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| 1 | Aflatoxin B1, zearalenone and deoxynivalenol in feed ingredients and complete feed from different Province in China显示文摘Background: The current study was carried out to provide a reference for monitory of aflatoxin B_1(AFB_1),zearalenone(ZEN) and deoxynivalenol(DON) contamination in feed ingredients and complete feeds were collected from different Province in China from 2013 to 2015.Methods: A total of 443 feed ingredients, including 220 corn, 24 wheat, 24 domestic distillers dried grains with soluble(DDGS), 55 bran, 20 wheat shorts and red dog, 37 imported DDGS, 34 corn germ meal and 29 soybean meal as well as 127 complete feeds including 25 pig complete feed(powder), 90 pig complete feed(pellet), six duck complete feed and six cattle complete feed were randomly collected from different Province in China,respectively, by high-performance chromatography in combined with UV or fluorescence analysis.Results: The incidence rates of AFB_1, ZEN and DON contamination of feed ingredients and complete feeds were80.8, 92.3 and 93.9 %, respectively. The percentage of positive samples for DON ranged from 66.7 to 100 %.Domestic DDGS and imported DDGS presented the most serious contamination AFB_1, ZEN and DON contamination levels of feeds ranged from 61.5 to 100 %, indicated that serious contamination over the studied 3-year period.Conclusion: The current data provide clear evidence that AFB_1, ZEN and DON contamination of feed ingredients and complete feeds in different Province in China is serious and differs over past 3-year. The use of corn, domestic DDGS, imported DDGS and corn germ meal, which may be contaminated with these three mycotoxins, as animal feed may triggered a health risk for animal. Feeds are most contaminated with DON followed by ZEN and AFB_1.Mycotoxins contamination in feed ingredients and complete feeds should be monitored routinely in China. | Li Wu Jianjun Li Yunhu Li Tiejun Li Qinghua He Yulong Tang Hongnan Liu Yongteng Su Yulong Yin Peng Liao | 2017 | Journal of Animal Science and Biotechnology2017，8，2： | 13 |
| 2 | Compensation effects of coated cysteamine on meat quality, amino acid composition, fatty acid composition, mineral content in dorsal muscle and serum biochemical indices in finishing pigs offered reduced trace minerals diet显示文摘Dear Editor.Trace mineral elements(ME)and amino acids have been demonstrated to be essential bioelements in animal nutrition.If there is a deficiency in ME,a number of biological functions in animals may be affected,including physical growth,psychomotor development,and immunity.Supplementation of pig diets with ME is known to improve the animals'growth,reproduction,and their immunity against oxidative stress and cell damage. | Miaomiao Bai Hongnan Liu Kang Xu Xiaofeng Zhang Baichuan Deng Chengquan Tan Jinping Deng Pingping Bing Yulong Yin | 2019 | Science China(Life Sciences)2019，62，11： | 6 |
| 3 | Effects of different levels of urea supplementation on nutrient intake and growth performance in growing camels fed roughage based complete pellet diets显示文摘The utilization of urea in camels has beneficial and negative effects. The aims of this study were to investigate the effects of different levels of urea supplementation on nutrients intake, digestibility,growth performance, feed efficiency and economics in growing camels fed roughage based complete pellet diets. In the present study, eighteen growing camels with an average live body weight of306.17 ± 2.05 kg were randomly assigned in three treatments: T1 = roughage complete pellet diet without urea,T2 = T1 plus 1% urea,and T3 = T1 plus 2% urea. The results showed that the urea supplementation significantly affected average daily feed and nutrient intake of dry matter(DM), organic matter(OM), crude protein(CP), neutral detergent fiber(NDF), and acid detergent fiber(ADF)(P < 0.05).On the contrary, the average daily intake of nitrogen free extract(NFE) and water were not influenced by increasing urea supplementation(P > 0.05). Similarly, digestion coefficient of DM, CP, ether extract(EE),crude fiber(CF) and ADF was influenced by increasing urea level(P < 0.05), while the digestion coefficient of OM, NFE and NDF was not affected by increasing urea level(P > 0.05). The intake of digestive nutrients was similar among all treatment groups. Total body live weight gain and average daily gain were significantly higher in urea supplemented groups(P < 0.05) than in the control group. The supplementation of urea at 1% in low quality roughage complete pellet diets significantly improved(P < 0.05) the feed efficiency. In conclusion,these results indicated that the incorporation of urea at 1% in roughage based complete pellet diets could positively improve nutrients intake, digestibility, growth performance and feed conversion efficiency of growing camels. | Ntiranyibagira Emmanuel Niteen V.Patil Shekbar R.Bhagwat Abdul Lateef Kang Xu Hongnan Liu | 2015 | Animal Nutrition2015，，4： | 6 |
| 4 | Design and experiment of anti-vibrating and anti-wrapping rotary components for subsoiler cum rotary tiller显示文摘The commonly used subsoiling cum rotary tiller machine(SRT)in Northern China is a combination of subsoiler and horizontal rotary tiller,however backfilling of the subsoiling slot,excessive vibration and plant residue wrapping on rotary components has been rarely considered.Therefore,the rotary components and assembly were redesigned to address these issues and to an SRT fitted with IT225 short curve rotary blades behind the V-shape subsoiling slots and IIT245 long curve rotary blades between the tines.Long and short blades were fitted on a rotor in a double helix,with optimal spiral angles of 65° and 90°,and phase angle of 147°and 180°,respectively.Compared with the commonly used SRT(CSRT),the additional anti-wrapping cutting blades in the circumferential and axial direction of ASRT could remove hanging residue on the blade holders,wrapping on the rotor and formation of an isolation layer.Moreover,the cutting edge curve of anti-wrapping cutting blades was an exponential curve.Field tests demonstrated that the redesigned SRT with anti-vibrating and anti-wrapping rotary components(ASRT)had was a significant advancement over the CSRT.Moreover,the working depth of rotary tillage was more stable,while other observations confirmed that backfilling of the subsoiling slot was also improved. | Kan Zheng Allen D.McHugh Hongwen Li Qingjie Wang Caiyun Lu Hongnan Hu Wenzheng Liu Zhiqiang Zhang Peng Liu Jin He | 2019 | International Journal of Agricultural and Biological Engineering2019，12，4： | 4 |
| 5 | A new framework for evaluating along-wind responses of a transmission tower显示文摘In this paper, an analytical framework to evaluate the along-wind-induced dynamic responses of a transmission tower is presented. Two analytical models and a new method are developed: (1) a higher mode generalized force spectrum (GFS) model of the transmission tower is deduced; (2) an analytical model that includes the contributions of the higher modes is further derived as a rational algebraic formula to estimate the structural displacement response; and (3) a new approach, applying load with displacement (ALD) instead of force, to solve the internal force of transmission tower is given. Unlike conventional methods, the ALD method can avoid calculating equivalent static wind loads (ESWLs). Finally, a transmission tower structure is used as a numerical example to verify the feasibility and accuracy of the ALD method. | Liu Guohuan and Li Hongnan School of Civil and Hydraulic Engineering, Dalian University of Technology, Dalian 116023, China PhD Professor | 2009 | Earthquake Engineering and Engineering Vibration2009，8，1： | 3 |
| 6 | Role of hydrogen peroxide in stress-induced programmed cell death during somatic embryogenesis in Fraxinus mandshurica显示文摘We examined how reactive oxygen species, in the form of hydrogen peroxide (H2O2), affect osmotic stress–induced programmed cell death during somatic embryogenesis from cotyledon explants of Manchurian ash (Fraxinus mandshurica Rupr.). We found that substantial osmotic stress was essential for Manchurian ash somatic cells to obtain embryogenic competence. The explant cells displayed hallmarks of programmed cell death, chromatin condensation, and DNA fragmentation to oligonucleotides during somatic embryogenesis. Increasing concentrations of plant growth regulators and sucrose in the medium increased osmotic stress thereby inducing H2O2 accumulation in the explant cells. We found that H2O2 concentration was significantly decreased in explant cells when the induction medium was modified, i.e., when reducing the concentration of sucrose, which reduces the osmotic pressure of the medium, or by withdrawing plant growth regulators at mid-culture. These treatments also decreased the proportion of explant cells undergoing programmed cell death. Accordingly, a decreased rate of somatic embryo induction was observed. These results show that PCD occurred during tissue browning and death of some explant cells during somatic embryogenesis in F. mandshurica. The ROS contributed to PCD in abiotic stress stimulated F. mandshurica cells. | Ling Yang Cheng Wei Chao Huang Hongnan Liu Dongyan Zhang Hailong Shen Yuhua Li | 2019 | Journal of Forestry Research2019，30，3： | 2 |
| 7 | Effects of a nitric oxide donor and nitric oxide scavengers on Sorbus pohuashanensis embryo germination显示文摘The effects of an exogenous nitric oxide donor(sodium nitroprusside, SNP), a NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxode(PTIO) and carboxy-PTIO potassium salt(c PTIO) on the embryo germination of Sorbus pohuashanensis were studied in a petri dish test. SNP at 0.5–5 mmol L-1 increased germination percentage, mean time to germination, germination index and germination energy compared with the control to different degrees. Treatment with 2 mmol L-1 SNP improved germination most significantly; embryo germination percentage for mother tree 1(91.11%) and mother tree 2(64.44%) were much higher than the control. In addition,excessive SNP levels did not enhance embryo germination.Combined treatment with SNP and an NO scavenger delayed embryo germination. Treatment with c PTIO inhibited embryo germination; germination percentage was 42.22% and was lower than that of the control. These results show that low concentrations of exogenous NO can enhance the embryo germination of S. pohuashanensis,providing a simple, effective way for promoting germination of S. pohuashanensis. | Ling Yang Dongyan Zhang Hongnan Liu Cheng Wei Jianan Wang Hailong Shen | 2018 | Journal of Forestry Research2018，29，3： | 2 |
| 8 | Reduced dietary nitrogen with a high Lys:CP ratio restricted dietary N excretion without negatively affecting weaned piglets显示文摘We hypothesized that balancing the content of exogenous amino acids, especially lysine, to reduce protein content in swine diets could reduce nitrogen(N) pollution associated with animal husbandry. Two experiments(45 d each experiment) were performed on weaned piglets(Duroc x Landrace x Yorkshire,28 d of age) to test this and to determine the optimal lysine to crude protein(Lys:CP) ratio in diet. In Exp. 1,12 piglets(6 replicates [n = 6]) were fed diets containing different levels of CP(17% and 20%) but the same level of Lys, Increased CP content resulted in significant increases(P < 0.05) of average daily gain(ADG), average daily feed intake(ADFI), and body weight(BW);but did not affect the feed to gain ratio. In Exp. 2,24 piglets(8 replicates [n = 8]) were fed 1 of 3 diets as follows: 1) 20% CP with a regular Lys:CP ratio(6.23%, control);2) 17% CP with a reduced Lys:CP ratio(6.14%, LL);or 3) 17% CP with a standard Lys:CP ratio(7.32%, SL). The ADG, final BW, serum concentrations of growth hormone and insulin-like growth factor-1, villus height in the jejunum, and villus height to crypt depth ratio were the lowest in piglets fed LL diet, whereas blood urea N concentration was the lowest and the value of lipase activity was the highest in the piglets fed SL diet. The SL diet did not affect growth performance,intestinal morphology, or serum hormone concentrations, indicating that reduced dietary N with a high Lys:CP ratio can efficiently reduce dietary N excretion without negatively affecting weaned piglets. | Hongnan Liu Li Wu Hui Han Yuying Li Lijian Wang Jie Yin Wenjun Fan Miaomiao Bai Jiming Yao Xingguo Huang Tiejun Li | 2019 | Animal Nutrition2019，，2： | 2 |
| 9 | Relationship between H_(2)O_(2) accumulation and NO synthesis during osmotic stress:promoted somatic embryogenesis of Fraxinus mandshurica显示文摘Osmotic stress promotes somatic embryogenesis of Fraxinus mandshurica,which leads to accumulation of reactive oxygen species(ROS).The single pieces of cotyledons of F.mandshurica were used as explants to induce somatic embryogenesis in osmotic-stress medium.Furthermore,the hydrogen peroxide H_(2)O_(2) content of explanted cells was varied by adding exogenous H_(2)O_(2) or catalase solution to assess the effects of the exogenous H_(2)O_(2)on somatic embryogenesis,intracellular H_(2)O_(2)accumulation,and the relationship between signaling mediated by ROS or reactive nitrogen species.The results revealed that exogenous H_(2)O_(2)(100?300μmol L^(–1))increased the number of somatic embryos.On 60th day of exogenous H_(2)O_(2)(200μmol L^(–1))treatment,the number of somatic embryos of explants treated,which was 136.54%,was higher than the control.Moreover,exogenous H_(2)O_(2)(100μmol L^(–1))significantly increased the intracellular H_(2)O_(2)content and enhanced the activities of superoxidase dismutase and peroxidase.Finally,exogenous H_(2)O_(2)(100μmol L^(–1))activated the intracellular non-enzymatic pathway for nitric oxide(NO)synthesis.The somatic embryogenesis in broadleaf trees increases with the change of endogenic ROS content,and depends on the upregulation of antioxidant enzymes.Both H_(2)O_(2)and NO,as signaling molecules,were found to be involved in the process of somatic embryogenesis in broadleaf trees.In the process of exogenous H_(2)O_(2)promoting somatic embryogenesis,NO synthesis depended on non-enzymatic reactions.These results provide a scientific basis for resolving the mechanism by which ROS levels are regulated during somatic embryogenesis of broadleaf trees and establish a reasonable and efficient technology system for regulating somatic embryogenesis of trees. | Ling Yang Hanyang Guo Yingying Liu Dongyan Zhang Hongnan Liu Hailong Shen | 2021 | Journal of Forestry Research2021，32，3： | 1 |
| 10 | Strain rate effect on transmission tower-line system under earthquake action显示文摘 | Wenming Wang Hongnan Li Guohuan Liu Tian Li | 2011 | Advanced Materials Research2011，，： | 1 |
| 11 | Optimisation of aqueous two-phase extraction of anthocyanins from purple sweet potatoes by response surface methodology显示文摘 | Liu Xingli Mu Taihua Sun Hongnan | 2013 | Food chemistry2013，141，3： | 1 |
| 12 | Optimisation of aqueous two-phase extraction of anthocyanins from purple sweet potatoes by response surface methodology 显示文摘 | Xingli Liu Taihua Mu Hongnan Sun | 2013 | Food Chemistry2013，141，3： | 1 |
| 13 | Optimisation of aqueous two-phase extraction of anthocyanins from purple sweet potatoes by response surface methodology显示文摘 | LIU Xingli MU Taihua SUN Hongnan | 2013 | Food Chemistry2013，141，3： | 1 |
| 14 | Predicting the Useof Pirated Software:A Contingency Model IntegratingPerceived Risk with the Theory of Planned Behavior显示文摘 | Liao Chechen Lin Hongnan Liu Yuping | 2010 | Journal of Business Ethics2010，91，2： | 1 |
| 15 | The molecular tumor burden index as a response evaluation criterion in breast cancer显示文摘Circulating tumor DNA(ctDNA)is a potential biomarker of prognosis and therapeutic response.We conducted this study to explore the role of the molecular tumor burden index(mTBI)in ctDNA as a therapeutic response and prognostic biomarker in a larger cohort prospective phase III randomized multicenter study.We collected 291 plasma samples from 125 metastatic breast cancer patients from the CAMELLIA study(NCT01917279).Target-capture deep sequencing of 1021 genes was performed to detect somatic variants in ctDNA from the plasma samples.The pretreatment mTBI value was correlated with tumor burden(P=0.025).Patients with high-level pretreatment mTBI had shorter overall survival than patients with low-level pretreatment mTBI,and the median overall survival was 40.9 months and 68.4 months,respectively(P=0.011).Patients with mTBI decrease to less than 0.02%at the first tumor evaluation had longer progression-free survival and overall survival(P<0.001 and P=0.007,respectively).The mTBI has good sensitivity to identify complete response/partial response and progressive disease based on computed tomography scans(88.5%and 87.5%,respectively).The patients classified as molecular responders had longer progression-free survival and overall survival than the nonmolecular responders in the overall cohort(P<0.001 and P=0.036,respectively),as well as in the cohort in which computed tomography scans were defined as representing stable disease(P=0.027 and P=0.015,respectively).The mTBI in ctDNA detected in liquid biopsies is a potential biomarker of therapeutic response and prognosis in patients with metastatic breast cancer. | Zongbi Yi Fei Ma Guohua Rong Binliang Liu Yanfang Guan Jin Li Xiaoying Sun Wenna Wang Xiuwen Guan Hongnan Mo Jiani Wang Haili Qian Binghe Xu | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 1 |
| 16 | Inelastic structural control based on MBC and FAM显示文摘 | Li Gang Liu Qifeng Li Hongnan | 2011 | Mathematical Problems in Engineering2011，，1： | 1 |
| 17 | Strain rate effect on transmission tower-line system under earthquake action 显示文摘 | Wang Wenming Li Hongnan Liu Guohuan | 2011 | Ad- vanced Materials Research2011，，： | 1 |
| 18 | Anti-blocking performance of ultrahigh-pressure waterjet assisted furrow opener for no-till seeder显示文摘No-till planting method is widely used for maize-wheat two-crops-a-year area in the North China Plain.However,cruel soil conditions,especially the large number of maize stalks which are hard to cutoff covering,often cause an unsatisfying planting quality.Based on the authors’previous investigation,ultrahigh-pressure(UHP)waterjet is capable to solve this problem and obtain qualified seedbeds.Thus,a UHP waterjet assisted furrow opener for no-till seeder was designed.Field tests showed that double-disc furrow openers worked well with UHP waterjet,since the sharpened disc blades could help to cut soil and residue,meanwhile,minimize soil disturbance.Response surface method(RSM)was used to investigate the relationship among forward speed,waterjet pressure,jet impingement angle and anti-blocking performance(stalks cutoff ratio and depth of soil cutting),and a Box-Behnken three-factor design was used to identify the optional operation parameters.A total of 17 combinations were conducted,and the results showed all three operation parameters significantly affected anti-blocking performance.Stalks cutoff ratio and depth of soil cutting increased with the increase of waterjet pressure,jet impingement angle,and decreased with the increase of forward speed.The optimization analysis indicated that when waterjet pressure was 267-280 MPa,jet impingement angle was 80.2°to 90.0°and forward speed was 4.00-4.42 km/h,the overall performance of UHP waterjet assisted double-disc furrow opener for no-till seeder was maximized.Stalks cutoff ratio could be above 95%and no blockage occurred.This study may provide a new approach and reference for the anti-blocking technology of no-tillage seeding. | Hongnan Hu Hongwen Li Qingjie Wang Jin He Caiyun Lu Yingbo Wang Peng Liu | 2020 | International Journal of Agricultural and Biological Engineering2020，13，2： | 1 |
| 19 | A water-soluble β-glucan improves growth performance by altering gut microbiome and health in weaned pigs显示文摘Beta-glucan has been shown to have a beneficial effect on gastrointestinal health.This experiment was conducted to investigate the effects ofβ-glucan isolated from Agrobacterium sp.ZX09 on growth performance and intestinal health of weaning pigs.A total of 108 weaned pigs(21 d of age;6.05±0.36 kg)were randomly divided into 3 groups(6 pens/group;6 pigs/pen),and the groups were each treated with the following diets:1)basal diet,2)basal diet supplemented with 20 mg/kg olaquindox,3)basal diet supplemented with 200 mg/kgβ-glucan,for 21 d.Compared with the control group,pigs fed with200 mg/kgβ-glucan had greaterBW,average daily gain and duodenal villus height to crypt depth ratio(P<0.05).Olaquindox increased the duodenal or jejunal villus height of pigs compared withβ-glucan.Compared with the control group,β-glucan tended to increase the occludin mRNA expression in the jejunum(0.05 | Yuliang Wu Xue Li Hongnan Liu Yanjun Du Jian Zhou Lijun Zou Xia Xiong Huilin Huang Zhiliang Tan Yulong Yin | 2021 | Animal Nutrition2021，，4： | 0 |
| 20 | Research progress of rubrene as an excellent multifunctional organic semiconductor显示文摘Rubrene,a superstar in organic semiconductors,has acliieved imprecedented achievements in the application of electronic devices,and research based on its various photoelectric properties is still in progress.In this review,we introduced the preparation of rubrene crystal,summarized the applications in organic optoelectronic devices with the latest research achievements based on rubrene semiconductors.An outlook of future research directions and cliallenges of rubrene semiconductor for applications is also provided. | Si Liu Hongnan Wu Xiaotao Zhang Wenping Hu | 2021 | Frontiers of physics2021，16，1： | 0 |
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