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| 1 | Targeting Wnt signaling at the neuroimmune interface for dopaminergic neuroprotectionJrepair in Parkinson's disease显示文摘 | Francesca L'Episcopo Cataldo Tirolo Salvo Caniglia Nuccio Testa Maria Concetta Moral~ Maria Francesca Serapide: Stefano Pluchino Bianca Marchetti | 2014 | Journal of Molecular Cell Biology2014，8，1： | 8 |
| 2 | Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection显示文摘Hepatitis delta virus(HDV) seems to strongly suppress hepatitis B virus(HBV)replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein(HBx) is essential for HBV replication, determination of HBV X gene(HBX)quasispecies complexity in HBV/HDV infection compared to HBV monoinfection may provide information on the interactions between these two viruses.AIM To compare HBV quasispecies complexity in the HBX 5' region between chronic hepatitis delta(CHD) and chronic HBV mono-infected patients.METHODS Twenty-four untreated patients were included: 7/24(29.2%) with HBeAgnegative chronic HBV infection(CI, previously termed inactive carriers), 8/24(33.3%) with HBeAg-negative chronic hepatitis B(CHB) and 9/24(37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels.The HBX 5' region [nucleotides(nt) 1255-1611] was then PCR-amplified for subsequent next-generation sequencing(MiSeq, Illumina, United States). HBV quasispecies complexity in the region analyzed was evaluated using incidencebased indices(number of haplotypes and number of mutations), abundancebased indices(Hill numbers of order 1 and 2), and functional indices(mutation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them could be the cause of the quasispecies complexity.RESULTS CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL,interquartile range(IQR) 3.5-7.9] than CHD(3.4 logIU/mL, IQR 3-7.6)(P = n.s.)or CI(3.2 logIU/mL, IQR 2.3-3.5)(P < 0.01) patients. The incidence and abundance indices indicated that HBV quasispecies complexity was significantly greater in CI than CHB. A similar trend was observed in CHD patients, although only Hill numbers of order 2 showed statistically significant differences(CHB2.81, IQR 1.11-4.57 vs CHD 8.87, 6.56-11.18, P = 0.038). There were no significant differences in the functional indices, but CI and CHD patients also showed a trend towards greater complexity than CHB. No differences were found for any HBV quasispecies complexity indices between CHD and CI patients. G-to-A and C-to-T nucleotide changes, characteristic of APOBEC3 G, were higher in CHD and CI than in CHB in genotype A haplotypes, but not in genotype D. The proportion of nt G-to-A vs A-to-G changes and C-to-T vs T-to-C changes in genotype A and D haplotypes in CHD patients showed no significant differences. In CHB and CI the results of these comparisons were dependent on HBV genotype.CONCLUSION The lower-replication CHD and CI groups show a trend to higher quasispecies complexity than the higher-replication CHB group. The mechanisms associated with this greater complexity require elucidation. | Cristina Godoy David Tabernero Sara Sopena Josep Gregori Maria Francesca Cortese Carolina González Rosario Casillas Mar?al Yll Ariadna Rando Rosa López-Martínez Josep Quer Gloria González-Aseguinolaza Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías | 2019 | World Journal of Gastroenterology2019，25，13： | 6 |
| 3 | Analysis of hepatitis B virus preS1 variability and prevalence of the rs2296651 polymorphism in a Spanish population显示文摘AIM To determine the variability/conservation of the domain of hepatitis B virus(HBV) pre S1 region that interacts with sodium-taurocholate cotransporting polypeptide(hereafter, NTCP-interacting domain) and the prevalence of the rs2296651 polymorphism(S267 F, NTCP variant) in a Spanish population. METHODS Serum samples from 246 individuals were included and divided into 3 groups: patients with chronic HBV infection(CHB)(n = 41, 73% Caucasians), patients with resolved HBV infection(n = 100, 100% Caucasians) and an HBV-uninfected control group(n = 105, 100% Caucasians). Variability/conservation of the amino acid(aa) sequences of the NTCPinteracting domain,(aa 2-48 in viral genotype D) and a highly conserved pre S1 domain associated with virion morphogenesis(aa 92-103 in viral genotype D) were analyzed by next-generation sequencing and compared in 18 CHB patients with viremia > 4 log IU/mL. The rs2296651 polymorphism was determined in all individuals in all 3 groups using an in-house real-time PCR melting curve analysis.RESULTS The HBV pre S1 NTCP-interacting domain showed a high degree of conservation among the examined viral genomes especially between aa 9 and 21(in the genotype D consensus sequence). As compared with the virion morphogenesis domain, the NTCPinteracting domain had a smaller proportion of HBV genotype-unrelated changes comprising > 1% of the quasispecies(25.5% vs 31.8%), but a larger proportion of genotype-associated viral polymorphisms(34% vs 27.3%), according to consensus sequences from Gen Bank patterns of HBV genotypes A to H. Variation/conservation in both domains depended on viral genotype, with genotype C being the most highly conserved and genotype E the most variable(limited finding, only 2 genotype E included). Of note, proline residues were highly conserved in both domains, and serine residues showed changes only to threonine or tyrosine in the virion morphogenesis domain. The rs2296651 polymorphism was not detected in any participant.CONCLUSION In our CHB population, the NTCP-interacting domain was highly conserved, particularly the proline residues and essential amino acids related with the NTCP interaction, and the prevalence of rs2296651 was low/null. | Rosario Casillas David Tabernero Josep Gregori Irene Belmonte Maria Francesca Cortese Carolina González Mar Riveiro-Barciela Rosa Maria López Josep Quer Rafael Esteban Maria Buti Francisco Rodríguez-Frías | 2018 | World Journal of Gastroenterology2018，24，6： | 5 |
| 4 | Comprehensive review of autoantibodies in patients with hyper-IgM syndrome显示文摘Hyper-immunoglobulin M syndrome is an X-linked primary immunodeficiency disease caused by mutations in the CD40 ligand gene.The CD40 ligand has been recently highlighted as playing a key role in the pathogenesis of primary biliary cholangitis.In the present study,we assessed an extensive set of serum autoantibodies in a series of well-defined patients with hyper-immunoglobulin M syndrome.Serum,liver-related and liver-not-related autoantibodies IgG,IgM and IgA were tested by ELISA and standard indirect immunofluorescence in HEp-2 cells in 13 Tunisian patients(8 males and 5 females,aged 1–12 years)with hyper-immunoglobulin M syndrome during 1995–2012 and,as controls,21 age-and gender-matched blood donors.The level of IgM antibody against MIT3 was significantly higher in patients than in controls(35.8 vs 10.7,P=0.002).Half of the hyperimmunoglobulin M syndrome patients were found to be anti-MIT3 IgM positive vs none of the controls(Po0.0001).Twenty-three percent of patients were found to be anti-sp100 antibody positive vs only 0.05%of controls.By immunofluorescence,92.3%of patients were MIT3 IgM positive vs none of the controls.In conclusion,the IgM class of anti-MIT3 antibodies was shown to be present by both ELISA and immunofluorescence in most of the patients with hyper-immunoglobulin M syndrome.The presence of the hallmark of primary biliary cholangitis,a disease where the CD40 ligand is a key player,in an immunodeficiency disease caused by mutations in the CD40 ligand gene is very intriguing and opens new scenarios in understanding the immune pathogenesis of primary biliary cholangitis. | Mohamed-Ridha Barbouche Qubo Chen Marco Carbone Imen Ben-Mustapha Zakera Shums Mehdi Trifa Federica Malinverno Francesca Bernuzzi Haiyan Zhang Nourhen Agrebi Gary L Norman Christopher Chang M Eric Gershwin Pietro Invernizzi | 2018 | Cellular & Molecular Immunology2018，15，6： | 2 |
| 5 | Dimensions of health related dispositions of elderly people : relationships with heahh behavior and personality traits 显示文摘 | Artistico D Francesca B Marco L L | 2000 | European Journal of personality2000，，14： | 1 |
| 6 | Disulfiram-induced cytotoxicity and endo-lysosomal sequestration of zinc in breast cancer cells显示文摘 | HELEN L W JENNIFER M W FRANCESCA S | 2015 | Journal of Biochemical Pharmacology2015，，93： | 1 |
| 7 | New protocol for construction of eyeglasses-supported provisional nasal prosthesis using CAD/CAM techniques显示文摘 | Ciocca L Massimiliano F Francesca DC | 2010 | J Rehabil Res Dev2010，47，7： | 1 |
| 8 | Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering显示文摘 | Michel B Francesca B Massimilliano L | 2007 | Biomolecular Engineering2007，24，6： | 1 |
| 9 | First in class, potent, and orally bioavailable NADPH oxidase isoform 4 (Noxg) inhibitors for the treatment of idiopathic pulmonary fibro- sis显示文摘 | BENOIT L FRANCESCA G ORCHARD M | 2010 | J Med Chem2010，53，21： | 1 |
| 10 | Molecu-lar basis of funny current(If)in normal and failing humanheart显示文摘 | FRANCESCA S GIUSEPPE L STEPHEN Z | 2008 | J Mol Cell Cardiol2008，45，2： | 1 |
| 11 | Interfacial dilational rheology by oscillating bubble/drop methods显示文摘 | FRANCESCA R GIUSEPPE L VOLODYMYR I K | 2010 | Current Opinion in Colloid & Interface Science2010，15，： | 1 |
| 12 | Longer dialysis session length is associated with better intermediate outcomes and survival among patients on in-center three times per week hemodialysis: results from the Dialysis Outcomes and Practice Patterns Study (DOPPS) 显示文摘 | Francesca T Jinyao Z Yun L | 2012 | Nephrol Dial Transplant2012，27，11： | 1 |
| 13 | The Retrogradation of Concentrated Wheat Starch Systems显示文摘 | Francesca L | 2005 | Starch2005，57，： | 1 |
| 14 | Detection of genetically modified soybean using peptide nucleic acids (PNAs)and microarray technology显示文摘 | Andrea G Alessandra M Francesca L | 2005 | Journal of Agricultural and Food Chemistry2005，52，14： | 1 |
| 15 | Polynomial chaos repre- sentation for identification of mechanical characteristics ofinstrumented structures 显示文摘 | Franck S Humberto Y Francesca L | 2011 | Computer-Aided Civil and Infras- tructure Engineering2011，26，3： | 1 |
| 16 | Yeast ecolo- gy of vineyards within Marsala wine area (western Sicily) in two consecutive vintages and selection of autochthonous Saccharomyces cerevisiae strains 显示文摘 | Settanni L Sannino C Francesca N | 2012 | Journal of Biosci- ence and Bioengineering2012，114，6： | 1 |
| 17 | Selective trace enrichment of chlorotriazine pesticides from natural waters and sediment samples using terbuthylazine molecularly imprinted polymers显示文摘 | IMMA F FRANCESCA L ANTAL T | 2000 | Anal Chem2000，72，： | 1 |
| 18 | Increased risk for endocrine autoimmunity in Italian type 2 diabetic pa- tients with GAD65 antoantibodies显示文摘 | Giovanni G Francesca F Stefano L | 2000 | Clinical Endocri- nology2000，52，9： | 1 |
| 19 | The prosnostic significance of micrometastases in breast cancer:A SEER population-based analysis显示文摘 | Steven L Chen Francesca M Hoehne Armando E Giuliano | 2007 | Ann Surg Oncol2007，12，14： | 1 |
| 20 | Systemic exposure to paraquat and maneb models early Parkinson's disease in young adult rats显示文摘 | Francesca C Nicolas L Antoine RT | 2005 | Neurobiol Dis2005，20，2： | 1 |
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