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| 1 | Genetic basis and breeding application of clonal diversity and dual reproduction modes in polyploid Carassius auratus gibelio显示文摘A unisexual species is generally associated with polyploidy, and reproduced by a unisexual reproduction mode, such as gyno- genesis, hybridogenesis or parthenogenesis. Compared with other unisexual and polyploid species, gibel carp (Carassius au- ratus gibelio) has a higher ploidy level of hexaploid. It has undergone several successive rounds of genome polyploidy, and experienced an additional, more recent genome duplication event. More significantly, the dual reproduction modes, including gynogenesis and sexual reproduction, have been demonstrated to coexist in the polyploid gibel carp. This article reviews the genetic basis concerning polyploidy origin, clonal diversity and dual reproduction modes, and outlines the progress in new va- riety breeding and gene identification involved in the reproduction and early development. The data suggests that gibel carp are under an evolutionary trajectory of diploidization. As a novel evolutionary developmental (Evo-Devo) biology model, this work highlights future perspectives about the functional divergence of duplicated genes and the sexual origin of vertebrate animals. | GUI JianFang* & ZHOU Li State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China | 2010 | Science China(Life Sciences)2010，53，4： | 62 |
| 2 | Molecular basis and genetic improvement of economically important traits in aquaculture animals显示文摘Aquaculture has been believed to be a major Chinese contribution to the world. In recent 20 years, genome and other genetic technologies have promoted significant advances in basic studies on molecular basis and genetic improvement of aquaculture animals, and complete genomes of some main aquaculture animals have been sequenced or announced to be sequenced since the beginning of this century. Here, we review some significant breakthrough progress of aquaculture genetic improvement technologies including genome technologies, somatic cell nuclear transfer and stem cell technologies, outline the molecular basis of several economically important traits including reproduction, sex, growth, disease resistance, cold tolerance and hypoxia tolerance, and present a series of candidate trait-related genes. Finally, some application cases of genetic improvement are introduced in aquaculture animals, especially in China, and several development trends are highlighted in the near future. | GUI JianFang ZHU ZuoYan | 2012 | Chinese Science Bulletin2012，57，15： | 49 |
| 3 | Detection of Grass Carp Hemorrhage Virus (GCHV) from Vietnam and Comparison with GCHV Strain from China显示文摘Grass carp plays an important role in small-scale aquaculture in Vietnam. However, a severe disease, known in Vietnam as 'Red Spot Disease', is causing significant economic loss in grass carp aquaculture. In this study, the tissue samples isolated from the grass carp with Red Spot Disease in Vietnam are investigated and comparied with the control GCHV isolated in China by experimental infection, culture cell infection, serological cross reactivity, and RT-PCR amplification. Infected grass carp exhibits hemorrhage symptoms about 5 days after experimental injection with GCHV-V (Vietnam) strain. The symptoms and lethality induced by the GCHV-V strain are identical to that induced by the Chinese GCHV-9014 strain. The Chinese GCHV-873 strain induces typical cytopathogenic effects in 4 cell lines, such as CIK, CAB, FHM and GCO, from the 6 fish cell lines examined. No cytopathogenic effects are observed in all the 6 examined cell lines, including CAB, FHM, CIK, EPC, CCO and GCO, infected by the GCHV-V strain and GCHV-9014 strain. Immunodiffusion assays demonstrate an obvious cross-reactivity among three GCHV strains. Precipitin lines are clearly observed not only between the anti-GCHV-873 serum and the two strains GCHV-873 and GCHV-9014, but also between the anti-GCHV-873 serum and the GCHV-V strain. GCHV can be detected by immunodiffusion assays after three generations of blind propagations in the cell lines inoculated by GCHV-V strain. This implicates that GCHV-V viruses have been replicated and amplified despite there being no cytopathogenic effects observed in these examined cell lines. Three genome segments of GCHV, including S8, S9 and S10, are amplified by three sets of PCR primers designed according to the segment sequences published recently. The Q8fp and Q8rp primer set specific for genome segment S8 amplifies a 955 bp fragment from the extracted sample of diseased fish with Red Spot Disease, and the fragment size is identical to that amplified by the same primer set from control GCHV-873 strain. Simultaneously, the Q9fp and Q9rp primer set specific for genome segment S9 generates a same 635 bp product, and the Q10fp and Q10rp primer set specific for genome segment S10 produces a same 697 bp fragment from both template samples of diseased fish with Red Spot Disease and control GCHV-873 strain. The RT-PCR amplification and corresponding size comparison data indicate that the three GCHV-V genome segments extracted from the diseased grass carp with Red Spot Disease in Vietnam should be identical to that in control GCHV-873 strain from China. The data confirm that the causative agent of grass carp Red Spot Disease in Vietnam is a virus, and the virus is closely similar to GCHV strain in China. | 张奇亚 Ruan Hongmei Li Zhenqiu Zhang Jing Gui Jianfang | 2003 | High Technology Letters2003，9，2： | 20 |
| 4 | Natural and artificial polyploids in aquaculture显示文摘Genome polyploidy has been revealed to result in evolutionary advantages and novelties,and therefore,polyploid aquatic animals may possess excellent traits of economic interest including rapid growth,extensive adaptability and disease resistance.For this reason,numerous species of natural polyploid fishes,such as common carp,gibel carp,crucian carp,salmon,and sturgeon,were chosen as important target species for aquaculture.Many artificial polyploids have been commercially utilized for aquaculture and most of them were created from natural polyploid fishes of the Cyprinidae and Salmonidae.Thanks to the easy mass production and better economic traits in growth and flesh quality,the synthetized autopolyploids or allopolyploids from natural polyploid species in cyprinid fishes have been extensively applied to aquaculture throughout China.This review outlines polyploidy advantages and innovative opportunities,lists natural polyploid species used in aquaculture,and summarizes artificial polyploids that have been induced or synthetized,and used in aquaculture.Moreover,some main research trends on polyploid utilization and ploidy manipulation of aquaculture animals are also introduced and discussed in the review. | Li Zhou Jianfang Gui | 2017 | Aquaculture and Fisheries2017，2，3： | 16 |
| 5 | Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio显示文摘Gibel 鲤鱼(Carassius auratus gibelio ) 是一特别地有在自然产地的男性的次要的比率的 gynogenetic 种类,而是它的男起源和性决心机制是未知的。在这研究,一个偏导男性的变异的家庭从 gynogenetic gibel 鲤鱼被发现,并且一个男性特定的疤标记从变异的家庭被识别。正常精子发生被免疫荧光组织化学在男睾丸观察。将近相同的 AFLP 侧面在男性和女性之间被观察,但是一男性特定 86 bp AFLP 碎片被性水池 bulked segregant 分析并且单个屏蔽屏蔽。基于男性特定的 AFLP 碎片, 579 个 bp 序列的一个总数被染色体走克隆。随后,一个男性特定的疤标记被设计,并且男性特定的 DNA 碎片被证实稳定地被传给下一代并且一致地仅仅在男性检测了。 | Da Wang Huiling Mao Jinxia Peng Xiyin Li Li Zhou Jianfang Gui | 2009 | Progress in Natural Science:Materials International2009，19，11： | 11 |
| 6 | Phenotypic and genetic variation between two populations of the Chinese yellow pond turtle, Mauremys mutica (Cantor, 1842)显示文摘Mauremys mutica(Cantor,1842)is an endangered species in China.Main phenotypic variations inbody color,body weight,body shape,clutch size,egg size,and hatchling size were revealed betweenthe southern and northern populations.Both populations have the phenomenon of'larger male'sexualsize dimorphism(SSD),especially in the southern population.Furthermore,genetic variations betweenthe two populations were analyzed by RAPD band patterns of 30 random individuals in each population.The average genetic distance was 0.299±0.108 among the samples of two populations.The average ge-netic distance between southern and northern populations was 0.305±0.046.Cluster analysis indicatedthat all the individuals from the southern and northern populations were clustered among themselves toform two distinct clades.A total of 20 population-specific RAPD fragments were scored from 16 primers,and could be used as RAPD markers for distinguishing the southern and the northern population.Basedon the nucleotide sequences of two RAPD markers,two pairs of SCAR primers(SC1-S and SC2-S)weredesigned,which could be used as SCAR markers for the southern population.According to the significantphenotypic and genetic variations,we suggested that the northern population and southern populationmight be considered as two separate taxa,the'northern taxon'and the'southern taxon',and the con-servation should be respectively conducted on the two taxa. | 朱新平 Zhou Li Chen Yongle Du Hejun Gui Jianfang | 2008 | High Technology Letters2008，14，1： | 10 |
| 7 | Sex differences in the expression of GH/IGF axis genes underlie sexual size dimorphism in the yellow catfish(Pelteobagrus fulvidraco)显示文摘Dear Editors,Sexual dimorphism is the systematic difference in size,shape,color,physiology,and behavior,between male and female individuals of the same species(Mei and Gui,2015).Some studies have indicated that the traits of sexual dimorphism in vertebrates are the consequences of sex-biased gene expression and are controlled by multiple critical genes during growth and development(Williams and Carroll,2009).However,the exact molecular mechanism underlying sexual dimorphism remains unclear.Sexual dimorphism with respect to size has been observed in | Wenge Ma Junjie Wu Jin Zhang Yan He Jianfang Gui Jie Mei | 2016 | Science China(Life Sciences)2016，59，4： | 7 |
| 8 | Bioinformatic identification of genes encoding Clq-domaincontaining proteins in zebrafish显示文摘C1q is the first subcomponent of classical pathway in the complement system and a major link between innate and acquired immuni- ties. The globular (gC1q) domain similar with C1q was also found in many non-complement C1q-domain-containing (C1qDC) proteins which have similar crystal structure to that of the multifunctional tumor necrosis factor (TNF) ligand family, and also have diverse func- tions. In this study, we identified a total of 52 independent gene sequences encoding C1q-domain-containing proteins through compre- hensive searches of zebrafish genome, cDNA and EST databases. In comparison to 31 orthologous genes in human and different numbers in other species, a significant selective pressure was suggested during vertebrate evolution. Domain organization of C1q-domain-con- taining (C1qDC) proteins mainly includes a leading signal peptide, a collagen-like region of variable length, and a C-terminal C1q do- main. There are 11 highly conserved residues within the C1q domain, among which 2 are invariant within the zebrafish gene set. A more extensive database searches also revealed homologous C1qDC proteins in other vertebrates, invertebrates and even bacterium, but no homologous sequences for encoding C1qDC proteins were found in many species that have a more recent evolutionary history with zebrafish. Therefore, further studies on C1q-domain-containing genes among different species will help us understand evolutionary mechanism of innate and acquired immunities. | Jie Mei Jianfang Gui | 2008 | Journal of Genetics and Genomics2008，35，1： | 7 |
| 9 | Differential gene expression of protein kinases in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp显示文摘A modified mRNA differential display method has been applied to studying differential expression of protein kinase genes in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp. Total RNA was reverse transcribed using downstream 3′primers T12MA, T12MG and T12MC respectively. Then the reverse transcription products were amplified using upstream 5′kinase-specific primer designed according to protein kinase conserved sequence. The PCR products had different patterns and numbers of cDNA bands on polyacrylamide gel. Totally 21 cDNAs fragments were recovered and cloned. Two of them were confirmed to be particularly expressed in oocytes of amphimictic crucian carp, and another was specific for gynogenetic silver crucian carp. | Jing Xie Yan Zhu Fan Zhang Jianfang Gui | 1999 | Chinese Science Bulletin1999，44，14： | 5 |
| 10 | Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish显示文摘Apolipoprotein C1(Apoc1)is associated with lipoprotein metabolism,but its physiological role during embryogenesis is largely unknown.We reveal a new function of Apoc1b,a transcript isoform of Apoc1,in epiboly during zebrafish gastrulation.Apoc1b is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos.It displays a radial gradient with high levels in the interior layer and low levels in the superficial layer.Knockdown of Apoc1b by injecting antisense morpholino(MO)caused the epiboly arrest in deep cells.Moreover,we show that the radial intercalation and the radial gradient distribution of E-cadherin are disrupted both in Apoc1b knockdown and overexpressed embryos.Therefore,Apoc1b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner. | WANG Yang ZHOU Li LI Zhi LI WenHua GUI JianFang | 2013 | Science China(Life Sciences)2013，56，11： | 3 |
| 11 | Animal reproduction and physiology: from basis to application显示文摘Animal reproduction and physiology is one of the traditional subjects in biology, and also one of the most rapidly developing fields because it is related to human | GUI JianFang,Prof. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China | 2010 | Science China(Life Sciences)2010，53，4： | 2 |
| 12 | A novel cyanophage with cyanobacterial non-bleaching protein a gene in the genome显示文摘 | GAO E-Bin GUI Jianfang ZHANG Qiya | 2012 | Journal of Virology2012，86，1： | 1 |
| 13 | Transcription of Il17 and Il17f Is Controlled by Conserved Noncoding Sequence 2显示文摘 | Xiaohu Wang Yibing Zhang Xuexian O. Yang Roza I. Nurieva Seon Hee Chang Sandra S. Ojeda Hong S. Kang Kimberly S. Schluns Jianfang Gui Anton M. Jetten Chen Dong | 2012 | Immunity2012，，： | 1 |
| 14 | Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder,Paralichthys olivaceus显示文摘The double-stranded RNA(dsRNA)-dependent protein kinase(PKR) belongs to the eIF2a kinase family and plays a critical role in interferon(IFN)-mediated antiviral response.Recently,in Japanese flounder(Paralichthys olivaceus),a PKR gene has been identified.In this study,we showed that PoPKR localized to the cytoplasm,and the dsRNA-binding motifs(dsRBMs) played a determinative role in protein localization.In cultured FEC cells,PoPKR was detected at a low level of constitutive expression but was highly induced after treatment with UV-inactivated grass carp hemorrhagic virus,active SMRV and Poly I:C although with different expression kinetics.In flounder,PoPKR was ubiquitously distributed in all tested tissues,and SMRV infection resulted in significant upregulation at mRNA and protein levels.In order to reveal the role of PoPKR in host antiviral response,its expression upon exposure to various inducers was characterized and further compared with that of PoHRI,which is another eIF2a kinase of flounder.Interestingly,expression comparison revealed that all inducers stimulated upregulation of PoHRI in cultured flounder embryonic cells and fish,with a similar kinetics to PoP-KR but to a less extent.These results suggest that,during antiviral immune response,both flounder eIF2a kinases might play similar roles and that PoPKR is the predominant kinase. | Rong Zhu Yibing Zhang Qiya Zhang Jianfang Gui | 2009 | Progress in Natural Science:Materials International2009，19，10： | 1 |
| 15 | Staphylococcus aureus lipoproteins trigger human corneal epithelial in-nate response through toll-like receptor-2 显示文摘 | Li Qiong Kumar A Gui Jianfang | 2008 | Microbial Pathogenesis2008，44，5： | 1 |
| 16 | Hyperandrogenism in POMCa-deficient zebrafish enhances somatic growth without increasing adiposity显示文摘The endocrine regulatory roles of the hypothalamic-pituitary-adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa.However,the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone(ACTH),a pro-opiomelanocortin(POMC)-derived peptide,on animal energy expenditure and somatic growth remain unknown.POMC has also been identified as one of the candidate loci for polycystic ovary syndrome,which features hyperandrogenism and some prevalence of obesity in patients.Here we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian models.In contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin,which are responsible for their obesity-prone weight gain observed in various pome mutant mammals,analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient zebrafish.Hypocortisolism associated with hyperandrogenism has been observed in the pomca-deficient zebrafish,with enhanced activation of mammalian target of rapamycin complex 1;reutilization of amino acids and fatty acid^-oxidation are observed in the muscle tissue of the pomca-deficient fish.After reducing hyperandrogenism by crossing our pomca mutant fish with a cy p l 7a 1-deficient background,the phenotype of enhanced somatic growth in pomca-deficient fish was no longer observed.Thus,our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates,whereas its effect on adipostasis is unique to teleosts. | Chuang Shi Yao Lu Gang Zhai Jianfei Huang Guohui Shang Qiyong Lou Dongliang Li Xia Jin Jiangyan He Zhenyu Du Jianfang Gui Zhan Yin | 2020 | Journal of Molecular Cell Biology2020，12，4： | 1 |
| 17 | Retention of the Developmental Pluripotency in Medaka Embryonic Stem Cells after Gene Transfer and Long-term Drug Selection for Gene Targeting in Fish显示文摘 | Yunhan Hong Songlin Chen Jianfang Gui Manfred Schartl | 2004 | Transgenic Research2004，，1： | 1 |
| 18 | In vitro culture of embryonic hearts from guppy fish (Poecilia reticulata)显示文摘Forty embryonic hearts were taken out by anatomical needle from denuded embryos of the ovovivipar-ity guppy fish that were dechorioned by mechanic method or by trypsin digestion, and were in vitro cultured. In the cultured hearts, 80% have maintained beating in vitro for 4 weeks, and the longest record for beating was 142 d. Owing to fish embryo transparency, beating frequency and blood color changes are easily viewed from the embryonic hearts under a dissecting microscope. The current study established the in vitro culture method of embryonic hearts in guppy fish, which can be used as a model for the study of heart and cardiovascular system in vertebrates. | Qiya Zhang Zhenqiu Li Jianfang Gui | 2001 | Chinese Science Bulletin2001，46，19： | 0 |
| 19 | Genomic polymorphisms at the crhr2 locus improve feed conversion efficiency through alleviation of hypothalamus-pituitary-interrenal axis activity in gibel carp(Carassius gibelio)显示文摘Improvement in fish feed conversion efficiency(FCE)is beneficial for sustaining global food fish supplies.Here,we show that a set of polymorphisms at locus of the corticotropin releasing hormone receptor 2(crhr2),which is involved in hypothalamuspituitary-interrenal(HPI)axis signaling,is associated with improved FCE in farmed allogynogenetic gibel carp strain CAS Ⅲ compared with that in the wild gibel carp strain Dongting(DT).This set of polymorphisms downregulates the expression levels of crhr2 mRNA in the brain and pituitary tissues in gibel carp strain CAS Ⅲ compared with those in strain DT.Furthermore,compromised HPI axis signaling is observed in gibel carp strain CAS Ⅲ,such as decreased α-melanocyte stimulating hormone protein levels,plasma cortisol content,and stress responses.Moreover,enhanced activation of protein kinase B/mammalian target of rapamycin complex 1 signaling observed in the muscle tissue of strain CAS Ⅲ in comparison to that in strain DT indicated elevated anabolic metabolism in strain CAS Ⅲ.Thus,these studies demonstrate that the genetic markers associated with compromised HPI axis signaling,such as crhr2,are potentially useful for genetic selection toward improvement in farmed fish growth and FCE,which would reduce fishmeal consumption and thereby indirectly facilitate sustainable fisheries. | Chuang Shi Qiyong Lou Beide Fu Junyan Jin Jianfei Huang Yao Lu Xia Jin Jiangyan He Gang Zhai Shouqi Xie Jingou Tong Jianfang Gui Zhan Yin | 2022 | Science China(Life Sciences)2022，65，1： | 0 |
| 20 | Genetic difference of Chinese horseshoe crab(Tachypleus tridentatus) in southeast coast of China based on mitochondrial COI gene analysis显示文摘Population genetic structure and historical demography of Chinese horseshoe crab(T.tridentatus) along southeast coast of China were inferred from the sequence data of mitochondrial cytochrome c oxidase subunit I(COI) fragment.The sequence analysis for 964 bp COI fragment was conducted in 28 individuals collected from five localities:Ninghai in Zhejiang Province,Meizhou and Zhangpu in Fujian Province,Beihai of Guangxi Zhuang Autonomous Region and Danzhou of Hainan Province.Sequence variation was relatively low with a total of seven transitions observed.In all localities,Haplotype H3 was the dominant type observed among eight haplotypes defined previously,and was at the center of radiation in Median-Joining network.The prolonged star-like network suggests a signature of population expansions.The level of diversity was low in total,with haplotype diversity(H d) being equal to 0.765 and nucleotide diversity(π) being equal to 0.001 18,respectively.The genetic structure analysis revealed the significant genetic difference between Ninghai and Danzhou populations.Both mismatch distribution analysis and Fu's F s test provided consistent inference of historic population expansion.The low genetic diversity of horseshoe crab observed along China coast indicated that urgent measures should be taken to protect this rare marine animal. | WENG Zhaohong XIAO Zhiqun XIE Yangjie WANG Zhiyong GUI Jianfang | 2012 | Acta Oceanologica Sinica2012，31，3： | 0 |
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