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| 1 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 2 | Diagnosis and treatment recommendations for pediatric respiratory infection caused by the 2019 novel coronavirus显示文摘Since December 2019,an epidemic caused by novel coronavirus (2019-nCoV) infection has occurred unexpectedly in China.As of 8 pm,31 January 2020,more than 20 pediatric cases have been reported in China.Of these cases,ten patients were identified in Zhejiang Province,with an age of onset ranging from 112 days to 17 years.Following the latest National recommendations for diagnosis and treatment of pneumonia caused by 2019-nCo V (the 4th edition) and current status of clinical practice in Zhejiang Province,recommendations for the diagnosis and treatment of respiratory infection caused by 2019-nCoV for children were drafted by the National Clinical Research Center for Child Health,the National Children's Regional Medical Center,Children's Hospital,Zhejiang University School of Medicine to further standardize the protocol for diagnosis and treatment of respiratory infection in children caused by 2019-nCoV. | Zhi-Min Chen Jun-Fen Fu Qiang Shu Ying-Hu Chen Chun-Zhen Hua Fu-Bang Li Ru Lin Lan-Fang Tang Tian-Lin Wang Wei Wang Ying-Shuo Wang Wei-Ze Xu Zi-Hao Yang Sheng Ye Tian-Ming Yuan Chen-Mei Zhang Yuan-Yuan Zhang | 2020 | World Journal of Pediatrics2020，16，3： | 45 |
| 3 | Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis显示文摘在哺乳动物的精子发生被 piRNA 表示的二个波浪描绘:一个人对应于负责的经典 piRNAs 因为 silencing retrotransponsons 和第二个波浪主要在 pachytene spermatocytes 从 nontransposon intergenic 区域被导出,但是这些 pachytene piRNAs 的功能大部分是未知的。这里,我们在在老鼠伸长指示巨大的 mRNA 消除报导 pachytene piRNAs 的参与 spermatids (ES ) 。我们证明包含鼠科的 PIWI (MIWI ) 和 deadenylase CAF1 的导致 piRNA 的 silencing 建筑群(pi-RISC ) 有选择地在 ES 被装配,它负责经由在体的房间类似于 miRNAs 的行动的机制导致 mRNA deadenylation 和腐烂。如此的一个高度安排的节目看起来充分利用 pachytene piRNAs 的能力从 nontransposon intergenic 区域导出的多样化的指向的庞大的全部剧目。这些调查结果建议 pachytene piRNAs 负责为精子生产作准备,从 ES 使广阔细胞的节目失去活性。 | Lan-Tao Gou Peng Dai Jian-Hua Yang Yuanchao Xue Yun-Ping Hu Yu Zhou Jun-Yan Kang Xin Wang Hairi Li Min-Min Hua Shuang Zhao Si-Da Hu Li-Gang Wu Hui-Juan Shi Yong Li Xiang-Dong Fu Liang-Hu Qu En-Duo Wang Mo-Fang Liu | 2014 | Cell Research2014，24，6： | 39 |
| 4 | A mouse model for HBV immunotolerance and immunotherapy显示文摘一个适当小动物模型的缺乏仍然是一个主要障碍因为学习 immunotolerance 和 immunopathogenesis 由肝炎 B 病毒(HBV ) 导致了感染。在这研究,我们与 recombinant 在感染以后与持续 HBV viremia 报导一个老鼠模型带一个可复制的 HBV 染色体(AAV/HBV ) 的联系 adeno 的病毒(AAV ) 。类似于临床的 HBV 搬运人,感染 AAV/HBV 的老鼠为对 HBV 表面抗原(HBsAg ) 的抗体是 sero 否定的。有面对铝助手疫苗的常规 HBV 的免疫没能在这些老鼠对 HBV 得到有免疫力的回答。为了识别一支疫苗,那能潜在地围绕这忍耐, TLR9 收缩筋 CpG 作为一个助手被加到 HBsAg。有 HBsAg/CpG 的老鼠的种痘导致了 viremia,而且强壮的抗体生产和 T 房间回答的不仅清理。而且, DNA 复制和 HBV 的蛋白质表示显著地在 AAV/HBV-infected 鼠标的肝被减少。因此, AAV/HBV-infected 老鼠可以作为一个柔韧的模型被使用调查 HBV immunotolerance 并且为开发新奇免疫疗法根除的内在的机制 HBV 感染。 | Dan Yang Longchao Liu Danming Zhu Hua Peng Lishan Su Yang-Xin Fu Liguo Zhang | 2014 | Cellular & Molecular Immunology2014，11，1： | 25 |
| 5 | Development of PLC-based Tension Control System显示文摘Fiber winding tension is an important factor in the molding techniques of composite material which influences the quality of winding product directly, and the tension control is a key technique in fiber winding techniques. This paper introduces a closed-loop tension control system with the programmable logic controller (PLC) with function modules as its control kernel, the alternating current (AC) servo motor as execute element and the radius-following device to accomplish the real-time radius compensation. The mechanism of the tension control system is analyzed and the numerical model is set up. The compensation technique of the radius of the scroll is analyzed. Experimental results show that the system is well qualified with high control precision and high reaction speed. | REN Sheng-le LU Hua WANG Yong-zhang FU Hong-ya | 2007 | Chinese Journal of Aeronautics2007，20，3： | 23 |
| 6 | nsights into battles between Mycobacterium tuberculosis and macrophages显示文摘 | Guanghua Xu Jing Wang George Fu Gao Cui Hua Liu | 2014 | Protein & Cell2014，5，10： | 22 |
| 7 | Humoral and cellular immunogenecity of DNA vaccine based on hepatitis B core gene in rhesus monkeys显示文摘INTRODUCTIONHepatitis B virus (HBV) is the most commonetiologic agent for infectious liver diseases. It isestimated that there are more than 250 millionchronic HBV carriersin the world today and thereis a significant association among persistentinfection, liver cirrhosis and hepatocellularcarcinoma[1-3]. | Zu Hu Huang1 Hui Zhuang2 Shan Lu3 Ren Hua Guo1 Guo Min Xu2 Jie Cai1 Wan Fu Zhu2 1Department of Infectious Diseases. The First Affiliated Hospital of Nanjing Medical University, Nenjing 210029, Jiangsu Province. China2Faculty of Microbiology, Beijing University, Beijing 100000, China3University of Massachusetts Medical Center | 2001 | World Journal of Gastroenterology2001，7，1： | 19 |
| 8 | TGF-β promotes invasion and metastasis of gastric cancer cells by increasing fascinl expression via ERK and JNK signal pathways显示文摘 | Hua Fu Zhongliang Hu Jifang Wen Kuansong Wang Ying Liu | 2009 | Acta Biochimica et Biophysica Sinica2009，41，8： | 19 |
| 9 | The efficacy and safety of weekly 35-mg risedronate dosing regimen for Chinese postmenopausal women with osteoporosis or osteopenia: 1-year data显示文摘目的: 当每周,每天管理了时,口头的 risedronate 在绝经后的骨质疏松症的处理是有效的。在这 1 年,使随机化,双窗帘,我们比较了的 multicenter 学习在有骨质疏松症或 osteopenia 的中国绝经后的女人的处理的每周的 35-mg 和每日的 5-mg risedronate dosing 政体。 | Jie-mei GU Li WANG Hua LIN De-cai CHEN Hai TANG Xiao-lan JIN Wei-bo XIA Yun-qiu HU Wen-zhen FU Jin-wei HE Hao ZHANG Chun WANG Hua YUE Wei-wei HU Yu-juan LIU Zhen-lin ZHANG | 2015 | Acta Pharmacologica Sinica2015，36，7： | 19 |
| 10 | Additive Benefits of Twice Forest Bathing Trips in Elderly Patients with Chronic Heart Failure显示文摘长期的心失败(CHF ) ,源于各种各样的心血管的疾病(CVD ) 的后果的临床的症候群,逐渐地正在成为病态和死亡的一个全球原因。我们更早证明了洗旅行的一个 4 天的森林能在病人上向附属治疗学的影响提供 CHF。为了进一步调查影响和森林洗澡的最佳的频率的持续时间,与 CHF 在病人摔倒,我们招募了经历了在 4 个星期以后再洗旅行的第一个森林并且也就是,随机把他们分成了二个组的那些对象城市的控制组(城市)和洗组(森林)的森林。在洗旅行的第二个 4 天的森林以后,我们象氧化应力一样在大脑 natriuretic 肽层次,心失败的 biomarker,和稀释煽动性的回答观察了稳定的衰落。因此,这探索研究表明了添加剂好处两次,森林洗澡与 CHF 在老病人摔倒,它能进一步为在 CVD 分析如此的干预的效果铺平道路。 | MAO Gen Xiang CAO Yong Bao YANG Yan CHEN Zhuo Mei DONG Jian Hua CHEN Sha Sha WU Qing LYU Xiao Ling JIA Bing Bing YAN Jing WANG Guo Fu | 2018 | Biomedical and Environmental Sciences2018，31，2： | 18 |
| 11 | Simvastatin inhibits apoptosis of endothelial cells induced by sepsis through upregulating the expression of Bcl-2 and downregulating Bax显示文摘BACKGROUND: Many studies have showed that apoptosis of endothelial cells plays a curial role in the progress of sepsis. But the role of simvastatin in apoptosis of endothelial cells induced by sepsis is not clear. The present study aimed to investigate the role of simvastatin in apoptosis of endothelial cells induced by sepsis and its mechanism.METHODS: Human umbilical vein endothelial cells(HUVECs) were randomly divided into three groups: control group, sepsis serum intervention group(sepsis group) and simvastatin+sepsis serum intervention group(simvastatin group). After 24-hour incubation with corresponding culture medium, the relative growth rate of HUVECS in different groups was detected by MTT assay; the apoptosis of HUVECs was detected by Hoechst33258 assay and fl ow cytometry; and the expression of the Bcl-2 and Bax genes of HUVECs was detected by PCR.RESULTS: Compared with the sepsis group, HUVECs in the simvastatin group had a higher relative growth rate. Apoptotic HUVECs decreased significantly in the simvastatin group in comparison with the sepsis group. Expression of the Bcl-2 gene in HUVECs decreased obviously, but the expression of the Bax gene increased obviously after 24-hour incubation with sepsis serum; however, the expression of the Bcl-2 and Bax genes was just the opposite in the simvastatin group.CONCLUSIONS: Our study suggests that simvastatin can inhibit apoptosis of endothelial cells induced by sepsis through upregulating the expression of Bcl-2 and downregulating Bax. It may be one of the mechanisms for simvastatin to treat sepsis. | Hui Fu Qiao-sheng Wang Qiong Luo Si Tan Hua Su Shi-lin Tang Zheng-liang Zhao Li-ping Huang | 2014 | World Journal of Emergency Medicine2014，5，4： | 18 |
| 12 | Real-time content-aware image resizing显示文摘Content-aware image resizing is a kind of new and effective approach for image resizing,which pre-serves image content well and does not cause obvious distortion when changing the aspect ratio of images. Recently,a seam based approach for content-aware image resizing was proposed by Avidan and Shamir. Their results are impressive,but because the method uses dynamic programming many times,it is slow. In this paper,we present a more efficient algorithm for seam based content-aware im-age resizing,which searches seams through establishing the matching relation between adjacent rows or columns. We give a linear algorithm to find the optimal matches within a weighted bipartite graph composed of the pixels in adjacent rows or columns. Therefore,our method is fast (e.g. our method needs only about 100 ms to reduce a 768×1024 image’s width to 1/3 while Avidan and Shamir’s method needs 12 s). This supports immediate image resizing whereas Avidan and Shamir’s method requires a more costly pre-processing step to enable subsequent real-time processing. A fast method such as the one proposed will be also needed for future real-time video resizing applications. | HUANG Hua FU TianNan ROSIN Paul L QI Chun | 2009 | Science in China(Series F)2009，52，2： | 17 |
| 13 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 14 | Chemokine ligand 20 enhances progression of hepatocellular carcinoma via epithelial-mesenchymal transition显示文摘AIM: To identify the mechanisms of chemokine ligand 20(CCL20)-induced hepatocellular carcinoma(HCC) metastasis and evaluate it as a prognostic marker. METHODS: Expression of CCL20 was evaluated by immunohistochemistry in HCC tissues from 62 patients who underwent curative resection. The relationship between CCL20 expression and clinicopathologic features was analyzed. Univariate and multivariate analyses were performed to evaluate its predictive value for recurrence and survival of HCC patients. The expression levels ofepithelial-mesenchymal transition(EMT)-and signaling pathway-related proteins were evaluated by Western blotting and immunocytochemistry. The effects of CCL20 on HCC cell proliferation and migration were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenoltetrazolium bromide(MTT) and Transwell assays. RESULTS: CCL20 immunoreactivity was detected in all 62 patient specimens. CCL20 expression was associated with preoperative alpha-fetoprotein level(P = 0.043), tumor size(P = 0.000), tumor number(P = 0.008), vascular invasion(P = 0.014), and tumor differentiation(P = 0.007). Patients with high CCL20 expression had poorer recurrence-free and overall survivals compared to those with low CCL20 expression(both P < 0.001). CCL20 induced EMT-like changes in HCC cells and increased their proliferation and migration ability(P < 0.05). Western blotting and immunofluorescence staining showed that CCL20 induced an EMT-like phenotype in HCC cells, and increased expression of phosphorylated AKT, β-catenin and vimentin, and decreased E-cadherin expression(P < 0.05). The correlation analysis revealed that high CCL20 expression in HCC tissue specimens was negatively correlated with E-cadherin expression(13.33%, 4/30), and positively correlated with vimentin(90.0%, 27/30), β-catenin(96.67%, 29/30) and p-AKT(76.67%, 23/30) expression.CONCLUSION: CCL20 expression is associated with HCC recurrence and patient survival and promotes HCC cell proliferation and migration by inducing EMT-like changes via PI3K/AKT and Wnt/β-catenin pathways. | Ke-Zhu Hou Zhi-Qiang Fu Hua Gong | 2015 | World Journal of Gastroenterology2015，21，2： | 13 |
| 15 | Acupuncture treatment of lung-spleen Qi deficiency in stable chronic obstructive pulmonary disease: a randomized, open-label, controlled trial显示文摘OBJECTIVE:To evaluate the effectiveness and safety of acupuncture that reinforces the spleen to strengthen the lung in patients with stable chronic obstructive pulmonary disease(COPD).METHODS:This was a randomized,open-controlled trial in which the acupuncturist and the participants were not blinded,but the outcome evalu-ators and data analysts were blinded.One-hundred-and-two patients with stable COPD were randomly divided into two groups in a 1∶1 ratio.The acupuncture group received 30-minute sessions of acupuncture therapy at the same acupoints three times weekly for 6 weeks in addition to routine conventional Western Medicine treatment;the control group received routine conventional Western Medicine treatment alone.The primary outcome was the Borg scale score,which was assessed immediately after the 6-minute walk test.The secondary outcomes were the 6-minute walk distance,lung function,and oxygen saturation.Measurements were obtained at baseline and after 6 weeks of treatment.RESULTS:After 6 weeks of treatment,the Borg scale score in the acupuncture group was significantly better than that in the control group(2.02±0.71 versus 5.01±0.34,P<0.05).Furthermore,the post-treatment improvements in the 6-minute walk distance,lung function,and oxygen saturation were significantly greater in the acupuncture group than in the control group,showing that the acupuncture group had better exercise tolerance.CONCLUSION:The findings suggest that acupuncture that aims to reinforce the spleen to strengthen the lung is a safe and effective adjuvant therapy that effectively improves the exercise capacity of patients with stable COPD. | Li Yu Xiong Chan Zeng Yan Wei Hua Zhuang Guangtong Zhao Lihong Li Chenyi Li Lin Qin Erqi Chen Xiaoqin Fu Juanjuan | 2019 | Journal of Traditional Chinese Medicine2019，39，6： | 13 |
| 16 | Expression and alterations of different molecular form γ-glutamyl transferase and total RNA concentration during the carcinogenesis of rat hepatoma显示文摘INTRODUCTIONCarcinogenesisiscloselyrelatedwithDNAsynthesisandhypermetabolismofnucleicacid.γglutamyltransferase(GGT,EC2.3.2.2... | TANG Qi Yun, YAO Deng Fu, LU Jian Xin, WU Xin Hua and MENG Xian Yong | 1999 | World Journal of Gastroenterology1999，5，4： | 12 |
| 17 | H5N1 influenza virus-specific miRNA-like small RNA increases cytokine production and mouse mortality via targeting poly(rC)-binding protein 2显示文摘H5N1 流行性感冒病毒的感染在所有流行性感冒病毒之中引起最高的死亡。位于如此的高病毒的致病力下面的机制不完全地被理解。这里,我们报导 H5N1 流行性感冒病毒编码象 microRNA 一样小 RNA, miR-HA-3p,它从茎被处理由 Argonaute 2 的包含环的病毒的 RNA 先锋,和戏在在 H5N1 感染期间提高 cytokine 生产的一个角色。miR-HA-3p poly 指向的机械学的学习表演(rC ) 有约束力的蛋白质 2 (PCBP2 ) 并且压制它的表达式。与 PCBP2 being 一致 RIG-I/MAVS-mediated 抗病毒的天生的免疫的一个重要否定管理者,由 miR-HA-3p 的 PCBP2 表示的抑制在感染 H5N1 的人的巨噬细胞和老鼠支持 cytokine 生产病毒。我们断定 miR-HA-3p 是首先识别的流行性感冒编码病毒的象 microRNA 一样功能的 RNA 碎片和贡献导致 H5N1 的 cytokine 暴风雨和死亡的一个新奇毒力因素。 | Xihan Li Zheng Fu Hongwei Liang Yanbo Wang Xian Qi Meng Ding Xinlei Sun Zhen Zhou Ying Huang Hongwei Gu Limin Li Xi Chen Donghai Li Quan Zhao Fenyong Li Hua Wang Jin Wang Ke Zen Chen-Yu Zhang | 2018 | Cell Research2018，28，2： | 12 |
| 18 | Identification and Fine Mapping of a Gene Related to Pale Green Leaf Phenotype near the Centromere Region in Rice(Oryza sativa)显示文摘A thermo-insensitive pale green leaf mutant (pgl2) was isolated from T-DNA inserted transgenic lines of rice (Oryza sativa L. subsp. japonica cv. Nipponbare). Genetic analysis indicated that the phenotype was caused by a recessive mutation in a single nuclear-encoded gene. To map the PGL2 gene, an F2 population was constructed by crossing the mutant with Longtefu (Oryza sativa L. subsp. indica). The PGL2 locus was roughly linked to SSR marker RM331 on chromosome 8. To finely map the gene, 14 new InDel markers were developed around the marker, and PGL2 was further mapped to a 2.37 Mb centromeric region. Analysis on chlorophyll contents of leaves showed that there was no obvious difference between the mutant and the wild type in total chlorophyll (Chl) content, while the ratio of Chl a / Chl b in the mutant was only about 1, which was distinctly lower than that in the wild type, suggesting that the PGL2 gene was related to the conversion between Chl a and Chl b. Moreover, the method of primer design around the centromeric region was discussed, which would provide insight into fine mapping of the functional genes in plant centromeres. | ZHU Li LiuWenzhen WU Chao LUAN Wei jiang Fu Ya ping Hu Guo cheng SI Hua min SUN Zong xiu | 2007 | Rice science2007，14，3： | 11 |
| 19 | Sequencing of p53 mutation in established human hepatocellular carcinoma cell line of HHC4 and HHC15 in nude mice显示文摘INTRODUCTIONAlthoughtheetiopathologyofhumanhepatocelularcarcinoma(hHCC)isstilunknown,alotofevidencestronglysuggestedthatinfec... | YANG Shan Min, ZHOU Hong, CHEN Rui Chuan, WANG Yu Fang, CHEN Fu, ZHANG Chang Gong, ZHEN Yun, YAN Jiang Hua and SU Jin Hua | 1998 | World Journal of Gastroenterology1998，4，6： | 11 |
| 20 | Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)-associated endonuclease 9(CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T_0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T_0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T_0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding. | Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie Wang Xingchun Wang Changjie Yan Kejian Wang | 2017 | Science China(Life Sciences)2017，60，5： | 11 |
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