the effect of MDA-7/IL-24 gene transfer on mouse melanoma cells remains unknown. The eukaryotic expressing plasmid of IL-24 (pEGFP-IL-24) was constructed by DNA recombination technique. The recombination plasmid and empty vector were transfected into B16F0 cells and the expressions of IL-24 were determined by LSM, the proliferation of B16F0 cells was measured by MTT assay, and apoptosis rate and cell-cycle distribution of B16F0 cells were measured by FCM. The inhibitory effect of IL-24 gene transfection in mouse solid tumor was observed and measured. Compared with the control, the proliferation of B16F0 cells was inhibited by transfection with pEGFP-IL-24 and the G2/M phase of the transfected cells was also increased. Moreover, the percentage of mice with detectable tumor was decreased after inoculated with B16F0 cells transfected with pEGFP-IL-24. Growth rate of tumor in mouse model was significantly inhibited in IL-24 gene therapy group compared with the control. Proliferation of B16F0 cells was inhibited by pEGFP-IL-24 transfection. The intratumor injection of pEGFP-IL-24 could inhibit the growth of solid tumor in mice remarkably. | Jiancheng Xue Changlin Wu Yi Zhu Lan Li Xintang Dang Shaoguang Qu Fang Liu | 2006 | Cellular & Molecular Immunology2006，3，5： | 0 |
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