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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Physics of Silicene Stripes显示文摘 | A. Kara C. Léandri M. E. Dávila P. Padova B. Ealet H. Oughaddou B. Aufray G. Lay | 2009 | Journal of Superconductivity and Novel Magnetism2009，，： | 3 |
| 3 | Physics and chemistry of silicene nano-ribbons显示文摘 | G. Le Lay B. Aufray C. Léandri H. Oughaddou J.-P. Biberian P. De Padova M.E. Dávila B. Ealet A. Kara | 2009 | Applied Surface Science2009，，： | 2 |
| 4 | Hepatitis C virus' Achilles' heel - dependence on liverspecific microRNA miR-122显示文摘 | Kara L Norman Peter Sarnow | 2010 | Cell Research2010，20，3： | 2 |
| 5 | Mothers'Intentions to Breastfeed and Hospital Practices on Breastfeeding:A Longitudinal Study at 6 Months After Birth on Predictors of Breastfeeding in a Cohort of Mothers From a Large Northern New England Medical Center显示文摘 | Kara L Kaikini Kristiina Hyrkas | 2014 | Journal of Obstetric2014，43，1： | 1 |
| 6 | Sequences and factors: a guide to MHC class Ⅱ transcription显示文摘 | Glimcher L H Kara C J | 1992 | Annual Review of Immunology1992，10，： | 1 |
| 7 | Combining geometry and domain knowledge to interpret hand-drawn diagrams 显示文摘 | Gennari L Kara L B Stahovich T F | 2005 | Computers & Graphics (S0097-8493)2005，29，4： | 1 |
| 8 | Medical com plications during stroke rehabilitation显示文摘 | Yu G Wilson K | 1995 | St-roke1995，26，6： | 1 |
| 9 | US -guided place-ment of temporary internal jugular vein catheters : immediate tech- nical success and complications in normal and high - risk patients 显示文摘 | OGUZKURT L TERCAN F KARA G | 2005 | EurJ Radiol2005，55，1： | 1 |
| 10 | Does the prevalent mutation 677C → T in the methylene-tetrahydrofolate reductase gene account for hyperhomocysteinemia related to cardiovascular disease显示文摘 | Chadefaux-Vekemans B Kara Mostefa A Thuillier L | | 0，，1： | 1 |
| 11 | Changes in phytohormone status in stems and roots after decapitation of pea seedlings显示文摘 | KOTOVA L M KOTOV A A KARA A N | 2004 | Russian Journal of Plant Physiolo- gy2004，51，1： | 1 |
| 12 | Adams Effects of physical activity ,body weight and composition and muscular strength on bone density in young women显示文摘 | KARA L MADSEN WILLIAM C | 1998 | Medicine and Science in Sports and Exercise1998，3，1： | 1 |
| 13 | Granulomatous mastitis:is it an autoimmune disease? Diagnostic and therapeutic dilemmas 显示文摘 | Ozel L Unal E Kara M | 2011 | Surg Today2011，10，： | 1 |
| 14 | Human vascular cmooth muscle cells contains functional estrogen receptor显示文摘 | Karas Bruce L | 1994 | Circulation1994，89，： | 1 |
| 15 | Long-term safety and efficacy of a combination of niacin extended release and simvastatin in patients with dyslipidemia显示文摘 | Karas R H Kashyap M L Knopp R H | 2008 | Am J Cardiovasc Drugs2008，8，2： | 1 |
| 16 | US-guided placement of temporary internal jugular vein catheters:immediate technical success and complications in normal and high-risk patients显示文摘 | Oguzkurt L Tercan F Kara G | 2005 | Eur J Radiol2005，55，： | 1 |
| 17 | Choosing a scholarlyJournal during manuscript submission: the way how it rings true for physiatrists显示文摘 | Ozai Franchignoni F Kara M | 2012 | E urJ Phys Rehabil Med2012，48，4： | 1 |
| 18 | Space charge measurements on epoxy resin filled with microandnano-sized particles显示文摘 | Motyl E Moron L Karas K | 2008 | Przeglad Elektrotechniczny2008，84，10： | 1 |
| 19 | Hydrogels for the Repair of Articular Cartilage Defects 显示文摘 | Kara L Spiller Suzanne A | 2011 | Tissue Engineering2011，17，4： | 1 |
| 20 | Systemic antibiotic prophylaxis after gastro intestinal hemorrhage in cirrhotic patients with high risk of infection 显示文摘 | PAUWELS A MO STEFA KARA N DEBENES B et a l | 1996 | Hepatology1996，24，4： | 1 |
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