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| 1 | Transformation of human amniotic epithelial cells into neuron-like cells in the microenvironment of traumatic brain injury in vivo and in vitro显示文摘Survival and differentiation of transplanted cells is closely related to the local microenvironment.The present study cultured human amniotic epithelial cells(HAECs) in a simulated microenvironment in vitro comprising RPMI 1640 culture medium and the solution extracted from injured brain tissues.Some HAECs were round,triangular in form or irregularly shaped,with extended neuron-like processes;some of the processes were interconnected,representing neuron-like morphology and some HAECs were microtubule-associated protein 2-positive.HAECs survived for at least 4 weeks following transplantation into the center and edges of the trauma focus with traumatic brain injury,and were microtubule-associated protein 2-positive.Moreover,the motor function of rat hind limbs was significantly improved. | Yi Lu Guozhen Hui Zhiyuan Wu Tianjin Liu Xunhe Ji Lihe Guo | 2011 | Neural Regeneration Research2011，6，10： | 10 |
| 2 | Quadrilateral isoparametric finite elements for plane elastic Cosserat bodies显示文摘4-node, 8-node and 8(4)-node quadrilateral plane isoparametric elements are used for the solution of bound ary value problems in linear isotropic Cosserat elasticity. The patch test is applied to validate the finite elements. Engineering problems of stress concentration around a circular hole in plane strain condition and mechanical behaviors of heterogeneous materials with rigid inclusions and pores are computed to test the accuracy and capability of these three types of finite elements. | Hongwu Zhang Hui Wang Guozhen Liu | 2005 | Acta Mechanica Sinica2005，21，4： | 8 |
| 3 | Brain-derived neurotrophic factor induces neuron-like cellular differentiation of mesenchymal stem cells derived from human umbilical cord blood cells in vitro显示文摘Human umbilical cord blood was collected from full-term deliveries scheduled for cesarean section.Mononuclear cells were isolated,amplified and induced as mesenchymal stem cells.Isolated mesenchymal stem cells tested positive for the marker CD29,CD44 and CD105 and negative for typical hematopoietic and endothelial markers.Following treatment with neural induction medium containing brain-derived neurotrophic factor for 7 days,the adherent cells exhibited neuron-like cellular morphology.Immunohistochemical staining and reverse transcription-PCR revealed that the induced mesenchymal stem cells expressed the markers for neuron-specific enolase and neurofilament.The results demonstrated that human umbilical cord blood-derived mesenchymal stem cells can differentiate into neuron-like cells induced by brain-derived neurotrophic factor in vitro. | Lei Chen Zhongguo Zhang Bing Chen Xiaozhi Liu Zhenlin Liu Hongliang Liu Gang Li Zhiguo Su Junfei Wang Guozhen Hui | 2011 | Neural Regeneration Research2011，6，13： | 8 |
| 4 | Monoamine alterations and rotational asymmetry in a rat model of Parkinson's disease following lateral ventricle transplantation of human amniotic epithelial cells显示文摘BACKGROUND: Human amniotic epithelial cells (HAECs) can differentiate into neurons, astrocytes,and oligodendrocytes. They biologically secrete many active neurotrophins and have the capacity tometabolize dopamine enzymes. These features underlie a theoretical basis for the treatment ofParkinson's disease (PD).OBJECTIVE: To investigate the survival and differentiation of transplanted HAECs in the lateralventricle of PD model rats, and to explore its effect on circling behavior, as well as levels ofdopamine (DA), the metabolite homovanillic acid, dihydroxyphenyl acetic acid,5-hydroxyindoleacetic acid, and 5-hydroxytryptamine in the striatum.DESIGN, TIME AND SETTING: A randomized, controlled, animal study was performed at theInstitute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, ChineseAcademy of Sciences, and Shanghai Celstar Institute of Biotechnology from May 2007 to December2008.MATERIALS: HAECs were derived from the placental chorion following caesarean delivery at theShanghai International Maternal and Child Health Hospital. 6-hydroxydopamine (6-OHDA), andmouse anti-human Vimentin monoclonal antibody were purchased from Sigma, USA; mouseanti-human nestin and tyrosine hydroxylase (TH) monoclonal antibodies were purchased fromChemicon, USA.METHODS: A total of 114 healthy, adult, Sprague Dawley rats were randomly assigned to twogroups: PD model [n = 90, stereotactic microinjection of 2 μL 6-OHDA (3.5 μg/μL) into the striatum]and control (n = 24, no treatment). The 51 successful PD model rats were randomly divided into 3subgroups (n = 17): HAEC, PBS, and model. The HAEC and PBS groups were respectively injectedwith 10 μL PBS solution containing 1 x 105/mL HAECs or 10 μL PBS into the lateral ventricle. Themodel group was not treated.MAIN OUTCOME MEASURES: TH protein expression in the striatum was evaluated byimmunohistochemistry 5 weeks after HAEC transplantation. At 10 weeks, HAEC survival in thelateral ventricle was investigated by immunofluorescent staining; differentiation of HAECs in thelateral and third ventricles was examined by TH immunohistochemistry; concentrations of DA,homovanillic acid, dihydroxyphenyl acetic acid, 5-hydroxyindoleacetic acid, and 5-hydroxytryptaminein the striatum, as well as DA concentration in the cerebrospinal fluid, were measured withhigh-performance liquid chromatography-electrochemical detection. Circling behavior of PD modelrats was consecutively observed for 10 weeks following intraperitoneal injection of amphetamine 1week after successful model establishment.RESULTS: In the HAEC group, the number of TH-positive cells significantly increased in thestriatum, and circling behavior significantly decreased, compared with the PBS and model groups(P < 0.01). In addition, monoamine concentrations in the striatum, as well as DA concentrations inthe cerebrospinal fluid, significantly increased, compared with the PBS group (P < 0.05-0.01 ).Moreover, a large number of nestin-, vimentin-, and TH-positive cells were observed in the lateraland third ventricles following HAEC injection. CONCLUSION: HAECs survived for 10 weeks with no overgrowth following transplantation into thelateral ventricle of PD model rats. Moreover, the cells differentiated into dopaminergic neurons,which increased DA secretion. HAEC transplantation improved cycling behavior in PD model rats. | Shouru Xue Xinxin Yang Wanli Dong Guozhen Hui Lihe GUO | 2009 | Neural Regeneration Research2009，4，12： | 6 |
| 5 | Transcriptional Characteristics of Xa21-mediated Defense Responses in Rice显示文摘细菌的老家,由 Xanthomonas oryzae pv 引起了。oryzae (Xoo ) ,是米饭的最破坏的细菌的疾病。克隆的米饭基因 Xa21 授与抵抗到许多 Xoo 赛跑。为了识别基因,在 调停Xa21 的免疫包含了,米饭的整个染色体的 oligonucleotide microarray 被用来在 0 点介绍在不兼容的相互作用和嘲笑处理之间的米饭基因的表示, 4 , 8 , 24 , 72 和 120 h 柱子接种( hpi )或在在 4 hpi 的不兼容、兼容的相互作用之间分别地。441 差别的一个总数表示了基因,指定了为 XDG (Xa21 调停了差别表示了基因) ,被识别。基于他们的功能的注解, XDG 被分到 14 个范畴,包括防卫相关,发信号, transcriptional 管理者。大多数防卫相关的基因属于致病相关的基因家庭,它在 72 和 120 hpi 戏剧性地被导致。有趣地,大多数发信号和 transcriptional 管理者基因是在 4 和 8 hpi 的 downregulated,建议细胞的发信号的那条否定规定可以起在调停 Xa21 的防卫反应的一个作用。在 Xa21- 和其它 R 调停基因的防卫系统之间的表示侧面的比较揭示了有趣的普通回答。有支持证据的代表性的 XDG 也被讨论。 | Qiang Gan Hui Bai Xianfeng Zhao Yong Tao Haipan Zeng Yuning Han Wenyuan Song Lihuang Zhu Guozhen Liu | 2011 | Journal of Integrative Plant Biology2011，53，4： | 5 |
| 6 | An analytic solution describing the visco-elastic deformation of coal pillars in room and pillar mine显示文摘Coal pillar deformation is typically nonlinear and time-dependent. The accurate prediction of this defor- mation has a vital importance for the successful implementation of mining techniques. These methods have proven very important as a way to excavate coal resources from under buildings, railways, or water bodies. Elastic and visco-elastic theory are employed with a Maxwell model to formulate an analytic solution for displacement of coal pillars in room and pillar mine. These results show that the visco-elastic solution adequately predicts the coal pillar deformation over time. We conclude that the visco-elastic solution can predict the coal pillar and roadway displacement from the measured geological parameters of the conditions in situ. Furthermore, this method would be useful for mine design, coal pillar support optimization, ground subsidence prediction, and coal pillar stability analysis. | Li Qiang XU Hui BU Wankui Zhao Guozhen | 2011 | Mining Science and Technology2011，21，6： | 5 |
| 7 | Human amniotic epithelial cells express specific markers of nerve cells and migrate along the nerve fibers in the corpus callosum显示文摘Human amniotic epithelial cells were isolated from a piece of fresh amnion. Using immunocytochemical methods, we investigated the expression of neuronal phenotypes (microtubule-associated protein-2, glial fibrillary acidic protein and nestin) in human amniotic epithelial cells. The conditioned medium of human amniotic epithelial cells promoted the growth and proliferation of rat glial cells cultured in vitro, and this effect was dose-dependent. Human amniotic epithelial cells were further transplanted into the corpus striatum of healthy adult rats and the grafted cells could integrate with the host and migrate 1-2 mm along the nerve fibers in corpus callosum. Our experimental findings indicate that human amniotic epithelial cells may be a new kind of seed cells for use in neurograft. | Zhiyuan Wu Guozhen Hui Yi Lu Tianjin Liu Qin Huang Lihe Guo | 2012 | Neural Regeneration Research2012，7，1： | 3 |
| 8 | On-chip structure-switching aptamer-modified magnetic nanobeads for the continuous monitoring of interferon-gamma ex vivo显示文摘Cytokines are cell signaling molecules that indicate the health status of the body.In this study,we developed a microfluidic device integrated with structure-switching aptamers capable of continuously tracking the concentration of the cytokine interferon gamma(IFN-γ)in cell culture medium and blood serum.First,a ferrocene(Fc)-labeled structure-switching signaling aptamer with a hairpin structure targeting IFN-γwas immobilized on magnetic nanobeads by the strongest noncovalent interactions between streptavidin and biotin.The aptamer-modified magnetic nanobeads were trapped on a customized microfluidic chip by a magnetic field to form the sensing interface.The binding of IFN-γcould trigger the hairpin structure of the aptamer to unfold,pushing Fc redox molecules away from the sensing interface and consequently switching off the electrochemical signal.The change in the redox current of Fc was quantitatively related to the concentration of IFN-γin a linear range of 10–500 pg mL^(−1)and with the lowest detection limit of 6 pgmL^(−1).This microfluidic device was specific to IFN-γin the presence of overabundant serum proteins and allowed the continuous monitoring of IFN-γwithout adding exogenous reagents.It provided a universal point-of-care biosensing platform for the real-time detection of a spectrum of analytes. | Guozhen Liu Chaomin Cao Shengnan Ni Shilun Feng Hui Wei | 2019 | Microsystems & Nanoengineering2019，5，1： | 3 |
| 9 | Therapeutic effects of human amniotic epithelial cell transplantation on double-transgenic mice co-expressing APPswe and PS1ΔE9-deleted genes显示文摘Human amniotic epithelial cells (HAECs), which exhibit characteristics of embryonic and pluripotent stem cells, could be utilized for cell therapy without legal or ethical problems. Double-transgenic (TG) mice (n=20) and wild-type (WT) mice (n=20) were randomly assigned to two groups, respectively. The transplantation group was treated with HAECs and the control group with PBS. A six-radial arm water maze was used to assess spatial memory. Immunofluorescence was utilized to track HAEC survival. Immunohistochemistry was used to determine octamer-binding protein 4 (oct-4) and nanog expression in the HAECs. High-performance liquid chromatography (HPLC) was used to measure acetylcholine levels in the hippocampus. The density of cholinergic neurons in the basal forebrain and nerve fibers in the hippocampus was measured following acetylcholinesterase staining. Results showed that transplanted HAECs survived for at least eight weeks and migrated to the third ventricle without immune rejection. Graft HAECs also expressed the specific stem cell markers oct-4 and nanog. Compared with the control group, HAEC transplantation significantly ameliorated spatial memory deficits in TG mice, as well as increased acetylcholine levels and the number of hippocampal cholinergic neurites. Intracerebroventricular HAEC transplantation improved spatial memory in double-TG mice, and results suggested that increased acetylcholine levels in the hippocampus, released by surviving cholinergic neurites, were responsible for this improvement. | XUE ShouRu CHEN ChongFang DONG WanLi HUI GuoZhen LIU TianJun GUO Lille | 2012 | Science China(Life Sciences)2012，55，2： | 3 |
| 10 | Blockchain-Based Data Sharing System for AI-Powered Network Operations显示文摘The explosive development of mobile communications and networking has led to the creation of an extremely complex system,which is difficult to manage.Hence,we propose an AI-powered network framework that uses AI technologies to operate the network automatically.However,due to the separation between different mobile network operators,data barriers between diverse operators become bottlenecks to exploit the full power of AI.In this paper,we establish a mutual trust data sharing framework to break these data barriers.The framework is based on the distributed and temper-proof attributes of blockchain.We implement a prototype based on Hyperledger Fabric.The proposed system combines supervision and fine-grained data access control based on smart contracts,which provides a secure and trustless environment for data sharing.We further compare our system with existing data sharing schemes,and we find that our system provides a better functionality. | Guozhen Zhang Tong Li Yong Li Pan Hui Depeng Jin | 2018 | Journal of Communications and Information Networks2018，3，3： | 2 |
| 11 | MXene quantum dot within natural 3D watermelon peel matrix for biocompatible flexible sensing platform显示文摘Environmentally friendly biomimetic materials with good deformability,high pressure-sensitive performance,and excellent biocompatibility are highly attractive for health monitoring,but to simultaneously meet these requirements is a formidable challenge.In this study,biocompatible MXene quantum dot(MQD)/watermelon peel(WMP)aerogels were obtained by immersing freeze-dried fresh watermelon peel into the quantum dot dispersion.The resulting bio-aerogels with a three-dimensional(3D)porous network structure exhibited a low in elasticity modulus(0.03 MPa)and limit of detection(0.4 Pa)and it showed biocompatibility.With a maximum pressure-sensitive response of 323 kPa^(-1),the 3D porous MQD/WMP aerogels exhibited good stability.In addition,the sensing signals could be displayed on mobile phones through a Bluetooth module to monitor human motion(pulse,sound,and walking)in real time.More importantly,the MQD/WMP aerogels exhibited excellent biocompatibility in a cytotoxicity test,thus decreasing the safety risk when they are applied to human skin.The finding in this study will facilitate the fabrication of high-performance biomimetic MXene active matrices,which are derived from natural biological materials,for flexible electronics. | Jinzi Sun Hui Du Zhaojun Chen Lili Wang Guozhen Shen | 2022 | Nano Research2022，15，4： | 1 |
| 12 | Characterization and functional analysis of the genes encoding 1-deoxy- d -xylulose-5-phosphate reductoisomerase and 1-deoxy- d -xylulose-5-phosphate synthase, the two enzymes in the MEP pathway, from Amomum villosum Lour显示文摘 | Jinfen Yang Megha Nath Adhikari Hui Liu Hui Xu Guozhen He Ruoting Zhan Jieshu Wei Weiwen Chen | 2012 | Molecular Biology Reports2012，，8： | 1 |
| 13 | YBa_(2)Cu_(3)O_(7-x) Superconducting Thin Films Grown on CaNdA1O_(4)(100) Substrates显示文摘We report the fabrication of high temperature superconducting YBa_(2)Cu_(3)O_(7-x) thin films on CaNdA1O_(4)(100) substrate by pulsed laser deposition.Films have excellent features.X-ray diffraction patterns show that thin films are epitaxial with c-axis orientation normal to the surface of su bstrate.The four-terminal measurements indicate the zero resistance temperature of 92.1 K,and transition width of 0.7K,the critical current density of 3×10^(6)A/cm^(2)at 77K and under zero magnetic field. | XU Shifa ZHOU Yueliang LÜHuibin CUI Dafu CHEN Zhenghao YANG Guozhen TIAN Yongjun LIU Junzheng LI Lin CHANG Yingchuan HOU Desen FAN Hui ZHANG Zuren | 1994 | Chinese Physics Letters1994，11，5： | 0 |
| 14 | Upregulation of stromal cell-derived factor-1 alpha/CXCR4 axis-induced migration of human neural progenitors by tumor necrosis factor-alpha and interleukin-8显示文摘BACKGROUND:Studies of several animal models of central nervous system diseases have shown that neural progenitor cells(NPCs) can migrate to injured tissues.Stromal cell-derived factor 1 alpha (SDF-1α),and its primary physiological receptor CXCR4,have been shown to contribute to this process. OBJECTIVE:To investigate migration efficacy of human NPCs toward a SDF-1αgradient,and the regulatory roles of tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) in SDF-1α/CXCR4 axis-induced migration of NPCs. DESIGN,TIME AND SETTING:An in vitro,randomized,controlled,cellular and molecular biology study was performed at the Laboratory of Department of Cell Biology,Medical College of Soochow University between October 2005 and November 2007. MATERIALS:SDF-1αand mouse anti-human CXCR4 fusion antibody were purchased from R&D Systems,USA.TNF-αwas purchased from Biomyx Technology,USA and IL-8 was kindly provided by the Biotechnology Research Institute of Soochow University. METHODS:NPCs isolated from forebrain tissue of 9 to 10-week-old human fetuses were cultured in vitro.The cells were incubated with 0,20,and 40 ng/mL TNF-α,or 0,20,and 40 ng/mL IL-8,for 48 hours prior to migration assay.For antibody-blocking experiments,cells were further pretreated with 0,20,and 40μg/mL mouse anti-human CXCR4 fusion antibody for 2 hours.Subsequently,the transwell assay and CXCR4 blockade experiments were performed to evaluate migration of human NPCs toward a SDF-1αgradient.Serum-free culture medium without SDF-1αserved as the negative control. MAIN OUTCOME MEASURES:The transwell assay was performed to evaluate migration of human NPCs toward a SDF-1αgradient,which was blocked by fusion antibody against CXCR4.In addition, CXCR4 expression in human NPCs stimulated by TNF-αand IL-8 was measured by flow cytometry. RESULTS:Results from the transwell assay demonstrated that SDF-1αwas a strong chemoattractant for human NPCs(P<0.01),and 20 ng/mL produced the highest levels of migration. Anti-human CXCR4 fusion antibody significantly blocked the chemotactic effect(P<0.05).Flow cytometry results showed that treatment with TNF-αand IL-8 resulted in increased CXCR4 expression and greater chemotaxis efficiency of NPCs towards SDF-1α(P<0.01). CONCLUSION:These results demonstrated that SDF-1αsignificantly attracted NPCs in vitro,and neutralizing anti-CXCR4 antibody could block part of this chemotactic function.TNF-αand IL-8 increased chemotaxis efficiency of NPCs towards the SDF-1αgradient by upregulating CXCR4 expression in NPCs. | Jing Qu Hongtao Zhang Guozhen Hui Xueguang Zhang Huanxiang Zhang | 2009 | Neural Regeneration Research2009，4，11： | 0 |
| 15 | Cerebrospinal fluid used as culture medium prior to autologous olfactory ensheathing cell transplantation显示文摘BACKGROUND: Cerebrospinal fluid can be an inducer for neural stem cells in vitro, but few studies employ cerebrospinal fluid to culture olfactory ensheathing cells. OBJECTIVE: To investigate the growth of nasal mucosa olfactory ensheathing cells in normal cerebrospinal fluid, and to analyze the feasibility of cerebrospinal fluid for culturing olfactory ensheathing cells used for transplantation. DESIGN, TIME AND SETTING: A completely randomized, block design study was performed at the Cell Laboratory, Wuxi Third People's Hospital, and Jiangsu Institute of Parasitic Diseases, China, in August 2008. MATERIALS: Dulbecco's modified Eagle's medium/F12 (DMEM/F12) and fetal bovine serum (Gibco BRL, USA), mouse anti-rat P75 monoclonal antibody and rabbit anti-glial fibrillary acidic protein polyclonal anti body (Santa Cruz Biotechnology, USA), mouse anti-rat myelin basic protein monoclonal antibody (Cymbus, UK), mouse anti-rat microtubule-associated protein-2 monoclonal antibody (Transduction Laboratories, USA), FITC conjugated rabbit anti-mouse monoclonal antibody (Boster, China), TRITC conjugated goat anti-rabbit monoclonal antibody (Sigma, USA) were used. METHODS: Nasal mucosa olfactory ensheathing cells were separately incubated in DMEM/F12, cerebrospinal fluid, and changing DMEM/F12 into cerebrospinal fluid. Adult female Sprague Dawley rat models of spinal hemisection were established. Nerve injury was repaired by transplantation of nasal mucosa olfactory ensheathing cells cultured in cerebrospinal fluid or DMEM/F12. MAIN OUTCOME MEASURES: The proliferative ability of olfactory ensheathing cells cultured in cerebrospinal fluid was determined by a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay. The morphology and purity of olfactory ensheathing cells were detected using immunohistochemistry. Animal behavior was evaluated by the Basso, Beattie and Bresnahan locomotor rating scale. Morphological repair was assessed by a horseradish peroxidase-tetramethylbenzidine retrograde tracer technique and immunohistochemistry. RESULTS: Changing from DMEM/F12 to cerebrospinal fluid did not change overall culture morphology and purity on day 14. These cells also contributed to myelinization and the conduction velocity of regenerated axons, and improved motor abilities of denervated muscle fibers in rats with spinal cord injury. The recovery of behavioral function and neuronal regeneration was similar in the two groups. CONCLUSION: Cerebrospinal fluid culture prior to autologous olfactory ensheathing cell transplantation is feasible for clinical use. | Weijiang Wu Qing Lan Hua Lu Aihua Zhu Yunzhao Jiang Ge Chen Guozhen Hui | 2010 | Neural Regeneration Research2010，5，1： | 0 |
| 16 | Preface to the Special Issue on Flexible Materials and Structures for Bioengineering,Sensing,and Energy Applications显示文摘Human body with curved and soft interfaces requests advanced flexible materials and structures for the interaction with organs and signal collection from targets in applications such as bioengineering and diagnostic devices.Among them,it is highly demanded to achieve creative design in flexible materials and structures with great stretchable capability for required applications.To this end,both inorganic and organic materials could be adopted and designed with assembly and self-assembly methods for flexible electronics and electrodes.Soft or flexible materials and structures inspired by nature can lead to highly conformal contacts between devices and the human body.These approaches hold great potential for applications in flexible electronics,medical imaging technology and portable disease diagnostics.Novel strategy on related sensors/actuator and energy storage/generation devices could overcome certain limitations on flexible materials engineering and thus advance the field as well.All these methods would deliver a profound impact to our future intelligent society. | Yongfeng Mei Wei Gao Hui Fang Yuan Lin Guozhen Shen | 2020 | Journal of Semiconductors2020，41，4： | 0 |
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