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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | Cloning and identification of an angiostatic molecule IP-10/crg-2显示文摘AIM To obtain human and murine cDNAsencoding IFN--y inducible protein 10 (lP--10) andcytokine responsive gene--2 (Crg-2).METHODS The encoding genes of lP-10 and Crg2 were amplified by RT--PCR from Cultured humanfibroblast cells and Balb/ c mouse liver treatedby IFN-y and TNF-a, respectively, and clonedinto plasmids of PUC19 and PGEM3Zf(+ ).RESULTS The nucleotide sequences ot theamplified DNA were confirmed by endonucleasesdigestion and sequencing.CONCLUSION Recombinant lP-10/ crg--2 geneclones with 306 hp and 314 hp inserts wereestablished for further research on biologicalactivities and ligands of hiP-10/mCrg--2. | Liu ZG Yang JH An HZ Wang HY He FT Han ZY Han Y Wu HP Xiao B Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 2 |
| 3 | Impact of sitagliptin on markers of beta-cell function:a meta-analysis显示文摘 | Riche DM East HE Riche KD | | 0，，05： | 1 |
| 4 | In-vitro antitumor activity evaluation of hyperforin derivatives显示文摘 | Sun F Liu JY He F Liu Z Wang R Wang DM | 2011 | J Asian Nat Prod Res2011，13，8： | 1 |
| 5 | Etiology of temporomandibular joint ankylosis secondary to condylar fractures: the role of con- comitant mandibular fractures 显示文摘 | He DM Ellis Ill E Zhang Y | 2008 | J Oral Maxillofac Surg2008，66，： | 1 |
| 6 | Finite element analysis of antiimpact loading of wrist protectors显示文摘 | Dong XP Wang DM He JY | 2011 | J Clin Rehab Tiss Eng Res2011，15，30： | 1 |
| 7 | Molecular genetic assay ofmucopolysaccharidosis IVA in south China 显示文摘 | HE DM HUANG YL OU ZY | 2013 | Gene2013，532，1： | 1 |
| 8 | Targeting glioblastoma stem cells: cell surface markers 显示文摘 | He J Liu Y Lubman DM | 2012 | Curr Med Chem2012，19，35： | 1 |
| 9 | Differential regulation of angiotensin II-induced expression of connective tissue growth factor by protein kinase C isoforms in the myocardium显示文摘 | He Z Way KJ Arikawa E Chou E Opland DM Clermont A | 2005 | J Biol Chem2005，280，15： | 1 |
| 10 | Clinicopathologic study after submacular removal of choroidal neovascular membranes treated with verteporfin ocular photodynamic therapy显示文摘 | Moshfeghi DM Kaiser PK Grossnlklaus HE | 2003 | Am J Ophthalmol2003，135，3： | 1 |
| 11 | The Society of Thoracic Surgeons isolated aortic valve replacement (AVR) composite score: a report of the STS quality measurement task force 显示文摘 | Shahian DM He X Jacobs JP | 2012 | Ann Thorac Surg2012，94，6： | 1 |
| 12 | Clinical investigation of early post - traumatic temporomandibular joint ankylosis and the role of repositioning discs in treatment 显示文摘 | Zhang Y He DM | 2006 | Int J Oral Maxillofac Surg2006，35，12： | 1 |
| 13 | The long-term effect of splenorenal shunt combined with disconnection on portal hypertension显示文摘 | Gao DM He IS Wang JS | 1998 | Chin J Surg1998，36，3： | 1 |
| 14 | Conditions affecting hydrodynamics - based gene delivery into mouse liver in vivo 显示文摘 | Feng DM He CX Miao CY | 2004 | Clin Exp Pharmacol Physiol2004，31，12： | 1 |
| 15 | Isolation and characterization of an extracellular heat-stable lipase produced by Pseudomonas fluorescens MC50显示文摘 | Bozoglu F Swaisgood HE Adams DM Jr | 1984 | Agricultural and Food Chemistry1984，32，1： | 1 |
| 16 | Enhanced CD4+ T-cell recovery with earlier HIV-1 antiretroviral therapy 显示文摘 | Le T Wright Smith DM He W Catano G Okulicz JF Young JA Clark RA Richman DD Little SJ Ahuja SK | 2013 | N Engl J Med2013，368，3： | 1 |
| 17 | The role of cancer stem cells in neoplasia of the lung:past,present and fu- ture显示文摘 | Yagui-BehranA He B Jablons DM | 2008 | Clinical & Translational Oncology2008，10，11： | 1 |
| 18 | Clinical investigation of early post-traumatic temporomandibular joint ankylosis and the role of repositioning discs in treaunent显示文摘 | Zhang Y He DM | 2006 | Int J Oral Maxillofac Surg2006，35，12： | 1 |
| 19 | Self-reported menopausal symptoms, coronary artery calcification, and carotid inti- ma-media thickness in recently menopausal women screened for the Kronos early estrogen prevention study (KEEPS) 显示文摘 | Wolff EF He Y Black DM | 2013 | Fertility and Sterility2013，99，5： | 1 |
| 20 | Clinicopathologic study after submacular removal of choroidal neovascularmembranes treated with verteporfin ocular photodynamie therapy显示文摘 | Moshfeghi DM Kaiser PK Gressniklaus HE | 2003 | AmJ Ophthalmol2003，135，3： | 1 |
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