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| 1 | MicroRNAs in the prognosis and therapy of colorectal cancer: From bench to bedside显示文摘MicroRNAs(miRNAs) are small, single-stranded, noncoding RNAs that can post-transcriptionally regulate the expression of various oncogenes and tumor suppressor genes. Dysregulated expression of many miRNAs have been shown to mediate the signaling pathways critical in the multistep carcinogenesis of colorectal cancer(CRC). MiR NAs are stable and protected from RNase-mediated degradation, thereby enabling its detection in biological fluids and archival tissues for biomarker studies. This review focuses on the role and application of miRNAs in the prognosis and therapy of CRC. While stage Ⅱ CRC is potentially curable by surgical resection, a significant percentage of stage Ⅱ CRC patients do develop recurrence. MiRNA biomarkers may be used to stratify such high-risk population for adjuvant chemotherapy to provide better prognoses. Growing evidence also suggests that miRNAs are involved in the metastatic process of CRC. Certain of these miRNAs may thus be used as prognostic biomarkers to identify patients more likely to have micro-metastasis, who could be monitored more closely after surgery and/or given more aggressive adjuvant chemotherapy. Intrinsic and acquired resistance to chemotherapy severely hinders successful chemotherapy in CRC treatment. Predictive miRNA biomarkers for response to chemotherapy may identify patients who will benefit the most from a particular regimen and also spare the patients from unnecessary side effects. Selection of patients to receive the new targeted therapy is becoming possible with the use of predictive miRNA biomarkers. Lastly, forced expression of tumor suppressor miRNA or silencing of oncogenic miRNA in tumors by gene therapy can also be adopted to treat CRC alone or in combination with other chemotherapeutic drugs. | Kenneth KW To Christy WS Tong Mingxia Wu William CS Cho | 2018 | World Journal of Gastroenterology2018，24，27： | 29 |
| 2 | 室内氡与肺癌关系研究显示文摘目的 直接调查居室水平氡浓度对居民肺癌危险度的影响 ,并与由矿工受照结果推导的结论相比较。方法 在甘肃省平凉、庆阳两地区进行病例 对照研究。该地区居民稳定而室内氡水平较高。肺癌病例必须是在 1994年 1月至 1998年 4月期间诊断 ,年龄在 30~ 75岁之间 ,居住在平凉和庆阳两地区的居民。根据人口调查资料随机选取对照 ,对照要在年龄、性别、地区方面与病例相匹配。在过去 5~ 30年期间 ,凡住过两年以上的房间都布放了氡探测器。氡测量涵盖了 77%的照射周期。结果 病例居室的平均氡浓度为 2 30 4Bq m3 (n =76 8) ,对照为 2 2 2 2Bq m3 (n =16 5 9)。居民肺癌危险度随室内氡水平的增高有所上升 (P <0 0 0 1)。根据线性模型 ,对全部研究个体而言 ,在 10 0Bq m3 情况下 ,附加比值比 (EOR)为 0 19(95 %CI:0 0 5 ,0 47)。对测量涵盖了 10 0 %照射周期的个体 ,为 0 31(95 %CI:0 10 ,0 81)。在对照射的不确定度进行调整后 ,估算值可增加70 %。结论 研究结果证明室内水平氡照射会增加肺癌危险度 。 | 王作元 Lubin JH 王陇德 张守志 Boice JD Jr 崔宏星 张淑蓉 Conrath SC 夏英 尚兵 Brenner A 雷苏文 Metayer KC 曹吉生 Chen KW 雷淑杰 Kleinerman RA | 2002 | 中华放射医学与防护杂志2002，22，3： | 28 |
| 3 | 迷迭香提取物的抗乳腺癌活性研究显示文摘目的 : 研究天然香料迷迭香提取物 ( rosemary extract)及其主要抗氧化组分的抗乳腺癌活性。方法 : 采用常规的 MTT法 ,考察迷迭香提取物及其主要抗氧化组分鼠尾草酸( carnosic acid) ,鼠尾草酚 ( carnosol) ,rosmanol和迷迭香酸 ( rosmarinic acid)等对扩散型大鼠乳腺癌细胞 F3II和人乳腺癌细胞 MCF- 7增殖的抑制活性。结果 : ( 1 )鼠尾草酚和 rosmanol对乳腺癌细胞增殖抑制较强 ,鼠尾草酸和迷迭香提取物对乳腺癌细胞增殖抑制较弱 ,而迷迭香酸在该实验条件下基本上对增殖无抑制活性。 ( 2 )鼠尾草酚是一个选择性很高的乳腺癌细胞增殖抑制剂 ,其对乳腺癌细胞呈现较强的抑制活性 ,而对正常乳腺细胞增殖则基本上无抑制活性或抑制活性很低。结论 : 迷迭香主要抗氧化组份鼠尾草酚和 rosmanol对乳腺癌细胞增殖呈现较强的抑制活性 ,其中鼠尾草酚的抑制活性具有良好的选择性 ,是一个极有希望的乳腺癌增殖抑制剂 。 | 曹树稳 余燕影 温辉梁 Singletary KW | 2001 | 营养学报2001，23，3： | 23 |
| 4 | Insulin glargine versus NPH insulin therapy in Asian Type 2 diabetes patients显示文摘 | Pan, CY Sinnassamy, P Chung, KD Kim, KW | 2007 | 中国生物学文摘2007，21，11： | 17 |
| 5 | Proteomic analysis of roots growth and metabolic changes under phosphorus deficit in maize (Zea mays L.) plants显示文摘 | Li, KP Xu, CZ Zhang, KW Yang, AF Zhang, JR | 2007 | 中国生物学文摘2007，21，11： | 16 |
| 6 | The performance characteristics of prostate-specific antigen and prostate-specific antigen density in Chinese men显示文摘我们在中国人调查了前列腺特定的抗原(PSA ) 和 PSA 密度(PSAD ) 的表演特征。经历了 transrectal 的所有中国人到 2013 的从 2000 年的指导超声的前列腺活体检视(TRUS-PB ) 被包括。为 PSA 和 PSAD 操作特征(巨鸟) 曲线的接收装置被分析。敏感,特性,积极预兆的价值(PPV ) 和在不同截止层次的否定预兆的价值(NPV ) 是计算的。2606 个中国人的一个总数被包括。为 ROC,在曲线下面的区域为 PSA 是 0.770 (P <0.001 ) 并且 0.823 为 PSAD (P <0.001 ) 。4.5 ng ml −1 的 PSA 94.4% 的有的敏感, 14.1% 的特性, 29.5% 的 PPV,和 86.9% 的 NPV;0.12 ng ml −1 cc −1 的 PSAD 有 94.5% 的敏感, 26.6% 的特性, 32.8% 的 PPV,和 92.7% 的 NPV。在逻辑回归分析的 multivariate 上,在 4.5 ng ml −1 截止的 PSA (或 1.61, 95% CI 1.05-2.45, P = 0.029 ) 并且在 0.12 ng ml −1 cc 截止的 PSAD −1( 或 6.22, 95% CI 4.20-9.22, P <0.001 ) 是为 TRUS-PB 上的前列腺癌症察觉的重要预言者。在结论,在在中国人的不同截止层次的 PSA 和 PSAD 的表演与在白种人的那些很不同。4.5 ng ml −1 的 PSA 和 0.12 ng ml −1 cc −1 的 PSAD 在 95% 敏感附近有并且是在中国人的前列腺癌症察觉的重要预言者。 | Jeremv YC Teoh Steffi KK Yuen James HL Tsu Charles KW Wong Brian SH Ho Ada TL Ng Wai-Kit Ma Kwan-Lun Ho Ming-Kwong Yiu | 2017 | Asian Journal of Andrology2017，19，1： | 13 |
| 7 | Bis(7)-tacrine attenuates beta amyloid-induced neuronal apoptosis by regulating L-type calcium channels显示文摘 | Fu H Li W Lao Y Luo J Lee NT Kan KK Tsang HW Tsim KW Pang Y Li Z Chang DC Li M Han Y | 2006 | 中国生物学文摘2006，20，10： | 10 |
| 8 | 自体腘绳肌肌腱与新鲜冷冻同种异体胫前肌肌腱移植对前交叉韧带重建效果的影响显示文摘随着内固定装置和更加精确的隧道技术的发展,自体腘绳肌肌腱和同种异体胫前肌肌腱移植重建前交叉韧带(anterior cruciate ligament,ACL)开始普遍应用于关节外科。尽管自体组织移植具有很高的成功率,但一些外科医师还是倾向于采用同种异体移植物,以避免供区并发症;但同种异体移植物的有效性目前仍存在争议。本研究旨在通过前瞻性多中心随机对照实验研究,比较自体腘绳肌肌腱与新鲜冷冻同种异体胫前肌肌腱移植重建ACL在临床疗效和膝关节稳定性方面的差异。 | Lawhorn KW Howell SM Traina SM 沈洪园 黄华扬 | 2012 | 中国骨科临床与基础研究杂志2012，4，5： | 9 |
| 9 | AKT and ERK1/2 signaling in intrahepatic cholangiocarcinoma显示文摘Intrahepatic cholangiocarcinomas (ICC) are neoplasms that originate from cholangiocytes and can occur at any level of the biliary tree. Surgical resection is the current therapy of choice for this highly aggressive cancer. However, the 5-year survival still is poor, with high recurrence rates. Due to the intrahepatic growth a signifi cant proportion of patients present with advanced disease and are not candidates for curative surgery or transplantation. The existing palliative options are of limited benefit and there is a great necessity for novel therapeutic options. In this article we review the role of the phosphoinositide 3-kinase(PI3K)/ AKT and extracellular regulated kinase (ERK) signaling pathways in ICC and present new data on the prognostic value of these protein kinases. Finally, we discuss future upcoming therapeutic options based on targeting these signaling pathways. | KJ Schmitz H Lang J Wohlschlaeger GC Sotiropoulos H Reis KW Schmid HA Baba | 2007 | World Journal of Gastroenterology2007，13，48： | 8 |
| 10 | Screening for gastric cancer in Asia: current evidence and practice显示文摘 | Wai K Leung Ming-shiang Wu Yasuo Kakugawa Jae J Kim Khay-guan Yeoh Khean Lee Goh Kai-chun Wu Deng-chyang Wu Jose Sollano Udom Kachintorn Takuji Gotoda Jaw-town Lin Wei-cheng You Enders KW Ng Joseph JY Sung | 2008 | Lancet Oncology2008，，3： | 7 |
| 11 | 评定染色物匀染性的客观方法显示文摘一、导论染色不均匀在染整生产中一般是不能接受的,除非是石磨、酸洗或颜料染色的纺织品,因为它是依照流行趋势来寻求效果的。评定均匀发性(或不均匀性)的传统方法是通过目测或与类似的例如对同一批织物的样品比较,测定颜色的差异。这些方法多少带有主观性。我们试图应用分光光度技术,确定一个相对不均匀指数(RUI),以此来评定染色样品的不均匀程度。这一指数使我们有可能定量地表示均匀与不均匀度。二、相对不均匀指数(RUI) | CL Chong SQ Liang KW Yeung 洪瑞熔 | 1994 | 丝绸技术1994，2，2： | 4 |
| 12 | 经直肠超声引导下穿刺活检诊断前列腺癌与直肠指检和前列腺特异性抗原水平的关系:在中国人群中的情况如何?显示文摘我们分析了经直肠超声引导下穿刺活检后前列腺癌的检出率与直肠指检和前列腺特异性抗原水平的关系以及中国人群检出前列腺癌的危险因素。我们从数据库中检索了自2000年到2013年所有首次接受经直肠超声引导下前列腺穿刺活检的中国男性的数据。结合直肠指检的发现以及前列腺特异性抗原水平(〈 4, 4-10, 10.1-20,20.1-50 , 〉 50ng/ml)分析了前列腺癌的检出率。使用多因素Logistic回归研究了前列腺癌检出的潜在危险因素。共有2606中国男性人群纳入该研究。在直肠指检正常的患者中,不同前列腺特异性抗原水平(〈 4, 4-10,10.1-20, 20.1-50, 〉 50 ng/ml)患者前列腺癌的检出率分别为8.6%,13.4%,21.8%,41.7%和85.2%。在直肠指检异常的患者中,不同前列腺特异性抗原水平(〈 4, 4-10, 10.1-20, 20.1-50, 〉 50 ng/ml)患者前列腺癌的检出率分别为12.4%, 30.2%, 52.7%, 80.6%和96.4%。多因素Logistic回归分析提示:老龄、较小的前列腺体积、更多针数的穿刺活检、直肠指检异常发现、较高的PSA水平都与增大的前列腺癌检出风险相关(P 〈0.001)。与西方人群相比,中国男性人群的前列腺癌检出率较低。考虑到不同的危险因素,可以采用个体化的方式来决定是否采取经直肠超声引导下穿刺活检。 | Jeremy YC Teoh Steffi KK Yuen James HL Tsu Charles KW Wong Brian SH Ho Ada TL Ng Wai-Kit Ma Kwan-Lun Ho Ming-Kwong Yiu | 2015 | Asian Journal of Andrology2015，17，5： | 4 |
| 13 | Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells显示文摘AIM: To development of an improved p38 MAPK inhibitor-based serum-free medium for embryoid body cardiomyocyte differentiation of human pluripotent stem cells. METHODS: Human embryonic stem cells (hESC) differentiated to cardiomyocytes (CM) using a p38 MAPK inhibitor (SB203580) based serum-free medium (SB media). Nutrient supplements known to increase cell viability were added to SB medium. The ability of these supplements to improve cardiomyogenesis was evaluated by measurements of cell viability, total cell count, and the expression of cardiac markers via flow cytometry. An improved medium containing Soy hydrolysate (HySoy) and bovine serum albumin (BSA) (SupSB media) was developed and tested on 2 additional cell lines (H1 and Siu-hiPSC). Characterization of the cardiomyocytes was done by immunohistochemistry, electrophysiology and quantitative real-time reverse transcriptionpolymerase chain reaction. RESULTS: hESC cell line, HES-3, differentiating in SB medium for 16 d resulted in a cardiomyocyte yield of 0.07 ± 0.03 CM/hESC. A new medium (SupSB media) was developed with the addition of HySoy and BSA to SB medium. This medium resulted in 2.6 fold increase in cardiomyocyte yield (0.21 ± 0.08 CM/hESC). The robustness of SupSB medium was further demonstrated using two additional pluripotent cell lines (H1, hESC and Siu1, hiPSC), showing a 15 and 9 fold increase in cardiomyocyte yield respectively. The age (passage number) of the pluripotent cells did not affect the cardiomyocyte yields. Embryoid body (EB) cardiomyocytes formed in SupSB medium expressed canonical cardiac markers (sarcomeric α-actinin, myosin heavy chain and troponin-T) and demonstrated all three major phenotypes: nodal-, atrial- and ventricular-like. Electrophysiological characteristics (maximum diastolic potentials and action potential durations) of cardiomyocytes derived from SB and SupSB media were similar. CONCLUSION: The nutrient supplementation (HySoy and BSA) leads to increase in cell viability, cell yield and cardiac marker expression during cardiomyocyte differentiation, translating to an overall increase in cardiomyocyte yield. | Sherwin Ting Marti Lecina Yau-Chi Chan Hung Fat Tse Shaul Reuveny Steve KW Oh | 2013 | World Journal of Stem Cells2013，5，3： | 4 |
| 14 | Screening for gastric cancer in Asia: current evidence and practice显示文摘 | Wai K Leung Ming-shiang Wu Yasuo Kakugawa Jae J Kim Khay-guan Yeoh Khean Lee Goh Kai-chun Wu Deng-chyang Wu Jose Sollano Udom Kachintorn Takuji Gotoda Jaw-town Lin Wei-cheng You Enders KW Ng Joseph JY Sung | 2008 | Lancet Oncology2008，，3： | 3 |
| 15 | 甲状腺术中喉返神经监测技术的标准化显示文摘目的在甲状腺手术中缺少术中神经监测(intra operative neuromonitoring,IONM)的标准化操作可导致结果变异性强,这些结果可产生错误信息并增加喉返神经损伤的危险性。因此有必要进行IONM操作的标准化。方法本研究共招募了289例进行过甲状腺切除术的患者(435根神经有危险),均由一位外科医师实施手术。每例患者均由同一位麻醉师使用EMG气管导管进行插管。每例患者均进行标准化IONM操作。该操作包括术前和术后对声带运动进行录像监测、保证电极在正确位置、喉返神经剥离前后刺激迷走神经并记录EMG信号,并摄像记录暴露的喉返神经。结果5例患者出现IONM波形异常,是由于电极错位所致,这一问题被立刻监测到。监测到1例患者在手术较早阶段出现非喉返神经损伤。甲状腺剥离时18例患者的神经失去了EMG信号,使用我们的标准化IONM操作后神经损伤的原因得以清楚阐明。结论标准化IONM操作不仅在消除错误的IONM结果方面有用且有帮助,而且有助于阐明喉返神经损伤的机制。在确定外科手术的缺陷并提高外科手术技巧后,本研究显著降低了神经麻痹的发生率。 | Chiang FY Lee KW | 2011 | 中国医学文摘(耳鼻咽喉科学)2011，26，1： | 3 |
| 16 | Evaluation of the string test for the detection of Helicobacter pylori显示文摘AIM: Helicobacter pylori can be diagnosed by invasive ornon-invasive tests but to obtain bacteria for culture andantibiotic susceptibility testing, an upper GI endoscopy isoften required. The string test may be a minimally-invasivealternative method of obtaining H. pylori samples. This studyevaluates the sensitivity and specificity of the string test inthe diagnosis of H. pylori in comparison with endoscopicmeans of diagnosis.METHODS: This was a prospective open comparative studyof patients with dyspepsia with endoscopy-based tests asgold standard (defined as a positive CLO test and antralhistology). Fasting patients swallowed the encapsulated-string(Entero-test Hp), which was withdrawn after 1 hour. Thegastric juice from the string was plated onto H. pylori-selectivemedia for culture. Helicobacter pylori was identified by typicalcolony morphology, gram stain and biochemical test results.RESULTS: Thirty dyspeptic patients were recruited of whom21 (70 %) were positive for H. pylori according to the goldstandard. The sensitivity, specificity, positive predictive value,and negative predictive value for the string test were 38 %,100 %, 100 % and 41% respectively, and for endoscopicbiopsies 81%, 100 %, 100 %, 69 % respectively (P=0.004).Logistic regression showed that only abundant growthdensity from endoscopic biopsy cultures to be a predictor ofa positive string test (P=0.018).CONCLUSION: The string test is an alternative method toendoscopy in obtaining H. pylori but has a low sensitivitycompared to endoscopic biopsies. | Rupert WL Leong Ching C Lee Thomas KW Ling WaiK Leung Joseph JY Sung | 2003 | World Journal of Gastroenterology2003，9，2： | 3 |
| 17 | Skeletal myoblast based delivery of angiogenic growth factors:a comparison between angiopoietin-1 and VEGF gene delivery for therapeutic angiogenesis in the heart显示文摘Objectives This study investigated the efficacy of human skeletal myoblasts (SkM) mediated either human vascular endothelial growth factor-165 (hVEGF165) or angiopoietin-1 (Ang-1) on vascular development and myocardial regional perfusion. Methods A porcine heart model of chronic infarction was created in 28 female swine by coronary artery ligation. The animals were randomized into: (1) group-1, DMEM injected (n=6), (2) group-2, Ad-null transduced SkM transplanted (n=6), (3) group-3, Ad-hVEGF165 transduced SkM transplanted (n=8), and (4) group-4, Ad-Ang-1 transduced SkM (n=8). Three weeks later, 5 ml DMEM containing 3×108 SkM carrying exogenous genes were intramyocardially injected into 20 sites in left ventricle in groups-2, -3 and -4. Animals in group-1 were injected 5 ml DMEM without cells. Animals were kept on 5 mg/kg cyclosporine per day for 6 weeks. Regional blood flow was measured using fluorescent microspheres. The heart was explanted at 2, 6 and 12 weeks after transplantation for histological studies. Results Histological examination showed survival of lac-z expressing myoblasts in host tissue. Capillary density based on Von Willebrand factor-VIII (vWF-VIII) at low power field (×100) was 57.13±11.85 in group-3 at 6 weeks and declined to 32.1±5.21 at 12 weeks, while it was 39.9±10.26 at 6 weeks and increased to 45.14±6.54 at 12 weeks in group-4. The mature blood vessel index was highest in group- 4 at 6 and 12 weeks after transplantation. The regional blood flow in the center and peri-infarct area was significantly increased in animals of groups-3 and -4. Conclusions SkM carrying either hVEGF165 or Ang-1 induced neovascularization with increased blood flow. Ang-1 overexpression resulted in mature and stable blood vessel formation and may be a more potent arteriogenic inducer for neovascularization. | Husnain Kh Haider In-Chin Song Peter K Law Eugene KW Sim | 2006 | Journal of Geriatric Cardiology2006，3，3： | 3 |
| 18 | Dose dependent induction of transforming growth factor beta (TGF-beta) in the lung tissue of fibrosis-prong mice after thoracic irradiation显示文摘 | Rube CE Uthe D Schmid KW | 1999 | Int J Radiol1999，21，9： | 2 |
| 19 | Rivaroxaban versus warfarin in nonvalvular atrial fibrillation显示文摘 | Patel MR Mahaffey KW Garg J | | New England Journal of Medicine0，，： | 2 |
| 20 | Lessons from hereditary colorectal cancer显示文摘 | Kinzler KW Vogelsein B | | 0，，： | 2 |
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