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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | Sofosbuvir and ledipasvir fixed-dose combination with and without ribavirin in treatment-naive and previously treated patients with genotype 1 hepatitis C virus infection (LONESTAR): an open-label, randomised, phase 2 trial显示文摘 | Eric Lawitz Fred F Poordad Phillip S Pang Robert H Hyland Xiao Ding Hongmei Mo William T Symonds John G McHutchison Fernando E Membreno | 2013 | The Lancet2013，，： | 5 |
| 3 | Concurrent effects of high hydrostatic pressure, acidity and hear on destruction an injury yeast 显示文摘 | PandeyaYoga Jewett Fred F Hoover | 1995 | J of Food Protection1995，58，： | 1 |
| 4 | Powder processing science and technology for increased reliability显示文摘 | | 1989 | J Am Ceram Soc1989，72，1： | 1 |
| 5 | Comparison of airborne hyperspectral data and EO-1 hyperion for mineral mapping 显示文摘 | Fred A Kruse Joseph W Boardman Jonathan F Huntington | 2003 | IEEE2003，41，6： | 1 |
| 6 | Genetic diversity of Plasmodium falciparum infections in mild and severe malaria of children from Kampala, Uganda显示文摘 | Mpungu Steven Kiwuwa Ulf Ribacke Kirsten Moll Justus Byarugaba Klara Lundblom Anna F?rnert Kironde Fred Mats Wahlgren | 2013 | Parasitology Research2013，，4： | 1 |
| 7 | Mechanical properties of the posterolateral structures of the knee显示文摘 | Robert F Laprade Timothy S Bollom Fred A Wentorf | | 0，，： | 1 |
| 8 | Stress Triggering of the 1999 Hector Mine Earthquake by Transient Deformation Following the 1992 Landers Earthquake显示文摘 | Fred F Pollitz I Selwyn Sacks | 2000 | Bulletin of the Seismological Society of America2000，92，4： | 1 |
| 9 | Catalysis of the electroreduction of O2 by cobalt 5,10,15,20-tetraPhenylPorphyrin dissolved in thin layers of benzonitrile on graphite electrodes显示文摘 | CHUNG T D FRED C ANSON F C | | 0，，01： | 1 |
| 10 | Challenges for fuel cells in transport applications显示文摘 | Chalk S G Miller J F Fred W J | 2000 | Power Source2000，86，： | 1 |
| 11 | Astroglia induce neurogenesis from adult neural stem cells显示文摘 | Hongjun Song Charles F Stevens Fred H Gage | 2002 | Nature2002，417，： | 1 |
| 12 | Safer sex communication and unsafe sexual behavior among young men who have sex with men in Calirofnial显示文摘 | Fred M Matthew F Juan DR | 1999 | Archives of Sexual Behavior1999，28，4： | 1 |
| 13 | Radiofrequency catheter ablation of inappropriate sinus tachycardia guided by activation mapping显示文摘 | K.Ching Man Bradley Knight Hung-Fat Tse Frank Pelosi Gregory F Michaud Matthew Flemming S.Adam Strickberger Fred Morady | 2000 | Journal of the American College of Cardiology2000，，2： | 1 |
| 14 | Stress- induced shifts of first-order raman frequencies ofdiamond and zinc-blend-type semiconductors 显示文摘 | Cerdeira F Buchenauer C J Poliak Fred H | 1972 | Physical Review B1972，5，2： | 1 |
| 15 | Histopathological effects and growth reduction in a susceptible and a resistance of heliothis virescens caused by sublethal doses of pure Cryl A crystal proteins from Bacillus thuringiesis 显示文摘 | Amparoc M Fred G Juan F | 1999 | Biocontrol Science and Technology1999，9，2： | 1 |
| 16 | Thermosetting acetylene-terminated polyphenyiquinoxalines显示文摘 | Robert F Kovar Gerhard F L Ehlers Fred E Arnold | 1977 | Journal of Polymer Science: Polymer Chemistry Edition1977，15，5： | 1 |
| 17 | Life History Traits of the Tennessee Dace (Phoxinus tennesseensis) in Northeast Tennessee显示文摘 | Hamed M Kevin Alsop Fred J Laughlin Thomas F | 2008 | The American Midland Naturalist2008，，2： | 1 |
| 18 | Influence of inorganic addi- tives on oxygen chemisorption on cellulosic chars 显示文摘 | William F Degroot Fred Shafizadeh | 1983 | Carbon1983，21，1： | 1 |
| 19 | Micro-offsets and macro-transformation:an inconvenient view of climate change justice显示文摘 | Michael P V Brooke A A Fred E F | 2009 | Harvard Environmental Law Review2009，，33： | 1 |
| 20 | Interfacial electrochemistry of cytochrome c at tin oxide,indium oxide,gold,and platinum electrodes显示文摘 | EDMOND F B FRED M H HENRY N B | 1984 | J Electroanal Chem1984，161，： | 1 |
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