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| 1 | Study on the Fire Behavior of Sandwich Wall Panels with GFRP Skins and a Wood-Web Core显示文摘To investigate the temperature field and residual bearing capacity of the sandwich wall panels with GFRP skins and a wood-web core under a fire,three sandwich walls were tested.One of them was used for static load test and the other two for the one-side fire tests.Besides,temperature probe points were set on the sandwich walls to obtain the temperature distribution.Meanwhile,the model of the sandwich wall was established in the finite element software by the method of core material stiffness equivalent.The temperature distribution and performance reduction of materials were also considered.The residual bearing capacity of specimens after fire exposure were simulated considering the effects of web spacing,wall panel thickness and fire exposure time.Because the sandwich wall panels were stressed by eccentric compression after a fire,the residual compressive strength of the wall panel after the fire can be calculated through the eccentric loading analysis.Compared with the numerical results,it can be concluded that the effectiveness of calculation method of residual bearing capacity after fire exposure was proved. | Guangjun Sun Chuting Wang Lu Wang | 2022 | Journal of Renewable Materials2022，10，6： | 2 |
| 2 | Establishment and Application of a SYBR Green I Real-time Quantitative PCR Assay for the Detection of LAT Gene of Pseudorabies Virus in Bama Miniature Pigs显示文摘[Objective] This study aimed to establish a rapid,sensitive and specific SYBR Green I real-time quantitative PCR assay for the detection of latent pseudorabies virus( PRV) infection. [Method]SYBR Green I real-time quantitative PCR conditions and system for early detection of latent pseudorabies virus infection were optimized and compared with conventional PCR to investigate the sensitivity and specificity. Subsequently,the established assay was applied to detect different clinical samples. [Result]The sensitivity of SYBR Green I real-time quantitative PCR assay( 52 copies / μl) was 1 000 times higher than that of conventional PCR( 5. 2 × 104 copies / μl) and the detection time was shortened by 1 /2. The established assay could be used to detect PRV but could not be used to detect PCV2,PPV,CSFV or PRRSV. Various tissues were collected from Bama miniature pigs with latent PRV infection under sterile conditions for real-time PCR detection. Results showed that viral copy number in the brain,nasal swab,inguinal lymph node,liver,lung and spleen was above 20,while PRV was not detected in the kidney and heart tissues. [Conclusion]The established SYBR Green I real-time quantitative PCR assay for PRV LAT detection was specific,sensitive and rapid,which could be used for pathogen monitoring,epidemiological investigation and quantitative study of PRV. | Sufang LU Guangjun GUO Ling MO Feng WEI Bingmei DONG Wentong ZHANG Zhiqiang SHEN | 2015 | Agricultural Biotechnology2015，4，3： | 2 |
| 3 | Expression of Pseudorabies Virus gE Core Epitopes in Escherichia coli Strain BL21 and Utilization of Indirect PRV gE-ELISA显示文摘Pseudorabies virus glycoprotein E( PRV gE) has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce gE antigen in large quantities and at low cost,a gene fragment encoding PRV gE core epitopes was expressed in E. coli BL21 expression system. SDS-PAGE and Western Blotting revealed that the expression product in culture supernatant of E. coli BL21 was a recombinant protein,approximately 51. 8 Kd. At 5 h post-induction,protein concentration assay showed that the expression product amounted to 1. 65 mg /ml,accounting for 24. 17% of total proteins in the culture supernatant. An indirect PRV gE-ELISA was established by using the recombinant expression product as a coating antigen. Cross-reactivity assay showed that this antigen was PRV specific. In addition,the assay was consistently reproducible. Comparison of detection results of 240 serum samples between PRV gE-ELISA and a commercially available PRV diagnostic kit showed that there was no significant difference between these two methods( P > 0. 05). | Guangjun GUO Sufang LU Guanggang QU Feng LI Lin DONG Yanli BI Jinliang WANG Feng WEI Na TANG Chunling ZHANG Zhuang DING Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
| 4 | Mitochondrial temperature-responsive drug delivery reverses drug resistance in lung cancer显示文摘Reversal of cancer drug resistance remains a critical challenge in chemotherapy.Mitochondria-targeted drug delivery has been suggested to mitigate drug resistance in cancer.To overcome the intrinsic limitations in conventional mitochondrial targeting strategies,we develop mitochondrial temperature-responsive drug delivery to reverse doxorubicin(DOX)resistance in lung cancer.Results demonstrate that the thermoresponsive nanocarrier can prevent DOX efflux and facilitate DOX accumulation and mitochondrial targeting in DOX-resistant tumors.As a consequence,thermoresponsive nanocarrier enhances the cytotoxicity of DOX and reverses the drug resistance in tumor-bearing mice.This work represents the first example of mitochondrial temperature-responsive drug delivery for reversing cancer drug resistance. | Lifo Ruan Jun Chen Chuanchao Du Huiru Lu Jiayu Zhang Xiaomeng Cai Rui Dou Wenchu Lin Zhifang Chai Guangjun Nie Yi Hu | 2022 | Bioactive Materials2022，7，7： | 1 |
| 5 | Nanorods of La:Sn207 synthesized in ethanol solvent 显示文摘 | Wang Shumei Zhou Guangjun Lu Mengkai etal | 2006 | Journal of Alloys and Compounds2006，424，12： | 1 |
| 6 | Structure-activity relationship analysis of antioxidant ability and neuroprotective effect of gallic acid derivatives显示文摘 | Zhongbing Lu Guangjun Nie Peter S Belton | 2006 | Neurochem Int2006，48，4： | 1 |
| 7 | Luminescence properties of Sr5 ( PO4 ) 3 Cl : Ni^2 + nanocrystals 显示文摘 | Xiu Zhiliang Lu Mengkai Liu Suwen Zhang Haiping Zhou Guangjun | 2006 | Materials Letters2006，60，： | 1 |
| 8 | Structure-activity relationship analysis of antioxidant ability and neuroprotective effect of gallic acid derivatives显示文摘 | LU Zhongbing NIE Guangjun BELTON P S | 2006 | Neurochem Int2006，48，4： | 1 |
| 9 | Structure-activity relationship analysis of antioxidant ability and neuroprotective effect of gallic acid derivatives 显示文摘 | Lu Zhongbing Nie Guangjun Peter S Behon etal | 2006 | Neurochem Int2006，48，4： | 1 |
| 10 | Synthesis, characterization and luminescence of Eu3+-doped SrZrO3 nanocrystals 显示文摘 | Zhang Aiyu Lu Mengkai Zhou Guangjun | 2009 | J Alloys Comp2009，468，1： | 1 |
| 11 | Technologies for Asphalt Pavement Surface Testing in Road and Bridge Construction显示文摘Asphalt pavement is currently one of the main components in the construction of roads and bridges.However,from a practical point of view,various quality problems are prone to occur in the surface layer of asphalt pavement,which will lead to the poor overall quality of road and bridge projects.Therefore,it should be applied reasonably.Advanced testing technologies are used to test the mixture quality,compaction,segregation,thickness,and other aspects of the asphalt pavement surface layer,so as to improve the quality of the asphalt pavement surface layer,and then improve the overall quality of road and bridge construction.Therefore,this paper mainly analyzes the technologies for asphalt pavement surface layer testing in road and bridge engineering construction. | Lu Zhang Guangjun Li Lin Nan Shuyan Liu | 2023 | Journal of Architectural Research and Development2023，7，2： | 0 |
| 12 | Hydrothermal synthesis of zeolitic material from circulating fluidized bed combustion fly ash for the highly efficient removal of lead from aqueous solution显示文摘The utilization of coal fly ash derived from circulating fluidized bed combustion(CFBFA)still faces great challenges because of its unique characteristics.In this study,a zeolitic material with Na-P1 zeolite as the main phase was successfully synthesized via a hydrothermal method by using CFBFA as the raw material.The effects of hydrothermal temperature,time,and added CTAB amount on the characterizations of synthesized materials were investigated by XRD,SEM,and XPS.The properties of the optimal zeolitic material and its adsorption performance for Pb^(2+)in aqueous solution were evaluated.The influences of pH,initial concentration,dosage,and temperature on Pb^(2+)adsorption were also examined.Results revealed the following optimal parameters for the synthesis of zeolitic material:NaOH concentration of 2 mol·L^(-1),solid-to-liquid ratio of 1:10 g·ml^(-1),hydrothermal temperature of 110℃,hydrothermal time of 9 h,and CTAB amount of 1 g(per 100 ml solution).The adsorption capacities of the zeolitic material reached 329.67,424.69,and 542.22 mg·g^(-1) when the pH values of aqueous solution were 5,6,and 7,respectively.The Pb^(2+)removal efficiency can reach more than 99%in aqueous solution with the initial concentrations of 100-300 mg·L^(-1) under pH 6 and suitable adsorbent dosage.The adsorption and kinetics of Pb^(2+)on the zeolitic material can be described by Langmuir isotherm and pseudo-second-order kinetic models,respectively.The ion exchange between Pb^(2+)and Na^(+)and chemisorption are the main adsorption mechanism.All these findings imply that the synthesis of low-cost adsorbent for Pb^(2+)removal from weak acid and neutral aqueous solution provides a highly effective method to utilize CFBFA. | Zhibin Ma Xueli Zhang Guangjun Lu Yanxia Guo Huiping Song Fangqin Cheng | 2022 | Chinese Journal of Chemical Engineering2022，35，7： | 0 |
| 13 | Analysis of New Materials Application in Municipal Road Construction显示文摘In order to promote the development of road traffic in our country,it is necessary to strengthen the research on municipal road construction technology,constantly innovate construction technology and construction technique,and then effectively ensure the rapid development of urban traffic.This paper mainly elaborates on asphalt road surface regeneration technology,modified asphalt concrete,concrete road surface anti-cracking technology,three-dimensional printing technology,composite material road surface,polymer composite materials,etc.,to ensure the development of urban transportation and the quality of municipal road projects. | Guangjun Li Lu Zhang Lin Nan Manman Feng Shuyan Liu | 2023 | Journal of Architectural Research and Development2023，7，3： | 0 |
| 14 | Research on Infrared Image Fusion Technology Based on Road Crack Detection显示文摘This study aimed to propose road crack detection method based on infrared image fusion technology.By analyzing the characteristics of road crack images,this method uses a variety of infrared image fusion methods to process different types of images.The use of this method allows the detection of road cracks,which not only reduces the professional requirements for inspectors,but also improves the accuracy of road crack detection.Based on infrared image processing technology,on the basis of in-depth analysis of infrared image features,a road crack detection method is proposed,which can accurately identify the road crack location,direction,length,and other characteristic information.Experiments showed that this method has a good effect,and can meet the requirement of road crack detection. | Guangjun Li Lin Nan Lu Zhang Manman Feng Yan Liu Xu Meng | 2023 | Journal of World Architecture2023，7，3： | 0 |
| 15 | A conserved unusual posttranscriptional processing mediated by short,direct repeated (SDR) sequences in plants显示文摘In several stress responsive gene loci of monocot cereal crops,we have previously identified an unusual posttranscriptional processing mediated by paired presence of short direct repeated (SDR) sequences at 5' and 3' splicing junctions that are distinct from conventional (U2/U12-type) splicing boundaries.By using the known SDR-containing sequences as probes,24 plant candidate genes involved in diverse functional pathways from both monocots and dicots that potentially possess SDR-mediated posttranscriptional processing were predicted in the GenBank database.The SDRs-mediated posttranscriptional processing events including cis-and trans-actions were experimentally detected in majority of the predicted candidates.Extensive sequence analysis demonstrates several types of SDR-associated splicing peculiarities including partial exon deletion,exon fragment repetition,exon fragment scrambling and trans-splicing that result in either loss of partial exon or unusual exonic sequence rearrangements within or between RNA molecules.In addition,we show that the paired presence of SDR is necessary but not sufficient in SDR-mediated splicing in transient expression and stable transformation systems.We also show prokaryote is incapable of SDR-mediated premRNA splicing. | Xiangli Niu Di Luo Shaopei Gao Guangjun Ren Lijuan Chang Yuke Zhou Xiaoli Luo Yuxiang Li Pei Hou Wei Tang Bao-Rong Lu Yongsheng Liu | 2010 | Journal of Genetics and Genomics2010，37，1： | 0 |
| 16 | Possible Octupole Correlation in ^(90)Mo显示文摘The nuclei with octupole deformation have a feature of reflection asymmetry and so there exists a wealth of information about nuclear property. Therefore, study on behavior of high spin states for these nuclei is helpful to know nuclear structure further. Theories predict that octupole deformation with β3≠0 will occurs when the proton number Z and neutron number N are 56, 88, and 132. | LI Guang-sheng WU Xiao-guang PENG Zhao-hua WEN Shu-xian HAN Guang-bing LI Cheng-bo LU Shao-jun WU Shao-yong YUAN GuangJun YANG Chun-xiang ZHU Li-hua | 2003 | Annual Report of China Institute of Atomic Energy2003，，1： | 0 |
| 17 | Expression of Porcine Circovirus Type 2 ORF2 Gene in Myocardial Cells of Detached Cherry Valley Duck显示文摘The objective of this study was to construct the recombinant eukaryotic expression plasmid of ORF2 gene harboring enhanced green fluorescent protein(EGFP) report gene. ORF2 gene of porcine circovirus type2 cloned by PCR was ligated to the expression vector pEGFP-N1,which contains enhanced green fluorescent protein(EGFP) report gene,the recombinant eukaryotic expression plasmid pEGFP-N1-ORF2 was constructed successfully and was transfected into prepared duck myocardial cells(DMCs) by lipofectin. According to the result,the fluorescence expression was directly detected with fluorescence microscope,and the expression of ORF2 were analyzed by RT-PCR and indirect immunofluorescence assay(IFA) respectively. About 48 h after transfection,green fluorescent can be observed on transfected cells: T-PCR and IFA were positive. This indicated that ORF2 gene of PCV2 was expressed efficiently in transfected duck myocardial cells. | Wenxiu WANG Sufang LU Shijun FU Guangjun GUO Ling MO Yueqiang XIAO Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，5： | 0 |
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