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| 1 | Transcriptomics-based identification of WRKY genes and characterization of a salt and hormone-responsive PgWRKY1 gene in Panax ginseng显示文摘WRKY 蛋白质属于一个抄写因素(TF ) 家庭和戏在许多植物过程,对不能生活、关於生命的压力的包括的植物回答,以及第二等的新陈代谢的动态角色。然而,在人参属人参 C ·迈耶的 WRKY 基因都没迄今为止被报导。在这研究,从甲基 jasmonate (MeJA ) 的很多 WRKY unigenes 对待偶然这种的根 transcriptome 用下一代的定序被识别技术。编码 WRKY 蛋白质的 48 有希望的 WRKY unigenes 的一个总数被消除冤屈和不完全的开的读物框架(ORF ) 获得。种系发生的分析揭示 48 WRKY TF,包括 11 组我, 36 组 II,和 1 组 III。而且,指定为 PgWRKY1 的一 MeJA 应答的 unigene 被克隆并且描绘。它包含 1077 bp 的全部 ORF 并且编码 358 氨基酸残余的多肽。PgWRKY1 蛋白质包含由保存氨基酸顺序主题 WRKYGQK 和 C 2 H 2-type 锌手指主题属于 WRKY 亚群 II-d。在洋葱和烟草外皮房间的 PgWRKY1-GFP 熔化蛋白质的 Subcellular 本地化表明 PgWRKY1 在原子核是只在场的。量的即时聚合酶链反应分析证明 PgWRKY1 的表示与叶子,茎,和种子相比在根和侧面的根是相对更高的。重要地, PgWRKY1 表示被水杨酸显著地导致酸, abscisic 酸,和 NaCl,而是由 MeJA 处理的 downregulated。这些结果建议 PgWRKY1 可能是对荷尔蒙和盐压力作出回应的多重压力可诱导的基因。 | Mohammed Nuruzzaman Hongzhe Cao Hao Xiu Tiao Luo Jijia Li Xianghui Chen Junli Luo Zhiyong Luo | 2016 | Acta Biochimica et Biophysica Sinica2016，48，2： | 12 |
| 2 | Ginsenoside Rh2 inhibits proliferation and induces apoptosis in human leukemia cells via TNF-~ signaling pathway显示文摘人参皂甙 Rh2,从人参属人参提取的 triterpene saponin,对多重癌症的展览药理学活动。然而,人参皂甙 Rh2 的 anticancer 机制是不清楚的。在这研究,我们发现人参皂甙 Rh2 有效地禁止生长并且导致 HL-60 房间的 apoptosis。用 microarray 技术,我们发现了那个肿瘤坏死因素 --(TNF-) 清楚地是起来调整的。而且, anti-TNF- 抗体经由 caspase-8, caspase-9,和 caspase-3 激活的抑制减轻了导致 Rh2 的 HL-60 房间 apoptosis。另外, TNF- 起来规定也在另外的对待 Rh2 的癌症房间线被观察。这些结果证明 TNF- 在人参皂甙起一个关键作用导致 Rh2 的房间 apoptosis。 | Jingjia Huang Kunjian Peng Linghao Wang Bin Wen Lin Zhou Tiao Luo Min Su Jijia Li Zhiyong Luo | 2016 | Acta Biochimica et Biophysica Sinica2016，48，8： | 11 |
| 3 | Transcriptomics-based identification and characterization of 11 CYP450 genes of Panax ginseng responsive to MeJA显示文摘Cytochromes P450 (CYP450s),a superfamily of mono-oxygenases,are essential to generate highly functionalized secondary metabolites in plants and contribute to the diversification of specialized triterpenoid biosynthesis in eudicots.However,screening and identifying the exact CYP450 genes in ginsenoside biosynthesis is extremely challenging due to existence of large quantities of members in CYP450 superfamily.Therefore,to screen the CYP450 genes involved in ginsenoside bio- synthesis,transcriptome dataset of Panax ginseng was created in our previous work using the technique of the next-generation sequencing.On the basis of bioinformatics analysis,16 putative CYP450 genes with significant differential expression were screened from the dataset and submit- ted to GenBank,in which 11 of them have been cloned.Methyl jasmonate (MeJA)was used as an elicitor to analyze the expression profiles of candidate CYP450 genes by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR).The results of qRT-PCR analysis revealed that the expression of some CYP450 genes were strongly induced by MeJA and showed different transcription levels at different treatment time points.Homology analysis indicated that each putative CYP450 protein of P.ginseng has a conserved domain consisting of E-E-R-F-P-R-G. The CYP450 genes were screened and cloned here to enrich the resources of CYP450 genes,and the results of bioinformatics analysis provided a foundation to further identify the function of CYP450s involved in ginsenoside biosynthesis.Furthermore,this study facilitated the construction of microbial cell factories for increasing the production of ginsenosides by means of metabolic engineering. | Xu Zeng Tiao Luo Jijia Li Gui Li Donghua Zhou Tuo Liu Xian Zou Aparna Pandey Zhiyong Luo | 2018 | Acta Biochimica et Biophysica Sinica2018，50，11： | 2 |
| 4 | Selective TBK1/IKKi dual inhibitors with anticancer potency显示文摘 | Jijia Li Jingjia Huang Ji‐Hak Jeong Sun‐Jin Park Rui Wei Jieying Peng Zhiyong Luo Yen Ting Chen Yangbo Feng Jun‐Li Luo | 2014 | Int. J. Cancer2014，，： | 1 |
| 5 | Ginsenoside Rh2 inhibits breast cancer cell growth via ERβ-TNFα pathway显示文摘Ginsenoside Rh2 is one of rare panaxidiols extracted from Panax ginseng and a potential estrogen receptor ligand that exhibits moderate estrogenic activity.However,the effect of Rh2 on growth inhibition and its underlying molecular mechanism in human breast cells are not fully understood.In this study,we tested cell viability by MTT and colony formation assays.Cell growth and cell cycle were determined to investigate the effect of ginsenoside Rh2 by flow cytometry.The expressions of estrogen receptors(ERs),TNFα,and apoptosis-related proteins were detected by qPCR and western blot analysis.The mechanisms of ERαand ERβaction were determined using transfection and inhibitors.Antitumor effect of ginsenoside Rh2 against MCF-7 cells was investigated in xenograft mice.Our results showed that ginsenoside Rh2 induced apoptosis and G1/S phase arrest in MCF-7 cells.Treatment of cells with ginsenoside Rh2 down-regulated protein levels of ERα,and up-regulated mRNA and protein levels of ERβand TNFα.We also found that ginsenoside Rh2-induced TNFαover-expression is through up-regulation of ERβinitiated by ginsenoside Rh2.Furthermore,ginsenoside Rh2 induced MCF-7 cell apoptosis via estrogen receptorβTNFαpathway in vivo.These results demonstrate that ginsenoside Rh2 promotes TNFα-induced apoptosis and G1/S phase arrest via regulation of ERβ. | Kunjian Peng Tiao Luo Jijia Li Jingjia Huang Zizeng Dong Jia Liu Chaoqiong Pi Zizeng Zou Qin Gu Ousheng Liu Jian-Ting Zhang Zhi-Yong Luo | 2022 | Acta Biochimica et Biophysica Sinica2022，54，5： | 1 |
| 6 | DYNAMIC BUCKLING OF STATICALLY PRELOADED RING-STIFFENED CYLINDRICAL SHELLS UNDER AXIAL FLUID-SOLID IMPACT LOADING显示文摘The Initial Imperfection Amplified Criterion is applied to investigate the geometric nonlineardynamic buckling of statically preloaded ring-stiffened cylindrical shells under axial fluid-solid impact.Tak-ing account of the effects of large deformation and initial geometric imperfection,the governing equations areobtained by the Galerkin method and solved by the Runge-Kutta method.The effects of static preloading(uniform external radial pressure)on the buckling features and the load-carrying ability of ring-stiffened cy-lindrical shells against axial impact are discussed. | Jiang Songqing Li Yongchi Hu Xiuzhang Zheng Jijia | 2000 | Acta Mechanica Solida Sinica2000，13，3： | 1 |
| 7 | Identification and phylogenetic analysis of Vibrio vulnificus isolated from diseased Trachinotus ovatus in cage mariculture显示文摘 | Guifeng Li Dianhui Zhao Lu Huang Jijia Sun Dong Gao Haifang Wang Yongli Tan Lanqing Liang | 2006 | Aquaculture2006，，1： | 1 |
| 8 | RNA modification in cardiovascular disease: implications for therapeutic interventions显示文摘Cardiovascular disease(CVD)is the leading cause of death in the world,with a high incidence and a youth-oriented tendency.RNA modification is ubiquitous and indispensable in cell,maintaining cell homeostasis and function by dynamically regulating gene expression.Accumulating evidence has revealed the role of aberrant gene expression in CVD caused by dysregulated RNA modification.In this review,we focus on nine common RNA modifications:N^(6)-methyladenosine(m^(6)A),N^(1)-methyladenosine(m^(1)A),5-methylcytosine(m^(5)C),N^(7)-methylguanosine(m^(7)G),N^(4)-acetylcytosine(ac^(4)C),pseudouridine(Ψ),uridylation,adenosine-to-inosine(A-to-I)RNA editing,and modifications of U34 on tRNA wobble.We summarize the key regulators of RNA modification and their effects on gene expression,such as RNA splicing,maturation,transport,stability,and translation.Then,based on the classification of CVD,the mechanisms by which the disease occurs and progresses through RNA modifications are discussed.Potential therapeutic strategies,such as gene therapy,are reviewed based on these mechanisms.Herein,some of the CVD(such as stroke and peripheral vascular disease)are not included due to the limited availability of literature.Finally,the prospective applications and challenges of RNA modification in CVD are discussed for the purpose of facilitating clinical translation.Moreover,we look forward to more studies exploring the mechanisms and roles of RNA modification in CVD in the future,as there are substantial uncultivated areas to be explored. | Cong Wang Xuyang Hou Qing Guan Huiling Zhou Li Zhou Lijun Liu Jijia Liu Feng Li Wei Li Haidan Liu | 2023 | Signal Transduction and Targeted Therapy2023，8，11： | 0 |
| 9 | Effect of bastnaesite as reductant on hematite reduction during in-situ suspension magnetization roasting of refractory iron ore under neutral atmosphere显示文摘The iron tailings of Bayan Obo mines are solid waste,which occupies land area and also causes environmental pollution;however,this waste can be recycled.In this study,based on the characteristics of iron minerals and fluorocarbonate contained in Bayan Obo iron tailings,clean magnetization roasting of iron minerals by bastnaesite from iron tailings during in-situ suspension magnetization roasting in a neutral atmosphere was explored.The results show that for iron tailings with a mass of 12 g,a N_(2) gas flow rate of 600 mL/min,and roasting for 5 min at 800℃,iron concentrate with a 60.44%iron grade at an iron recovery of 76.04%could be obtained.X-ray diffraction analysis showed that the weak magnetic hematite was reduced to strong magnetic magnetite in the neutral atmosphere,without additional reductant.The kinetics of the magnetization roasting of mineral mixtures(bastnaesite and hematite)in a neutral atmosphere showed that the optimal reaction mechanism function was the three-dimensional diffusion model with activation energy of 161.8838 kJ·mol^(-1);this indicates that the reaction was a heterogeneous,diffusion-controlled solid-state reaction. | Wenbo Li Jijia Chen Wentao Zhou Yuexin Han Yan Shan | 2022 | International Journal of Mining Science and Technology2022，32，4： | 0 |
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