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| 1 | Progress and records in the study of endogenetic mineralization during collisional orogenesis显示文摘To develop and perfect the theory of plate tectonics and regional metallogeny, metal-logenesis during collisional orogenesis should be thoroughly studied and will attract increasing at-tention of more and more scientists. This paper presents the main aspects of research and discus-sions on metallogenesis during collisional orogenesis after the development of plate tectonics, and accordingly divides the study history into two stages, i.e. the junior stage during 1971-1990 and the senior stage after 1990. Beginning with the negation of mineralization in the collision regime by Guild (1971), the focus of study was put on whether there occurred any mineralization during collisional orogenesis at the junior stage. At the senior stage, which is initiated by the advance of met-allogenic and petrogenic model for collisional orogenesis, scientists begin to pay their attention to the geodynamic mechanism of metallogenesis, spatial and temporal distribution of ore deposits, ore-forming fluidization, relationship | Yanjing Chen Huayong Chen Yulin Liu Guangjun Guo Yong Lai Shan Qin Baoling Huang Zengjie Zheng Yinghui Sui Chao Li et al. | 2000 | Chinese Science Bulletin2000，45，1： | 13 |
| 2 | Low-Complexity Secure Network Coding Against Wiretapping Using Intra/Inter-generation Coding显示文摘Existing solutions against wiretapping attacks for network coding either bring significant bandwidth overhead or incur a high computational complexity.In order to reduce the security overhead of the existing solutions for securing network coding,a novel securing network coding paradigm is presented relying on two coding models:intra-generation coding and inter-generation coding.The basic idea to secure network coding using intra-generation coding is to limit the encryption operations for each generation,and then subject the scrambled and the remaining original source vectors to a linear transformation.This method is then generalized seamlessly using inter-generation coding by further exploiting the algebraic structure of network coding.We show that the proposed schemes have properties of low-complexity security,little bandwidth consumption,and high efficiency in integrating with the existing security techniques effectively. | LIU Guangjun LIU Binyue LIU Ximeng LI Fang GUO Wangmei | 2015 | China Communications2015，12，6： | 9 |
| 3 | Attenuation ofβ-Amyloid Toxicity In Vitro and In Vivo by Accelerated Aggregation显示文摘Accumulation and aggregation of β-amyloid(Aβ) peptides result in neuronal death, leading to cognitive dysfunction in Alzheimer's disease. The self-assembled Aβ molecules form various intermediate aggregates including oligomers that are more toxic to neurons than the mature aggregates, including fibrils. Thus, one strategy to alleviate Aβ toxicity is to facilitate the conversion of Aβ intermediates to larger aggregates such as fibrils. In this study, we designed a peptide named A3 that significantly enhanced the formation of amorphous aggregates of Aβ by accelerating the aggregation kinetics. Thioflavin T fluorescence experiments revealed an accelerated aggregation of Aβ monomers, accompanying reduced Aβ cytotoxicity. Transgenic Caenorhabditis elegans over-expressing amyloid precursor protein exhibited paralysis due to the accumulation of Aβ oligomers, and this phenotype was attenuated by feeding the animals with A3 peptide. These findings suggest that the Aβ aggregation-promotion effect can potentially be useful for developing strategies to reduce Aβ toxicity. | Aihua Yang Chenxuan Wang Baomin Song Wendi Zhang Yuanyuan Guo Rong Yang Guangjun Nie Yanlian Yang Chen Wang | 2017 | Neuroscience Bulletin2017，33，4： | 3 |
| 4 | Personalized cancer vaccines from bacteria-derived outer membrane vesicles with antibody-mediated persistent uptake by dendritic cells显示文摘Nanocarriers with intrinsic immune adjuvant properties can activate the innate immune system while delivering tumor antigen,thus efficiently facilitating antitumor adaptive immunity.Bacteria-derived outer membrane vesicles(OMVs)are an excellent candidate due to their abundance of pathogen associated molecular patterns.However,during the uptake of OMVs by dendritic cells(DCs),the interaction between lipopolysaccharide and toll-like receptor 4 induces rapid DC maturation and uptake blockage,a phenomenon we refer to as“maturation-induced uptake obstruction'(MUO).Herein we decorated OMV with the DC-targeting aDEC205 antibody(OMV-DEC),which endowed the nanovaccine with an uptake mechanism termed as 4 | Jie Liang Keman Cheng Yao Li Jiaqi Xu Yiwei Chen Nana Ma Qingqing Feng Fei Zhu Xiaotu Ma Tianjiao Zhang Yale Yue Guangna Liu Xinjing Guo Zhiqiang Chen Xinwei Wang Ruifang Zhao Ying Zhao Jian Shi Xiao Zhao Guangjun Nie | 2022 | Fundamental Research2022，2，1： | 2 |
| 5 | Establishment and Application of a SYBR Green I Real-time Quantitative PCR Assay for the Detection of LAT Gene of Pseudorabies Virus in Bama Miniature Pigs显示文摘[Objective] This study aimed to establish a rapid,sensitive and specific SYBR Green I real-time quantitative PCR assay for the detection of latent pseudorabies virus( PRV) infection. [Method]SYBR Green I real-time quantitative PCR conditions and system for early detection of latent pseudorabies virus infection were optimized and compared with conventional PCR to investigate the sensitivity and specificity. Subsequently,the established assay was applied to detect different clinical samples. [Result]The sensitivity of SYBR Green I real-time quantitative PCR assay( 52 copies / μl) was 1 000 times higher than that of conventional PCR( 5. 2 × 104 copies / μl) and the detection time was shortened by 1 /2. The established assay could be used to detect PRV but could not be used to detect PCV2,PPV,CSFV or PRRSV. Various tissues were collected from Bama miniature pigs with latent PRV infection under sterile conditions for real-time PCR detection. Results showed that viral copy number in the brain,nasal swab,inguinal lymph node,liver,lung and spleen was above 20,while PRV was not detected in the kidney and heart tissues. [Conclusion]The established SYBR Green I real-time quantitative PCR assay for PRV LAT detection was specific,sensitive and rapid,which could be used for pathogen monitoring,epidemiological investigation and quantitative study of PRV. | Sufang LU Guangjun GUO Ling MO Feng WEI Bingmei DONG Wentong ZHANG Zhiqiang SHEN | 2015 | Agricultural Biotechnology2015，4，3： | 2 |
| 6 | Expression of Pseudorabies Virus gE Core Epitopes in Escherichia coli Strain BL21 and Utilization of Indirect PRV gE-ELISA显示文摘Pseudorabies virus glycoprotein E( PRV gE) has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce gE antigen in large quantities and at low cost,a gene fragment encoding PRV gE core epitopes was expressed in E. coli BL21 expression system. SDS-PAGE and Western Blotting revealed that the expression product in culture supernatant of E. coli BL21 was a recombinant protein,approximately 51. 8 Kd. At 5 h post-induction,protein concentration assay showed that the expression product amounted to 1. 65 mg /ml,accounting for 24. 17% of total proteins in the culture supernatant. An indirect PRV gE-ELISA was established by using the recombinant expression product as a coating antigen. Cross-reactivity assay showed that this antigen was PRV specific. In addition,the assay was consistently reproducible. Comparison of detection results of 240 serum samples between PRV gE-ELISA and a commercially available PRV diagnostic kit showed that there was no significant difference between these two methods( P > 0. 05). | Guangjun GUO Sufang LU Guanggang QU Feng LI Lin DONG Yanli BI Jinliang WANG Feng WEI Na TANG Chunling ZHANG Zhuang DING Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
| 7 | Hydrogen-rich gas from catalytic steam gasification of municipal solid waste (MSW): influence of catalyst and temperature on yield and product composition 显示文摘 | He Maoyun Hu Zhiquan Xiao Bo Li Jianfen Guo Xianjun Luo Siyi Yang Fan Feng Yu Yang Guangjun Liu Shiming | 2009 | International Journal of Hydrogen Energy2009，34，1： | 1 |
| 8 | 16S rRNA Cloning and Identification of a Strain Isolated from Intestinal Tract of Rex Rabbit显示文摘This study was conducted to identify bacterial strain He Nan-001 isolated from intestinal tract of healthy rex rabbit.The strain was identified by gram staining,and OD_(600) values at different culture time were measured to develop a bacterial growth curve.The metabolic pathways of the bacterium using sugar in fermentation was identified with biochemical tubes.Total RNA of the strain was extracted,and total cDNA was obtained by Oligo(dT) method.Primers were designed using Primer 5.0 bio-software according to the 16S rRNA sequence published in Gen Bank,through cloning,ligation to vector PMD18T,construction of PMD18T/16S rRNA vector and transformation into DH5α,plasmid was extracted and sequenced,and the sequencing result was compared with sequences in NCBI followed by drawing of phylogenetic tree.The strain was verified to be double-stranded gram-positive cocci,which could ferment glucose,mannose,maltose and sorbitol,producing acids production,but failed to ferment sucrose,serum inulin and β-galactosidase.The phylogenetic tree analysis showed that the isolated bacterium shared the highest homology with an India isolate,and belongs to diplostreptococcus.This study provides significance experimental data and theoretical guidance for further development and utilization of the bacterial strain. | Guangjun GUO Yumao WANG Sufang LYU Jing HAN Xinming WU Hanjie ZHANG Qiqi ZOU Qiang FU Zhiqiang SHEN | 2016 | Agricultural Biotechnology2016，5，5： | 1 |
| 9 | 2-Level R-tree Index Based on Spatial Grids and Hilbert R-tree显示文摘Multi-level spatial index techniques are always used in large spatial databases. After a general survey of R-tree relevant techniques, this paper presents a novel 2-level index structure, which is based on the schemas of spatial grids, Hilbert R-tree and common R-tree. This structure is named H2R-tree, and it is specifically suitable for the indexing highly skewed, distributed, and large spatial database. Algorithms and a sample are given subsequently. | GUO Jing LIU Guangjun DONG Xurong GUO Lei | 2006 | Geo-Spatial Information Science2006，9，2： | 1 |
| 10 | Effects of sulfate-reducing bacteria on methylmercury at the sediment–water interface显示文摘Sediment cores(containing sediment and overlying water) from Baihua Reservoir(SW China)were cultured under different redox conditions with different microbial activities, to understand the effects of sulfate-reducing bacteria(SRB) on mercury(Hg) methylation at sediment–water interfaces. Concentrations of dissolved methyl mercury(DMe Hg) in the overlying water of the control cores with bioactivity maintained(BAC) and cores with only sulfate-reducing bacteria inhibited(SRBI) and bacteria fully inhibited(BACI) were measured at the anaerobic stage followed by the aerobic stage. For the BAC and SRBI cores, DMe Hg concentrations in waters were much higher at the anaerobic stage than those at the aerobic stage, and they were negatively correlated to the dissolved oxygen concentrations(r =- 0.5311 and r =- 0.4977 for BAC and SRBI, respectively). The water DMe Hg concentrations of the SRBI cores were 50% lower than those of the BAC cores, indicating that the SRB is of great importance in Hg methylation in sediment–water systems, but there should be other microbes such as iron-reducing bacteria and those containing specific gene cluster(hgc AB), besides SRB,causing Hg methylation in the sediment–water system. | Lingxia Zeng Guangjun Luo Tianrong He Yanna Guo Xiaoli Qian | 2016 | Journal of Environmental Sciences2016，28，8： | 1 |
| 11 | Characterization of Natural Killer Cells in the Liver of Young Mice显示文摘OBJECTIVE To determine the quantity and quality of liver NK cells from young and adult mice and compare their characteristics. METHODS C57BL/6 mice were used at 2 weeks (young) and 8 weeks (adult) of age. The percentage and absolute number of NK cells in the liver and spleen were analyzed. The cytotoxicity of NK cells in the liver and spleen against various targets were detected by a 4 h 51Cr-release method. FACScan was used to analyze the expression of CD69, Mac-1, Ly49C/I and CD94 on the NK cells. Perforin mRNA levels were analyzed by the reverse transcription polymerase chain reaction (RT-PCR). RESUTLS The percentages of NK cells in the liver of young and adult mice were similar (11.9%±1.7% vs. 9.9%±1.6%, P>0.05), but the absolute number per liver weight was higher in the young animals (11.6±2.5x105/g vs. 3.4±0.8x105/g, P<0.05). The level of NK cytotoxicity was extremely high in the liver of young compared to adult mice (71.0%±5.5% vs. 23.8%±4.4%, P<0.05), but this difference was not observed in the spleen. Phenotypes of the liver NK cells from young and adult mice were completely different from each other. The liver NK cells from young mice were CD69high Mac-1lowLy49C/ Ilow, whereas NK cells from older mice displayed inverse antigen levels (CD-69low Mac-1high Ly49C/Ihigh). The expression levels of other NK cell-related markers were similar in both groups.The perforin mRNA level in the liver lymphocytes from young mice was consistently greater compared to adult mice. CONCLUSION From 2 to 8 weeks C57BL/6 mice liver NK cells undergo age-associated changes. At 2 weeks of age the liver NK cells showed a high level of NK cytotoxicity and a unique phenotype which was not apparent at 8 weeks of age. | Peng Zhao Jiansheng Guo Guangjun Wang Qiang Chi Dequan Wu Zhaolin Zeng Xuefeng Bai Xishan Hao | 2007 | Chinese Journal of Clinical Oncology2007，4，1： | 1 |
| 12 | Performance and EXIT analysis of parallel concatenated space time trellis codes显示文摘Space time trellis coding (STTC) techniques have been proposed to achieve both diversity and coding gains in multiple input multiple output (MIMO) fading channels. But with more transmit antennas STTCs suffer from the design dificulty and complexity increasing. This paper proposes a scheme, named parallel concatenated space time trellis codes (PC-STTC), to achieve the tradeoff between the performances and complexity of STTCs for a large number of transmit antennas. Simulation results and complexity comparison are provided to demonstrate the performance and superiority of the proposed scheme over conventional schemes in fast fading channels in low signal-to-noise ratio (SNR) regions. And an EXIT (extrinsic information transform) chart is given to analyze the iterative convergence of the proposed scheme. It shows that PC-STTC has better iterative convergence in low SNR regions. | Yang Haifen Li Guangjun Guo Zhiyong Xiong Yong | 2009 | Journal of Systems Engineering and Electronics2009，20，6： | 0 |
| 13 | Hydrothermal synthesis of zeolitic material from circulating fluidized bed combustion fly ash for the highly efficient removal of lead from aqueous solution显示文摘The utilization of coal fly ash derived from circulating fluidized bed combustion(CFBFA)still faces great challenges because of its unique characteristics.In this study,a zeolitic material with Na-P1 zeolite as the main phase was successfully synthesized via a hydrothermal method by using CFBFA as the raw material.The effects of hydrothermal temperature,time,and added CTAB amount on the characterizations of synthesized materials were investigated by XRD,SEM,and XPS.The properties of the optimal zeolitic material and its adsorption performance for Pb^(2+)in aqueous solution were evaluated.The influences of pH,initial concentration,dosage,and temperature on Pb^(2+)adsorption were also examined.Results revealed the following optimal parameters for the synthesis of zeolitic material:NaOH concentration of 2 mol·L^(-1),solid-to-liquid ratio of 1:10 g·ml^(-1),hydrothermal temperature of 110℃,hydrothermal time of 9 h,and CTAB amount of 1 g(per 100 ml solution).The adsorption capacities of the zeolitic material reached 329.67,424.69,and 542.22 mg·g^(-1) when the pH values of aqueous solution were 5,6,and 7,respectively.The Pb^(2+)removal efficiency can reach more than 99%in aqueous solution with the initial concentrations of 100-300 mg·L^(-1) under pH 6 and suitable adsorbent dosage.The adsorption and kinetics of Pb^(2+)on the zeolitic material can be described by Langmuir isotherm and pseudo-second-order kinetic models,respectively.The ion exchange between Pb^(2+)and Na^(+)and chemisorption are the main adsorption mechanism.All these findings imply that the synthesis of low-cost adsorbent for Pb^(2+)removal from weak acid and neutral aqueous solution provides a highly effective method to utilize CFBFA. | Zhibin Ma Xueli Zhang Guangjun Lu Yanxia Guo Huiping Song Fangqin Cheng | 2022 | Chinese Journal of Chemical Engineering2022，35，7： | 0 |
| 14 | Blockchain-Based Cognitive Computing Model for Data Security on a Cloud Platform显示文摘Cloud storage is widely used by large companies to store vast amounts of data and files,offering flexibility,financial savings,and security.However,information shoplifting poses significant threats,potentially leading to poor performance and privacy breaches.Blockchain-based cognitive computing can help protect and maintain information security and privacy in cloud platforms,ensuring businesses can focus on business development.To ensure data security in cloud platforms,this research proposed a blockchain-based Hybridized Data Driven Cognitive Computing(HD2C)model.However,the proposed HD2C framework addresses breaches of the privacy information of mixed participants of the Internet of Things(IoT)in the cloud.HD2C is developed by combining Federated Learning(FL)with a Blockchain consensus algorithm to connect smart contracts with Proof of Authority.The“Data Island”problem can be solved by FL’s emphasis on privacy and lightning-fast processing,while Blockchain provides a decentralized incentive structure that is impervious to poisoning.FL with Blockchain allows quick consensus through smart member selection and verification.The HD2C paradigm significantly improves the computational processing efficiency of intelligent manufacturing.Extensive analysis results derived from IIoT datasets confirm HD2C superiority.When compared to other consensus algorithms,the Blockchain PoA’s foundational cost is significant.The accuracy and memory utilization evaluation results predict the total benefits of the system.In comparison to the values 0.004 and 0.04,the value of 0.4 achieves good accuracy.According to the experiment results,the number of transactions per second has minimal impact on memory requirements.The findings of this study resulted in the development of a brand-new IIoT framework based on blockchain technology. | Xiangmin Guo Guangjun Liang Jiayin Liu Xianyi Chen | 2023 | Computers, Materials & Continua2023，77，12： | 0 |
| 15 | The Behavior and Characteristics of the InterfacialWaves in Gas-Liquid Two-Phase Separated FlowThrough Downward Inclined Rectangular Channel显示文摘An experimental investigation on the behavior and characteristics of interfacial waves in downward inclined rectangular channel was conducted. The interfacial waves were traced and measured by us-ing conductance technique. The wav patterns were distinguished and defined. The characteristics of the interfacial waves, such as time-averaged film thickness, wave height, wave propagation speed,wavlength and wave frequency, were systematically examined in terms of gas and liquid superficial volumetric fiuxes. The effect of the inclination and flow channel geometry of the test section on the interfacial wav was also investigated. | Guangjun Li Lienjin Guo Xuejun Chen(National Laboratory of Multiphase Flow in Power Engineering,Xi’an Jiaotong University,Xi’an,710049,China) | 1998 | Journal of Thermal Science1998，7，1： | 0 |
| 16 | Research Progress in Prevention and Treatment of Bacterial Diseases by Traditional Chinese Medicine显示文摘Recently,antibiotics have been widely used to resist various bacterial diseases in livestock breeding,which generated more superbacteria with resistance. Food safety problems,such as animal drug residues are urgent to be solved. Traditional Chinese medicines are traditional treasures of China,which attract more and more attention in the prevention and treatment of bacterial diseases because of their abundant advantages including improving immunologic function of organisms,antibacterial,antivirus,antitumor,anti-inflammatory and improving animal performance. The advantages of traditional Chinese medicines,such as low toxicity,low residual,green and safe,conform to food safety requirements. The using condition of traditional Chinese medicines in main bacterial diseases of animals was reviewed in the paper,thus to provide a reference for livestock production. | Sufang LYU Guangjun GUO Ling MO Feng LI Feng WEI Bingmei DONG Zhiqiang SHEN | 2016 | Agricultural Biotechnology2016，5，4： | 0 |
| 17 | Detection of Serum Antibodies of Porcine Pseudorabies Virus(PRV)in Binzhou City in 2014显示文摘To understand immunization effect of pseudorabies vaccine and infection status of porcine pseudorabies( PR) in pig farms and guide prevention and control against PR,453 copies of blood samples collected from 43 different scale pig farms in four counties( districts) of Binzhou City were detected with ELISA to investigate PRV g B antibody and g E antibody. Detection results showed that the g B antibody positive rate of sows was 75. 58%,and that of fattening pigs was68. 67%; the pig farms with positive rate higher than 70% accounted for 74. 42% of total survey pig farms. The PRV g E antibody positive rates of sows and fattening pigs were 25. 41% and 26. 67%,and the positive rates of pig farms were 46. 51% and 44. 33%,respectively. There were regional differences among counties( districts). | Li Feng Lv Sufang Zhang Wentong Liu Jishan Li Shuguang Guo Guangjun Zhang Songlin Wang Wenxiu | 2017 | Animal Husbandry and Feed Science2017，9，1： | 0 |
| 18 | Determination of the Free Surface in a Liquid Tank显示文摘This article offere an easy optha method to determine the free surfare ina liquld tank. Computational examples show that it is effective and can be used for manysimilar problems. | Dahai GUO Guangjun HE Yaosong CHEN(Dept. of Mech., Peking Univ., Beijing 100871)E-mail: guodh@pub .mech.pku. edu. cn | 1996 | Communications in Nonlinear Science and Numerical Simulation1996，1，3： | 0 |
| 19 | Research on Metaverse Security and Forensics显示文摘As a subversive concept,the metaverse has recently attracted widespread attention around the world and has set off a wave of enthusiasm in academic,industrial,and investment circles.However,while the metaverse brings unprecedented opportunities for transformation to human society,it also contains related risks.Metaverse is a digital living space with information infrastructure,interoperability system,content production system,and value settlement system as the underlying structure in which the inner core is to connect real residents through applications and identities.Through social incentives and governance rules,the metaverse reflects the digital migration of human society.This article will conduct an in-depth analysis of the metaverse from the perspective of electronic data forensics.First,from the perspective of Internet development,the background and development process of the metaverse is discussed.By systematically elaborating on the concept and connotation of the metaverse,this paper summarizes the different views of current practitioners,experts,and scholars on the metaverse.Secondly,from the perspective of metaverse security,the social risk and crime risks of the metaverse are discussed.Then the importance of metaverse forensics is raised.Third,from the perspective of blockchain,smart wearable devices,and virtual reality devices,the objects and characteristics of metaverse forensics have been studied in depth.Taking smart wearable devices as an example,this paper gives the relevant experimental process of smart bracelet forensics.Finally,many challenges faced by metaverse forensics are summarized by us which provide readers with some exploratory guidance. | Guangjun Liang Jianfang Xin Qun Wang Xueli Ni Xiangmin Guo Pu Chen | 2023 | Computers, Materials & Continua2023，77，10： | 0 |
| 20 | Expression of Porcine Circovirus Type 2 ORF2 Gene in Myocardial Cells of Detached Cherry Valley Duck显示文摘The objective of this study was to construct the recombinant eukaryotic expression plasmid of ORF2 gene harboring enhanced green fluorescent protein(EGFP) report gene. ORF2 gene of porcine circovirus type2 cloned by PCR was ligated to the expression vector pEGFP-N1,which contains enhanced green fluorescent protein(EGFP) report gene,the recombinant eukaryotic expression plasmid pEGFP-N1-ORF2 was constructed successfully and was transfected into prepared duck myocardial cells(DMCs) by lipofectin. According to the result,the fluorescence expression was directly detected with fluorescence microscope,and the expression of ORF2 were analyzed by RT-PCR and indirect immunofluorescence assay(IFA) respectively. About 48 h after transfection,green fluorescent can be observed on transfected cells: T-PCR and IFA were positive. This indicated that ORF2 gene of PCV2 was expressed efficiently in transfected duck myocardial cells. | Wenxiu WANG Sufang LU Shijun FU Guangjun GUO Ling MO Yueqiang XIAO Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，5： | 0 |
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