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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | Cloning and identification of an angiostatic molecule IP-10/crg-2显示文摘AIM To obtain human and murine cDNAsencoding IFN--y inducible protein 10 (lP--10) andcytokine responsive gene--2 (Crg-2).METHODS The encoding genes of lP-10 and Crg2 were amplified by RT--PCR from Cultured humanfibroblast cells and Balb/ c mouse liver treatedby IFN-y and TNF-a, respectively, and clonedinto plasmids of PUC19 and PGEM3Zf(+ ).RESULTS The nucleotide sequences ot theamplified DNA were confirmed by endonucleasesdigestion and sequencing.CONCLUSION Recombinant lP-10/ crg--2 geneclones with 306 hp and 314 hp inserts wereestablished for further research on biologicalactivities and ligands of hiP-10/mCrg--2. | Liu ZG Yang JH An HZ Wang HY He FT Han ZY Han Y Wu HP Xiao B Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 2 |
| 3 | Quercetin inhibits UV irradiation-induced inflammatory cytokine production in primary human keratinocytes by suppressing NF-κB pathway显示文摘 | Vicentini FT He T Shao Y | | 0，，03： | 1 |
| 4 | Effect of crocin on experimental atherosclerosis in quails and its mechanisms 显示文摘 | He SY Qian ZY Tang FT | 2005 | Life Sci2005，77，： | 1 |
| 5 | Effect of crocin on ex- perimental atherosclerosis in quails and its mechanisms 显示文摘 | He SY Qian ZY Tang FT | 2005 | Life Sci2005，77，8： | 1 |
| 6 | Effect of hypobaric conditions on ethylene evolution and growth of lettuce and wheat 显示文摘 | He C J Davies Jr FT Lacey RE | 2003 | Journal of Plant Physiology2003，160，11： | 1 |
| 7 | Clathrin-media-ted endocytosis of the beta-adrenergic receptor is regulated by phosphorylation/dephosphorylation of beta-arrestinl显示文摘 | Lin FT Krueger KM Kendall HE | | 0，，： | 1 |
| 8 | Separating the effects of hy- pobaria and hypoxia on lettuce: growth and gas exchange 显示文摘 | He C J Davies Jr FT Lacey RE | 2007 | Physiologia Plantarum2007，131，： | 1 |
| 9 | Effect of hypobaric conditions on ethylene evolution and growth of lettuce and wheat显示文摘 | He C Davies FT Lacey RE | 2003 | Journal of Plant Physiology2003，160，11： | 1 |
| 10 | Effect of crocin on experimental atherosclerosis in quails and its mechanisms显示文摘 | He SY Qian ZY Tang FT | 2005 | Life Sci2005，77，8： | 1 |
| 11 | Effect of crocin on experimental atherosclerosis in quails and its mechanisms显示文摘 | He SY Qian ZY Tang FT | 2005 | Life Sci2005，77，： | 1 |
| 12 | Downregulation of endothelin-1 by farnesoid X receptor in vascular endothelial cells显示文摘 | He FT Li J Mu Y | 2006 | Circ Res2006，98，： | 1 |
| 13 | Neurogenesis decreases during brain maturation from adolescence to adulthood 显示文摘 | He J Crews FT | 2007 | Pharmacol Biochem Behav2007，86，2： | 1 |
| 14 | Neurogenesis decreases during brain maturation from adolescence to adulthood 显示文摘 | He J Crews FT | 2007 | Pharmacol Biochem Behav2007，86，2： | 1 |
| 15 | ESR DETECTION OF ACTIVE INTERMEDIATES PRODUCED BY VIOLOGENMEDIATED REDUCTION OF SUBSTITUTED NITROARENES显示文摘ESRDETECTIONOFACTIVEINTERMEDIATESPRODUCEDBYVIOLOGENMEDIATEDREDUCTIONOFSUBSTITUTEDNITROARENES￥FuTianLIU;LingJunBAI;HongLIandBi... | Liu, FT Bai, LJ Li, H He, BL | 1996 | Chinese Chemical Letters1996，7，3： | 0 |
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