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| 1 | Screening for main components associated with the idiosyncratic hepatotoxicity of a tonic herb, Polygonum multiflorum显示文摘典型药用的植物的主要成分,蓼 multiflorum (用汉语的 Heshouwu ) ,那导致特质的肝损害仍然保持不清楚。我们的以前的工作证明了有 lipopolysaccharide (LPS ) 的无毒的剂量和 Heshouwu 的治疗学的剂量的 cotreatment 能在老鼠导致肝损害,而独唱治疗不能导致看得见的损害。在现在的工作,用成分大美人和敲门在里面策略,我们发现 Heshouwu 的乙醇醋酸盐(EA ) 摘录在对待 LPS 的老鼠显示了可比较的特质的 hepatotoxicity 到整个摘录。结果显示血浆丙氨酸 aminotransferase, aspartate aminotransferase,和肝 histologic 的重要举起变化,而另外的分开的部分没能导致肝损害。有另外的分开的部分的 EA 摘录的混合在对待 LPS 的老鼠导致了可比较的特质的 hepatotoxicity 到整个摘录。化学分析进一步揭示了那 2,3,5,4-tetrahydroxy trans-stilbene-2-O -- 配糖物(trans-SG ) 和它的 cis 异构体是在 EA 的二主要混合物摘录。而且,孤立的 cis- ,并且不它的 trans 异构体,显示的可比较的特质的 hepatotoxicity 到在对待 LPS 的老鼠的 EA 摘录。cis-SG 的更高的内容从与收集的将军相比与 Heshouwu 联系的实际的肝沉醉病人在 Heshouwu 白酒或准备被检测样品。另外,血浆 metabolomics 分析证明 cis-SG-disturbing 充实显著地不同于的小径在对待 LPS 的老鼠的 trans-SG。所有这些结果建议 cis-SG 仔细与 Heshouwu 的特质的 hepatotoxicity 被联系。认为 trans-SG 的 cis-trans 异构化被紫外光或日光调停,我们的调查结果为在药发现控制 photoisomerization 并且为 Heshouwu 和芪相关的药的临床的使用用作参考。 | Chunyu Li Ming Niu Zhaofang Bai Congen Zhang Yanling Zhao Ruiyu Li Can Tu Huifang Li Jing Jing Yakun Meng Zhijie Ma Wuwen Feng Jinfa Tang Yun Zhu Jinjie Li Xiaoya Shang Zhengsheng Zou Xiaohe Xiao Jiabo Wang | 2017 | Frontiers of Medicine2017，11，2： | 41 |
| 2 | Chronic Hepatitis B Infection with Low Level Viremia Correlates with the Progression of the Liver Disease显示文摘Background and Aims:Currently,insufficient clinical data are available to address whether low-level viremia(LLV)observed during antiviral treatment will adversely affect the clinical outcome or whether treatment strategies should be altered if LLV occurs.This study compared the clinical out-comes of patients with a maintained virological response(MVR)and patients who experienced LLV and their treatment strategies.Methods:A retrospective cohort of 674 patients with chronic hepatitis B virus(HBV)infection who received antiviral treatment for more than 12 months was analyzed for the development of end-stage liver disease and treatment strategies during the follow-up period.End-stage liver disease included decompensated liver cirrhosis and hepatocellular carcinoma(HCC).Results:During a median 42-month follow-up,end-stage liver disease developed more frequently in patients who experienced LLV than in those who experienced MVR(7.73%and 15.85%vs.0.77%and 5.52%at 5 and 10 years,respectively;p=0.000).The trend was consistent after propensity score matching.In the high-risk group of four HCC risk models,LLV patients had a higher risk of HCC development(p<0.05).By Cox proportional hazard model analysis,LLV was an independent risk factor for end-stage liver disease and HCC(hazard ratio[HR]=6.280,confidence interval[CI]=2.081-18.951,p=0.001;HR=5.108,CI=1.392-18.737,respectively;p=0.014).Patients achieved a lower rate of end-stage liver disease by adjusting treatment compared to continuing the original treatment once LLV occurred(p<0.05).Conclusions:LLV is an independent risk factor for end-stage liver disease and HCC,and treatment adjustments can be considered. | Qian Zhang Hong Peng Xiaoqing Liu Huimin Wang Jinjie Du Xinhua Luo Hong Ren Peng Hu | 2021 | Journal of Clinical and Translational Hepatology2021，9，6： | 25 |
| 3 | Genome Editing Enables Next-Generation Hybrid Seed Production Technology显示文摘The next-generation hybrid seed technology enables the successful production of sortable hybrid seeds from genic male sterile(GMS)lines and maintainers;however,it requires multiple laborious and complicated steps.Here,we designed a simple next-generation hybrid seed production strategy that takes advantage of the CRISPR/Cas9 technology to create a Manipulated GMS Maintainer(MGM)system via a single transformation.Under this schema,the maize male fertility gene ZmMS26 was nullified by removal of its fifth exon using the CRISPR/Cas9 system on a vector,and a second vector carrying a functional ZmMS26 cDNA was co-transformed to restore fertility.The second vector also contains a male gametophyte inactivation gene(ZmAA1)encoding maizeα-amylase driven by the pollen-specific promoter PG47 and an endosperm fluorescent marker(DsRED)driven by the barley endosperm aleurone-specific promoter Ltp2.The derived single-copy hemizygous MGM lines bore a mutated MS26 gene,leading to complete male sterility but normal vegetative growth and grain yield.The MGM system could prevent genetic transmission of the MGM elements via male gametophytes,providing an efficient method for sorting maintainer seeds labeled by DsRED.This strategy can be extended to any GMS gene and to hybrid crops other than maize. | Xiantao Qi Congsheng Zhang Jinjie Zhu Changlin Liu Changling Huang Xinhai Li Chuanxiao Xie | 2020 | Molecular Plant2020，13，9： | 18 |
| 4 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 5 | Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9显示文摘CRISPR/Cas(clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins) is an adaptive immune system in bacteria and archaea to defend against invasion from foreign DNA fragments. Recently, it has been developed as a powerful targeted genome editing tool for a wide variety of species. However, its application in maize has only been tested with transiently expressed somatic cells or with a limited number of stable transgenic T0 plants. The exact efficiency and specificity of the CRISPR/Cas system in the highly complex maize genome has not been documented yet. Here we report an extensive study of the well-studied type II CRISPR-Cas9 system for targeted genome editing in maize, with the codon-optimized Cas9 protein and the short non-coding guide RNA generated through a functional maize U6 sn RNA promoter. Targeted gene mutagenesis was detected for 90 loci by maize protoplast assay, with an average cleavage efficiency of 10.67%. Stable knockout transformants for maize phytoene synthase gene(PSY1) were obtained. Mutations occurred in germ cells can be stably inherited to the next generation. Moreover, no off-target effect was detected at the computationally predicted putative off-target loci. No significant difference between the transcriptomes of the Cas9 expressed and non-expressed lines was detected. Our results confirmed that the CRISPR-Cas9 could be successfully applied as a robust targeted genome editing system in maize. | Jinjie Zhu Ning Song Silong Sun Weilong Yang Haiming Zhao Weibin Song Jinsheng Lai | 2016 | Journal of Genetics and Genomics2016，43，1： | 12 |
| 6 | Experimental Investigation on Vibration Control of Rotor-bearing System with Active Magnetic Exciter显示文摘Vibration control is an efficient way to minimize a rotating machine's vibration level so that its vibration fault-free can be realized.While,several factors,such as unbalance,misalignment and instability,contribute to the serious vibration of rotating machines.It is necessary that one apparatus can depress vibration caused by two or more reasons.The fault self-recovery(FSR) mechanism is introduced and investigated.Strategies of vibration control are investigated theoretically using numerical method firstly.Active magneticelectric exciter(AME) are selected as the actuator of a FSR device because it can provide suitable force by varying the control current in the exciters depending upon a proportional and derivative control law.By numerical study,it is indicate that only a small control force is needed to improve stability margins of the compressor and prevent subsynchronous vibration fault efficiently.About synchronous vibration,three control strategies,searching in whole circle,fast optimizing control(FOC),and none mistaking control,are investigated to show which of the control strategy can realize the fault self-recovery in the shortest time.Experimental study is conducted on a test rig with variable rotating speed.Results of the test indicate that the non-mistake control strategy can minimize synchronous vibration in less than three seconds.The proposed research can provide a new insight for subsynchronous and synchronous vibration restraining about centrifugal compressor. | WANG Weimin GAO Jinji HUANG Liquan XIN Zhengqiu | 2011 | Chinese Journal of Mechanical Engineering2011，24，6： | 11 |
| 7 | Precise base editing of non-allelic acetolactate synthase genes confers sulfonylurea herbicide resistance in maize显示文摘Single-nucleotide polymorphisms contribute to phenotypic diversity in maize. Creation and functional annotation of point mutations has been limited by the low efficiency of conventional methods based on random mutation. An efficient tool for generating targeted single-base mutations is desirable for both functional genomics and precise genetic improvement. The objective of this study was to test the efficiency of targeted C-to-T base editing of two non-allelic acetolactate synthase(ALS) in generating sulfonylurea herbicide-resistant mutants. A CRISPR/Cas9 nickase-cytidine deaminase fused with uracil DNA glycosylase inhibitor(UGI) was employed to achieve targeted conversion of cytosine to thymine in ZmALS1 and ZmALS2. Both protoplasts and recovered mutant plants showed the activity of the cytosine base editor, with an in vivo efficiency of up to 13.8%. Transgene-free edited plants harboring a homozygous ZmALS1 mutation or a ZmALS1 and ZmALS2 double mutation were tested for their resistance at a dose of up to 15-fold the recommended limit of chlorsulfuron, a sulfonylurea herbicide widely used in agriculture. Targeted base editing of C-to-T per se and a phenotype verified in the generated mutants demonstrates the power of base editing in precise maize breeding. | Yanmin Li Jinjie Zhu Hao Wu Changlin Liu Changling Huang Jinhao Lan Yanming Zhao Chuanxiao Xie | 2020 | The Crop Journal2020，8，3： | 9 |
| 8 | The possible contribution of a mantle-derived fluid to the Ningwu porphyry iron deposits—Evidence from carbon and strontium isotopes of apatites显示文摘Apatite is a characteristic mineral for the iron deposits in the Ningwu region. Apatite shows a range of d13CPDB values from à9.6& to à0.6&,and most of them with the d13CPDB values distributed in a range of mantle carbon (i.e.,à2& to à9&),except for samples T-06-1 and J-06-3. Apatites in the Ningwu porphyry iron deposits yield a narrow variation range of 87Sr/86Sr values from 0.706326 to 0.707577,similar to those of the volcanic and subvolcanic rocks and higher than that of a typical mantle-derived magmatic apatite (i.e.,about 0.7040),indicating that formation of the apatites was involved with crust material contamination. d13CPDB and 87Sr/86Sr values of the apatite indicate a possible contribution of mantle-derived fluid and material to formation of the Ningwu porphyry iron deposits,supported by regional geology,regional gravities and geochemistry of the volcanic and subvolcanic rocks in the Ningwu region. | Jinjie Yu Jingwen Mao Changqing Zhang | 2008 | Progress in Natural Science:Materials International2008，18，2： | 7 |
| 9 | A Review on Self-Recovery Regulation(SR)Technique for Unbalance Vibration of High-End Equipment显示文摘The high-end equipment represented by high-end machine tools and aero-engines is the core component of the national intelligent manufacturing plan,and the mass unbalance is the main reason for its excessive vibration,that seriously impacts the operation efficiency and running life of the equipment.In order to change the traditional way that the fault of equipment can only be repaired by human,the self-recovery mechanism of human and animal are given to the equipment in this paper,which forms the self-recovery regulation(SR)system for unbalance vibration of high-end equipment.The system can online generate the self-recovery force to restrain the unbalance vibration of the equipment in operation,which is an important direction for the development of the equipment to the advanced intelligent stage.Based on the basic principles of SR technique,the typical engineering application cases of this technique in the field of aeroengine and high-end machine tools are introduced,and four related studies promoting the development of this technique are summarized and analyzed in turn.It includes feature extraction,imbalance location,regulation method and balancing actuator.Self-recovery Regulation(SR)Technique is an important way to realize intelligent manufacturing and intelligent maintenance.Relevant research can lay a technical foundation for the development of high-end equipment with self-health function. | Xin Pan Jiaqiao Lu Jiaji Huo Jinji Gao Haiqi Wu | 2020 | Chinese Journal of Mechanical Engineering2020，33，6： | 7 |
| 10 | Jerk-limited feedrate scheduling and optimization for five-axis machining using new piecewise linear programming approach显示文摘In this paper, a new computation method and an optimization algorithm are presented for feedrate scheduling of five-axis machining in compliance with both machine drive limits and process limits. Five-axis machine tool with its ability of controlling tool orientation to follow the sculptured surface contour has been widely used in modern manufacturing industry. Feedrate scheduling serving as a kernel of CNC control system plays a critical role to ensure the required machining accuracy and reliability for five-axis machining. Due to the nonlinear coupling effects of all involved drive axes and the saturation limit of servo motors, the feedrate scheduling for multi-axis machining has long been recognized and remains as a critical challenge for achieving five-axis machine tools’ full capacity and advantage. To solve the nonlinearity nature of the five-axis feedrate scheduling problems, a relaxation mathematical process is presented for relaxing both the drive motors’ physical limitations and the kinematic constraints of five-axis tool motions. Based on the primary optimization variable of feedrate, the presented method analytically linearizes the machining-related constraints, in terms of the machines’ axis velocities, axis accelerations and axis jerks. The nonlinear multi-constrained feedrate scheduling problem is transformed into a manageable linear programming problem. An optimization algorithm is presented to find the optimal feedrate scheduling solution for the five-axis machining problems. Both computer implementation and laboratorial experiment testing by actual machine cutting were conducted and presented in this paper. The experiment results demonstrate that the proposed method can effectively generate efficient feedrate scheduling for five-axis machining with constraints of the machine tool physical constraints and limits. Compared with other existing numerical methods, the proposed method is able to find an accurate analytical solution for the nonlinear constrained five-axis feedrate scheduling problems without compromising the efficiency of the machining processes. | SUN YuWen CHEN ManSen JIA JinJie LEE Yuan-Shin GUO DongMing | 2019 | Science China(Technological Sciences)2019，62，7： | 7 |
| 11 | Study on hydrostatic gas lubricated non-contacting mechanical seal显示文摘 | 李双喜 Zhu Liang Cai Jining Zhang Qiuxiang Gao Jinji | 2012 | High Technology Letters2012，18，4： | 6 |
| 12 | GNP6,a novel allele of MOC1,regulates panicle and tiller development in rice显示文摘The yield of rice is mostly affected by three factors,namely,panicle number,grain number and grain weight.Variation in panicle and grain numbers is mainly caused by tiller and panicle branches generated from axillary meristems(AMs).MOC1 encodes a putative GRAS family nuclear protein that regulates AM formation.Although several alleles of MOC1 have been identified,its variation in germplasm resources remains unclear.In the present study we characterized a novel mocl allele named gnp6 which has a thymine insertion in the coding sequence of the SAW motif in the GRAS domain.This mutation causes arrested branch formation.The SAW motif is necessary for nuclear localization of GNP6/MOC1 where it functions as a transcription factor or co-regulator.Haplotype analysis showed that the coding region of GNP6/MOC1 was conserved without any non-synonymous mutations in 240 rice accessions.However,variation in the promoter region might affect the expression of it and its downstream genes.Joint haplotype analysis of GNP6/MOC1 and MOC3 showed that haplotype combinations H9,H10 and H11,namely MOC1-Hap1 in combination with MOC3-Hap3,MOC3-Hap4 or MOC3-Hap5 could be bred to promote branch formation.These findings will enrich the genetic resources available for rice breeders. | Zhanying Zhang Xingming Sun Xiaoqian Ma Bingxia Xu Yong Zhao Zhiqi Ma Gangling Li Najeeb Ullah Khan Yinghua Pan Yuntao Liang Hongliang Zhang Jinjie Li Zichao Li | 2021 | The Crop Journal2021，9，1： | 6 |
| 13 | Geochemistry of Apatite from the Apatite-rich Iron Deposits in the Ningwu Region,East Central China显示文摘磷灰石的四种类型在 Ningwu 区域被识别了。磷灰石的第一种类型广泛地在中间的黑暗有颜色的地区被散布(即铁矿) 单个存款。集合包括磁铁矿,磷灰石和阳起石(或透辉石) 。Thesecond 类型在铁矿发生在磁铁矿磷灰石静脉以内。第三种类型是看见的 inmagnetite 磷灰石静脉并且(或) 在主人岩石中的小瘤(即斑粝岩闪长岩斑岩 orgabbro 闪长岩或辉石闪长岩) 。第四种类型在在 Xiangshan 组充满破裂的 apatite-pyrite-quartz 静脉以内发生。在斑岩铁矿床的四出现的磷灰石的稀土元素元素(REE ) 地球化学被介绍。磷灰石的 REE 分发模式通常类似于在 Kiruna 类型铁矿的磷灰石的那些, nelsonites。他们在轻 REE 被充实,与显著否定 Eu 异例。在从在在世界上的不同地点的各种各样的存款的磷灰石的 REE 分发模式的类似为各种各样的矿石类型显示形成的一个普通过程,例如 immiscibility。早 magmatic 磷灰石 contain3031.48-12080 x 10 ^(-6) REE。后来热水的磷灰石包含 1958 x10^(-6) REE,显示以后热水的形成矿石的答案包含降低 REE。尽管斑粝岩闪长岩斑岩和磷灰石显示出类似的 REE 模式,斑粝岩闪长岩斑岩没有铕异例或微弱的积极或微弱的否定的铕异例,在一个高氧易逃逸条件下面由披风源地的减小环境并且由分别和结晶化(immiscibility ) 引起了。磷灰石的否定 Eu 异例被更早的 diopsite 在矿石岩浆冷却期间由于 Eu^(2+) 的获得可能形成。在 Aoshan 和泰山铁矿床的磷灰石产出Sr/^( 86 ) Sr 从 0.7071~0.7073 珍视的^( 87 )的一个狭窄的变化范围,类似于那些暴烈并且潜水艇火山岩,显示磷灰石被液体 immiscibility 和中间、基本的岩浆的区别形成。 | YU Jinjie ZHANG Qi MAO Jingwen YAN Shenghao | 2007 | Acta Geologica Sinica(English Edition)2007，81，4： | 5 |
| 14 | Bio-Based Hyperbranched Toughener From Tannic Acid and Its Enhanced Solvent-Free Epoxy Resin with High Performance显示文摘It is essential to design economic and efficient tougheners to prepare high-performance epoxy resin;however,this has remained a huge challenge.Herein,an eco-friendly,low-cost,and facile-fabricated bio-based hyperbranched toughener,carboxylic acid-functionalized tannic acid(CATA),was successfully prepared and applicated to the preparation of solvent-free epoxy resins.The mechanical performance,morphology,structural characterization,and thermal characterization of toughened epoxy resin system were studied.The toughened epoxy resin system with only 1.0wt%CATA reached the highest impact strength,111%higher than the neat epoxy resin system.Notably,the tensile strength and elongation at break of toughened epoxy resin systems increased moderately with increasing CATA loading.Nonphase-separated hybrids with significant toughening effect were obtained.Additionally,the thermal stabilities of toughened epoxy resin systems decreased with increasing CATA loading.This study provides an eco-friendly,cost-effective,and facile approach for the preparation of high-performance,solvent-free epoxy resins with potential for practical applications in sealing integrated circuits and electrical devices fields. | Jie Xu Jiayao Yang Hengxu Wang Peng Lin Xiaohuan Liu Jinjie Zhang Shenyuan Fu Yuxun Tang | 2019 | Journal of Renewable Materials2019，7，12： | 5 |
| 15 | Evaluation of Comprehensive Benefits of Land Consolidation Based on AHP and FUZZY显示文摘Based on the modern comprehensive evaluation method, in accordance with the scientific, objective and systematic principles, we select four factors (economic, social, ecological and landscape benefits), to establish the evaluation indicator system of comprehensive benefits of rural land consolidation. Then using the theory of fuzzy mathematics (FUZZY) and fuzzy relationship synthesis theory, we establish the fuzzy evaluation model of comprehensive benefits of land consolidation; using analytic hierarchy process (AHP), we determine the weight of each indicator, and conduct fuzzy comprehensive evaluation from various levels. By the weighted average synthesis algorithm, we calculate the evaluation indicators and finally summarize the overall evaluation results for comprehensive evaluation. Taking the case of land consolidation project in Shangyao Town, Datong District, Huainan City in Anhui Province, we calculate the case data based on Matlab software, and the calculation results and evaluation level are consistent with the project acceptance results, verifying the feasibility of this evaluation method. | Jinsong TIAN Jinjie TIAN Jiachun GUO | 2013 | Asian Agricultural Research2013，5，1： | 5 |
| 16 | Thermal–structure coupling analysis and multi-objective optimization of motor rotor in MSPMSM显示文摘With the high speed, the rotor of magnetically suspended permanent magnet synchronous motor(MSPMSM) suffers great thermal stress and mechanical stress resulting from the temperature rise problem caused by rotor losses, which leads to instability and inefficiency.In this paper, the mechanical–temperature field coupling analysis is conducted to analyze the relationship between the temperature field and structure, and multi-objective optimization of a rotor is performed to improve the design reliability and efficiency. Firstly, the temperature field is calculated by the 2 D finite element model of MSPMSM and the method of applying the 2 D temperature result to the 3 D finite element model of the motor rotor equivalently is proposed. Then the thermal–structure coupling analysis is processed through mathematic method and finite element method(FEM),in which the 3 D finite element model is established precisely in a way and approaches the practical operation state further. Moreover, the impact produced by the temperature and structure on the mechanical strength is analyzed in detail. Finally, the optimization mathematical model of the motor rotor is established with Sequential Quadratic Programming-NLPQL selected in the optimization scheme. Through optimization, the strength of the components in the motor rotor increases obviously and satisfies the design requirement, which to a great extend enhances the service life of the MSPMSM rotor. | Luxin ZHAI Jinji SUN Xin MA Weitao HAN Xiaosan LUO | 2019 | Chinese Journal of Aeronautics2019，32，7： | 5 |
| 17 | Conversion of a normal maize hybrid into a waxy version using in vivo CRISPR/Cas9 targeted mutation activity显示文摘Waxy maize is a specialty maize that produces mainly amylopectin starch with special food or industrial values. The objective of this study was to overcome the limitations of wx mutant allele acquisition and breeding efficiency by conversion of parental lines from normal to waxy maize. The intended mutation activity was achieved by in vivo CRISPR/Cas9 machinery involving desired-target mutation of the Wx locus in the ZC01 background,abbreviated as ZC01-DTM^(wx). Triple selection was applied to segregants to obtain high genome background recovery with transgene-free wx mutations. The targeted mutation was identified, yielding six types of mutations among progeny crossed with ZC01-DTM^(wx).The amylopectin contents of the endosperm starch in mutant lines and hybrids averaged94.9%, while those of the wild-type controls were significantly(P < 0.01) lower, with an average of 76.9%. Double selection in transgene-free lines was applied using the Bar strip test and Cas9 PCR screening. The genome background recovery ratios of the lines were determined using genome-wide SNP data. That of lines used as male parents was as high as98.19% and that of lines used as female parents was as high as 86.78%. Conversion hybrids and both parental lines showed agronomic performance similar to that of their wild-type counterparts. This study provides a practical example of the efficient extension of CRISPR/Cas9 targeted mutation to industrial hybrids for transformation of a recalcitrant species. | Xiantao Qi Hao Wu Haiyang Jiang Jinjie Zhu Changling Huang Xin Zhang Changlin Liu Beijiu Cheng | 2020 | The Crop Journal2020，8，3： | 4 |
| 18 | Fine mapping of the awn gene on chromosome 4 in rice by association and linkage analyses显示文摘Awnness is a key trait in rice domestication, yet no studies have been conducted on fine mapping or association mapping of the rice awn gene. In this study, we investigated the awnness and genotype of a core collection of 303 cultivated rice varieties and a BC5F2 segregating population of 200 individuals. Combining association and linkage analyses, we mapped the awnness related genes to chromosome 4. Primary association analysis using 24 SSR markers revealed five loci significantly associated with awnness on chromosome 4. The associated markers cover previously identified regions. Fine association mapping was conducted using another 29 markers within a 4-Mb region, covering the associated marker in34, which is close to the awn gene Awn4.1. Seven associated markers were revealed, distributed over an 870-kb region. Combining the fine association mapping and linkage analysis of awnness in the 200 BC5F2 segregating population, we finally identified a 330-kb region as the candidate region for Awn4.1. The results indicate that combining association mapping and linkage mapping provides an efficient and precise approach to both genome-wide mapping and fine mapping of rice genes. | HU GuangLong ZHANG DongLing PAN HuiQiao LI Ben WU JianTao ZHOU XueYa ZHANG QunYuan ZHOU Lei YAO GuoXin LI JunZhou LI JinJie ZHANG HongLiang LI ZiChao | 2011 | Chinese Science Bulletin2011，56，9： | 4 |
| 19 | Comparative transcriptional analysis provides insights of possible molecular mechanisms of wing polyphenism induced by postnatal crowding in Aphis gossypii显示文摘Background:Aphis gossypii is a worldwide sap-sucking pest with a variety of hosts and a vector of more than 50 plant viruses.The strategy of wing polyphenism,mostly resulting from population density increasing,contributes to the evolutionary success of this pest.However,the related molecular basis remains unclear.Here,we identified the effects of postnatal crowding on wing morph determination in cotton aphid,and examined the transcriptomic differences between wingless and wing morphs.Results:Effect of postnatal crowding on wing determination in A gossypii was evaluated firstly.Under the density of 5 nymphs·cm-2,no wing aphids appeared.Proportion of wing morphs rised with the increase of density in a certain extent,and peaked to 56.1% at the density of 20 nymphs·cm-2,and reduced afterwards.Then,transcriptomes of wingless and wing morphs were assembled and annotated separately to identify potentially exclusively or differentially expressed transcripts between these two morphs,in which 3 126 and 3 392 unigenes annotated in Nr(Non-redundant protein sequence) database were found in wingless or wing morphs exclusively.Moreover,3 187 up-and 1 880 down-regulated genes were identified in wing versus wingless aphid.Pathways analysis suggested the involvement of differentially expressed genes in multiple cellular signaling pathways involved in wing morphs determination,including lipid catabolic and metabolism,insulin,ecdysone and juvenile hormone biosynthesis.The expression levels of related genes were validated by the reverse transcription quantitative real time polymerase chain reaction(RT-qPCR) soon afterwards.Conclusions:The present study identified the effects of postnatal crowding on wing morphs induction and demonstrated that the critical population density for wing morphs formation in A gossypii was 20 nymphs·cm-2.Comparative transcriptome analysis provides transcripts potentially expressed exclusively in wingless or wing morph,respectively.Differentially expressed genes between wingless and wing morphs were identified and several signaling pathways potentially involved in cotton aphid wing differentiation were obtained. | JI Jichao ZHANG Shuai LUO Junyu WANG Li ZHU Xiangzhen ZHANG Kaixin ZHANG Lijuan CUI Jinjie | 2019 | Journal of Cotton Research2019，2，3： | 3 |
| 20 | Research on fault diagnosis method of piston rod based on harmonic wavelet and manifold learning显示文摘As the core part of reciprocating compressor,piston rod is easy to cause a serious accident when abrasion and breakage fault occur to it. Therefore,it is very important to monitor its running state. At present,a small number of reciprocating compressors have been installed on-line monitoring and diagnosis system,most of which can only monitor a single vertical subsidence of piston rod and it can't fully represent the running state of piston rod. Therefore,a method of monitoring the vertical and horizontal displacement of piston rod axis orbit is simultaneously used. In view of the characteristics that the piston rod axis orbit is disordered and difficult to extract features,purification of the axis orbit is carried out based on harmonic wavelet and then features are extracted such as vibration energy,natural frequency and the axis orbit envelope area. After that,a nonlinear local tangent space manifold learning algorithm is used to reduce the dimension of the features and obtain sensitive features. By analyzing the practical cases,the effectiveness of the method for fault monitoring and diagnosis of reciprocating compressor piston rod assembly has been verified. Finally,as BP neural network has the characteristics of solving complex nonlinear problems,the validity of the fault diagnosis method of reciprocating compressor piston rod based on harmonic wavelet and manifold learning is proved by actual case data analysis based on BP neural network. | 江志农 Zhu Lina Zhang Jinjie Zhou Chao | 2018 | High Technology Letters2018，24，3： | 3 |
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