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| 1 | Simultaneous determination of atorvastatin,metformin and glimepiride in human plasma by LC-MS/MS and its application to a human pharmacokinetic study显示文摘A simple, rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/ MS) assay method has been developed and fully validated for the simultaneous quantification of atorvastatin, metformin and glimepiride in human plasma. Carbamazepine was used as internal standard (IS). The analytes were extracted from 200 mL aliquots of human plasma via protein precipitation using acetonitrile. The reconstituted samples were chromatographed on a Alltima HP C18 column by using a 60:40 (v/v) mixture of acetonitrile and 10 mM ammonium acetate (pH 3.0) as the mobile phase at a flow rate of 1.1 mL/min. The calibration curves obtained were linear (r 2 Z0.99) over the concentration range of 0.50-150.03 ng/mL for atorvastatin, 12.14-1207.50 ng/mL for metformin and 4.98-494.29 ng/mL for glimepiride. The API-4000 LC-MS/MS in multiple reaction monitoring (MRM) mode was used for detection. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. All the analytes were found to be stable in a battery of stability studies. The method is precise and sensitive enough for its intended purpose. A run time of 2.5 min for each sample made it possible to analyze more than 300 plasma samples per day. The developed assay method was successfully applied to a pharmacokinetic study in human male volunteers. | Srinivasa Rao Polagani Nageswara Rao Pilli Ramakrishna Gajula Venkateswarlu Gandu | 2013 | Journal of Pharmaceutical Analysis2013，3，1： | 3 |
| 2 | A rapid and sensitive liquid chromatography-tandem mass spectrometric assay for duloxetine in human plasma:Its pharmacokinetic application显示文摘This paper describes a simple, rapid and sensitive liquid chromatography-tandem mass spectrometry assay for the determination of duloxetine in human plasma. A duloxetine stable labeled isotope (duloxetine d 5 ) was used as an internal standard. Analyte and the internal standard were extracted from 100 mL of human plasma via solid phase extraction technique using Oasis HLB cartridges. The chromatographic separation was achieved on a C 18 column by using a mixture of acetonitrile-5 mM ammonium acetate buffer (83:17, v/v) as the mobile phase at a flow rate of 0.9 mL/min. The calibration curve obtained was linear (r 2 Z0.99) over the concentration range of 0.05-101 ng/mL. Multiple-reaction monitoring mode (MRM) was used for quantification of ion transitions at m/z 298.3/154.1 and 303.3/159.1 for the drug and the internal standard, respectively. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies. | Ramakrishna Gajula Rambabu Maddela Vasu Babu Ravi Jaswanth Kumar Inamadugu Nageswara Rao Pilli | 2013 | Journal of Pharmaceutical Analysis2013，3，1： | 3 |
| 3 | Endoclips for GI endoscopy显示文摘 | Gottumukkala S. Raju Leka Gajula | 2004 | Gastrointestinal Endoscopy2004，，2： | 2 |
| 4 | Endoclips for GI endoscopy显示文摘 | RAJU G S GAJULA L | 2004 | Gas- trointest Endosc2004，59，2： | 1 |
| 5 | MORC2 Signaling Integrates Phosphorylation-Dependent, ATPase-Coupled Chromatin Remodeling during the DNA Damage Response显示文摘 | Da-Qiang Li Sujit S. Nair Kazufumi Ohshiro Anupam Kumar Vasudha S. Nair Suresh B. Pakala Sirigiri Divijendra Natha Reddy Rajendra P. Gajula Jeyanthy Eswaran L. Aravind Rakesh Kumar | 2012 | Cell Reports2012，，6： | 1 |
| 6 | Endoclips for GI endoscope显示文摘 | Raju GS Gajula L | 2012 | Gas- trointest Endosc2012，59，2： | 1 |
| 7 | Pre-fibrillar alpha-synuclein mutants cause parkinson's disease-like non-motor symptoms in drosophila 显示文摘 | GAJULA BALIJA M B GRIESINGER C HERZIG A | 2011 | PLoS One2011，6，24： | 1 |
| 8 | Fermi level de-pinning of aluminum contacts to n-type germanium using thin atomic layer deposited layers显示文摘 | GAJULA D R BAINE P MODREANU M | 2014 | Appl Phys Lett2014，104，01： | 1 |
| 9 | Endoclips for GI endoscopy显示文摘 | Gajula L | 2004 | Gastrointest Endosc2004，59，2： | 1 |
| 10 | A Twist1-MLL-WDR5-HOTTIP complex regulates HOXA9 chromatin to facilitate metastasis of prostate cancer显示文摘 | Tran PT Gajula R Williams R | 2014 | Int J Radiat Oncol Biol Phys2014，90，1： | 1 |
| 11 | A synthetic dolastatin10 analogue suppresses microtubule dynamics, inhibitscell proliferation, and induces apoptotic cell death 显示文摘 | Gajula PK Asthana J Panda D | 2013 | JMed Chem2013，56，6： | 1 |
| 12 | Ef- fect of supplemental inorganic Zn and Mn and their interac- tions on the performance of broiler chicken, mineral bioavailabil- ity,and immune response显示文摘 | GAJULA S S CHELASANI V K PANDA A K | 2011 | Biological Trace Element Research2011，139，2： | 1 |
| 13 | Endoclips for GI endoscopy 显示文摘 | Raju GS Gajula L | 2004 | Gastrointest En- dosc2004，59，2： | 1 |
| 14 | Precooked bran-enriched wheat flour using extrusion:dietary fiber profile andsensory characteristics显示文摘 | Gajula H Alavi S Adhikari K | 2008 | Journal of the Science of FoodScience2008，4，73： | 1 |
| 15 | Endoclips for Gl endoscopy 显示文摘 | Raju GS Gajula L | 2004 | GastrointestEndosc2004，59，2： | 1 |
| 16 | APRI: An Easy and Validated Predictor of Hepatic Fibrosis in Chronic Hepatitis C显示文摘 | Ned Snyder Leka Gajula Shu-Yuan Xiao James Grady Bruce Luxon Daryl T-Y Lau Roger Soloway John Petersen | 2006 | Journal of Clinical Gastroenterology2006，，6： | 1 |
| 17 | Endoclips for GI endoscopy显示文摘 | Raju GS Gajula L | 2004 | Gastrointest Endosc2004，59，2： | 1 |
| 18 | Enhancement of solvent uptake in porous PVDF nanofibers derived by a water-mediated electrospinning technique显示文摘The effect of a N,N-dimethylformamide(DMF)/acetone solvent system(3:7,4:6,5:5,6:4,7:3)and spinning medium(air and water)on the membrane morphology and the structure-property relationship were investigated.A facile method was optimized to generate a porous,polymer-fiber membrane via the combinative effect of electrospinning and thermally inducing phase separation of the DMF/acetone(4:6)solvent system in a water medium.The attenuated total reflection(ATR)-Fourier transform infrared(FTIR)results showed an increased b-phase compared to the pristine poly(vinylidene fluoride)(PVDF).The XRD and DSC results further confirmed that the co-existing a-and b-phases in the pristine PVDF were converted into a unique b-phase in the electrospun membranes.In addition,the solvent uptake percentage of the DMF/acetone(4:6)solvent system in a water medium(540)is much greater than that in an air medium(320),and over two times better than that of commercial polyethylene(PE)membranes(190).Similarly,the discharge capacity of the PVDF membrane separator prepared with the DMF/acetone(4:6)solvent system in a water medium is higher than that of the air medium.This enhancement of solvent uptake might be due to the interconnected porous morphology present in the water medium. | Gajula Prasad Jun-Wei Liang Wei Zhao Yingbang Yao Tao Tao Bo Liang Sheng-Guo Lu | 2021 | Journal of Materiomics2021，7，2： | 1 |
| 19 | Endoclips for GI endoscopy显示文摘 | Raju GS Gajula L | 2004 | Gastroint- est Endosc2004，59，2： | 1 |
| 20 | Endoclips for GI endoscopy显示文摘 | RAJU GS GAJULA L | 2004 | Gastrointest Endosc2004，59，2： | 1 |
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