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| 1 | Regulatory effect and mechanism of gastrin and its antagonists on colorectal carcinoma显示文摘AIM To explore the effect and mechanism ofgastrin and its antagonists proglumide and somatostatin on coIorectal carcinoma and their clinical significance.METHODS A model of transplanted human colonic carcinoma was established from SW480cell line in gymnomouse body. The volume andweight of transplanted carcinoma was observedunder the effect of pentagatrin (PG), proglumide (PGL) and octapeptide somotostatin (SMS201-995, SMS). The cAMP content ot carcinoma cell was determined by redioimmunoassay and the DNA, protein content and cell cycle were determined by flow-cytometry. The amount of viabIe cells was determined by MTT colorimetric analysis, lP3 content was determined by radioimmunoassay, Ca2+ concentration in cell by fluorometry and PKC activity by isotopic enzymolysis. The expression of gastrin, c-myc,C-fos and rasP21 in 48 cases of colorectal carcinoma tissue was detected by the immunocytochemistry SP method. Argyrophilianucleolar organizer regions was determined withargyrophilia stain.RESULTS The volume, weight, cAMP, DNAand protein content in carcinoma cell, cellamount and proliferation index of S and G2Mphase in PG group were all significantly higher than those of control group. When PG was at theconcentration of 25 mg/ L, the amount of viablecells, lP3 content and Ca2+ concentration in celland membrane PKC activity in PG group weresigniticantly higher than those in control group;when PGL was at a concentration ot 32 mg/L,they dropped to the lowest level in PG (25 mg/L)+ PGL group, but without significant differencefrom the control group. The positive expression rate of gastrin, c-myc, c-fos and rasp21 in carcinoma tissue was 39 .6%, 54 .2%, 47. 9% and54 .2% resPectively and significantly higher than that in mucosa 3 cm and 6 cm adjacent tocarcinoma tissue and normal colorectal mucosa.The positive expression rate of gastrin of highlydifferentiated adenocarcinoma group was significantly higher than that of poorly differentiated and mucinous adenocarcinoma groups. The AgNORs count of carcinoma tissue was significantly higher than that in mucosa 3 cm and 6 cm adjacent to carcinoma tissue and normal colorectal mucosa; and the positive expression of c-myc and c-fos and the AgNORs count in gastrin-positive group was significantly higher than those in gastrin-negative group.CONCLUSION Pentagastrin has a promoting effect on the growth of transplanted human colonic carcinoma from SW480 cell line. PGL hasno obvious effect on the growth of human colonic carcinoma SW480 cell line, but couldinhibit the growth-promoting effect of PG on transplanted carcinoma. Somatostatin can not only inhibit the growth of transplanted human colonic carcinoma from SW480 cell line directlybut also depress the growth-promoting effect ofgastrin on the transplanted carcinoma. Somecolorectal carcinoma cells can produce and secrete gastrin through autocrine, highly-differentiated adenocarcinoma express the highest level gastrin. Endogenous gastrin can stimulate the cell division and proliferation of carcinoma cell and promote the growth of colorectal carcinoma regulating the expression of oncogene omyc, c-fos. Our study has provided experimental basis for the adjuvant treatment using gastrin antagonist such as PGL,somatostatin of patients with colorectalcarcinoma. | He SW Shen KQ He YJ Xie B Zhao YM | 1999 | World Journal of Gastroenterology1999，5，5： | 20 |
| 2 | Backward Propagation of Otoacoustic Emissions显示文摘Normal mammalian ears not only detect but also generate sounds. The ear-generated sounds, i.e., otoacoustic emissions (OAEs), can be measured in the external ear canal using a tiny sensitive microphone. In spite of wide applications of OAEs in diagnosis of hearing disorders and in studies of cochlear functions, the question of how the cochlea emits sounds remains unclear. The current dominating theory is that the OAE reaches the cochlear base through a backward traveling wave. However, recently published works, including experimental data on the spatial pattern of basilar membrane vibrations at the emission frequency, demonstrated only forward traveling waves and no signs of backward traveling waves. These new findings indicate that the cochlea emits sounds through cochlear fluids as compression waves rather than through the basilar membrane as backward traveling waves. This article reviews different mechanisms of the backward propagation of OAEs and summarizes recent experimental results. | HE Wenxuan,1, 2 REN Tianying,1, 21. Oregon Hearing Research Center, Department of Otolaryngology and Head & Neck Surgery, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, NRC04, Portland, Oregon 97239 USA 2. School of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi 710061 China | 2006 | Journal of Otology2006，1，1： | 4 |
| 3 | microRNAs join the p53 network--another piece in the tumour-suppression puzzle显示文摘 | He L He X Lowe SW | 2007 | Nat Rev Cancer2007，7，11： | 1 |
| 4 | A microRNA polycistron as a potential human oncogene显示文摘 | He L Thomson JM Hemann MT Hernando-Monge E Mu D Goodson S Powers S Cordon-Cardo C.Lowe SW Hannon GJ | | 0，，： | 1 |
| 5 | MicroRNA-1 negatively regulates expression of the hypertrophy-associated calmodulin and Mef2a genes显示文摘 | Ikeda S He A Kong SW | 2009 | Mol Cell Biol2009，29，8： | 1 |
| 6 | Stabilization of the P53 tumor suppressor is induced by adenovirus EIA and accompanies apoptosis 显示文摘 | | 1993 | Genes Dev1993，7，4： | 1 |
| 7 | microRNAs join the P53 network- another piece in the tumour-suppression puzzle显示文摘 | He L He X Lowe SW | 2007 | Nat Rev Cancer2007，7，11： | 1 |
| 8 | Porous titanium(Ti) scaffolds by freezing TiH2/camphene slurries显示文摘 | YOOK SW YOON BH KIM HE KON YH KIM YS | 2008 | Materials Letters2008，62，30： | 1 |
| 9 | Co-occupancy by multiple cardiac transcription factors identifies transcriptional enhancers active in heart显示文摘 | He A Kong SW Ma Q | 2011 | Proc Natl Acad Sci U S A2011，108，14： | 1 |
| 10 | microRNAs join the p53 network-another piece in the tumour-suppression puzzle 显示文摘 | He L He X Lowe SW | 2007 | Nat Rev Cancer2007，7，： | 1 |
| 11 | Prognostic implications of type and density of tumour-infiltrating lymphocytes in gastric cancer显示文摘 | Lee HE Ghae SW Lee YJ | 2008 | Br J Gancer2008，99，10： | 1 |
| 12 | MicroRNA-1 negatively regulates expression of the hypertrophy-associated calmodulin and MEF2a genes 显示文摘 | Ikeda S He A Kong SW | 2009 | Mol Cell Biol2009，29，8： | 1 |
| 13 | P53-dependent apoptosis modulates the cytotoxicity of anticancer drugs显示文摘 | Lowe SW Ruley HE Jacks T | 1993 | Cell1993，74，7： | 1 |
| 14 | In situ gelling stimuli-sensitive block copolymer hydrogels for drug delivery 显示文摘 | He C Kim SW Lee DS | 2008 | J Control Release2008，127，3： | 1 |
| 15 | Methotrexate-induced mala bsorption in children with acute lymphoblastic leukemia显示文摘 | Craft SW Kay HE Lawsonl DN | 1977 | Br Med J1977，，： | 1 |
| 16 | microRNAs join the p53 network - ano- ther piece in the tumour - suppression puzzle 显示文摘 | He L He X Lowe SW | 2007 | Nat Rev Cancer2007，7，11： | 1 |
| 17 | Long-term results of a multicenter study on sacral nerve sti- mulation for treatment of urinary urge incontinence, urgency-frequency,and retention 显示文摘 | Siegel SW Gatanzaro F Dijkema HE | 2000 | Urology2000，56，6： | 1 |
| 18 | The impact of anemia on the prognosisof chronic heart failure: a meta analysis and systemicreview显示文摘 | He SW Wang LX | 2009 | Congest Heart Fail2009，15，3： | 1 |
| 19 | p53-dependent apoptosis modulates the cytotoxicity of anticancer agents显示文摘 | Lowe SW Ruley HE Jacks T | 1993 | Cell1993，74，6： | 1 |
| 20 | P53-dependent apoptosis modulates the cytotoxity of anticancer anticancer agents显示文摘 | Ruley He Jacks T | 1993 | Cell1993，74，6： | 1 |
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