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| 1 | Depletion of CD25^+CD4^+T cells (Tregs) enhances the HBV-specific CD8^+ T cell response primed by DNA immunization显示文摘AIM: Persistent hepatitis B virus (HBV) infection is characterized by a weak CD8+ T cell response to HBV. Immunotherapeutic strategies that overcome tolerance and boost these suboptimal responses may facilitate viral clearance in chronically infected individuals. Therefore, we examined whether CD25+CD4+ regulatory T (Treg) cells might be involved in a inhibition of CD8+T cell priming or in the modulation of the magnitude of the'peak' antiviral CD8+ T cell response primed by DNA immunization. METHODS: B10.D2 mice were immunized once with plasmid pCMV-S. Mice received 500 μg of anti-CD25 mAb injected intraperitoneally 3 d before DNA immunization to deplete CD25+ cells. Induction of HBV-specific CD8+ T ceils in peripheral blood mononuclear cells (PBMCs) was measured by S28-39 peptide loaded DimerX staining and their function was analyzed by intracellular IFN-γ staining.RESULTS: DNA immunization induced HBV-specific CD8+ T cells. At the peak T cell response (d 10), 7.1±2.0% of CD8+ T cells were HBV-specific after DNA immunization, whereas 12.7±3.2% of CD8+ T cells were HBV-specific in Treg-depleted mice, suggesting that DNA immunization induced more antigen-specific CD8+ T cells in the absence of CD25+ Treg cells (n = 6, P<0.05). Similarly, fewer HBVspecific memory T cells were detected in the presence of these cells (1.3±0.4%) in comparison to Treg-depleted mice (2.6±0.9%) on d 30 after DNA immunization (n = 6, P<0.01). Both IFN-γ production and the avidity of the HBV-specific CD8+ T cell response to antigen were higher in HBV-specific CD8+ T cells induced in the absence of Treg cells.CONCLUSION: CD25+ Treg cells suppress priming and/or expansion of antigen-specific CD8+ T cells during DNA immunization and the peak CD8+ T cell response is enhanced by depleting this cell population. Furthermore, Treg cells appear to be involved in the contraction phase of the CD8+ T ceil response and may affect the quality of memory T cell pools. The elimination of Treg cells or their inhibition may be important in immunotherapeutic strategies to control HBV infection by inducing virus-specific cytotoxic T lymphocyte responses in chronically infected subjects. | Yoshihiro Furuichi Hirotake Tokuyama Satoshi Ueha Makoto Kurachi Fuminori Moriyasu Kazuhiro Kakimi | 2005 | World Journal of Gastroenterology2005，11，24： | 30 |
| 2 | Arterial enlargement, tortuosity, and intimal thickening in response to sequential exposure to high and low wall shear stress显示文摘 | Eiketsu Sho Hiroshi Nanjo Mien Sho Mikio Kobayashi Masayo Komatsu Koichi Kawamura Chengpei Xu Christopher K Zarins Hirotake Masuda | 2004 | Journal of Vascular Surgery2004，，3： | 2 |
| 3 | Development of High - Frequency SiC - MESFETs 显示文摘 | Manabu Arai Hirotake Honda Shuichi Ono | 2003 | Electronics and Communications in Japan2003，86，11： | 1 |
| 4 | A low-grade increase of serum pancreatic exocrine enzyme levels by dipeptidyl peptidase-4 inhibitor in patients with type 2 diabetes显示文摘 | Hirotake Tokuyama Harukiyo Kawamura Masaki Fujimoto Kazuki Kobayashi Mie Nieda Tetsuya Okazawa Minoru Takemoto Fumio Shimada | 2013 | Diabetes Research and Clinical Practice2013，，3： | 1 |
| 5 | Hot isostatie pressing of (2 mol% Y2O3) composite powders显示文摘 | Muraoka Y Yoshinaka M Hirotak K | 1996 | Mater Res Bull1996，31，： | 1 |
| 6 | Inhibitory Effects of Cnitro and Cnitroso Compounds Formed by Reaction of Sorbic Acid with Sodium Nicrite显示文摘 | Achiwa Yumiko Hibasami Hiroshige Katsuzaki Hirotaks | 1996 | Nippon Shokuhin Kagaku Kogaku Kaishi1996，43，5： | 1 |
| 7 | Reduced expression of the Syk gene is correlated with poor prognosis in human breast cancer显示文摘 | Tatsuya Toyama Hirotake Lwase Hiroko Yamashita | 2003 | Science2003，189，： | 1 |
| 8 | Muhiresidue analysis of pesticides and fruits using two-layered column with graphitized carbon and water absorbent polymer显示文摘 | Hirotake Obana | 2001 | Analyst2001，126，： | 1 |
| 9 | Preparation and characterization of temperature-responsive magnetite nanoparticles conjugated with N -isopropylacrylamide-based functional copolymer显示文摘 | Hirotake Wakamatsu Kazuya Yamamoto Aiko Nakao Takao Aoyagi | 2005 | Journal of Magnetism and Magnetic Materials2005，，2： | 1 |
| 10 | Development of high-frequency SiC-MESFETs显示文摘 | MANABU A HIROTAKE H SHUICHI O | 2003 | IEICE Trans on Electronica Pt22003，86，4： | 1 |
| 11 | DJ-1, a Novel Oncogene Which Transforms Mouse NIH3T3 Cells in Cooperation with ras显示文摘 | Daisuke Nagakubo Takahiro Taira Hirotake Kitaura Masako Ikeda Katsuyuki Tamai Sanae M.M. Iguchi-Ariga Hiroyoshi Ariga | 1997 | Biochemical and Biophysical Research Communications1997，，2： | 1 |
| 12 | Vapor pressures of diethyl,diisopropyl, and dibutyl phthalates at reduced pressures显示文摘 | HIROTAKE KATAYAMA | 1988 | Bull Chem Soc Jpn1988，61，9： | 1 |
| 13 | Approaches of output improvement for a cesium vapor laser pumped by a volume - Bragg- grating coupled laser - diode - array 显示文摘 | You Wang Minoru Niigaki Hirotake Fukuoka | 2007 | Physics Letters A2007，，360： | 1 |
| 14 | Integration of organic electroluminescent diodes and polymeric waveguide devices: characterization of light source for optical integrated circuit显示文摘 | Yutaka Ohmori Hirotake Kajii Takahisa Tsukagawa | 2001 | SPIE2001，4439，： | 1 |
| 15 | Carbon materials syntheses using dielectric barrier discharge microplasma in supercritical carbon dioxide environments 显示文摘 | TAKAAKI T KEN K HIROTAKE K | 2007 | The Journal of Supercritical Fluids2007，41，3： | 1 |
| 16 | DJ1, a novel oncogene which transforms mouse N1H3'I3 cells in cooperation with ras显示文摘 | Daisuke Nagakubo Takahiro Taira Hirotake Kitaura | 1997 | Biochem Biophys Res Comm1997，231，： | 1 |
| 17 | Multi probe method for straightness profile measurement based on least uncertainty propagation ( 2nd report )-Two - point method considering cross - axis translational motion, pitch motion and sensor's random error显示文摘 | Eiki Okuyama Hirotake Akata Hiromi Ishikawa | 2010 | Precision Engineer- ing2010，34，： | 1 |
| 18 | Complete obstruction of the lower common bile duct caused by autoimmune pancreatitis: is biliary reconstruction really necessary?显示文摘 | Toshiki Matsubara Yoichi Sakurai Hirotake Miura Hidetaka Kobayashi Mitsutaka Shoji Yasuko Nakamura Hiroki Imazu Shigeru Hasegawa Masahiro Ochiai Takahiko Funabiki | 2005 | Journal of Hepato - Biliary - Pancreatic Surgery2005，，1： | 1 |
| 19 | Histopathological analyses of the antitumor activity of anti-glypican-3 antibody (GC33) in human liver cancer xenograft models: The essential role of macrophages显示文摘 | Hirotake Takai Atsuhiko Kato Yasuko Kinoshita Takahiro Ishiguro Yayoi Takai Yoshimi Ohtani Masamichi Sugimoto Masami Suzuki | 2009 | Cancer Biology & Therapy2009，，： | 1 |
| 20 | Do Local anaesthetics interact with dihydropyridine binding sites on neuronal L-type Ca~(2+) Channels? 显示文摘 | Hirotak Browne T Appadu BL | 1997 | Br J Anaesth1997，78，： | 1 |
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