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| 1 | Screening efficiency and screen length of a linear vibrating screen using DEM 3D simulation显示文摘The effect of screen length on the screening efficiency of particles is studied under various single parameter conditions including frequency, amplitude, vibration angle, and screen inclination. The Discrete Element Method (DEM) has been used to simulate the screening process. A functional relationship between screening efficiency and screen length is established. It is shown that screening efficiency and screen length have a complicated exponential relationship. Relationships between them are profoundly discussed and conclusions are easily drawn: low values of the parameters do not benefit screening; screening efficiency generally increases with screen length; screening efficiency reaches a plateau when these parameters are in range frequently encountered in practical applications. | Wang Guifeng Tong Xin | 2011 | Mining Science and Technology2011，21，3： | 19 |
| 2 | Study on the physiological indices of Pinus sibirica and Pinus koraiensis seedlings under cold stress显示文摘Although Pinus sibirica and Pinus koraiensis are resistant to cold or low temperatures in the cold temperate regions of the northern hemisphere,the former has a stronger cold resistance.Research has been limited to the comparison of physiological responses of the two species to cold stress.In this study,5-year-old seedlings of P.sibirica and P.koraiensis were subjected to six temperature treatments,[20℃(control),0℃,-20℃,-40℃,-60℃,and-80℃],under different stress periods(6,12,24,and 48 h).The results showed that differences in each physiological index were significant between P.sibirica and P.koraiensis,except for the permeability of cell membranes,reactive oxygen species,proline and soluble proteins.An ANOVA test indicated that there were extreme differences among the temperatures for each index,stress time and temperature 9 time for most indices.All indices showed a similar trend for P.sibirica and P.koraiensis with decreasing temperature or the extension of stress time.Soluble sugars and proline increased at 0 to-20℃and then remained unchanged with temperature decline.Other indices showed an increase from 20 to-20℃,stable from-20 to-40℃and a decrease from-40 to-80℃.All the indices increased and then declined along with the prolonged cold stress time,except for the control.From 0 to-40℃,the permeability of cell membranes,relative conductivity,reactive oxygen species and malonaldehyde of P.koraiensis seedlings were higher than in P.sibirica,but superoxide dismutase,peroxidase,catalase activity and soluble sugars,proline,and soluble proteins content emerged as opposite.This study compared the physiological mechanism responses to cold stress between P.sibirica and P.koraiensis to provide the basis for the introduction,distribution,and genetic improvement of these coniferous species. | Fang Wang Deyang Liang Xiaona Pei Qinhui Zhang Peng Zhang Jianqiu Zhang Zhimin Lu Yuchun Yang Guifeng Liu Xiyang Zhao | 2019 | Journal of Forestry Research2019，30，4： | 12 |
| 3 | Development of a modified Mooney-Rivlin constitutive model for rubber to investigate the effects of aging and marine corrosion on seismic isolated bearings显示文摘In this study,aging and marine corrosion tests of a large number of rubber material and rubber bearings have been carried out.The constitutive Mooney-Rivlin model parameters for a rubber isolated bearing have been determined.By applying the least-square method to the experimental data,the relationships between the aging time and the marine corrosion time with the constants in the constitutive model for a rubber bearing have been derived.Next,the Mooney-Rivlin model has been modified accordingly.Further,using the modified Mooney-Rivlin model and the Abaqus software,the performance of the rubber isolated bearings has been simulated.The simulation results have been compared to the experimental results so as to verify the accuracy of the modified model.The comparison shows that the maximum errors for the vertical and horizontal stiffnesses are 16.8%and 0.49%,respectively.Since these errors are considered acceptable,the accuracy of the modified constitutive model can be considered verified.The results of this study can provide theoretical support for the performance study on rubber isolated bearings under the complex ocean environment and the life-cycle performance evaluation of bridges and other offshore structures. | Zhao Guifeng Ma Yuhong Li Yanmin Luo Jiarun Du Chang | 2017 | Earthquake Engineering and Engineering Vibration2017，16，4： | 8 |
| 4 | Differentiation of illicit drugs with THz time-domain spectroscopy显示文摘The terahertz time-domain spectroscopy(THz-TDS) was used for sensing and identifying illicit drugs.The absorption spectra of seven illicit drug samples(morphine and its hydrochloride,cocaine hydrochloride,codeine phosphate,papaverine hydrochloride,pethidine hydrochloride,and thebaine) were studied by THz-TDS at 0.3-2.0 THz at room temperature.The geometric structure and vibration frequencies of morphine were calculated by density functional theory.The four absorption features were dominated by intra-/inter-molecular collective or lattice vibration modes.Each illicit drug has a distinct signature in its THz spectra.The results indicate that the THz-TDS can be used to identify and discriminate illicit drugs by their characteristic fingerprints. | LIU Guifeng MA Shihua JI Te ZHAO Hongwei WANG Wenfeng | 2010 | Nuclear Science and Techniques2010，21，4： | 7 |
| 5 | Olfactory ensheathing cell transplantation improves sympathetic skin responses in chronic spinal cord injury显示文摘Forty-three patients with chronic spinal cord injury for over 6 months were transplanted with embryonic olfactory ensheathing cells, 2–4 × 106, into multiple sites in the injured area under the surgical microscope. The sympathetic skin response in patients was measured with an electromyography/evoked potential instrument 1 day before transplantation and 3–8 weeks after transplantation.Spinal nerve function of patients was assessed using the American Spinal Injury Association impairment scale. The sympathetic skin response was elicited in 32 cases before olfactory ensheathing cell transplantation, while it was observed in 34 cases after transplantation. Concomitantly,sympathetic skin response latency decreased significantly and amplitude increased significantly after transplantation. Transplantation of olfactory ensheathing cells also improved American Spinal Injury Association scores for movement, pain and light touch. Our findings indicate that olfactory ensheathing cell transplantation improves motor, sensory and autonomic nerve functions in patients with chronic spinal cord injury. | Zuncheng Zheng Guifeng Liu Yuexia Chen Shugang Wei | 2013 | Neural Regeneration Research2013，8，30： | 6 |
| 6 | Development and utilization of a new chemically-induced soybean library with a high mutation density显示文摘Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate(EMS)-induced soybean(Glycine max) population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_(4(5))families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll(Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene(ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean. | Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu | 2017 | Journal of Integrative Plant Biology2017，59，1： | 6 |
| 7 | Genetic variation and selection of introduced provenances of Siberian Pine(Pinus sibirica) in frigid regions of the Greater Xing'an Range,Northeast China显示文摘Siberian Pine(Pinus sibirica) is an ecologically and economically important species in pristine forests throughout northern Russia. Four provenances of P. sibirica were introduced from Mongolia and Russia to the Greater Xing'an Range(the Daxing'anling), northeast China in 1993. The aim of this research was to study genetic variation and selection of the introduced four Pinus sibirica provenances. Heights(H), basal diameters(BD), survival rates(SR) and crown lengths(CL) of different families were measured as primary outcomes in different growth years. Results of data analyses demonstrated high coefficients of phenotypic variation(PCV) and heritability(H2) for H, BD and CL at 18 years after introduction. PCV and H2 increased with age. Correlations of growth traits between any two growth years were all significantly positive, but the correlation coefficient was smaller when the growth year interval was larger. Correlations between H and the original environment factors decreased gradually, indicating that with long-term subsistence in the new environment, the influence of the source environment declined. Colligation of multiple traits to estimate provenances showed that Novosibirsk, Tomsk, and Altai Mountains had higher survival rates and biomass, and proved more suitable for introduction and plantation in the Greater Xing'an Range in China. | Xiyang Zhao Cheng Wang Shuchun Li Wei Hou Shiqing Zhang Guijie Han Deng Pan Ping Wang Yufei Cheng Guifeng Liu | 2014 | Journal of Forestry Research2014，25，3： | 6 |
| 8 | Long-term anodal block stimulation at sacral anterior roots promoted recovery of neurogenic bladder function in a rabbit model of complete spinal cord injury显示文摘A complete spinal cord injury model was established in experimental rabbits using the spinal cord clip compression method.Urodynamic examination was performed 2 weeks later to determine neurogenic bladder status.The rabbits were treated with anodal block stimulation at sacral anterior roots for 4 weeks.Electrical stimulation of sacral anterior roots improved urodynamic parameters of neurogenic bladder in rabbit models of complete spinal cord injury,effectively promoted urinary function,and relieved urinary retention.Immunohistochemistry results showed that a balance was achieved among expression of muscarinic receptor subunits M2,M3,ATP-gated ion channel P2X3 receptors,and β2-adrenergic receptor,and nerve growth factor expression decreased.These results suggested that long-term sacral anterior root stimulation of anodal block could be used to treat neurogenic bladder in a rabbit model of complete spinal cord injury. | Xiaoran Wang Qi Gao Xiaoyu Yang Weihua Wang Xinquan Gu Guifeng Liu Peng Yan Ge Gao Xin Yu Yongjie Wang Jihu Lian Chaoling Shi Yao Wang Li Fan | 2012 | Neural Regeneration Research2012，7，5： | 6 |
| 9 | Progeny test of tetraploid Betula platyphylla and preliminary selection of hybrid parents显示文摘Estimating genetic parameters of parental lines through progeny testing and choosing good hybrid parents are important for genetically improving seed orchard trees.In this study, 24 tetraploid progeny seedlings were used as experimental materials, which came from test cross design:six tetraploid Betula platyphylla lines(Q33, Q13, Q103,Q19, Q83 and Q14) as female parents and four individual B. platyphylla diploid lines(F3, F4, F9 and F11) as male parents were crossed. Variance analysis of height, diameter, height-to-diameter ratio, and internodal distance showed that the differences between hybrid combinations reached highly significant levels. Using multi-objective decisionmaking, we performed a comprehensive assessment of the various hybrid combinations. Using a selection rate of 20 % of the standard, five hybrids were selected;their genetic gains in average height, diameter, height-todiameter ratio, and internodal distance were 20.95, 6.07,13.07 and 8.96 %, respectively. We also analyzed the combining ability and genetic parameter effect values of parents and hybrid combinations. The combined analysis revealed that Q13, Q103, Q33 and Q83 were superior females; F3, F4, and F9 were superior males; and F3 × Q13, F4 × Q83 and F9 × Q33 were superior hybrid combinations. The heights and diameters of these progenies were 22.49 and 11.48 % greater than average,respectively. | Huanwen Xu Yu Liu Jing Jiang Guifeng Liu Xiyang Zhao | 2016 | Journal of Forestry Research2016，27，3： | 5 |
| 10 | Post-marketing safety surveillance and reevaluation of Motherwort injection:A clinical study of 10094 cases显示文摘OBJECTIVE:To investigate the safety profiles of Motherwort injection(MI).METHODS:A multi-center,prospective and drugderived hospital intensive monitoring method was conducted to assess the safety of MI in real world applications.This study was based on a very large population after the injection was approved and marketed in China.All patients using the injection in participating hospitals were monitored to determine the incidence,pattern,severity and outcome of associated adverse events.RESULTS:The post-marketing surveillance was performed in 10 094 female patients from April to December,2015.The incidence of adverse drug reactions(ADRs) was 0.79‰(8/10 094).Among the 8 patients,the reported adverse events mainly included systemic abnormalities,such as fever,chills and eyelid edema;skin and appendages disorders,such as pruritus and rash;gastrointestinal disorders,such as nausea,abdominal distension and pain;heart rate and rhythm disorders,such as palpitation and increased heart rate.All of these ADRs were mild in severity.CONCLUSION:In this study the ADRs incidence rate of MI is very low,which supports that it is generally safe for use in obstetric and gynecological diseases.However,the total number of 8 ADRs recorded over a relatively short time span seems limited,and the low number of reports could not represent an absolute guarantee of safety. | Cao Shan Zhang Wenhao Zhao Ziwei Heng Mingli Bu Huaien Wang Hongwu Liu Xinghui Wang Zhong Cai Yan Ma Yuyan Cui Shihong Deng Jihong Ding Guifeng Ding Yajuan Dong Linhong Duan Zhentao Fan Ling Fan Yang Fu Fen He Jing Ji Shuying Jin Lin Li Hong Li Hongying Liao Tao Lu Wei Luo Xiucui Lü Zhihui Ma Fengchun Ma Dafeng Shi Tianyun Sun Juying Sun Xiaotong Teng Hong Wang Jinhua Wang Ruihua Wang Ying Wang Zhengling Xi Jie Xu Minjuan Xu Zhihong Yan Qian Yang Cuirong Yang Yimei Yin Jie Yu Jinhua Yuan Wenjun Zhang Guanli Zhang Meihua Zhao Renfeng Zhong Yonghong Zhou Jian | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 4 |
| 11 | BpMADS12 gene role in lignin biosynthesis of Betula platyphylla Suk by transcriptome analysis显示文摘MADS-box transcription factors show highly diverse regulatory functions in a wide variety of organisms.In this study,we characterized a MADS-box gene(BpMADS12) from the white birch(Betula platyphylla Suk).This gene is a member of the suppressor of overexpression of CO 1/tomato MADS 3 class of MADS-box genes.We generated lines overexpressing BpMADS12 and found that these had higher levels of lignin compared to that observed in nontransgenic lines.Transcriptome analysis revealed numerous changes in gene expression patterns.In total,8794 differentially expressed genes were identified,including 5006 upregulated unigenes and 3788 downregulated unigenes in Bp MADS-overexpression lines.Differentially expressed genes involved in the pathways for lignin and brassinosteroid biosynthesis were significantly enriched and may have contributed to phenotypic changes.The results from a quantitative RT-PCR analysis were consistent those obtained with the transcriptome analysis.Our transcriptome analysis,in combination with measurement of lignin level,indicated that Bp MADS12 promotes lignin synthesis through regulation of key enzymes in response to brassinosteroid signaling.These results suggest that this MADS-box protein is crucial to all subsequent structural events and provide a good foundation for studies aiming to elucidate the developmental mechanisms underlying formation of wood. | Huiyu Li Yang Yang Zijia Wang Xiaohong Guo Feifei Liu Jing Jiang Guifeng Liu | 2016 | Journal of Forestry Research2016，27，5： | 4 |
| 12 | Analysis of three types of triterpenoids in tetraploid white birches(Betula platyphylla Suk.) and selection of plus trees显示文摘Betulin, oleanolic acid, and betulinic acid are naturally occurring pentacyclic triterpenoids that have significant medicinal value. Considerable amounts of these triterpenoids are available in the outer bark of white birch.In this study, we used ultrasound-assisted extraction(UAE)to extract triterpenoids from birch bark rapidly and with high efficiency. Using high performance liquid chromatography(HPLC), three types of triterpenoids were separated and detected. We examined the differences among triterpenoids extracted from diploid versus tetraploid white birch. Then, we used factor analysis to screen out tetraploid white birches with comprehensively excellent performance. The results indicate that the optimum conditions for extraction include the use of ethanol as an extraction solvent, a solid-to-liquid ratio of 0.1 g/10 ml,ultrasonic power set at 100 W, a temperature of 60 °C and an extraction time of 15 min. A reversed-phase C18 column(4.6 mm × 250 mm × 5 lm) with a column temperature of 30 °C and the mobile phase composed of A(acetonitrile) and B(0.1 % aqueous phosphoric acid, v/v)at a flow rate of 0.5 ml/min were used, and the detection wavelength was 195 nm. No significant difference was observed between diploid and tetraploid white birch in terms of the content of three types of triterpenoids(at a confidence level of 0.05). As triterpenoid content, height,and DBH(diameter at breast height) are strongly interrelated, we used factor analysis to evaluate all individuals,and we screened out six plus trees with excellent comprehensive characters. | Sui Wang Hui Zhao Jing Jiang Guifeng Liu Chuanping Yang | 2015 | Journal of Forestry Research2015，26，3： | 4 |
| 13 | Activated and deactivated functional brain areas in the Deqi state: A functional MRI study显示文摘We compared the activities of functional regions of the brain in the Deqi versus non-Deqi state, as reported by physicians and subjects during acupuncture. Twelve healthy volunteers received sham and true needling at the Waiguan (TE5) acupoint. Real-time cerebral functional MRI showed that compared with non-sensation after sham needling, true needling activated Brodmann areas 3, 6, 8, 9, 10, 11, 13, 20, 21, 37, 39, 40, 43, and 47, the head of the caudate nucleus, the parahippocampal gyrus, thalamus and red nucleus. True needling also deactivated Brodmann areas 1, 2, 3, 4, 5, 6, 7, 9, 10, 18, 24, 31, 40 and 46. | Yong Huang Tongjun Zeng Guifeng Zhang Ganlong Li Na Lu Xinsheng Lai Yangjia Lu Jiarong Chen | 2012 | Neural Regeneration Research2012，7，30： | 4 |
| 14 | Differential protein expression in spinal cord tissue of a rabbit model of spinal cordischemia/reperfusion injury显示文摘New Zealand rabbits were randomly divided into an ischemia group(occlusion of the abdominal aorta for 60 minutes),an ischemia-reperfusion group(occlusion of the abdominal aorta for 60 minutes followed by 48 hours of reperfusion) and a sham-surgery group.Two-dimensional gel electrophoresis detected 49 differentially expressed proteins in spinal cord tissue from the ischemia and ischemia/reperfusion groups and 23 of them were identified by mass spectrometry.In the ischemia group,the expression of eight proteins was up regulated,and that of the remaining four proteins was down regulated.In the ischemia/reperfusion group,the expression of four proteins was up regulated,and that of two proteins was down regulated.In the sham-surgery group,only one protein was detected.In the ischemia and ischemia/reperfusion groups,four proteins overlapped between groups with the same differential expression,including three that were up regulated and one down regulated.These proteins were related to energy metabolism,cell defense,inflammatory mechanism and cell signaling. | Qi Gao Yonghui Liang Xiaoyu Yang Guifeng Liu Xiaoxue Li Benqing Zhu Jian Liu Maoguang Yang Weiwei Xia Jian Dong Jianhang Jiao | 2012 | Neural Regeneration Research2012，7，20： | 4 |
| 15 | Comparative evaluation of the physicochemical properties of nano-hydroxyapatite/collagen and natural bone ceramic/collagen scaffolds and their osteogenesis-promoting effect on MC3T3-E1 cells显示文摘The use of various types of calcium phosphate has been reported in the preparation of repairing materials for bone defects.However,the physicochemical and biological properties among them might be vastly different.In this study,we prepared two types of calcium phosphates,nano-hydroxyapatite(nHA)and natural bone ceramic(NBC),into 3D scaffolds by mixing with type I collagen(CoL),resulting in the nHA/CoL and NBC/CoL scaffolds.We then evaluated and compared the physicochemical and biological properties of these two calcium phosphates and their composite scaffold with CoL.Scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS),Fourier-transform infrared spectroscopy(FTIR),X-ray diffraction spectroscopy(XRD)and compressive tests were used to,respectively,characterize the morphology,composition,distribution and the effect of nHA and NBC to collagen.Next,we examined the biological properties of the scaffolds using cytotoxicity testing,flow cytometry,immunofluorescence staining,biocompatibility testing,CCK-8 assays and RT-PCR.The results reflected that the Ca2t released from nHA and NBC could bind chemically with collagen and affect its physicochemical properties,including the infrared absorption spectrum and compression modulus,among others.Furthermore,the two kinds of scaffolds could promote the expression of osteo-relative genes,but showed different gene induction properties.In short,NBC/CoL could promote the expression of early osteogenic genes,while nHA/CoL could upregulate late osteogenic genes.Conclusively,these two composite scaffolds could provide MC3T3-E1 cells with a biomimetic surface for adhesion,proliferation and the formation of mineralized extracellular matrices.Moreover,nHA/CoL and NBC/CoL had different effects on the period and extent ofMC3T3-E1 cell mineralization. | Xiongxin Lei Jianping Gao Fangyu Xing Yang Zhang Ye Ma Guifeng Zhang | 2019 | Regenerative Biomaterials2019，6，6： | 4 |
| 16 | Optimization and evaluation of fish oil rnicrocapsules显示文摘 | Hui Liu Lia nyan Wang Tingyuan Yang Guifeng Zhang Jian Huang Jing Sun Junsheng Huo | 2016 | Particuology2016，14，6： | 4 |
| 17 | Specific cerebral activation following true and sham Waiguan(SJ 5) needling Functional magnetic resonance imaging evidence显示文摘Positive activation and negative activation of glucose metabolism can be detected in different brain regions under acupuncture conditions using functional magnetic resonance imaging(fMRI) which reveals acupoint function and location-acupoint specificity.This study observed the regional cerebral activation of the Waiguan(SJ 5) acupoint following true needling at the true Waiguan acupoint,sham needling at the true Waiguan acupoint and true needling at a sham point using fMRI.The fMRI results demonstrated that compared with sham needling,true needling at Waiguan activated the BA8 area and left cerebellum.Compared with needling at the sham point,needling at Waiguan activated the BA2 area,the left cerebellum and the right inferior semilunar lobule.Thus the activated brain areas caused by needling Waiguan may be the basis for treatment of certain diseases. | Yong Huang Xinsheng Lai Baoci Shan Chunzhi Tang Junjun Yang Tongjun Zeng Guifeng Zhang Jiarong Chen Yangjia Lu | 2010 | Neural Regeneration Research2010，5，22： | 3 |
| 18 | Expression of BpPIN is associated with IAA levels and the formation of lobed leaves in Betula pendula ’Dalecartica’显示文摘Auxin polar transport genes PIN(PINFORMED)determine the concentration gradient of auxin in plants.To understand the relationship between the development of different tissues in Betula pendula‘Dalecartica’,BpPIN gene expression and indole-3-acetic acid(IAA)content were analyzed using qRT-PCR,ELISA,and GUS staining.Gene expression of BpPIN genes and IAA levels in the leaves,buds,stems,xylem,and roots of B.pendula‘Dalecartica’and B.pendula as a control were measured.BpPIN1,BpPIN5 and BpPIN6 were upregulated during development in both species,suggesting a dominant role in the development of B.pendula‘Dalecartica’leaves.Moreover,BpPIN1 gene expression was positively associated with IAA levels during leaf,vein and petiole development in B.pendula‘Dalecartica’only.The correlation coefficient of the first three leaves was 0.69(P=0.04),while that of the first three petioles was 0.85(P=0.001).In addition,GUS staining of the pro-DR5::GUS transgenic line of cultivar was correlated with the results of BpPIN1 expression.Overall,these findings suggest that BpPIN1 is associated with the formation of lobed leaves in B.pendula‘Dalecartica’. | Chang Qu Xiuyan Bian Rui Han Jing Jiang Qibin Yu Guifeng Liu | 2020 | Journal of Forestry Research2020，31，1： | 3 |
| 19 | LOWER TEMPERATURE 1 Enhances ABA Responses and Plant Drought Tolerance by Modulating the Stability and Localization of C2-Domain ABA-Related Proteins in Arabidopsis显示文摘Plasma membrane-associated abscisic acid(ABA)signal transduction is an integral part of ABA signaling.The C2-domain ABA-related(CAR)proteins play important roles in the recruitment of ABA receptors to the plasma membrane to facilitate ABA signaling.However,how CAR proteins are regulated remains unclear.In this study,we conducted a genetic screen for mutants with altered leaf transpiration and identified an uncharacterized protein,LOWER TEMPERATURE 1(LOT1),which regulates the dynamic localization and stability of CAR proteins.The lotimutant had a lower leaf temperature as compared with the wild type due to higher transpiration.We found that LOT1 physically interacts with CAR9,and ABA reduces LOT1-CAR9 interaction in the nucleus,likely via Ca^2+,resulting in increased localization of CAR9 to the plasma membrane.We further found that the stability of CAR9 is affected by LOT1 less CAR9 proteins were accumulated and more were ubiquitinated in lot1.While the lot1 car9 and/of f car9 mutants were hyposerisitive to ABA,the hyposensitive phenotype of loticould be rescued by CAR9 overexpression.Collectively,our study reveals that LOT1 regulates plant tolerance to drought stress by affecting ABA signaling through regulating the stability and dynamic localization of CAR9. | Tao Qin Qiuzhen Tian Guifeng Wang Liming Xiong | 2019 | Molecular Plant2019，12，9： | 2 |
| 20 | Light-induced protein translocation by genetically encoded unnatural amino acid in Caenorhabditis elegans显示文摘Dear Editor,Living systems such as Caenorhabditis elegans are organized by interaction networks of biopolymer and small mol-ecules.The molecular engineering of specifi c targets to monitor and control these biological molecules has led to significant advances in our understand-ing of how biological systems are organ-ized,maintain themselves and disin-tegrate(Prescher and Bertozzi,2005).For instance,green fl uorescent protein(GFP)derived from jellyfi sh Aequorea Victoria helps us to accurately locate and observe proteins over long term periods.In addition,many novel LOV(light,oxy-gen,or voltage)domains such as LOV2-Jαand Vivid offer the possibility of cell-based motility management and tissue-based gene expression control(Wu et al.,2009;Wang et al.,2012). | Hao Chang Mei Han Wenming Huang Guifeng Wei Juanjuan Chen Peng R.Chen Runsheng Chen Junlong Zhang Tao Xu Pingyong Xu | 2013 | Protein & Cell2013，4，12： | 2 |
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