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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Effects of different resuscitation fluid on severe acute pancreatitis显示文摘AIM: To compare effects of different resuscitation fluid on microcirculation, inflammation, intestinal barrier and clinical results in severe acute pancreatitis (SAP). METHODS: One hundred and twenty patients with SAP were enrolled at the Pancreatic Disease Institute between January 2007 and March 2010. The patients were randomly treated with normal saline (NS group), combination of normal saline and hydroxyethyl starch (HES) (SH group), combination of normal saline, hydroxyethyl starch and glutamine (SHG group) in resuscitation. The ratio of normal saline to HES in the SH and SHG groups was 3:1. The glutamine (20% glutamine dipeptide, 100 mL/d) was supplemented into the resuscitation liquid in the SHG group. Complications and outcomes including respiratory and abdominal infection, sepsis, abdominal hemorrhage, intra-abdominal hypertension, abdominal compartment syndrome (ACS), renal failure, acute respiratory distress syndrome (ARDS), multiple organ dysfunction syndrome (MODS), operation intervention, length of intensive care unit stay, length of hospital stay, and mortality at 60 d were compared. Moreover, blood oxygen saturation (SpO 2 ), gastric intramucosal pH value (pHi), intra-abdominal pressure (IAP), inflammation cytokines, urine lactulose/mannitol (L/M) ratio, and serum endotoxin were investigated to evaluate the inflammatory reaction and gut barrier. RESULTS: Compared to the NS group, patients in the SH and SHG groups accessed the endpoint more quickly (3.9 ± 0.23 d and 4.1 ± 0.21 d vs 5.8 ± 0.25 d, P < 0.05) with less fluid volume (67.26 ± 28.53 mL/kg/d, 61.79 ± 27.61 mL/kg per day vs 85.23 ± 21.27 mL/kg per day, P < 0.05). Compared to the NS group, incidence of renal dysfunction, ARDS, MODS and ACS in the SH and SHG groups was obviously lower. Furthermore, incidence of respiratory and abdominal infection was significantly decreased in the SH and SHG groups, while no significant difference in sepsis was seen. Moreover, less operation time was needed in the SH and SHG group than the NS group, but the difference was not significant. The mortality did not differ significantly among these groups. Blood SpO 2 and gastric mucosal pHi in the SH and SHG groups increased more quickly than in the NS group, while IAP was significantly decreased in the SH and SHG group. Moreover, the serum tumor necrosis factor-α, interleukin-8 and C-reactive protein levels in the SH and SHG groups were obviously lower than in the NS group at each time point. Furthermore, urine L/M ratio and serum endotoxin were significantly lower in the SH group and further decreased in the SHG group.CONCLUSION: Results indicated that combination of normal saline, HES and glutamine are more efficient in resuscitation of SAP by relieving inflammation and sustaining the intestinal barrier. | Gang Zhao Jun-Gang Zhang He-Shui Wu Jin Tao Qi Qin Shi-Chang Deng Yang Liu Lin Liu Bo Wang Kui Tian Xiang Li Shuai Zhu Chun-You Wang | 2013 | World Journal of Gastroenterology2013，19，13： | 56 |
| 3 | Influences of blood lipids on the occurrence and prognosis of hemorrhagic transformation after acute cerebral infarction: a case-control study of 732 patients显示文摘Background: To study the influence of blood lipid levels on hemorrhagic transformation(HT) and prognosis after acute cerebral infarction(ACI).Methods: Patients with ACI within 72 h of symptoms onset between January 1 st, 2015, and December 31 st, 2016, were retrospectively analyzed. Patients were divided into group A(without HT) and group B(HT). The outcomes were assessed after 3 months of disease onset using the modified Rankin Scale(m RS). An m RS score of 0–2 points indicated excellent prognosis, and an m RS score of 3–6 points indicated poor prognosis.Results: A total of 732 patients conformed to the inclusion criteria, including 628 in group A and 104 in group B. The incidence of HT was 14.2%, and the median onset time was 2 d(interquartile range, 1–7 d). The percentages of patients with large infarct size and cortex involvement in group B were 80.8% and 79.8%, respectively, which were both significantly higher than those in group A(28.7 and 33.4%, respectively). The incidence rate of atrial fibrillation(AF) in group B was significantly higher than that in group A(39.4% vs. 13.9%, P<0.001). The adjusted multivariate analysis results showed that large infarct size, cortex involvement and AF were independent risk factors of HT, while total cholesterol(TC) was a protective factor of HT(OR=0.359, 95% CI 0.136–0.944, P=0.038). With every 1 mmol/L reduction in normal TC levels, the risk of HT increased by 64.1%. The mortality and morbidity at 3 months in group B(21.2% and 76.7%, respectively) were both significantly higher than those in group A(8.0% and 42.8%, respectively). The adjusted multivariate analysis results showed that large infarct size(OR=12.178, 95% CI 5.390–27.516, P<0.001) was an independent risk factor of long-term unfavorable outcomes, whereas low-density lipoprotein cholesterol(LDL-C) was a protective factor(OR=0.538, 95% CI 0.300–0.964, P=0.037). With every 1 mmol/L reduction in normal LDL-C levels, the risk of an unfavorable outcome increased by 46.2%. Major therapies, including intravenous recombinant human tissue plasminogen activator(r TPA), intensive lipid-lowering statins and anti-platelets, were not significantly related to either HT or long-term, post-ACI poor prognosis.Conclusions: For patients with large infarct sizes, especially those with cortex involvement, AF, or lower levels of TC, the risk of HT might increase after ACI. The risk of a long-term unfavorable outcome in these patients might increase with a reduction in LDL-C. | Gang Lv Guo-Qiang Wang Zhen-Xi Xia Hai-Xia Wang Nan Liu Wei Wei Yong-Hua Huang Wei-Wei Zhang | 2019 | Military Medical Research2019，6，3： | 60 |
| 4 | SHRIMP zircon U-Pb dating for subduction-related gran-itic rocks in the northern part of east Junggar, Xinjiang显示文摘SHRIMP U-Pb zircon dating on the Xileketehalasu granodiorite porphyry and Kalasayi monodiorite porphyry that intrude middle Devonian Beitashan Formation at the north part of east Junggar region shows that they were formed at 381±6 Ma and 376±10 Ma respectively. They are interpreted as subduction-related granitic rocks, which is the first report that the isotopic ages for the granitic rocks range from 350 to 390 Ma. Another determined age for the Kalasayi monodiorite porphyry is 408±9 Ma, representing the age of underlain Lower Devonian volcanic rocks. Thus, the U-Pb dates suggest that the northeastward subduction of Junggar ocean from southwest occurred at 408 to 376 Ma (the real inter- val may be larger). Because the ore-bearing porphyry intruded following the formation of the volcanic rocks of middle Devonian Beitashan Formation, their tec- tonic setting is similar to the Andes Mountains that hosts world-class porphyry copper deposits, and the researched area could be regarded as a potential area for prospecting large porphyry copper deposits. | ZHANG Zhaochong YAN Shenghao CHEN Bailin ZHOU Gang HE Yongkang CHAI Fengmei HE Lixin WAN Yusheng | 2006 | Chinese Science Bulletin2006，51，8： | 43 |
| 5 | Postconditioning of sevoflurane and propofol is associated with mitochondrial permeability transition pore显示文摘Background: Sevoflurane and propofol are effective cardioprotective anaesthetic agents, though the cardioprotection of propofol has not been shown in humans. Their roles and underlying mechanisms in anesthetic postconditioning are unclear. Mitochondrial permeability transition pore (MPTP) opening is a major cause of ischemia-reperfusion injury. Here we investigated sevoflurane- and propofol-induced postconditioning and their relationship with MPTP. Methods: Isolated perfused rat hearts were exposed to 40 min of ischemia followed by 1 h of reperfusion. During the first 15 min of reperfusion, hearts were treated with either control buffer (CTRL group) or buffer containing 20 μmol/L atractyloside (ATR group), 3% (v/v) sevoflurane (SPC group), 50 μmol/L propofol (PPC group), or the combination of atractyloside with respective anesthetics (SPC+ATR and PPC+ATR groups). Infarct size was determined by dividing the total necrotic area of the left ventricle by the total left ventricular slice area (percent necrotic area). Results: Hearts treated with sevoflurane or propofol showed significantly better recovery of coronary flow, end-diastolic pressures, left ventricular developed pressure and derivatives compared with controls. Sevoflurane resulted in more protective alteration of hemodynamics at most time point of reperfusion than propofol. These improvements were paralleled with the reduction of lactate dehydrogenase release and the decrease of infarct size (SPC vs CTRL: (17.48±2.70)% vs (48.47±6.03)%, P<0.05; PPC vs CTRL: (35.60±2.10)% vs (48.47±6.03)%, P<0.05). SPC group had less infarct size than PPC group (SPC vs PPC: (17.48±2.70)% vs (35.60±2.10)%, P<0.05). Atractyloside coadministration attenuated or completely blocked the cardioprotective effect of postconditioning of sevoflurane and propofol. Conclusion: Postconditioning of sevoflurane and propofol has cardio-protective effect against ischemia-reperfusion injury of heart, which is associated with inhibition of MPTP opening. Compared to propofol, sevoflurane provides superior protection of functional recovery and infarct size. | Wei HE Feng-jiang ZHANG Shao-ping WANG Gang CHEN Cong-cong CHEN Min YAN | 2008 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2008，9，2： | 48 |
| 6 | Prediction of urban human mobility using large-scale taxi traces and its applications显示文摘这篇论文在一个城市的出租车交通系统调查人的活动性模式。这个工作集中于预言 humanmobility fromdiscovering 模式在从城市的热点的旅客提取数量(PUQ ) 的数字。这份报纸建议改进 ARIMA 基于预言方法在一个热点预报旅客的空间时间的变化。有 4 000 出租车 GPS 的一个大规模 realworld 数据集合的评估跟踪超过一年表演一个预言错误仅仅 5.8% 。我们也探索预言途径的申请帮助司机发现他们的下位旅客。用历史的真实世界的数据的模拟结果证明与我们的指导,驱动程序能减少花的时间,距离旅行,到发现他们的下位旅客在 37.1% 和 6.4% ,分别地。 | Xiaolong LI Gang PAN Zhaohui WU Guande QI Shijian LI Daqing ZHANG Wangsheng ZHANG Zonghui WANG | 2012 | Frontiers of Computer Science2012，6，1： | 45 |
| 7 | Disposable circumcision suture device: clinical effect and patient satisfaction显示文摘根据我们的经验,经历割礼的病人主要关于疼痛和阴茎外观被担心。我们进行了未来的使随机化的试用估计一台新可处理的割礼缝术设备(DCSD ) 的好处。942 个病人的一个总数同等地被划分成三个组(常规割礼,商戒指和可处理的缝术设备组) 。在 DCSD 组的病人是有化合物 5% lidocaine 乳脂的 anesthetized,其它与 2% lidocaine 阴茎块。操作时间, intra 起作用的血损失,切口愈合时间, intra 起作用、手术后的疼痛,阴茎外观和全面满足度被测量。操作时间和 intra 起作用的血损失与传统的组相比在商戒指和缝术设备组是显著地更低的(P <0.001 ) 。Intra 起作用的疼痛与另外的二个组相比是在缝术设备组的更少(P <0.001 ) ;而手术后的疼痛在与另外的二个组相比的传统的组是更高的(P <0.001 ) 。设备(80.57%) 和商戒指(73.57%) 组织的在缝术的病人是与常规割礼组相比对阴茎外观满意的更多(20.06% , P <0.05 ) 。在缝术设备组的病人也比传统的组快显著地愈合了(P <0.01 ) 。全面满足率在缝术设备组(78.66%) 更好与相比常规(47.13%) 并且商戒指(50.00%) 组(P <0.05 ) 。DCSD 和 lidocaine 乳脂的联合导致了更短的操作和切口愈合时间,减少了 intra 起作用、手术后的疼痛并且改进了对化妆外观的耐心的满足。 | Bo-Dong Lv Shi-Geng Zhang Xuan-Wen Zhu Jie Zhang Gang Chen Min-Fu Chen Hong-Liang Shen Zai-Jun Pei Zhao-Dian Chen | 2014 | Asian Journal of Andrology2014，16，3： | 49 |
| 8 | The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis显示文摘Artemisia annua,通常已知的同样香甜的苦恼或 Qinghao,是到中国的一个灌木土著人并且长被用于药用的目的。A。annua 现在作为有势力抗疟药混合物的唯一的生来的来源全球性被栽培, artemisinin。这里,我们报导 A 的 1.74-gigabase 染色体的一个高质量的草稿集会。annua,高度异质接合,富于重复定序,并且包含 63 ? 226 编码蛋白质的基因,在定序的植物种类之中的最大的数字之一。我们发现了那,作为在 Asteraceae 的一些定序的染色体之一, A。annua 染色体包含对这大被子植物 clade 特定的很多基因。尤其是,扩大和编码涉及萜烯生合成的酶的基因的功能的多样化与 artemisinin biosynthetic 小径的进化一致。我们进一步由介绍那 A 的 transcriptome 揭示了。annua 发展了复杂 transcriptional 规章的网络位于 \O 下面 artemisinin 生合成。把转基因的 A 基于我们产生了的全面 genomic 和 transcriptomic 分析。artemisinin 高级的生产的 annua 线,它现在为大规模生产准备好了并且将从而帮助遇见增加 artemisinin 的全球需求的挑战。 | Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang | 2018 | Molecular Plant2018，11，6： | 47 |
| 9 | Sevoflurane postconditioning reduces myocardial reperfusion injury in rat isolated hearts via activation of PI3K/Akt signaling and modulation of Bcl-2 family proteins显示文摘Sevoflurane postconditioning reduces myocardial infarct size.The objective of this study was to examine the role of the phosphatidylinositol-3-kinase(PI3K)/Akt pathway in anesthetic postconditioning and to determine whether PI3K/Akt signaling modulates the expression of pro-and antiapoptotic proteins in sevoflurane postconditioning.Isolated and perfused rat hearts were prepared first,and then randomly assigned to the following groups:Sham-operation(Sham),ischemia/reperfusion(Con),sevoflurane postconditioning(SPC),Sham plus 100 nmol/L wortmannin(Sham+Wort),Con+Wort,SPC+Wort,and Con+dimethylsulphoxide(DMSO).Sevoflurane postconditioning was induced by administration of sevoflurane(2.5%,v/v) for 10 min from the onset of reperfusion.Left ventricular developed pressure(LVDP),left ventricular end-diastolic pressure(LVEDP),maximum increase in rate of LVDP(+dP/dt),maximum decrease in rate of LVDP(?dP/dt),heart rate(HR),and coronary flow(CF) were measured at baseline,R30 min(30 min of reperfusion),R60 min,R90 min,and R120 min.Creatine kinase(CK) and lactate dehydrogenase(LDH) were measured after 5 min and 10 min reperfusion.Infarct size was determined by triphenyltetrazolium chloride staining at the end of reperfusion.Total Akt and phosphorylated Akt(phospho-Akt),Bax,Bcl-2,Bad,and phospho-Bad were determined by Western blot analysis.Analysis of variance(ANOVA) and Student-Newman-Keuls' test were used to investigate the significance of differences between groups.The LVDP,±dP/dt,and CF were higher and LVEDP was lower in the SPC group than in the Con group at all points of reperfusion(P<0.05).The SPC group had significantly reduced CK and LDH release and decreased infarct size compared with the Con group [(22.9±8)% vs.(42.4±9.4)%,respectively;P<0.05].The SPC group also had increased the expression of phospho-Akt,Bcl-2,and phospho-Bad,and decreased the expression of Bax.Wortmannin abolished the cardioprotection of sevoflurane postconditioning.Sevoflurane postconditioning may protect the isolated rat heart.Activation of PI3K and modulation of the expression of pro-and antiapoptotic proteins may play an important role in sevoflurane-induced myocardial protection. | Li-na YU Jing YU Feng-jiang ZHANG Mei-juan YANG Ting-ting DING Jun-kuan WANG Wei HE Tao FANG Gang CHEN Min YAN | 2010 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2010，11，9： | 36 |
| 10 | Preventive effect of ulinastatin and gabexate mesylate on post-endoscopic retrograde cholangiopancreatography pancreatitis显示文摘背景 endoscopic 以后后退 cholangiopancreatography 胰腺炎(锐气) 被认为是之一使人烦恼的复杂并发症内视镜后退 cholangiopancreatography (ERCP ) 。在锐气上评估 ulinastatin 和 gabexate mesylate (美国通用汽车公司) 的预防效果的使随机化的控制试用的结果是矛盾的。现在的学习被设计与使随机化的控制试用(RCT ) 的元分析在锐气上评估 ulinastatin 和美国通用汽车公司的预防效果 .Methods 五个电子数据库被在找在锐气上评估 ulinastatin 和美国通用汽车公司的预防效果的 RCT。概括效果被估计,方法由 Cochrane Collaboration.Results 推荐了包含 5105 个参加者的 12 研究在我们的元分析被包括。ulinastatin 的管理减少了仅仅在足够的剂量的锐气的发生(或, 0.39;95% CI, 0.19 ~ 0.81;P=0.01 ) 。需要到对待(NNT ) 的数字是 6。并且 ulinastatin 的管理也减少了 post-ERCP hyperamylasemia (PEHA ) 的发生(或, 0.40;95% CI, 0.28 ~ 0.58;P < 0.000 01 ) 。高剂量的美国通用汽车公司的慢注入为锐气预防是有效的(或, 0.44; 95% CI, 0.25 ~ 0.79;P=0.006 ) ,并且低剂量的美国通用汽车公司的快速的注入也为锐气预防显示出功效(或, 0.37; 95% CI, 0.20 ~ 0.69;P=0.002 ) 。NNT 分别地是 7 和 6。然而,在低剂量并且由慢注入的美国通用汽车公司的管理是无效的(或, 0.99;95% CI, 0.64 ~ 1.55;P=0.98 ) 。美国通用汽车公司的管理有趋势减少 PEHA 率,然而并非到统计意义(或, 0.86;95% CI, 0.73 ~ 1.01;P=0.06 ) 。当低质量的研究被排除时,有二高质量的研究的元分析显示 ulinastatin 没减少锐气的率(或, 0.63;95% CI, 0.32 ~ 1.26;P=0.19 ) 并且 PEHA 发生(或, 0.80;95% CI, 0.31 ~ 2.07;P=0.64 ) 。有六高质量的研究的元分析证明美国通用汽车公司管理减少了锐气发生(或, 0.52;95% CI, 0.29 ~ 0.91; P=0.02 ) 不为 PEHA 预防是有效的(或, 0.88;95% CI, 0.74 ~ 1.04;P=0.12 ).Conclusions Ulinastatin 和美国通用汽车公司可能具有为锐气的预防的价值。美国通用汽车公司应该在高剂量并且由快速的注入被管理。并且 ulinastatin 的剂量应该是足够的。然而,结论不是 overwhelming.More 大样品的尺寸和高质量的 RCT 仍然被需要阐明二药的政府是否确实穿上预防效果锐气。 | ZHANG Zhi-feng YANG Ning ZHAO Gang ZHU Lei ZHU Ying WANG Li-xia | 2010 | Chinese Medical Journal2010，，18： | 38 |
| 11 | Effects of resource additions on species richness and ANPP in an alpine meadow community显示文摘Aims Theories based on resource additions indicate that plant species richness is mainly determined by the number of limiting resources.However,the individual effects of various limiting resources on species richness and aboveground net primary productivity(ANPP)are less well understood.Here,we analyzed potential linkages between additions of limiting resources,species loss and ANPP increase and further explored the underlying mechanisms.Methods Resources(N,P,K and water)were added in a completely randomized block design to alpine meadow plots in the Qinghai-Tibetan Plateau.Plant aboveground biomass,species composition,mean plant height and light availability were measured in each plot.Regression and analysis of variance were used to analyze the responses of these measures to the different resource-addition treatments.Important Findings Species richness decreased with increasing number of added limiting resources,suggesting that plant diversity was apparently determined by the number of limiting resources.Nitrogen was the most important limiting resource affecting species richness,whereas Pand K alone had negligible effects.The largest reduction in species richness occurred when all three elements were added in combination.Water played a different role compared with the other limiting resources.Species richness increased when water was added to the treatments with N and P or with N,P and K.The decreases in species richness after resource additions were paralleled by increases in ANPP and decreases in light penetration into the plant canopy,suggesting that increased light competitionwas responsible for the negative effects of resource additions on plant species richness. | Zhengwei Ren Qi Li Chengjin Chu Luqiang Zhao Jieqi Zhang Dexiecuo Ai Yingbo Yang Gang Wang | 2010 | Journal of Plant Ecology2010，3，1： | 40 |
| 12 | Prediction of risk factors for lymph node metastasis in early gastric cancer显示文摘AIM:To explore risk factors for lymph node metastases in early gastric cancer(EGC) and to confirm the appropriate range of lymph node dissection.METHODS:A total of 202 patients with EGC who underwent curative gastrectomy with lymphadenectomy in the Department of Surgery,Xinhua Hospital and Ruijin Hospital of Shanghai Jiaotong University Medical School between November 2003 and July 2009,were retrospectively reviewed.Both the surgical procedure and the extent of lymph node dissection were based on the recommendations of the Japanese gastric cancer treatment guidelines.The macroscopic type was classified as elevated(type Ⅰ or Ⅱa),flat(Ⅱb),or depressed(Ⅱc or Ⅲ).Histopathologically,papillary and tubular adenocarcinomas were grouped together as differentiated adenocarcinomas,and poorly differentiated and signet-ring cell adenocarcinomas were regarded as undifferentiated adenocarcinomas.Univariate and multivariate analyses of lymph node metastases and patient and tumor characteristics were undertaken.RESULTS:The lymph node metastases rate in patients with EGC was 14.4%.Among these,the rate for mucosal cancer was 5.4%,and 8.9% for submucosal cancer.Univariate analysis showed an obvious correlation between lymph node metastases and tumor location,depth of invasion,morphological classification and venous invasion(χ 2 = 122.901,P = 0.001;χ 2 = 7.14,P = 0.008;χ 2 = 79.523,P = 0.001;χ 2 = 8.687,P = 0.003,respectively).In patients with submucosal cancers,the lymph node metastases rate in patients with venous invasion(60%,3/5) was higher than in those without invasion(20%,15/75)(χ 2 = 4.301,P = 0.038).Multivariate logistic regression analysis revealed that the depth of invasion was the only independent risk factor for lymph node metastases in EGC [P = 0.018,Exp(B) = 2.744].Among the patients with lymph node metastases,29 cases(14.4%) were at N1,seven cases were at N2(3.5%),and two cases were at N3(1.0%).Univariate analysis of variance revealed a close relationship between the depth of invasion and lymph node metastases at pN 1(P = 0.008).CONCLUSION:The depth of invasion was the only independent risk factor for lymph node metastases.Risk factors for metastases should be considered when choosing surgery for EGC. | Gang Ren Rong Cai Wen-Jie Zhang Jin-Ming Ou Ye-Ning Jin Wen-Hua Li | 2013 | World Journal of Gastroenterology2013，19，20： | 31 |
| 13 | Soil water depletion depth by planted vegetation on the Loess Plateau显示文摘Evapotranspiration of much planted vegetation exceeds precipitation, and this can deplete soil water and cause a deep dry layer in the soil profile, which is a serious obstacle to sustainable land use on the Loess Plateau, China. This study aimed to determine water depletion depth of planted grassland, shrub, and forest in a semiarid area on the Loess Plateau. Soil moisture of five vegetation types was measured to >20 m in depth. The vegetation types were crop, natural grasse, seven-year-old planted alfalfa (Medicago sativa L.), 23-year-old planted caragana (Caragana microphylla Lam.) shrub, and 23-year-old planted pine (Pinus tabulaeformis L) forest land. Through comparing moisture of planted alfalfa grass, caragana shrub, and pine forest to crop and natural grassland, the depth and amount of soil water consumed by grassland, caragana brush and pine forest was determined. The depth of soil water depleted by alfalfa, caragana brush, and pine forest reached 15.5, 22.4 and 21.5 m, respectively. | WANG ZhiQiang LIU BaoYuan LIU Gang ZHANG YongXuan | 2009 | Science China Earth Sciences2009，52，6： | 29 |
| 14 | Expressions of inducible nitric oxide synthase and matrix metalloproteinase-9 and their effects on angiogenesis and progression of hepatocellular carcinoma显示文摘AIM: To determine the expressions of inducible nitric oxide synthase (iNOS) and matrix metalloproteinase-9 (MMP-9)in hepatocellular carcinoma (HCC) and to investigate the relationship between iNOS and MMP-9 expression and their effects on angiogenesis and progression of HCC.METHODS: Tn this study, we examined iNOS, MMP-9,and CD34 expression in specimens surgically removed from 32 HCC patients and 7 normal liver tissues by immunohistochemical staining. Meanwhile, microvessel density (MVD) was determined as a marker of angiogenesis by counting CD34-positive cells.RESULTS: The positive rates of iNOS and MMP-9expression were 71.88% (23/32) and 78.13% (25/32) in HCC. MMP-g expression was significantly correlated with tumor size, capsule status, TNM stage, and risk of HCC recurrence (P= 0.032, P= 0.033, P= 0.007, and P= 0.001,respectively). There was also a significant relationship between iNOS expression and capsule status and risk of HCC recurrence (P = 0.04g and P = 0.004, respectively),but no correlation between iNOS expression and tumor size and TNM stage. There was a positive association between MVD and TNM stage and risk of HCC recurrence (P = 0.037 and P = 0.000, respectively). The count of MVD was significantly different in different iNOS and MMP-9 immunoreactivity groups (F= 17.713 and 17.097, P = 0.000 and P = 0.000, respectively). The examination of Spearman's rank correlation coefficient showed that there was a significant positive correlation between MVD and iNOS,MMP-9 immunoreactivity (r= 0.754 and 0.751, P= 0.000 and P=0.000, respectively). There was also a significant association between MMP-9 and iNOS expression in HCC (P = 0.010).CONCLUSION: Nitric oxide (NO) produced by iNOS could modulate MMP-9 production and therefore contribute to tumor cell angiogenesis and invasion and metastasis in HCC. The strong expression of iNOS and MMP-9 in HCC may be helpful in evaluating the recurrence of HCC,predicting poor prognosis. For patients with strong expression of MMP-9 and iNOS, the optimal treatment scheme needs to be selected. | Min-Hua Sun Xi-Chun Han Ming-Ku Jia Wei-Dong Jiang Min Wang Hong Zhang Gang Han Yi Jiang | 2005 | World Journal of Gastroenterology2005，11，38： | 30 |
| 15 | Heavy metal contamination and source in arid agricultural soil in central Gansu Province,China显示文摘铜( Cu )的集中,铅( Pb ),铬( Cr ),水银( Hg ),和砷(作为)在在 Daba 村庄, Shajiawuan 村庄, Gangou 村庄和 Sifangwu 村庄里收集的干旱农业、灌溉的农业土壤被测量,在中央 Gansu 省定位了,除了 Hg 和 Pb 的 China.Concentrations 比在在选择干旱农业 soils.Pb 集中的灰石灰性土壤的背景价值由结果显示出的72.46% .These 在中国超过干旱农业土壤的阀值低有有 Pb 的确实严肃的污染,为在 Daba 村庄里的灌溉农业土壤的另外的选择金属的一个细微污染问题。种量分析(PCA ) 被用来估计土壤数据,使用有凯撒正规化的 varimax 旋转。结果证明灌溉因素,农业因素和人为的因素都贡献了在选择化学特性之间的关系。Cu 的累积的主要因素, Pb, Cr, Hg 并且这是石版印刷在干旱农业区域的逻辑因素。有 Cu 的起源的惹人注目的不同, Pb, Cr, Hg 并且作为在在 irrigate 农业和干旱农业之间的农业土壤。 | LI Yu GOU Xin WANG Gang ZHANG Qiang SU Qiong XIAO Guoju | 2008 | Journal of Environmental Sciences2008，20，5： | 27 |
| 16 | Multicenter follow-up study of ankle fracture surgery显示文摘在从流行病学、临床的大 multicenter 的中国的脚关节破裂上的很少数据学习的背景是这研究的 available.The 目的是由考察在这研究包括了的 China.Methods 在五所医院经历了脚关节骨折外科的 235 个病人评估流行病学的特征和脚关节破裂的外科的结果经历了脚关节的病人折断外科在到2009年7月 .Age 的从2000年1月的五所中国医院,性,损害的机制, Arbeitsgemeinschaft f | XU Hai-lin LIU Li-min LI Xuan ZHANG Dian-ying FU Zhong-guo WANG Tian-bing ZHANG Pei-xun JIANG Bao-guo SHEN Hui-liang WANG Gang WANG Guang-lin WU Xin-bao | 2012 | Chinese Medical Journal2012，，4： | 27 |
| 17 | OsCERK1-Mediated Chitin Perception and Immune Signaling Requires Receptor-like Cytoplasmic Kinase 185 to Activate an MAPK Cascade in Rice显示文摘保存联系病原体的分子的模式(PAMP ) 例如几丁质,被在激活 mitogen 的蛋白质 kinase (MAPK ) 的快速的激活串联的宿主细胞表面和扳机定位的模式识别受体(PRR ) 察觉,它为植物抵抗被要求到病原体。然而,到在植物的 MAPK 激活的从 PAMP 感觉的直接连接仍然保持大部分未知。在这研究,我们发现联系 PRR 的像受体的细胞质的 kinase Oryza sativa RLCK185 播送到通过和 MAPK kinase kinase 的相互作用表明串联的 MAPK 的从 PAMP 受体 OsCERK1 的有免疫力的发信号, OsMAPKKK,它是 MAPK 串联的起始的 kinase。OsRLCK185 交往与并且 phosphorylates OsMAPKKK 的 C 终端规章的领域。phosphomimetic OsRLCK185 和 OsMAPKKK 的 Coexpression 在 Nicotiana benthamiana 叶子激活 MAPK3/6 phosphorylation。而且, OsMAPKKK 交往与并且 phosphorylates OsMKK4,关键 MAPK kinase 那 transduces 几丁质信号。OsMAPKKK 的 Overexpression 增加导致几丁质的 MAPK3/6 激活,而 OsMAPKKK 击倒的妥协导致几丁质的 MAPK3/6 激活和抵抗到米饭强风真菌。一起拿,我们的结果在米饭从房间表面几丁质感觉建议一条发信号 phospho 小径的存在到 MAPK 串联的细胞内部的激活。 | Chao Wang Gang Wang Chi Zhang Pinkuan Zhu Huiling Dai Nan Yu Zuhua He Ling Xu Ertao Wang | 2017 | Molecular Plant2017，10，4： | 27 |
| 18 | Early Neurological Deterioration after Recanalization Treatment in Patients with Acute Ischemic Stroke: A Retrospective Study显示文摘 | Ying-Bo Zhang Ying-Ying Su Yan-Bo He Yi-Fei Liu Gang Liu Lin-Lin Fan | 2018 | Chinese Medical Journal2018，，2： | 28 |
| 19 | Wnt/β-catenin signalling:function,biological mechanisms,and therapeutic opportunities显示文摘The Wnt/β-catenin pathway comprises a family of proteins that play critical roles in embryonic development and adult tissue homeostasis.The deregulation of Wnt/β-catenin signalling often leads to various serious diseases,including cancer and non-cancer diseases.Although many articles have reviewed Wnt/β-catenin from various aspects,a systematic review encompassing the origin,composition,function,and clinical trials of the Wnt/β-catenin signalling pathway in tumour and diseases is lacking.In this article,we comprehensively review the Wnt/β-catenin pathway from the above five aspects in combination with the latest research.Finally,we propose challenges and opportunities for the development of small-molecular compounds targeting the Wnt signalling pathway in disease treatment. | Jiaqi Liu Qing Xiao Jiani Xiao Chenxi Niu Yuanyuan Li Xiaojun Zhang Zhengwei Zhou Guang Shu Gang Yin | 2022 | Signal Transduction and Targeted Therapy2022，7，2： | 28 |
| 20 | Early diversification of birds: Evidence from a new opposite bird显示文摘A new enantiornithine bird Longipteryx chao-yangensis gen. et sp. nov. is described from the Early Cretaceous Jiufotang Formation in Chaoyang, western Liaoning Province. This new bird is distinguishable from other known enantiornithines in having uncinate processes in ribs, elongate jaws, relatively long wings and short hindlimbs, and metatarsal Ⅳ longer than metatarsals Ⅱ and Ⅲ. This new bird had probably possessed (i) modern bird-like thorax which provides firm attachment for muscles and indicates powerful and active respiratory ability; (ii) powerful flying ability; (iii) special adaptation for feeding on aquatic preys; and (iv) trochleae of metatarsals Ⅰ-Ⅳ almost on the same level, an adaptation for perching. The new bird represents a new ecological type different from all known members of Enantiornithes. It shows that enantiornithines had probably originated earlier than the Early Cretaceous, or this group had experienced a rapid radiation right after it first occurred in the early Early | ZHANG Fucheng ZHOU Zhonghe HOU Lianhai GU Gang | 2001 | Chinese Science Bulletin2001，46，11： | 26 |
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