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| 1 | 3D printing of bone tissue engineering scaffolds显示文摘Tissue engineering is promising in realizing successful treatments of human body tissue loss that current methods cannot treat well or achieve satisfactory clinical outcomes.In scaffold-based bone tissue engineering,a high performance scaffold underpins the success of a bone tissue engineering strategy and a major direction in the field is to produce bone tissue engineering scaffolds with desirable shape,structural,physical,chemical and biological features for enhanced biological performance and for regenerating complex bone tissues.Three-dimensional(3D)printing can produce customized scaffolds that are highly desirable for bone tissue engineering.The enormous interest in 3D printing and 3D printed objects by the science,engineering and medical communities has led to various developments of the 3D printing technology and wide investigations of 3D printed products in many industries,including biomedical engineering,over the past decade.It is now possible to create novel bone tissue engineering scaffolds with customized shape,architecture,favorable macro-micro structure,wettability,mechanical strength and cellular responses.This article provides a concise review of recent advances in the R&D of 3D printing of bone tissue engineering scaffolds.It also presents our philosophy and research in the designing and fabrication of bone tissue engineering scaffolds through 3D printing. | Chong Wang Wei Huang Yu Zhou Libing He Zhi He Ziling Chen Xiao He Shuo Tian Jiaming Liao Bingheng Lu Yen Wei Min Wang | 2020 | Bioactive Materials2020，5，1： | 20 |
| 2 | Wnt and BMP signaling crosstalk in regulating dental stem cells:Implications in dental tissue engineering显示文摘Tooth is a complex hard tissue organ and consists of multiple cell types that are regulated by important signaling pathways such as Wnt and BMP signaling.Serious injuries and/or loss of tooth or periodontal tissues may significantly impact aesthetic appearance,essential oral functions and the quality of life.Regenerative dentistry holds great promise in treating oral/dental disorders.The past decade has witnessed a rapid expansion of our understanding of the biological features of dental stem cells,along with the signaling mechanisms governing stem cell self-renewal and differentiation.In this review,we first summarize the biological characteristics of seven types of dental stem cells,including dental pulp stem cells,stem cells from apical papilla,stem cells from human exfoliated deciduous teeth,dental follicle precursor cells,periodontal ligament stem cells,alveolar bone-derived mesenchymal stem cells(MSCs),and MSCs from gingiva.We then focus on how these stem cells are regulated by bone morphogenetic protein(BMP)and/or Wnt signaling by examining the interplays between these pathways.Lastly,we analyze the current status of dental tissue engineering strategies that utilize oral/dental stem cells by harnessing the interplays between BMP and Wnt pathways.We also highlight the challenges that must be addressed before the dental stem cells may reach any clinical applications.Thus,we can expect to witness significant progresses to be made in regenerative dentistry in the coming decade. | Fugui Zhang Jinlin Song Hongmei Zhang Enyi Huang Dongzhe Song Viktor Tollemar Jing Wang Jinhua Wang Maryam Mohammed Qiang Wei Jiaming Fan Junyi Liao Yulong Zou Feng Liu Xue Hu Xiangyang Qu Liqun Chen Xinyi Yu Hue H.Luu Michael J.Lee Tong-Chuan He Ping Ji | 2016 | Genes & Diseases2016，3，4： | 16 |
| 3 | Molecular Cloning and Bioinformatics Analysis of TypeⅢSecretion System Effector Protein Va1686 Gene of Vibrio alginolyticus显示文摘In this study,Va1686 gene was cloned from Vibrio alginolyticus. The total length of the gene is 1 164 bp,and it could encode 387 amino acids. The physicochemical properties,protein structure,genetic evolutionary relationship and antigenic characteristics of the effect protein Va1686 of V. alginolyticus HY9901 type Ⅲ secretion system were studied and analyzed by bioinformatics methods and tools. The results showed that Va1686 is a stable hydrophilic and acidic protein without a transmembrane region and a signal peptide,and secondary structure to α-helix. The evolutionary analysis showed that V. alginolyticus HY9901 and V.harveyi were clustered together,which indicated that the genetic relationship between the two species was the closest. Va1686 contains a Fic superfamily conserved domain associated with cell division. Bioinformatics analysis showed that the B-cell preponderant epitopes of Va1686 might be localized in the regions of 48-49,82-85,125-126,150-153,185-186,236-237 and so on. The 3D structure model of Va1686 subunit was simulated by SWISS-MODEL software and it was found that the vop S of V. parahaemolyticus was similar and the similarity was 89. 46%. In this study,the feasibility of Va1686 as a common antigen of Vibrio was verified from the perspective of bioinformatics,which laid the foundation for the next step in vaccine development. | Zhihao WU Mingjie FAN Jiaming LIAO Huanying PANG Chuanhao PAN Dawei SONG Mingsheng QIU Shuanghu CAI Jichang JIAN | 2018 | Agricultural Biotechnology2018，7，5： | 2 |
| 4 | Long noncoding RNA(lncRNA)H19:An essential developmental regulator with expanding roles in cancer,stem cell differentiation,and metabolic diseases显示文摘Recent advances in deep sequencing technologies have revealed that,while less than 2%of the human genome is transcribed into mRNA for protein synthesis,over 80%of the genome is transcribed,leading to the production of large amounts of noncoding RNAs(ncRNAs).It has been shown that ncRNAs,especially long non-coding RNAs(lncRNAs),may play crucial regulatory roles in gene expression.As one of the first isolated and reported lncRNAs,H19 has gained much attention due to its essential roles in regulating many physiological and/or pathological processes including embryogenesis,development,tumorigenesis,osteogen-esis,and metabolism.Mechanistically,H19 mediates diverse regulatory functions by serving as competing endogenous RNAs(CeRNAs),Igf2/H19 imprinted tandem gene,modular scaffold,cooperating with H19 antisense,and acting directly with other mRNAs or lncRNAs.Here,we summarized the current understanding of H19 in embryogenesis and development,cancer development and progression,mesenchymal stem cell lineage-specific differentiation,and metabolic diseases.We discussed the potential regulatory mechanisms underlying H19’s func-tions in those processes although more in-depth studies are warranted to delineate the exact molecular,cellular,epigenetic,and genomic regulatory mechanisms underlying the physiolog-ical and pathological roles of H19.Ultimately,these lines of investigation may lead to the development of novel therapeutics for human diseases by exploiting H19 functions. | Junyi Liao Bowen Chen Zhenglin Zhu Chengcheng Du Shengqiang Gao Guozhi Zhao Piao Zhao Yonghui Wang Annie Wang Zander Schwartz Lily Song Jeffrey Hong William Wagstaff Rex CHaydon Hue HLuu Jiaming Fan Russell RReid Tong-Chuan He Lewis Shi Ning Hu Wei Huang | 2023 | Genes & Diseases2023，10，4： | 1 |
| 5 | Feasible strategies for studying the involvement of DNA methylation and histone acetylation in the stress-induced formation of quality-related metabolites in tea(Camellia sinensis)显示文摘Tea plants are subjected to multiple stresses during growth,development,and postharvest processing,which affects levels of secondary metabolites in leaves and influences tea functional properties and quality.Most studies on secondary metabolism in tea have focused on gene,protein,and metabolite levels,whereas upstream regulatory mechanisms remain unclear.In this review,we exemplify DNA methylation and histone acetylation,summarize the important regulatory effects that epigenetic modifications have on plant secondary metabolism,and discuss feasible research strategies to elucidate the underlying specific epigenetic mechanisms of secondary metabolism regulation in tea.This information will help researchers investigate the epigenetic regulation of secondary metabolism in tea,providing key epigenetic data that can be used for future tea genetic breeding. | Jie Yang Dachuan Gu Shuhua Wu Xiaochen Zhou Jiaming Chen Yinyin Liao Lanting Zeng Ziyin Yang | 2021 | Horticulture Research2021，8，1： | 1 |
| 6 | Enhanced recovery of high-purity Fe powder from iron-rich electrolytic manganese residue by slurry electrolysis显示文摘Iron-rich electrolytic manganese residue(IREMR)is an industrial waste produced during the processing of electrolytic metal manganese,and it contains certain amounts of Fe and Mn resources and other heavy metals.In this study,the slurry electrolysis technique was used to recover high-purity Fe powder from IREMR.The effects of IREMR and H2SO4 mass ratio,current density,reaction temper-ature,and electrolytic time on the leaching and current efficiencies of Fe were studied.According to the results,high-purity Fe powder can be recovered from the cathode plate,and the slurry electrolyte can be recycled.The leaching efficiency,current efficiency,and purity of Fe reached 92.58%,80.65%,and 98.72wt%,respectively,at a 1:2.5 mass ratio of H2SO4 and IREMR,reaction temperature of 60℃,electric current density of 30 mA/cm^(2),and reaction time of 8 h.In addition,vibrating sample magnetometer(VSM)analysis showed that the coercivity of electrolytic iron powder was 54.5 A/m,which reached the advanced magnetic grade of electrical pure-iron powder(DT4A coercivity standard).The slurry electrolytic method provides fundamental support for the industrial application of Fe resource recovery in IRMER. | Wenxing Cao Jiancheng Shu Jiaming Chen Zihan Li Songshan Zhou Shushu Liao Mengjun Chen Yong Yang | 2024 | International Journal of Minerals,Metallurgy and Materials2024，31，3： | 0 |
| 7 | Cloning and Bioinformatics Analysis of tye A Gene of Vibrio alginolyticus显示文摘[Objectives]This study aimed to clone the tye A gene of Vibrio alginolyticus HY9901 strain and analyze its sequence by bioinformatics.[Methods] By referring to the entire genome sequence of Ⅴ.alginolyticus on Gen Bank,specific primers were designed.According to the principle of PCR amplification,the target gene tye A was amplified.By means of bioinformatics,the sequence of tye A was further analyzed,and the phylogenetic tree of tye A genes of Vibrio spp.and the corresponding subunit three-dimensional structure models were constructed.[Results] The length of the tye A gene of Ⅴ.alginolyticus strain HY9901 is 285 bp,and its theoretical molecular weight is 10.98 kD.According to prediction,there is no signal peptide or transmembrane region at the N-terminus of the sequence,and the amino acid sequence contains two casein kinase Ⅱ phosphorylation sites.The results of protein subcellular localization prediction show that the Tye A protein is located in the cell membrane.The protein is unstable and non-hydrophilic.The tertiary structure of Tye A protein of Ⅴ.alginolyticus is similar to that of Yersinia sp.According to prediction,Tye A has a major functional domain Pfam.In terms of secondary structure,alpha helix,random coil,extended strand and beta turn account for 85.11%,7.45%,4.26% and 3.19%,respectively.The homology of Tye A between Ⅴ.alginolyticus and Vibrio parahaemolyticus is up to 83%,so they are classified into one cluster.[Conclusions]This study will help to further understand the regulation mechanism of type Ⅲ secretion system in Ⅴ.alginolyticus. | Jiaming LIAO Weijie ZHANG Chunhui XU Kaishan LIANG Shihui ZHOU Huanying PANG Miao XIE | 2019 | Asian Agricultural Research2019，11，10： | 0 |
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