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| 1 | Concurrent removal of intravitreal lens fragments after phacoemulsification with pars plana vitrectomy prevents development of retinal detachment显示文摘AIM: To evaluate the outcomes of 'concurrent vitrectomy' to retrieve dislocated lens fragment during phacoemulsification.METHODS: In a retrospective, observational case series, data of patients who underwent 'concurrent' pars plana vitrectomy(PPV) for dislocated lens fragments between the period 2000 and 2008 were reviewed. Data collected included patient demographics, pre-operative visual acuity, intra-operative occurrence of retinal breaks, duration of follow up, post-operative intraocular pressure, final best-corrected visual acuity(BCVA),presence of cystoid macular edema(CME) and occurrence of rhegmatogenous retinal detachment(RRD).· RESULTS: A total of 58 eyes of 58 patients were included in the study. At 12 mo the mean postoperative BCVA was log MAR 0.17(20/30) with a range of log MAR0 to 0.69(20/20 to 20/100), with 96.6%(56/58) of patients showing post- operative improvement in visual acuity(P =0.005). None of the patients developed postoperative retinal detachment, endophthalmitis or non-resolving uveitis at 12 mo.CONCLUSION: Our study results suggest concurrent PPV for retained lens fragments after cataract surgery is beneficial and may decrease the risk of glaucoma and prevent development of RRD. | Kakarla V Chalam Ravi K Murthy Joshua C Priluck Vijay Khetpal Shailesh K Gupta | 2015 | International Journal of Ophthalmology(English edition)2015，8，1： | 4 |
| 2 | Histone deacetylase inhibitor pre-treatment enhances the efficacy of DNA-interacting chemotherapeutic drugs in gastric cancer显示文摘BACKGROUND The prognosis of gastric cancer continues to remain poor,and epigenetic drugs like histone deacetylase inhibitors(HDACi)have been envisaged as potential therapeutic agents.Nevertheless,clinical trials are facing issues with toxicity and efficacy against solid tumors,which may be partly due to the lack of patient stratification for effective treatments.To study the need of patient stratification before HDACi treatment,and the efficacy of pre-treatment of HDACi as a chemotherapeutic drug sensitizer.METHODS The expression activity of class 1 HDACs and histone acetylation was examined in human gastric cancer cells and tissues.The potential combinatorial regime of HDACi and chemotherapy drugs was defined on the basis of observed drug binding assays,chromatin remodeling and cell death.RESULTS In the present study,the data suggest that the differential increase in HDAC activity and the expression of class 1 HDACs are associated with hypoacetylation of histone proteins in tumors compared to normal adjacent mucosa tissue samples of gastric cancer.The data highlights for the first time that pretreatment of HDACi results in an increased amount of DNA-bound drugs associated with enhanced histone acetylation,chromatin relaxation and cell cycle arrest.Fraction-affected plots and combination index-based analysis show that pre-HDACi chemo drug combinatorial regimes,including valproic acid with cisplatin or oxaliplatin and trichostatin A with epirubicin,exhibit synergism with maximum cytotoxic potential due to higher cell death at low combined doses in gastric cancer cell lines.CONCLUSION Expression or activity of class 1 HDACs among gastric cancer patients present an effective approach for patient stratification.Furthermore,HDACi therapy in pretreatment regimes is more effective with chemotherapy drugs,and may aid in predicting individual patient prognosis. | Ramchandra Vigay Amnekar Shafqat Ali Khan Mudasir Rashid Bharat Khade Rahul Thorat Poonam Gera Shailesh V Shrikhande Duane T Smoot Hassan Ashktorab Sanjay Gupta | 2020 | World Journal of Gastroenterology2020，26，6： | 3 |
| 3 | Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma显示文摘AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P<0.001)and western blotting(1.92±0.26 vs 2.88±0.32;P<0.01).IHC revealed thatβ-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,P<0.001),as well as,in tumor tissues(2.79±0.24 vs6.71±0.14,P<0.001).Further,comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,P<0.01).Moreover,combined expression in epithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,P<0.05)in tumor tissues.In addition,univariate analysis showed a positive correlation ofβ-actin level of inflammatory cells with tumor grade(P<0.05)while epithelial cells exhibited negative correlation(P>0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade. | Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta | 2014 | World Journal of Gastroenterology2014，20，34： | 2 |
| 4 | Morpho-anatomical and physicochemical studies of Fumaria indica(Hausskn.)Pugsley显示文摘Objective:To study morpho-anatomical characters and physicochemical analysis of Fumaria indica(F.indica)(Hausskn.)Pugsley,(Fumariaceae),an important medicinal plant used extensively for treating a variety of ailments in various system of indigenous medicine.Methods:Evaluation of the different parts of the plant was carried out to determine the morphoanatomical,physicochemical,phytochemical and HPTLC fingerprinting profile of F.indica and other WHO recommended methods were performed for standardization.Results:Morphoanatomical studies showed compound and pinnatifid leaf,4 to 6 cm in length,linear and oblong in shape and anomocytic arrangement of stomata,thin walled parenchymatous cells,scattered,sclerenchymatous,capped vascular bundles and radiating medullary rays.Physicochemical studies showed foreign matter 0.2%,loss on drying 6.8%,total ash 16.77%,alcohol and water soluble extractives 8.92%and 20.26%,respectively,sugar 17.75%,starch 22.97%and tannins 2.37%.Phytochemical evaluation revealed the presence of carbohydrate,alkaloids,flavonoids,saponins,tannins and sterol.Thin layer chromatography was carried out with different solvents and the best solvent system was chloroform and methanol in 80:20 ratio and revealed 12 spots with different R_f value under UV light 366λ.Conclusions:The results of the study can serve as a valuable source of information and provide suitable standards for identification of this plant material for future investigations and applications. | Prakash Chandra Gupta Ch V Rao | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，10： | 2 |
| 5 | A review on ethnobotany,phytochemistry and pharmacology of Fumaria indica(Fumitory)显示文摘Fumaria indica(Hausskn.) Pugsley(Fumariaceae),known as 'Fumitory',is an annual herb found as a common weed all over the plains of India and Pakistan.The whole plant is widely used in traditional and folkloric systems of medicine.In traditional systems of medicine,the plant is reputed for its anthelmintic,diuretic,diaphoretic,laxative,cholagoguc,stomachic and sedative activities and is used to purify blood and in liver obstruction in ethnopharmacology. The whole plant is ascribed to possess medicinal virtues in Ayurvedic and Unani systems of medicine and is also used in preparation of important Ayurvedic medicinal preparations and polyhedral liver formulations.The review reveals that phytochemical constituents of wide range have been separated from the plants and it possesses important pharmacological activities like smooth muscle relaxant,spasmogenic and spasmolytic,analgesic,anti-inflammatory, neuropharmacological and antibacterial activities.The separation of hepatoprotective and antifungal constituents from this plant was also reported newly.This review highlights the traditional,ethnobotanical,phytochemical,pharmacological information available on Fumaria indica,which might be helpful for scientists and researchers to find out new chemical entities responsible for its claimed traditional uses. | Prakash Chandra Gupta Nisha Sharma Ch V Rao | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，8： | 2 |
| 6 | Axial strain enhances osteotomy repair with a concomitant increase in connexin43 expression显示文摘The mechanical environment is known to influence fracture healing. We speculated that connexin43(Cx43) gap junctions, which impact skeletal homeostasis, fracture healing and the osteogenic response to mechanical load,may play a role in mediating the response of the healing bone to mechanical strain. Here, we used an established rat fracture model, which uses a 2 mm osteotomy gap stabilized by an external fixator, to examine the impact of various cyclical axial loading protocols(2%, 10%, and 30% strain) on osteotomy healing. We examined the presence of Cx43 in the osteotomy-healing environment and assessed how mechanical strain modulates Cx43 expression patterns in the callus. We demonstrated that increased cyclical axial strain results in increased radiographic and histologic bone formation. In addition, we show by immunohistochemistry that Cx43 is abundantly expressed in the healing callus, with the expression most robust in samples exposed to increased cyclical axial strain. These data are consistent with the concept that an increase in Cx43 expression by mechanical load may be part of the mechanisms by which mechanical forces enhances fracture healing. | Rishi R Gupta Hyunchul Kim Yu-Kwan Chan Carla Hebert Leah Gitajn David J Yoo Robert V O’Toole Adam H Hsieh Joseph P Stains | 2015 | Bone Research2015，3，2： | 2 |
| 7 | Wavelet -based characterization of design ground motions 显示文摘 | Mukherjee S Gupta V K | 2002 | Earthquake Engineering and Structural Dynamics2002，31，5： | 1 |
| 8 | Removal of lead and chromium from wastewater using bagasse fly ash: a sugar industry waste 显示文摘 | Gupta V K Ali Imran | 2004 | Journal of Colloid and Interface Science2004，271，2： | 1 |
| 9 | Comparative analysis of genetic diversity among Indian populations of Scirpophaga incertulas by ISSR-PCR and RAPD-PCR显示文摘 | Kumar L S Sawant A S Gupta V S | 2001 | Biochem Genet2001，39，： | 1 |
| 10 | 显示文摘 | Gupta V Miura N | 2007 | Electro Chemistry2007，75，8： | 1 |
| 11 | Influence of zinc on calcium magnesium, sodium, potassium and phosphorus nutrition of soybeam(Glyeine max) in sodie soils 显示文摘 | Gupta S P Gupta V K | 1984 | Indian Journal of Ecology1984，11，2： | 1 |
| 12 | Nutrition of maize as Influenced by zinc and nitrogen carriers 显示文摘 | Gupta V K and Patalia B S | 1995 | J Indian Soc Sci1995，41，1： | 1 |
| 13 | A contribution to the geology of Northeastern Kumaun, with special reference to the Hercynian gap in Tethys Himalaya显示文摘 | Valdiya K S Gupta V J | 1972 | Himalaya Geology1972，2，： | 1 |
| 14 | Vascular events associated with alpha interferon therapy显示文摘 | AL-Zahrani H Gupta V Minden M D | 2003 | Leuk Lymphoma2003，44，： | 1 |
| 15 | Knowledge flows and the structure of control within multinational corporations显示文摘 | Gupta A K Govindarajan V | 1991 | Academy of Management Review1991，16，4： | 1 |
| 16 | A Representation of an IUH from Geomorphology显示文摘 | V K Gupta | 1980 | Water Resources Research1980，16，5： | 1 |
| 17 | Evolution approach for effective and efficient global minimization显示文摘 | DUAN Q Y GUPTA V K SOROOSHIAN S S | 1993 | Journal of Optimization Theory1993，76，3： | 1 |
| 18 | Probabilistic assessment of financial losses caused by interruptions and voltage sags: part Ⅰ-the methodology显示文摘 | Milanovic J V Gupta C P | 2006 | IEEE Trans on Power Develivery2006，21，2： | 1 |
| 19 | SRY-negative 46, XX male with normal genitals, complete masculinization and infertility显示文摘 | Rajender S Rajani V Gupta NJ | 2006 | Mol Hum Reprod2006，12，5： | 1 |
| 20 | Cultural Clusters: Methodology and Findings 显示文摘 | GUPTA V HANGES P J DORFMAN P | 2002 | Journal of World Business2002，，37： | 1 |
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