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| 1 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 2 | PIKA as an Adjuvant Enhances Specific Humoral and Cellular Immune Responses Following the Vaccination of Mice with HBsAg plus PIKA显示文摘An adjuvant is usually used to enhance the immune response induced by vaccines. The choice of adjuvant or immune enhancer determines the effectiveness of the immune response. Currently, aluminium (Alum, a generic term for salts of aluminium) is the only FDA-approved adjuvant. Alum predominantly induces the differentiation of Th2 cells and thus mediates an antibody immune response. Therefore, there is an urgent need for new adjuvants that enhance not only humoral but also cellular immune responses. In the present study, we demonstrates that PIKA (a stabilized dsRNA) as an adjuvant directly induces the activation and the proliferation of both B and NK cells in vitro. Injection of PIKA into mice results in the production of cytokines in vivo. In addition, the study demonstrates that PIKA promotes the maturation of bone marrow-derived dendritic cells (BMDCs) including up-regulation of the co-stimulatory molecules CD80, CD86 and CD40, and the induction of cytokines such as IL-12p70, IL-12p40 and IL-6. Importantly, after immunization of mice with HBsAg plus PIKA, the presence of PIKA enhances the titers of HBsAg-specific IgG and HBsAg-specific IFN-γ production. These results demonstrate that PIKA as an adjuvant can promote both humoral and cellular immune responses. These might have an implication in applying PIKA as an adjuvant to be used in the design and development of both therapeutic and preventive vaccines, and used in the clinical study. | Erxia Shen Li Li Lietao Li Lianqiang Feng Lin Lu Ziliang Yao Haixiang Lin Changyou Wu | 2007 | Cellular & Molecular Immunology2007，4，2： | 13 |
| 3 | Removing Endotoxin from Protein Solution by Chitosan Modified Affinity Membrane显示文摘Affinity membrane was prepared with chitosan immobilized on the hydrophile- modified poly(vinylidene fluoride) (PVDF) membrane. Fourier transform infrared spectroscopy (FTIR.) analysis indicated that the contents of —NH2 and —OH groups increased and fluoride decreased on the membrane surface after modification. Using this kind of affinity membrane, the effects of operation parameters such as pH, ionic strength and flow rate, on the amount of endotoxin removed were investigated. The results showed that the equilibrium adsorption capacity and the dissociation constant of the affinity membrane to endotoxin were 21.4 EU·mg-1 membrane and 0.50EU·ml-1, respectively, at pH 7.0 and ionic strength 0.2 mol·L-1. Adsorption appeared to follow a typical Langmuir adsorption isotherm. At pH 5.0, ionic strength of 0.2 mol·L-1, the removal rate of endotoxin from BSA solution with the chitosan affinity membrane was up to 88.6% (11.50 EU·mg-1 membrane), and the recovery of BSA was 93.4% (0.187mg·mg-1 membrane), while at pH 11.0, ionic strength of 0.2mol·L-1, the removal rate of endotoxin from lysozyme solution was 72.4% (9.92EU·mg-1 membrane), and the recovery of lysozyme was 92.3% (0.104 mg·mg-1 membrane). | SUN Haixiang(孙海翔) ZHANG Lin(张林) CHAI Hong(柴红) CHEN Huanlin(陈欢林) | 2005 | Chinese Journal of Chemical Engineering2005，13，4： | 5 |
| 4 | Updating preconditioner for iterative method in time domain simulation of power systems显示文摘The numerical solution of the differential-algebraic equations(DAEs) involved in time domain simulation(TDS) of power systems requires the solution of a sequence of large scale and sparse linear systems.The use of iterative methods such as the Krylov subspace method is imperative for the solution of these large and sparse linear systems.The motivation of the present work is to develop a new algorithm to efficiently precondition the whole sequence of linear systems involved in TDS.As an improvement of dishonest preconditioner(DP) strategy,updating preconditioner strategy(UP) is introduced to the field of TDS for the first time.The idea of updating preconditioner strategy is based on the fact that the matrices in sequence of the linearized systems are continuous and there is only a slight difference between two consecutive matrices.In order to make the linear system sequence in TDS suitable for UP strategy,a matrix transformation is applied to form a new linear sequence with a good shape for preconditioner updating.The algorithm proposed in this paper has been tested with 4 cases from real-life power systems in China.Results show that the proposed UP algorithm efficiently preconditions the sequence of linear systems and reduces 9%-61% the iteration count of the GMRES when compared with the DP method in all test cases.Numerical experiments also show the effectiveness of UP when combined with simple preconditioner reconstruction strategies. | WANG Ke XUE Wei LIN HaiXiang XU ShiMing ZHENG WeiMin | 2011 | Science China(Technological Sciences)2011，54，4： | 3 |
| 5 | Solving Sparse Least Squares Problemes with Preconditioned CGLS Method on Parallel Distributed Memory Computers 显示文摘 | Tianruo Yang and Haixiang Lin | 1999 | Parallel Algorithms and Applications1999，，13： | 1 |
| 6 | Delay-induced inward and outward spiral waves in oscillatory medium显示文摘 | HU Haixiang JI Lin LI Shuqian | 2008 | J ChemPhys2008，128，04： | 1 |
| 7 | Study of human-globulin adsorption capacity of PVDF hollow fiber affinity membranes containing different amino acid ligands显示文摘 | Sun Haixiang Zhang Lin Chai Hong | 2006 | Sep Purif Technol2006，48，： | 1 |
| 8 | Solving Sparse Least Squares Problemes with Preconditioned CGLS Method on Parallei Distributed Memory Computers显示文摘 | Tianruo Yang and Haixiang Lin | 1999 | Parallel Algorithms and Applications1999，13，： | 1 |
| 9 | Surface modification of poly(tetrafluoroethylene) films via plasma treatment and graft copolymerization of acrylic acid 显示文摘 | SUN Haixiang ZHANG Lin | 2006 | Desalination2006，192，1： | 1 |
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