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| 1 | Isolation and characterization of H7N9 viruses from live poultry markets--Implication of the source of current H7N9 infection in humans显示文摘On March 31, 2013, the National Health and Family Planning Commission announced that human infections with a previously undescribed influenza A (H7N9) virus had occurred in Shanghai and Anhui Province, China. To investigate the possible origins of the H7N9 viruses causing these human infections, we collected 970 samples, including drinking water, soil, and cloacal and tracheal swabs of poultry from live poultry markets and poultry farms in Shanghai and Anhui Province. Twenty samples were positive for the H7N9 influenza virus. Notably, all 20 viruses were isolated from samples collected from live poultry markets in Shanghai. Phylogenetic analyses showed that the six internal genes of these novel human H7N9 viruses were derived from avian H9N2 viruses, but the ancestor of their HA and NA genes is uncertain. When we examined the phylogenetic relationship between the H7N9 isolates from live poultry markets and the viruses that caused the human infections, we found that they shared high homology across all eight gene segments. We thus identified the direct avian origin of the H7N9 influenza viruses that caused the human infections. Importantly, we observed that the H7N9 viruses isolated from humans had acquired critical mutations that made them more 'human-like'. It is therefore imperative to take strong measures to control the spread of H7N9 viruses in birds and humans to prevent further threats to human health. | SHI JianZhong DENG GuoHua LIU PeiHong ZHOU JinPing GUAN LiZheng LI WenHui LI XuYong GUO Jing WANG GuoJun FAN Jun WANG JinLiang LI YuanYuan JIANG YongPing LIU LiLing TIAN GuoBin LI ChengJun CHEN HuaLan | 2013 | Chinese Science Bulletin2013，58，16： | 85 |
| 2 | H7N9 virulent mutants detected in chickens in China pose an increased threat to humans显示文摘 | Jianzhong Shi Guohua Deng Huihui Kong Chunyang Gu Shujie Ma Xin Yin Xianying Zeng Pengfei Cui Yan Chen Huanliang Yang Xiaopeng Wan Xiurong Wang Liling Liu Pucheng Chen Yongping Jiang Jinxiong Liu Yuntao Guan Yasuo Suzuki Mei Li Zhiyuan Qu Lizheng Guan Jinkai Zang Wenli Gu Shuyu Han Yangming Song Yuzhen Hu Zeng Wang Linlin Gu Wenyu Yang Libin Liang Hongmei Bao Guobin Tian Yanbing Li Chuanling Qiao Li Jiang Chengjun Li Zhigao Bu Hualan Chen | 2017 | Cell Research2017，27，12： | 70 |
| 3 | Vaccination of poultry successfully eliminated human infection with H7N9 virus in China显示文摘The H7 N9 viruses that emerged in China in 2013 were nonpathogenic in chickens but mutated to a highly pathogenic form in early 2017 and caused severe disease outbreaks in chickens. The H7 N9 influenza viruses have caused five waves of human infection, with almost half of the total number of human cases(766 of 1,567) being reported in the fifth wave, raising concerns that even more human infections could occur in the sixth wave. In September 2017, an H5/H7 bivalent inactivated vaccine for chickens was introduced, and the H7 N9 virus isolation rate in poultry dropped by 93.3% after vaccination. More importantly,only three H7 N9 human cases were reported between October 1, 2017 and September 30, 2018, indicating that vaccination of poultry successfully eliminated human infection with H7 N9 virus. These facts emphasize that active control of animal disease is extremely important for zoonosis control and human health protection. | Xianying Zeng Guobin Tian Jianzhong Shi Guohua Deng Chengjun Li Hualan Chen | 2018 | Science China(Life Sciences)2018，61，12： | 37 |
| 4 | Effect of Root Architecture on Structural Stability and Erodibility of Topsoils during Concentrated Flow in Hilly Loess Plateau显示文摘Traditional vegetation techniques for the control of concentrated flow erosion are widely recognized, whereas only a few studies have experimentally investigated the impacts of belowground roots on the erodibility of topsoils in semi-arid areas. To quantify the effects of root architectures on soil erodibility and its relevant structural properties, simulated flow experiments were conducted at six-week intervals from 18 July to 20 October in 2012 in the hilly Loess Plateau. Five treatments were: 1) bare(control), 2) purple alfalfa(Medicago sativa), representing tap roots(T), 3) switchgrass(Panicum virgatum), representing fibrous roots(F), 4) purple alfalfa and switchgrass, representing both tap and fibrous roots(T + F), and 5) natural recovery(N). For each treatment, soil structural properties and root characteristics were measured at an interval of six weeks. Soil anti-scouribility was calculated. Results showed that grass planting slightly reduced soil bulk density, but increased soil aggregate content by 19.1%, 10.6%, 28.5%, and 41.2% in the treatments T, F, T + F, and N, respectively. Soil shear strength(cohesion and angle of internal friction(φ)) significantly increased after the grass was planted. As roots grew, soil cohesion increased by 115.2%–135.5%, while soil disintegration rate decreased by 39.0%–58.1% in the 21 th week compared with the recorded value in the 9th week. Meanwhile, root density and root surface area density increased by 64.0%–104.7% and 75.9%–157.1%, respectively. No significant differences in soil anti-scouribility were observed between the treatments of T and F or of T + F and N, but the treatments of T + F and N performed more effectively than T or F treatment alone in retarding concentrated flow. Soil aggregation and root surface-area density explained the observed soil anti-scouribility during concentrated flow well for the different treatments. This result proved that the restoration of natural vegetation might be the most appropriate strategy in soil reinforcement in the hilly Loess Plateau. | LI Qiang LIU Guobin ZHANG Zheng TUO Dengfeng XU Mingxiang | 2015 | Chinese Geographical Science2015，25，6： | 13 |
| 5 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 6 | Age-related Changes of Carbon Accumulation and Allocation in Plants and Soil of Black Locust Forest on Loess Plateau in Ansai County, Shaanxi Province of China显示文摘在高原生态系统吸引了的瓷器黄土的碳(C) 隐遁上的重新造林的效果很在最近的年里研究注意。黑蝗虫树(Robinia pseudoacacia L.) 在重新造林和水和土壤保存努力为他们的重要使用被珍视。这种森林类型越过黄土高原是普遍的,并且一定是任何在这个易碎的生态的区域为 C 隐遁努力计划的一个必要部件。在重新造林仍然保持不明确以后,看台的长期的效果在 C 累积和分配上变老。我们在黄土高原上检验了黑蝗虫森林(后来种的 5, 9, 20, 30, 38,和 56 年) 的年龄顺序在植物(树,灌木,牧草,和叶垃圾) 和土壤(0100 厘米) 评估 C 累积和分配。Allometric 方程为估计树部件的生物资源被开发(叶,树枝,茎没有吠叫,吠叫并且根) 与一个破坏采样方法。我们的结果证明那个黑蝗虫森林生态系统常常积累了 C,从 31.42 Mg C/ 哈(1 Mg = 10 < 啜 class= “ a-plus-plus ” > 6 g ) 在到在 38 年的 79.44 Mg C/ha 的 5 年。在旧森林阶段(38 ~ 56 年) ,在植物生物资源的 C 的数量显著地减少了(从 45.32 ~ 34.52 Mg C/ha ) 由于树的高死亡。然而,旧森林能在土壤连续地积累 C (从 33.66 ~ 41.00 Mg C/ha ) 。在灌木生物资源的 C 与看台年龄增加了,当在牧草层和叶垃圾的 C 股票是年龄无关的时。重新造林在森林土壤导致了 C 重新分配。C 存货与看台年龄常常增加了的表层土(020 厘米) 。然而,在 2030, 3050, 50100,和 20100 厘米层,在亚顶土壤的 C 存储是年龄无关的。这些结果建议作为一个时间的因素,继任在植被在地区性的 C 动力学在黑蝗虫森林里并且也在 C 累积和重新分配起一个关键作用。 | LI Taijun LIU Guobin | 2014 | Chinese Geographical Science2014，24，4： | 11 |
| 7 | Distribution and drug resistance of pathogenic bacteria isolated from cancer hospital in 2013显示文摘Objective: To understand distribution and drug resistance of pathogenic bacteria from a specialized cancer hospital in 2013 in order to provide a basis for rational clinical antimicrobial agents. Methods: Pathogenic bacteria identification and drug sensitivity tests were performed with a VITEK 2 compact automatic identification system and data were analyzed using WHONET5.6 software.Results: Of the 1,378 strains tested, 980 were Gram-negative bacilli, accounting for 71.1%, in which Klebsiella pneumonia, Escherichia coli and Pseudomonas aeruginosa were the dominant strains. We found 328 Gram-positive coccus, accounting for 23.8%, in which the amount of Staphylococcus aureus was the highest. We identified 46 fungi, accounting for 4.1%. According to the departmental distribution within the hospital, the surgical departments isolated the major strains, accounting for 49.7%. According to disease types, lung cancer, intestinal cancer and esophagus cancer were the top three, accounting for 20.9%, 17.3% and 14.2%, respectively. No strains were resistant to imipenem, ertapenem or vancomycin.Conclusions: Pathogenic bacteria isolated from the specialized cancer hospital have different resistance rates compared to commonly used antimicrobial agents; therefore antimicrobial agents to reduce the morbidity and mortality of infections should be used. | Linjuan Liu Qi Li Qingyun Zhang Guohong Wang Guobin Xu Ji Zhang | 2014 | Chinese Journal of Cancer Research2014，26，6： | 10 |
| 8 | Water in the Mogao Grottoes,China:where it comes from and how it is driven显示文摘The Dunhuang Mogao Grottoes in China was designated as a world heritage site by UNESCO in 1987 and is famous for its cultural relics. Water is the most active factor that harms the relics in the caves as it damages the grotto murals and painted sculptures. Thus, determining the water sources and driving forces of water movement is a key issue for protecting these cultural relics. These issues have troubled relics protectors for a long time. In this study, the authors chose a representative cave in the Mogao Grottoes and, by completely sealing the cave to make a closed system, measured the water vapor from the surrounding rock. This was accomplished by installing a condensation–dehumidification temperature–humidity control system for the collection of water vapor. The results show that there is continuous evaporation from the deep surrounding rock into the cave. The daily evaporation capacity is determined to be 1.02 g/(d?m2). The water sources and driving forces of water movement were further analyzed according to the character of the water evaporation and by monitoring the temperature and humidity of the surrounding rock. It was found that the water vapor in the cave derives from phreatic water. Moreover, the yearly fluctuation of temperature in the surrounding rock and geothermal forces are the basic powers responsible for driving phreatic evaporation. Under the action of the yearly temperature fluctuations, decomposition and combination of bound water acts as a 'pump' that drives phreatic water migration and evaporation. When the temperature rises, bound water decomposes and evaporates; and when it falls, the rock absorbs moisture. This causes the phreatic water to move from deep regions to shallow ones. Determining the source and dynamic foundation of the water provides a firm scientific basis for protecting the valuable cultural relics in the caves. | HongShou LI WanFu WANG HongTao ZHAN Fei QIU QingLin GUO GuoBin ZHANG | 2015 | Journal of Arid Land2015，7，1： | 10 |
| 9 | Fine Mapping of RppP25, a Southern Rust Resistance Gene in Maize显示文摘Southern rust (Puccinia polysora Underw.) is a major disease that can cause severe yield losses in maize (Zea mays L.). In our previous study, a major gene RppP25 that confers resistance to southern rust was identified in inbred line P25. Here, we report the fine mapping and candidate gene analysis of RppP25 from the near-isogenic line F939, which harbors RppP25 in the genetic background of the susceptible inbred line F349. The inheritance of resistance to southern rust was investigated in the BC1F1 and BC3F1 populations, which were derived from a cross between F939 and F349 (as the recurrent parent). The 1:1 segregation ratio of resistance to susceptible plants in these two populations indicated that the resistance is controlled by a single dominant gene. Ten markers, including three simple sequence repeat (SSR) markers and seven insertion/deletion (InDel) markers, were developed in the RppP25 region. RppP25 was delimited to an interval between P091 and M271, with an estimated length of 40 kb based on the physical map of B73. In this region, a candidate gene was identified that was predicted to encode a putative nucleotide-binding site leucine-rich repeat (NBS-LRR) protein. Two co-segregated markers will aid in pyramiding diverse southern rust resistance alleles into elite materials, and thereby improve southern rust resistance worldwide. | Panfeng Zhao Guobin Zhang Xiaojun Wu Na Li Dianyi Shi Dengfeng Zhang Chunfang Ji Mingliang Xu Shoucai Wang | 2013 | Journal of Integrative Plant Biology2013，55，5： | 5 |
| 10 | Recent Changes in Precipitation Extremes in the Heihe River Basin,Northwest China显示文摘Changes in rainfall extremes pose a serious and additional threat to water resources planning and management,natural and artificial oasis stability,and sustainable development in the fragile ecosystems of arid inland river basins. In this study,the trend and temporal variation of extreme precipitation are analyzed using daily precipitation datasets at 11 stations over the arid inland Heihe River basin in Northwest China from 1960 to 2011. Eight indices of extreme precipitation are studied.The results show statistically significant and large-magnitude increasing and decreasing trends for most indices,primarily in the Qilian Mountains and eastern Hexi Corridor. More frequent and intense rainfall extremes have occurred in the southern part of the desert area than in the northern portion. In general,the temporal variation in precipitation extremes has changed throughout the basin. Wet day precipitation and heavy precipitation days show statistically significant linear increasing trends and step changes in the Qilian Mountains and Hexi Corridor. Consecutive dry days have decreased obviously in the region in most years after approximately the late 1980 s,but meanwhile very long dry spells have increased,especially in the Hexi Corridor. The probability density function indicates that very long wet spells have increased in the Qilian Mountains. The East Asian summer monsoon index and western Pacific subtropical high intensity index possess strong and significant negative and positive correlations with rainfall extremes,respectively. Changes in land surface characteristics and the increase in water vapor in the wet season have also contributed to the changes in precipitation extremes over the river basin. | CHENG Aifang FENG Qi Guobin FU ZHANG Jiankai LI Zongxing HU Meng WANG Gang | 2015 | Advances in Atmospheric Sciences2015，32，10： | 5 |
| 11 | Application of the oblique ionogram as vertical ionogram显示文摘The vertical ionogram can provide the important ionospheric parameters, such as critical frequency, virtual height and electron density, for ionospheric research. The oblique ionosonde has the ability to detect the ionosphere over sea and other terrain where it is not practical to deploy vertical sounder and provide more ionograms with less transmitting and receiving devices. Therefore, the conversion of the oblique ionogram to vertical ionogram for obtaining the important ionospheric parameters is a very useful inversion technology. The experimental comparison between oblique and vertical detections was carried out in the equatorial ionospheric anomaly (EIA) region of south China on 25 and 26 August 2010. The oblique detecting path was from Wuhan to Shenzhen and the VI ionosonde was located in the midpoint of the oblique path. The oblique ionogram reversion results showed a small deviation of the critical frequency, minimum virtual height as well as the electron density profile of the ionospheric F layer, as compared with the real vertical observations. | CHEN Gang ZHAO ZhengYu ZHANG YuanNong YANG GuoBin ZHOU Chen HUANG Shuo LI Ting LI Ning SUN HengQing | 2012 | Science China(Technological Sciences)2012，55，5： | 5 |
| 12 | Reaction thermodynamics and kinetics on in situ Al_2O_3/Cu composites显示文摘The preferred internal oxidation of aluminum in Cu Al alloy was used to obtain in situ Al 2O 3/Cu composites. The reinforcement particles were mainly γ Al 2O 3, some θ Al 2O 3 and a little α Al 2O 3. Thermodynamics analyses show that the chemical reactions are 3Cu 2O+2Al=6Cu+Al 2O 3 or 3CuO+2Al=3Cu+Al 2O 3. A related equilibrium diagram was drawn. The experiments and investigation show that the formation rate of Al 2O 3 was controlled by the diffusion of oxygen in matrix. | Li Guobin(李国彬) 1, Wu Jianjun(武建军) 1, Guo Quanmei(郭全梅) 2, Jiang Yanfei(姜延飞) 1, Shen Yutian(申玉田) 1, Lei Tingquan(雷廷权) 2 1. Department of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, P.R.Chi | 1999 | 中国有色金属学会会刊:英文版1999，9，3： | 4 |
| 13 | Diagnostic value of negative enrichment and immune fluorescence in situ hybridization for intraperitoneal free cancer cells of gastric cancer显示文摘Objective:To explore the intraperitoneal free cancer cell(IFCC)detection value of negative enrichment and immune fluorescence in situ hybridization(NEimFISH)on chromosomes(CEN)8/17.Methods:To verify the reliability of NEimFISH,29 gastric cancer tumors,their adjacent tissues and greater omental tissues were tested.Our study then included 105 gastric cancer patients for IFCC.We defined patients as IFCC-positive if a signal was detected,regardless of the detailed cancer cell numbers.A comparison of clinicopathological features was conducted among IFCC groups.We also compared the diagnosis value and peritoneal recurrence predictive value among different detection methods.The comparison of IFCC number was also conducted among different groups.Results:A cutoff of 2.5 positive cells could distinguish all benign tissue samples and 97%of malignant tissue samples in our study.Compared to intestinal gastric cancer,patients with diffuse gastric cancer tended to have more IFCCs(6 vs.4,P=0.002).The IFCC counts were often higher in the lymphovascular invasion positive group than negative group(3 vs.1,P=0.022).All IFCC samples that were considered positive using conventional cytology were also found to be positive using NEimFISH.When compared to conventional cytology and paraffin pathology,NEimFISH had a higher IFCC positive rate(68.9%)and higher one-year peritoneal recurrence predictive value with area under the curve(AUC)of 0.922.Conclusions:Gastric cancer could be effectively diagnosed by NEimFISH.The IFCC number found using NEimFISH on CEN8/17 is closely associated with Lauren type and vascular invasion of cancer.NEimFISH is a reliable detection modality with a higher positive detection rate,higher one-year peritoneal recurrence predictive value and quantitative features for IFCC of gastric cancer. | Anqiang Wang Zhongwu Li Qian Wang Yali Bai Xin Ji Tao Fu Ke Ji Yanwen Xue Tingxu Han Xiaojiang Wu Ji Zhang Yingjie Yang Guobin Xu Zhaode Bu Jiafu Ji | 2019 | Chinese Journal of Cancer Research2019，31，6： | 3 |
| 14 | Influenza A virus use of BinCARD1 to facilitate the binding of viral NP to importinα7 is counteracted by TBK1-p62 axis-mediated autophagy显示文摘As a major component of the viral ribonucleoprotein(vRNP)complex in influenza A virus(IAV),nucleoprotein(NP)interacts with isoforms of importinαfamily members,leading to the import of itself and vRNP complex into the nucleus,a process pivotal in the replication cycle of IAV.In this study,we found that BinCARD1,an isoform of Bcl10-interacting protein with CARD(BinCARD),was leveraged by IAV for efficient viral replication.BinCARD1 promoted the nuclear import of the vRNP complex and newly synthesized NP and thus enhanced vRNP complex activity.Moreover,we found that BinCARD1 interacted with NP to promote NP binding to importinα7,an adaptor in the host nuclear import pathway.However,we also found that BinCARD1 promoted RIG-I-mediated innate immune signaling by mediating Lys63-linked polyubiquitination of TRAF3,and that TBK1 appeared to degrade BinCARD1.We showed that BinCARD1 was polyubiquitinated at residue K103 through a Lys63 linkage,which was recognized by the TBK1-p62 axis for autophagic degradation.Overall,our data demonstrate that IAV leverages BinCARD1 as an important host factor that promotes viral replication,and two mechanisms in the host defense system are triggered—innate immune signaling and autophagic degradation—to mitigate the promoting effect of BinCARD1 on the life cycle of IAV. | Xuyuan Wang Li Jiang Guangwen Wang Wenjun Shi Yuzhen Hu Bo Wang Xianying Zeng Guobin Tian Guohua Deng Jianzhong Shi Liling Liu Chengjun Li Hualan Chen | 2022 | Cellular & Molecular Immunology2022，19，10： | 3 |
| 15 | Reverse Microemulsion Synthesis and Characterization of Pd-Ag Bimetallic Alloy Catalysts Supported on Al_2O_3 for Acetylene Hydrogenation显示文摘Pd-Ag bimetallic alloy nanoparticles were synthesized by the reverse microemulsion method,and then deposited on Al2O3 to form the supported catalyst.The nanoparticles of Pd-Ag and Pd-Ag/Al2O3 samples were characterized by UV/Vis,HRTEM,EDX,XRD,and XPS.The test results indicated that Pd-Ag bimetallic alloy nanoparticles with a size of about 2 nm and a face-centered cubic(fcc) structure were formed in the measured area of microemulsion.The growth of nanoparticles was effectively limited within the droplet of micoremulsion.TEM image exhibited that the Pd-Ag alloy nanoparticles were well-dispersed on the Al2O3 support.The catalytic performance of various catalysts for selective hydrogenation of acetylene showed that a higher acetylene conversion and selectivity to ethylene upon acetylene hydrogenation was achieved on a nano-sized Pd-Ag bimetallic catalyst with a Pd/Ag alloy supported molar ratio of 1:1.5. | Wei Guobin Dai Wei Li Qian Cao Weiliang Zhang Jingchang | 2012 | China Petroleum Processing & Petrochemical Technology2012，14，3： | 3 |
| 16 | H7N9 virus infection triggers lethal cytokine storm by activating gasdermin E-mediated pyroptosis of lung alveolar epithelial cells显示文摘The H7N9 influenza virus emerged in China in 2013,causing more than 1560 human infections,39% of which were fatal.A ‘cytokine storm’ in the lungs of H7N9 patients has been linked to a poor prognosis and death;however,the underlying mechanism that triggers the cytokine storm is unknown.Here,we found that efficient replication of the H7N9 virus in mouse lungs activates gasdermin E(GSDME)-mediated pyroptosis in alveolar epithelial cells,and that the released cytosolic contents then trigger a cytokine storm.Knockout of Gsdme switched the manner of death of A549 and human primary alveolar epithelial cells from pyroptosis to apoptosis upon H7N9 virus infection,and Gsdme knockout mice survived H7N9 virus lethal infection.Our findings reveal that GSDME activation is a key and unique mechanism for the pulmonary cytokine storm and lethal outcome of H7N9 virus infection and thus opens a new door for the development of antivirals against the H7N9 virus. | Xiaopeng Wan Jiqing Li Yupeng Wang Xiaofei Yu Xijun He Jianzhong Shi Guohua Deng Xianying Zeng Guobin Tian Yanbing Li Yongping Jiang Yuntao Guan Chengjun Li Feng Shao Hualan Chen | 2022 | National Science Review2022，9，1： | 3 |
| 17 | Tim‐3/galectin‐9 signaling pathway mediates T‐cell dysfunction and predicts poor prognosis in patients with hepatitis B virus‐associated hepatocellular carcinoma显示文摘 | Hang Li Ke Wu Kaixiong Tao Libo Chen Qichang Zheng Xiaoming Lu Jun Liu Liang Shi Chuanqiao Liu Guobin Wang Weiping Zou | 2012 | Hepatology2012，，： | 2 |
| 18 | A chromosome-scale genome assembly of Artemisia argyi reveals unbiased subgenome evolution and key contributions of gene duplication to volatile terpenoid diversity显示文摘Artemisia argyi Le´vl.et Vant.,a perennial Artemisia herb with an intense fragrance,is widely used in traditional medicine in China and many other Asian countries.Here,we present a chromosome-scale genome assembly of A.argyi comprising 3.89 Gb assembled into 17 pseudochromosomes.Phylogenetic and comparative genomic analyses revealed that A.argyi underwent a recent lineage-specificwhole-genomeduplication(WGD)event after divergence fromArtemisia annua,resulting in two subgenomes.Wedeciphered the diploid ancestral genome of A.argyi,and unbiased subgenome evolution was observed.The recent WGD led to a large number of duplicated genes in the A.argyi genome.Expansion of the terpene synthase(TPS)gene family through various types of gene duplication may have greatly contributed to the diversity of volatile terpenoids in A.argyi.In particular,we identified a typical germacrene D synthase gene cluster within the expanded TPS gene family.The entire biosynthetic pathways of germacrenes,(+)-borneol,and(+)-camphor were elucidated in A.argyi.In addition,partial deletion of the amorpha-4,11-diene synthase(ADS)gene and loss of function of ADS homologs may have resulted in the lack of artemisinin production in A.argyi.Our study provides newinsights into the genome evolution of Artemisia and lays a foundation for further improvement of the quality of this important medicinal plant. | Hongyu Chen Miaoxian Guo Shuting Dong Xinling Wu Guobin Zhang Liu He Yuannian Jiao Shilin Chen Li Li Hongmei Luo | 2023 | Plant Communications2023，4，3： | 2 |
| 19 | In-situ Synthesis and Formation Mechanism ofTiN-Al_2O_3/Al Composite显示文摘The synthesis of TiN and Al2O3 by in situ injection of reactive nitrogen gas into molten Al alloys has been evaluated over the temperature range from 1000 to 1600℃. It is shown that TiN and Al2O3 can be formed in melt with nitrogen and surplus oxygen (in vacuum room of the induction furnace) as the reactive gases over 1000℃. Up to 2.1 wt pct Al2O3 and 5.2 wt pct of TiN in situ phases in an Al alloy has been formed in a range of size from 0.8 to 5 μm. The formation mechanism of TiN and Al2O3 is discussed in this paper. | Chunxiang CUP and Renjie WU (State Key Lab. for MMCs, Shanghai Jiaotong University, Shanghai 200030, China)Jianran YAN and Guobin LI (Hebei Polytechnic University Tianjin 300132, China) | 1998 | Journal of Materials Science & Technology1998，14，1： | 2 |
| 20 | Molecular cloning and expression patterns of the cholesterol side chain cleavage enzyme(CYP11A1) gene during the reproductive cycle in goose(Anas cygnoides)显示文摘Background: CYP11A1, a gene belonging to the family 11 of cytochrome P450, encodes a crucial steroidogenic enzyme that catalyzes the initial step in the production of all classes of steroids. Many studies show that CYP11A1 plays a role in ovary function. However, the role of CYP11A1 in goose reproductive cycle remains largely unknown.Results: In this study, full-length CYP11A1 c DNA of Zhedong goose was obtained using reverse transcription polymerase chain reaction(RT-PCR) and rapid amplification of c DNA ends(RACE). The c DNA consisted of a 96-base pair(bp) 5′untranslated region(UTR), a 179-bp 3′UTR and a 1509-bp open reading frame. The open reading frame encodes a putative 503 amino acid protein that shares high homology with CYP11A1 of other birds. The amino acid sequence possesses conserved domains of the P450 superfamily, which include the steroid-binding domain and the heme-binding region. Real-time quantitative polymerase chain reaction(q PCR) analysis revealed CYP11A1 mR NA was expressed ubiquitously in every Zhedong goose tissue analyzed, including the heart, liver, glandular stomach,lung, spleen, kidney, intestinum tenue, intestinum crassum, cerebrum, cerebellum, muscle, oviduct, pituitary,hypothalamus and ovary.. The relatively low levels of CYP11A1 m RNA were detected in pituitary, ovary and oviduct tissues at ovulation when compared with levels at oviposition. Interestingly, higher expression was observed in ovary and oviduct tissues during brooding. Lastly, higher m RNA expression of Yangzhou geese was detected during the ovulation period than that of Zhedong geese.Conclusions: Our findings reveal the sequence characterization and expression patterns of the CYP11A1 gene during the goose reproductive cycle, which may provides correlative evidence that CYP11A1 expression is important in reproduction activity. | Qi Xu Yadong Song Yang Chen Ran Liu Yang Zhang Yang Li Zhengyang Huang Wenming Zhao Guobin Chang Guohong Chen | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 2 |
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