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| 1 | Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection显示文摘Hepatitis delta virus(HDV) seems to strongly suppress hepatitis B virus(HBV)replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein(HBx) is essential for HBV replication, determination of HBV X gene(HBX)quasispecies complexity in HBV/HDV infection compared to HBV monoinfection may provide information on the interactions between these two viruses.AIM To compare HBV quasispecies complexity in the HBX 5' region between chronic hepatitis delta(CHD) and chronic HBV mono-infected patients.METHODS Twenty-four untreated patients were included: 7/24(29.2%) with HBeAgnegative chronic HBV infection(CI, previously termed inactive carriers), 8/24(33.3%) with HBeAg-negative chronic hepatitis B(CHB) and 9/24(37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels.The HBX 5' region [nucleotides(nt) 1255-1611] was then PCR-amplified for subsequent next-generation sequencing(MiSeq, Illumina, United States). HBV quasispecies complexity in the region analyzed was evaluated using incidencebased indices(number of haplotypes and number of mutations), abundancebased indices(Hill numbers of order 1 and 2), and functional indices(mutation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them could be the cause of the quasispecies complexity.RESULTS CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL,interquartile range(IQR) 3.5-7.9] than CHD(3.4 logIU/mL, IQR 3-7.6)(P = n.s.)or CI(3.2 logIU/mL, IQR 2.3-3.5)(P < 0.01) patients. The incidence and abundance indices indicated that HBV quasispecies complexity was significantly greater in CI than CHB. A similar trend was observed in CHD patients, although only Hill numbers of order 2 showed statistically significant differences(CHB2.81, IQR 1.11-4.57 vs CHD 8.87, 6.56-11.18, P = 0.038). There were no significant differences in the functional indices, but CI and CHD patients also showed a trend towards greater complexity than CHB. No differences were found for any HBV quasispecies complexity indices between CHD and CI patients. G-to-A and C-to-T nucleotide changes, characteristic of APOBEC3 G, were higher in CHD and CI than in CHB in genotype A haplotypes, but not in genotype D. The proportion of nt G-to-A vs A-to-G changes and C-to-T vs T-to-C changes in genotype A and D haplotypes in CHD patients showed no significant differences. In CHB and CI the results of these comparisons were dependent on HBV genotype.CONCLUSION The lower-replication CHD and CI groups show a trend to higher quasispecies complexity than the higher-replication CHB group. The mechanisms associated with this greater complexity require elucidation. | Cristina Godoy David Tabernero Sara Sopena Josep Gregori Maria Francesca Cortese Carolina González Rosario Casillas Mar?al Yll Ariadna Rando Rosa López-Martínez Josep Quer Gloria González-Aseguinolaza Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías | 2019 | World Journal of Gastroenterology2019，25，13： | 6 |
| 2 | MA-NOS radical sigmoidectomy: report of a transvaginal resection in the human显示文摘 | Antonio M. Lacy Salvadora Delgado Oscar A. Rojas Raúl Almenara Anabel Blasi Josep Llach | 2008 | Surgical Endoscopy2008，，7： | 5 |
| 3 | Analysis of hepatitis B virus preS1 variability and prevalence of the rs2296651 polymorphism in a Spanish population显示文摘AIM To determine the variability/conservation of the domain of hepatitis B virus(HBV) pre S1 region that interacts with sodium-taurocholate cotransporting polypeptide(hereafter, NTCP-interacting domain) and the prevalence of the rs2296651 polymorphism(S267 F, NTCP variant) in a Spanish population. METHODS Serum samples from 246 individuals were included and divided into 3 groups: patients with chronic HBV infection(CHB)(n = 41, 73% Caucasians), patients with resolved HBV infection(n = 100, 100% Caucasians) and an HBV-uninfected control group(n = 105, 100% Caucasians). Variability/conservation of the amino acid(aa) sequences of the NTCPinteracting domain,(aa 2-48 in viral genotype D) and a highly conserved pre S1 domain associated with virion morphogenesis(aa 92-103 in viral genotype D) were analyzed by next-generation sequencing and compared in 18 CHB patients with viremia > 4 log IU/mL. The rs2296651 polymorphism was determined in all individuals in all 3 groups using an in-house real-time PCR melting curve analysis.RESULTS The HBV pre S1 NTCP-interacting domain showed a high degree of conservation among the examined viral genomes especially between aa 9 and 21(in the genotype D consensus sequence). As compared with the virion morphogenesis domain, the NTCPinteracting domain had a smaller proportion of HBV genotype-unrelated changes comprising > 1% of the quasispecies(25.5% vs 31.8%), but a larger proportion of genotype-associated viral polymorphisms(34% vs 27.3%), according to consensus sequences from Gen Bank patterns of HBV genotypes A to H. Variation/conservation in both domains depended on viral genotype, with genotype C being the most highly conserved and genotype E the most variable(limited finding, only 2 genotype E included). Of note, proline residues were highly conserved in both domains, and serine residues showed changes only to threonine or tyrosine in the virion morphogenesis domain. The rs2296651 polymorphism was not detected in any participant.CONCLUSION In our CHB population, the NTCP-interacting domain was highly conserved, particularly the proline residues and essential amino acids related with the NTCP interaction, and the prevalence of rs2296651 was low/null. | Rosario Casillas David Tabernero Josep Gregori Irene Belmonte Maria Francesca Cortese Carolina González Mar Riveiro-Barciela Rosa Maria López Josep Quer Rafael Esteban Maria Buti Francisco Rodríguez-Frías | 2018 | World Journal of Gastroenterology2018，24，6： | 5 |
| 4 | Minimally invasive versus open oesophagectomy for patients with oesophageal cancer: a multicentre, open-label, randomised controlled trial显示文摘 | Surya SAY Biere Mark I van Berge Henegouwen Kirsten W Maas Luigi Bonavina Camiel Rosman Josep Roig Garcia Suzanne S Gisbertz Jean HG Klinkenbijl Markus W Hollmann Elly SM de Lange H Jaap Bonjer Donald L van der Peet Miguel A Cuesta | 2012 | The Lancet . 2012 (9829)2012，，： | 3 |
| 5 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 6 | Long-Term Effects of Sleeve Gastrectomy and Roux-en-Y Gastric Bypass Surgery on Type 2 Diabetes Mellitus in Morbidly Obese Subjects显示文摘 | Amanda Jiménez Roser Casamitjana Lílliam Flores Judith Viaplana Ricard Corcelles Antonio Lacy Josep Vidal | 2012 | Annals of Surgery2012，，6： | 2 |
| 7 | Influence of cirrhosis in cardiac surgery outcomes显示文摘Liver cirrhosis has evolved an important risk factor for cardiac surgery due to the higher morbidity and mortality that these patients may suffer compared with general cardiac surgery population.The presence of contributing factors for a poor outcome,such as coagulopathy,a poor nutritional status,an adaptive immune dysfunction,a degree of cirrhotic cardiomyopathy,and a degree of renal and pulmonary dysfunction,have to be taken into account for surgical evaluation when cardiac surgery is needed,together with the degree of liver disease and its primary complications.The associated pathophysiological characteristics that liver cirrhosis represents have a great influence in the development of complications during cardiac surgery and the postoperative course.Despite the population of cirrhotic patients who are referred for cardiac surgery is small and recommendations come from small series,since liver cirrhotic patients have increased their chance of survival in the last 20 years due to the advances in their medical care,which includes liver transplantation,they have been increasingly considered for cardiac surgery.Indeed,there is an expected rise of cirrhotic patients within the cardiac surgical population due to the increasing rates of non-alcoholic fatty liver disease and non-alcoholic steatohepatitis,especially in western countries.In consequence,a more specific approach is needed in the assessment of care of these patients if we want to improve their management.In this article,we review the pathophysiology and outcome prediction of cirrhotic patients who underwent cardiac surgery. | Juan C Lopez-Delgado Francisco Esteve Casimiro Javierre Josep L Ventura Rafael Maez Elisabet Farrero Herminia Torrado David Rodríguez-Castro Maria L Carrio | 2015 | World Journal of Hepatology2015，7，5： | 2 |
| 8 | l -Carnitine: An adequate supplement for a multi-targeted anti-wasting therapy in cancer显示文摘 | Sílvia Busquets Roberto Serpe Míriam Toledo Angélica Betancourt Enrica Marmonti Marcel Orpí Fabrizio Pin Eva Capdevila Clelia Madeddu Francisco J. López-Soriano Giovanni Mantovani Antonio Macciò Josep M. Argilés | 2012 | Clinical Nutrition2012，，6： | 2 |
| 9 | Software Trace Cache显示文摘 | Alex Ramirez Josep L Larriba-Pey Mato Valero | 2005 | IEEE Transaction on Computers2005，54，1： | 1 |
| 10 | Liver transplantation in patients with portal vein thrombosis显示文摘 | Gerardo Manzanet Fernando Sanjuán Paco Orbis Rafael López ángel Moya Manuel Juan Juanjo Vila Josep Asensi Pedro Sendra José Ru??z Mart??n Prieto José Mir | 2001 | Liver Transplantation2001，，2： | 1 |
| 11 | Software Trace Cache显示文摘 | Alex Ramirez Josep L Larriba-Pey Mato Valero | 2005 | IEEE Transaction on Computers2005，54，1： | 1 |
| 12 | Antimicrobial resistance among gram-negative organisms in the intensive care unirt显示文摘 | Nina C Jan P Josep L | 2003 | Current opinion in critical care2003，9，： | 1 |
| 13 | Seeding rate influence on yield and yield components of irrigated winter wheat in a Mediterranean climate显示文摘 | Jaime L Josep M Javier V | 2004 | Agronomy Journal2004，96，： | 1 |
| 14 | Detection of Maize rough dwarf virus in Spain:a survey of susceptible host genotypes and molecular characterization of two genomic segments of the virus显示文摘 | Vilma O Josep B L Antonio L Q | 2014 | Phytopathologia Mediterranea2014，53，1： | 1 |
| 15 | Plasma Fatty Acid-Binding Protein 4 Increases with Renal Dysfunction in Type 2 Diabetic Patients without Microalbuminuria显示文摘 | Cabré Anna Lázaro Iolanda Girona Josefa Manzanares Josep M Marimón Francesc Plana Núria Heras Mercedes Masana Lluís | 2008 | Clinical Chemistry2008，，1： | 1 |
| 16 | Minimally invasive versus open oesophagectomy for patients with oesophageal cancer: a multicentre, open-label, randomised controlled trial显示文摘 | Surya SAY Biere Mark I van Berge Henegouwen Kirsten W Maas Luigi Bonavina Camiel Rosman Josep Roig Garcia Suzanne S Gisbertz Jean HG Klinkenbijl Markus W Hollmann Elly SM de Lange H Jaap Bonjer Donald L van der Peet Miguel A Cuesta | 2012 | The Lancet2012，，9829： | 1 |
| 17 | Treatment of uterine myoma with5 or 10 mg mifepristone daily during 6 months,post-treatment evolution over 12 months:double-blind randomised clinical trial显示文摘 | Josep L Carbonell E Rita A | 2012 | Eur J Obstet Gynecol Reprod Biol2012，161，2： | 1 |
| 18 | The Barcelona approach: Diagno sis, staging, and treatment of hepatocellular carcinoma显示文摘 | Josep M L Josep F Jordi B | 2004 | Liver Transpl2004，10，21： | 1 |
| 19 | Updated treatment approach to hepatocellular carcinoma显示文摘 | Josep M L | 2005 | J Gastroenterol2005，40，3： | 1 |
| 20 | Effects of valsartan and amlodipine on intrarenal hemodynamics and TGF-β1 plasma levels in a crossover trial in renal transplant recipients显示文摘 | Pablo I Josep M Campistol S L | 2001 | J Am Soc Nephrol2001，12，： | 1 |
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