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| 1 | Genetic diversity of rice cultivars (Oryza sativa L.) in China and the temporal trends in recent fifty years显示文摘To understand geographical distribution of the genetic diversity of rice cultivars {Oryza sativa L.) and its trends in recent fifty years in China, 453 ’ accessions were analyzed by 36 microsatellites loci and 42 phenotypic traits. Results revealed that the genetic diversity by SSRs is highly consistent with that by phenotypic traits and the genetic diversity of indica cultivars was higher than that of japonica cultivars; the genetic diversity of cultivars declined from 1950s to 1980s and then increased greatly; among the six rice ecological zones (REZs), genetic diversity of REZTl was the highest and those of REZV and REZVT were the lowest at both DNA and phenotypic level. Jiangsu and Jiangxi provinces in the middle and lower reaches of Yangtze River and Sichuan province in southwest of China were the areas with the highest genetic diversity. Breeders in REZV which is an important japonica rice area but with very low genetic diversity should explore more gene resources to widen the genetic backgrounds of cultivars. | QI Yonawen ZHANG Dongling ZHANG Hongliang WANG Meixing SUN Junli WEI Xinghua QIU Zongen TANG Shengxiang CAO Yongsheng WANG Xiangkun LI Zichao | 2006 | Chinese Science Bulletin2006，51，6： | 27 |
| 2 | Natural Variation in CCD4 Promoter Underpins Species-Specific Evolution of Red Coloration in Citrus Peel显示文摘Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference.Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments.In this study,using an integrated genetic approach we revealed that a 5;c/s-regulatory change at CCD4b encoding CAROTENOID CLEAVAGE DIOXYGENASE 4b is a major genetic determinant of natural variation in C3 0 apocarotenoids responsible for red coloration of citrus peel.Functional analyses demonstrated that in addition the known role in synthesizing 3-citraurin,CCD4b is also responsible for the production of another important C3 0 apocarotenoid pigment,p-citraurinene.Furthermore,analyses of the CCD4b promoter and transcripts from various citrus germplasm accessions established a tight correlation between the presence of a putative 5'c/s-regulatory enhancer within an MITE transposon and the enhanced allelic expression of CCD4b in C3 0 apocarotenoid-rich red-peeled accessions.Phylogenetic analysis provided further evidence that functional diversification of CCD4b and naturally occurring variation of the CCD4b promoter resulted in the stepwise evolution of red peels in mandarins and their hybrids.Taken together,our findings provide new insights into the genetic and evolutionary basis of apocarotenoid diversity in plants,and would facilitate breeding efforts that aim to improve the nutritional and aesthetic value of citrus and perhaps other fruit crops. | Xiongjie Zheng Kaijie Zhu Quan Sun Weiyi Zhang Xia Wang Hongbo Cao Meilian Tan Zongzhou Xie Yunliu Zeng Junli Ye Lijun Chai Qiang Xu Zhiyong Pan Shunyuan Xiao Paul D.Fraser Xiuxin Deng | 2019 | Molecular Plant2019，12，9： | 18 |
| 3 | Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco显示文摘光合的公司 < 潜水艇 class= “ a-plus-plus ” > 2 固定是在地球上的器官的碳的最终的来源并且因此为庄稼生产和碳隐遁是必要的。Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco ) 催化光合的公司的第一步 < 潜水艇 class= “ a-plus-plus ” > 2 固定。然而, Rubisco 的极端低 carboxylation 效率为改进光合的效率使它成为最吸引人的目标。广泛的研究集中了于重新设计更有效的酶,而是努力被它的结构功能关系的有限理解和一个有效选择系统的缺乏向它的活动阻碍了。探讨 Rubisco 的失败的分子的工程,我们开发了在那里完全连接宿主房间的生长到 Rubisco 活动的一个 Escherichia 基于 coli 的指导活动的选择系统。Synechococcus sp。与在小子单元的 E49V 和 D82G 替换变异的 PCC7002 Rubisco 被一个轮进化从 15,000 异种的一个总数选择。这异种证明 85% 向公司在催化效率在特定的 carboxylation 活动和 45% 改进增加 < 潜水艇 class= “ a-plus-plus ” > 2 。小子单元的 E49V 变化被推测通过和大子单元的 Q225 的相互作用影响 holoenzyme 催化作用。这个相互作用从更高的植物和 Chlamydomonas reinhardtii 在各种各样的 Rubisco 之中被保存。这些的知识可能为从更高的植物设计 Rubisco 提供线索,与增加庄稼收益的潜力。 | Zhen Cai Guoxia Liu Junli Zhang Yin Li | 2014 | Protein & Cell2014，5，7： | 12 |
| 4 | Propofol differentially induces unconsciousness and respiratory depression through distinct interactions between GABAa receptor and GABAergic neuron in corresponding nuclei显示文摘Propofol is the most commonly used intravenous anesthetic worldwide.It can induce loss of consciousness prior to the occurrence of severe respiratory suppression,which is also a pharmacodynamic feature of all general anesthetics.However,the neural mechanisms underlying this natural phenomenon are controversial and highly related to patient safety.In the present study,we demonstrated that the pharmacodynamic effects of propofol(50 and 100 μM)on suppression of consciousness-related excitatory postsynaptic currents in the medial prefrontal cortex(mPFC)and centromedian nucleus of the thalamus(CMT)were lower than those in the kernel respiratory rhythmogenesis nucleus pre-Botzinger complex(PrBo).Furthermore,we unexpectedly found that the GABAa receptor β3 subunit is the key target for propofol's action and that it is mutually and exclusively expressed in GABAergic neurons.It is also more abundant in the mPFC and CMT,but mainly co-localized with GABAergic neurons in the PrBo.As a result,the differentiated expression pattern should mediate more neuron suppression through the activation of GABAergic neurons in the mPFC and CMT at low doses of propofol(50 μM).However,PrBo GABAergic neurons were only activated by propofol at a high dose(100 μM).These results highlight the detailed pharmacodynamic effects of propofol on consciousness-related and respiration-related nuclei and provide the distinct interaction mechanism between the β3 subunit and GABAergic neurons in mediating the suppression of consciousness compared to the inhibition of respiration. | Junli Jiang Yingfu Jiao Po Gao Wen Yin Wei Zhou Yunchun Zhang Yanjun Liu Daxiang Wen Yuan Wang Liang Zhou Tian Yu Weifeng Yu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，8： | 11 |
| 5 | TiO_2-loaded activated carbon fiber:Hydrothermal synthesis,adsorption properties and photo catalytic activity under visible light irradiation显示文摘TiO_2-loaded activated carbon fibers(ACF) were prepared by a hydrothermal method.The samples were characterized by scanning electron microscopy(SEM),X-ray diffraction(XRD),Fourier transform infrared(FT1R) spectrometry and UV-vis diffuse reflectance spectra(DRS).SEM images showed that the TiO_2nanoparticles were deposited on the surface of ACF,and the particle size and loading amount of TiO_2were varied by changing the initial concentration of tetrabutyl titanate(TBOT).The results of an ash experiment showed that the loading amounts of TiO_2 were 18.4%,43.3%,52.5%,75.1%,and 91.1%for initial concentrations of TBOT of 0.07,014,0.21,0.28,and 0.35 mol/L,respectively.Physical interactions played an important role in the formation of TiO_2/ACF composite fibers that absorb UV and visible light.Compared with those of ACF,improved adsorption and photocatalytic activity toward Rhodamine B(RhB)were observed for TiO_2/ACF composite fiber.The Rhodamine B could be removed efficiently by TiO_2/ACF composite fibers,and the TiO_2 loading amount had a significant effect on the photocatalytic activity of TiO_2/ACF composite fibers. | Hao Meng Wei Hou Xinxin Xu Junli Xu Xia Zhang | 2014 | Particuology2014，12，3： | 10 |
| 6 | Developing a bacteriophage cocktail for biocontrol of potato bacterial wilt显示文摘Bacterial wilt is a devastating disease of potato and can cause an 80% production loss. To control wilt using bacteriophage therapy, we isolated and characterized twelve lytic bacteriophages from different water sources in Kenya and China. Based on the lytic curves of the phages with the pathogen Ralstonia solanacearum, one optimal bacteriophage cocktail, P1, containing six phage isolations was formulated and used for studying wilt prevention and treatment efficiency in potato plants growing in pots. The preliminary tests showed that the phage cocktail was very effective in preventing potato bacterial wilt by injection of the phages into the plants or decontamination of sterilized soil spiked with R. solanacearum. Eighty percent of potato plants could be protected from the bacterial wilt(caused by R. solanacearum reference strain GIM1.74 and field isolates), and the P1 cocktail could kill 98% of live bacteria spiked in the sterilized soil at one week after spraying.However, the treatment efficiencies of P1 depended on the timing of application of the phages, the susceptibility of the plants to the bacterial wilt, as well as the virulence of the bacteria infected,suggesting that it is important to apply the phage therapy as soon as possible once there are early signs of the bacterial wilt. These results provide the basis for the development of bacteriophagebased biocontrol of potato bacterial wilt as an alternative to the use of antibiotics. | Cuihua Wei Junli Liu Alice Nyarnbura Maina Francis B Mwaura Junping Yu Chenghui Yan Ruofang Zhang Hongping Wei | 2017 | Virologica Sinica2017，32，6： | 8 |
| 7 | Geographical genetic diversity and divergence of common wild rice (O. rufipogon Griff.) in China显示文摘Using 36 SSR markers and 889 accessions of common wild rice in China, the genetic diversity and the divergence among different geographical populations are investigated. Guangdong Province has the largest number of alleles, which account for 84% of the total alleles detected in the study, followed by Guangxi Province. The Nei's gene diversity indices, from high to low, are in the sequence of Hainan, Guangdong, Guangxi, Fujian, Hunan, Jiangxi, and Yunnan provinces. Two genetic diversity centers of Chinese common wild rice are detected on the basis of geographic analysis, i.e., the region covering Boluo, Zijin, Lufeng, Haifeng, Huidong and Huiyang counties of Guangdong Province and the region covering Yongning, Longan, Laibin and Guigang counties of Guangxi Province. The common wild rice in Yunnan, Hunan, Jiangxi, and Fujian provinces are diverged into respectively independent populations with relatively large genetic distances, whereas, those in Hainan, Guangdong and Guangxi provinces have relatively low genetic divergence. Under the condition of geographic separation, natural selection is considered as one of the primary forces contributing to the divergence of common wild rice in China. | WANG MeiXing ZHANG HongLiang ZHANG DongLing PAN DaJian LI DaoYuan FAN ZhiLan QI YongWen SUN JunLi YANG QingWen LI Chen LI ZiChao | 2008 | Chinese Science Bulletin2008，53，22： | 8 |
| 8 | Distinctive effects of CD34- and CD133-specific antibody-coated stents on re-endothelialization and in-stent restenosis at the early phase of vascular injury显示文摘It is not clear what effects of CD34-and CD133-specific antibody-coated stents have on reendothelialization and in-stent restenosis(ISR)at the early phase of vascular injury.This study aims at determining the capabilities of different coatings on stents(e.g.gelatin,anti-CD133 and anti-CD34 antibodies)to promote adhesion and proliferation of endothelial progenitor cells(EPCs).The in vitro study revealed that the adhesion force enabled the EPCs coated on glass slides to withstand flow-induced shear stress,so that allowing for the growth of the cells on the slides for 48 h.The in vivo experiment using a rabbit model in which the coated stents with different substrates were implanted showed that anti-CD34 and anti-CD133 antibody-coated stents markedly reduced the intima area and restenosis than bare mental stents(BMS)and gelatin-coated stents.Compared with the anti-CD34 antibody-coated stents,the time of cells adhesion was longer and earlier present in the anti-CD133 antibody-coated stents and anti-CD133 antibody-coated stents have superiority in re-endothelialization and inhibition of ISR.In conclusion,this study demonstrated that anti-CD133 antibody as a stent coating for capturing EPCs is better than anti-CD34 antibody in promoting endothelialization and reducing ISR. | Xue Wu Tieying Yin Jie Tian Chaojun Tang Junli Huang Yinping Zhao Xiaojuan Zhang Xiaoyan Deng Yubo Fan Donghong Yu Guixue Wang | 2015 | Regenerative Biomaterials2015，2，2： | 7 |
| 9 | Mechanism of vertical crack formation in Yb_(2)SiO_(5) coatings deposited via plasma spray-physical vapor deposition显示文摘Plasma spray-physical vapor deposition(i.e.,PS-PVD)is a promising method for obtaining advanced environmental barrier coatings(EBCs).The EBCs must meet some requirements in the application,in which the thermal cycle performance affects the service lifetime.The preparation of artificial vertical cracks in Yb_(2)SiO_(5) coatings is an effective approach for meeting the requirements above because vertical cracks provide a strain tolerance.To clarify the formation mechanism of vertical cracks during the PSPVD,the effects of coating thickness and substrate temperature on the formation of vertical cracks were investigated.In addition,the interactions of spray powder and plasma flame during coating deposition were also characterized by optical spectroscopy.It is indicated that vertical cracks are formed due to a thermal expansion mismatch between Yb_(2)SiO_(5) and mullite coating,transient cooling after deposition and the nucleation of evaporated Yb_(2)SiO_(5) as well. | Xiaofeng Zhang Chao Wang Ruijun Ye Chunming Deng Xinghua Liang Ziqian Deng Shaopeng Niu Jinbing Song Guo Liu Min Liu Kesong Zhou Jian Lu Junli Feng | 2020 | Journal of Materiomics2020，6，1： | 6 |
| 10 | Assessing indica-japonica differentiation of improved rice varieties using microsatellite markers显示文摘To assess the indica-japonica differentiation of improved rice varieties, a total of 512 modern varieties including 301 indica and 211 japonica accessions were analyzed using 36 microsatellites. The Fst coefficients ranged from 0.002 to 0.730 among the loci with an average of 0.315. Significant differentiation was detected at 94.4% of the loci studied (P<0.05, pairwise Fst tests), indicating that there was a high level of indica-japonica differentiation within the improved varieties. At 18 loci, about 74%-98% of the alleles of indica and japonica accessions were distributed in two ranges of amplicon length. Linkage disequilibrium analysis showed that the distribution trends were significantly nonrandomly associated. Using the differentiation trends at the 18 loci, microsatellite index (MI) was proposed for discrimination of the two subspecies. When rice accessions with MI value greater than zero were classified as indica, and those with MI value smaller than zero were classified as japonica, about 96.1% of the accessions could be classified. This result agrees with the classification based on morphological-physiological characters, indicating that this method is feasible and effective. | Yongwen Qi Hongliang Zhang Dongling Zhang Meixing Wang Junli Sun Li Ding Fenghua Wang Zichao Li | 2009 | Journal of Genetics and Genomics2009，36，5： | 5 |
| 11 | Genetic structure and diversity of Oryza sativa L. in Guizhou,China显示文摘Preserving many kinds of rice resources and rich variations, Guizhou Province is one of the districts with the highest genetic diversity of cultivated rice (Oryza sativa L.) in China. In the current research, genetic diversity and structure of 537 accessions of cultivated rice from Guizhou were studied using 36 microsatellite markers and 39 phenotypic characters. The results showed that the model-based genetic structure was the same as genetic-distance-based one using SSRs but somewhat different from the documented classification (mainly based on phenotype) of two subspecies. The accessions being classified into indica by phenotype but japonica by genetic structure were much more than that being classified into japonica by phenotype but indica by genetic structure. Like Ding Ying’s taxonomic sys- tem of cultivated rice, the subspecific differentiation was the most distinct differentiation within culti- vated rice. But the differentiation within indica or japonica population was different: japonica presented clearer differentiation between soil-watery ecotypes than indica, and indica presented clearer differen- tiation between seasonal ecotypes than japonica. Cultivated rices in Guizhou revealed high genetic diversity at both DNA and phenotypic levels. Possessing the highest genetic diversity and all the nec- essary conditions as a center of genetic diversity, region Southwestern of Guizhou was suggested as the center of genetic diversity of O. sativa L. from Guizhou. | ZHANG DongLing ZHANG HongLiang WEI XingHua QI YongWen WANG Meixing SUN JunLi DING Li TANG ShengXiang QIU Zong'En CAO YongSheng WANG XiangKun LI ZiChao | 2007 | Chinese Science Bulletin2007，52，3： | 5 |
| 12 | High-performance quasi-2D perovskite light-emitting diodes: from materials to devices显示文摘Quasi-two-dimensional(quasi-2D)perovskites have attracted extraordinary attention due to their superior semiconducting properties and have emerged as one of the most promising materials for next-generation lightemitting diodes(LEDs).The outstanding optical properties originate from their structural characteristics.In particular,the inherent quantum-well structure endows them with a large exciton binding energy due to the strong dielectricand quantum-confinement effects;the corresponding energy transfer among different n-value species thus results in high photoluminescence quantum yields(PLQYs),particularly at low excitation intensities.The review herein presents an overview of the inherent properties of quasi-2D perovskite materials,the corresponding energy transfer and spectral tunability methodologies for thin films,as well as their application in high-performance LEDs.We then summarize the challenges and potential research directions towards developing high-performance and stable quasi-2D PeLEDs.The review thus provides a systematic and timely summary for the community to deepen the understanding of quasi-2D perovskite materials and resulting LED devices. | Li Zhang Changjiu Sun Tingwei He Yuanzhi Jiang Junli Wei Yanmin Huang Mingjian Yuan | 2021 | Light(Science & Applications)2021，10，4： | 5 |
| 13 | Joint frequency,2-D DOA,and polarization estimation using parallel factor analysis显示文摘This paper proposes a new algorithm for joint frequency, two-dimensional (2-D) directions-of-arrival (DOA), and polarization estimation using parallel factor (PARAFAC) analysis model and cumulant. The proposed algorithm designs a new array configuration, and extends the PARAFAC analysis model from the common data-domain and subspace-domain to the cumulant one, and forms three-way arrays by using the three cumulant matrices obtained from the properly chosen dipole outputs, and analyzes the uniqueness of low-rank decomposition of the three-way arrays, and then jointly estimates the source parameters via the low-rank decomposition of the constructed PARAFAC model. In comparison with the conventional methods, the proposed method alleviates the aperture loss, and avoids pairing parameter. Finally, the simulation results are presented to validate the performance of the proposed method. | LIANG JunLi LIU Ding ZHANG JunYing | 2009 | Science in China(Series F)2009，52，10： | 4 |
| 14 | Clinical value of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy in the treatment of advanced non-small cell lung cancer: a Meta-analysis显示文摘OBJECTIVE: To investigate the clinical efficacy and safety of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy in the treatment of advanced non-small celllung cancer(NCSLC) compared with chemotherapy alone.METHODS: Databases including Chinese National Knowledge Infrastructure, China Biology Medicine Disc, Wanfang, and MEDLINE were searched until April 1, 2014. Two assessors independently reviewed each trial. The primary outcome was the effective rate(ER) of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy. The secondary outcomes included quality of life improvement rate(QOLIR) and adverse reactions. Statistical calculations were performed by using Cochrane Collaboration Review Manager 5.2.RESULTS: A total of 888 patients from 15 studies,13 randomized controlled trials(RCT) and two controlled clinical trials, were included. Compared with chemotherapy alone, Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract plus chemotherapy significantly improved ER [Risk ratio(RR) =1.32, 95% CI,(1.14, 1.54)](based on 15 studies) and QOLIR [RR = 2.04, 95% CI,(1.69, 2.47)](based on 13studies). Compared with chemotherapy alone,Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract plus chemotherapy significantly inhibited chemotherapy-induced white blood cell decline [RR = 0.79, 95% CI,(0.70, 0.90)(based on 10 studies), chemotherapy-induced platelet decline[RR = 0.77, 95% CI,(0.60, 0.98)](based on 8 studies),and significantly alleviated nausea and vomiting(NV) [RR = 0.83, 95% CI,(0.71, 0.97)](based on 7studies). There was no significant difference in hemoglobin decline between the two therapies [RR =0.88, 95% CI,(0.70, 1.09)](based on 6 studies).CONCLUSION: This Meta-analysis suggests that Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy may be more efficacious in the treatment of advanced NSCLC than chemotherapy alone. This effect includes enhancing ER and QOLIR, and weakening chemotherapy toxicity. However, large-scale RCTs are required to further investigate the short- and long-term effects of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract. | Zhang Haiming Zhang Junli Ding Hao Chen Rui Liang Fengxia | 2016 | Journal of Traditional Chinese Medicine2016，36，3： | 4 |
| 15 | Downer reactor simulation and its application on coal pyrolysis:A review显示文摘Pyrolysis technology has received increasing attention in recent years due to its great potential in the field of lowrank coal clean and efficient conversion.Since pyrolysis reaction is very fast and prone to overreaction,the downer-type reactor is considered as a pyrolyzer due to its unique plug flow reactor characteristics.However,the low solids holdup,which is not beneficial for the fast heat transfer,limits its industrial application.Thus,how to realize high-density operation is crucial to the successful application of the downer reactor.Herein,the definition and strategies of high-density operation in the downer were introduced at first.And then,considering the increasing influence of computational fluid dynamics(CFD)in the fluidization industry,the state-of-the-art progress in downer simulation was reviewed,in which the newly developed drag models for downers were carefully discussed and compared.Also,to help prediction of the pyrolysis behaviors,the widely used pyrolysis kinetic models were systematically summarized.Combined with the potential of the downer in the field of coal pyrolysis,the relevant research progress of hot-state simulation of the downer pyrolyzer were introduced and analyzed.Finally,the suggestions on how to carry out follow-up work were given.It is expected that this review could give a better understanding for designing and optimizing downer pyrolyzer. | Xueer Pan Wenhao Lian Jingxuan Yang Junli Wang Zhonglin Zhang Xiaogang Hao Abuliti Abudula Guoqing Guan | 2022 | Carbon Resources Conversion2022，5，1： | 4 |
| 16 | Concise and efficient direct-view generation of arbitrary cylindrical vector beams by a vortex half-wave plate显示文摘A concise, efficient, and practical direct-view scheme is presented to generate arbitrary cylindrical vector(CV)beams, including CV beams, vortex beams, and cylindrical vector vortex(CVV) beams, by a vortex half-wave plate(VHP). Six kinds of first-order and other high-order CV beams, such as azimuthally polarized(AP) beams, antivortex radial polarization mode beams, and three-order AP beams, are formed by simply rotating a half-wave plate. The Stokes parameters and double-slit interference of multitype CV beams are investigated in detail.The polarization parameters, including degree of polarization, polarization azimuth, and ellipticity, are obtained,which demonstrates the efficient generation of CV beams. In addition, the double-slit interference experiment is introduced in the setup, and fringe misplacement and tilt appear for CVV beams, in which the misplacement number M is 2P+1 for P ≤ 2 and 2P-1 for P ≥ 3, where P is the polarization order number, and the fringe tilt offset is positively related to the topological charge number l of CVV beams. In addition, new types of VHPs can be formed by cascading two or more VHPs when the types of available VHPs are limited, assisting in more flexible generation of multitype CV beams. It is experimentally demonstrated that arbitrary CV beams with high quality are effectively achieved by the proposed setup, and the double-slit interference method can be utilized to determine and analyze CV beams rapidly and concisely by practical performance, which shows the potential to be implemented as a commercial device. | JUNLI QI WEIHUA WANG BO SHI HUI ZHANG YANAN SHEN HAIFEI DENG WENJING PU XIN LIU HUIHUI SHAN XIAOMIN MA LIANQIANG ZHANG WEI LU MEICHENG FU XIUJIAN LI | 2021 | Photonics Research2021，9，5： | 4 |
| 17 | Uptake of oxidative stress-mediated extracellular vesicles by vascular endothelial cells under low magnitude shear stress显示文摘Extracellular vesicles(EVs)are increasingly used as delivery vehicles for drugs and bioactive molecules,which usually require intravascular administration.The endothelial cells covering the inner surface of blood vessels are susceptible to the shear stress of blood flow.Few studies demonstrate the interplay of red blood cell-derived EVs(RBCEVs)and endothelial cells.Thus,the phagocytosis of EVs by vascular endothelial cells during blood flow needs to be elucidated.In this study,red blood cell-derived extracellular vesicles(RBCEVs)were constructed to investigate endothelial cell phagocytosis in vitro and animal models.Results showed that low magnitude shear stress including low shear stress(LSS)and oscillatory shear stress(OSS)could promote the uptake of RBCEVs by endothelial cells in vitro.In addition,in zebrafish and mouse models,RBCEVs tend to be internalized by endothelial cells under LSS or OSS.Moreover,RBCEVs are easily engulfed by endothelial cells in atherosclerotic plaques exposed to LSS or OSS.In terms of mechanism,oxidative stress induced by LSS is part of the reason for the increased uptake of endothelial cells.Overall,this study shows that vascular endothelial cells can easily engulf EVs in areas of low magnitude shear stress,which will provide a theoretical basis for the development and utilization of EVs-based nano-drug delivery systems in vivo. | Xian Qin Kun Zhang Juhui Qiu Nan Wang Kai Qu Yuliang Cui Junli Huang Li Luo Yuan Zhong Tian Tian Wei Wu Yi Wang Guixue Wang | 2022 | Bioactive Materials2022，7，3： | 3 |
| 18 | Extracellular calmodulin accelerates rbcS-GUS expression in suspension-cultured transgenic tobacco cell显示文摘The role of extracellular calmodulin in regulating expression of rbcS in darkness was examined. A suspension-cultured cell line was generated from the transgenic rbcS-GUS tobacco. It was demonstrated that purified calmodulin added to the media enhanced rbcS-GUS expression. The time course of expression of rbcS-GUS and that of the secretion of calmodulin in the suspended transgenic tobacco cells in darkness were very similar. Both showed initial increase followed by decline with maximum calmodulin secretion preceding maximum GUS expression. The addition of membrane-impermeable calmodulin antagonist W7-agarose inhibited the expression of rbcS-GUS in darkness, but this inhibitory effect was completely reversed by adding exogenous purified calmodulin. These results provide the first evidence that extracellular calmodulin accelerates rbcS gene expression. | MA Ligeng ZHOU Junli ZHANG Suqiao LIU Qiang SUN Daye | 2000 | Chinese Science Bulletin2000，45，22： | 3 |
| 19 | Experimental investigation of main controls to methane adsorption in clay-rich rocks显示文摘 | Liming Ji Tongwei Zhang Kitty L. Milliken Junli Qu Xiaolong Zhang | 2012 | Applied Geochemistry2012，，12： | 3 |
| 20 | Genetic structure and eco-geographical differentiation of cultivated Hsien rice(Oryza sativa L.subsp.indica) in China revealed by microsatellites显示文摘Indica is not only an important rice subspecies widely planted in Asia and the rest of the world,but it is also the genetic background of the majority of hybrid varieties in China.Studies on genetic structure and genetic diversity in indica germplasm resources are important for the classification and utilization of cultivated rice in China.Using a genetically representative core collection comprising 1482 Chinese indica landraces,we analysed the genetic structure,geographic differentiation and diversity.Model-based structure analysis of varieties within three ecotypes revealed nine eco-geographical types partially accordant with certain ecological zones in China.Differentiation of eco-geographical types was attributed to local ecological adaption and physical isolation.These groups may be useful for developing heterotic groups of indica.To facilitate the identification of different ecotypes and eco-geographical types,we identified characteristic SSR alleles of each ecotype and eco-geographical type and a rapid index of discrimination based on characteristic alleles.The characteristic alleles and rapid discrimination index may guide development of heterotic groups,and selection of hybrid parents. | ZHANG DongLing ZHANG HongLiang QI YongWen WANG MeiXing SUN JunLi DING Li LI ZiChao | 2013 | Chinese Science Bulletin2013，58，3： | 3 |
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