| 1 | Multiscale label-free volumetric holographic histopathology of thick-tissue slides with subcellular resolution显示文摘Histopathology relies upon the staining and sectioning of biological tissues,which can be laborious and may cause artifacts and distort tissues.We develop label-free volumetric imaging of thick-tissue slides,exploiting refractive index distributions as intrinsic imaging contrast.The present method systematically exploits label-free quantitative phase imaging techniques,volumetric reconstruction of intrinsic refractive index distributions in tissues,and numerical algorithms for the seamless stitching of multiple three-dimensional tomograms and for reducing scattering-induced image distortion.We demonstrated label-free volumetric imaging of thick tissues with the field of view of 2 mm×1.75 mm×0.2 mm with a spatial resolution of 170 nm×170 nm×1400 nm.The number of optical modes,calculated as the reconstructed volume divided by the size of the point spread function,was∼20 giga voxels.We have also demonstrated that different tumor types and a variety of precursor lesions and pathologies can be visualized with the present method. | Herve Hugonnet Yeon Wook Kim Moosung Lee Seungwoo Shin Ralph H.Hruban Seung-Mo Hong YongKeun Park | 2021 | Advanced Photonics2021，3，2： | 5 |
您所在的机构暂未开通此部分的功能，请联系管理员