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| 1 | Liberation of SARS-CoV main protease from the viral polyprotein:N-terminal autocleavage does not depend on the mature dimerization mode显示文摘The main protease(M^(pro))plays a vital role in proteolytic processing of the polyproteins in the replicative cycle of SARS coronavirus(SARS-CoV).Dimerization of this enzyme has been shown to be indispensable for transcleavage activity.However,the auto-processing mechanism of M^(pro),i.e.its own release from the polyproteins through autocleavage,remains unclear.This study elucidates the relationship between the N-terminal autocleavage activity and the dimerization of“immature”M^(pro).Three residues(Arg4,Glu290,and Arg298),which contribute to the active dimer conformation of mature M^(pro),are selected for mutational analyses.Surprisingly,all three mutants still perform N-terminal autocleavage,while the dimerization of mature protease and transcleavage activity following auto-processing are completely inhibited by the E290R and R298E mutations and partially so by the R4E mutation.Furthermore,the mature E290R mutant can resume N-terminal autocleavage activity when mixed with the“immature”C145A/E290R double mutant whereas its trans-cleavage activity remains absent.Therefore,the N-terminal auto-processing of M^(pro) appears to require only two“immature”monomers approaching one another to form an“intermediate”dimer structure and does not strictly depend on the active dimer conformation existing in mature protease.In conclusion,an auto-release model of M^(pro) from the polyproteins is proposed,which will help understand the auto-processing mechanism and the difference between the autocleavage and trans-cleavage proteolytic activities of SARS-CoV M^(pro). | Shuai Chen Felix Jonas Can Shen Rolf Higenfeld | 2010 | Protein & Cell2010，1，1： | 3 |
| 2 | Gastric Bypass Leads to Improvement of Diabetic Neuropathy Independent of Glucose Normalization—Results of a Prospective Cohort Study (DiaSurg 1 Study)显示文摘 | Beat P. Müller-Stich Lars Fischer Hannes G. Kenngott Matthias Gondan Jonas Senft Gabriella Clemens Felix Nickel Thomas Fleming Peter P. Nawroth Markus W. Büchler | 2013 | Annals of Surgery2013，，5： | 2 |
| 3 | Serotonin type 3 receptor subunit gene polymorphisms associated with psychosomatic symptoms in irritable bowel syndrome:A multicenter retrospective study显示文摘BACKGROUND Single-nucleotide polymorphisms(SNPs)of the serotonin type 3 receptor subunit(HTR3)genes have been associated with psychosomatic symptoms,but it is not clear whether these associations exist in irritable bowel syndrome(IBS).AIM To assess the association of HTR3 polymorphisms with depressive,anxiety,and somatization symptoms in individuals with IBS.METHODS In this retrospective study,623 participants with IBS were recruited from five specialty centers in Germany,Sweden,the United States,the United Kingdom,and Ireland.Depressive,anxiety,and somatization symptoms and sociodemographic characteristics were collected.Four functional SNPs—HTR3A c.-42C>T,HTR3B c.386A>C,HTR3C c.489C>A,and HTR3E c.*76G>A—were genotyped and analyzed using the dominant and recessive models.We also performed separate analyses for sex and IBS subtypes.SNP scores were calculated as the number of minor alleles of the SNPs above.The impact of HTR3C c.489C>A was tested by radioligand-binding and calcium influx assays.RESULTS Depressive and anxiety symptoms significantly worsened with increasing numbers of minor HTR3C c.489C>A alleles in the dominant model(F_(depressive)=7.475,P_(depressive)=0.006;F_(anxiety)=6.535,P_(anxiety)=0.011).A higher SNP score(range 0-6)was linked to a worsened depressive symptoms score(F=7.710,P-linear trend=0.006)in IBS.The potential relevance of the HTR3C SNP was corroborated,showing changes in the expression level of 5-HT3AC variant receptors.CONCLUSION We have provided the first evidence that HTR3C c.489C>A is involved in depressive and anxiety symptoms in individuals with IBS.The SNP score indicated that an increasing number of minor alleles is linked to the worsening of depressive symptoms in IBS. | Sabrina Berens Yuanjun Dong Nikola Fritz Jutta Walstab Mauro D'Amato Tenghao Zheng Verena Wahl Felix Boekstegers Justo Lorenzo Bermejo Cristina Martinez Stefanie Schmitteckert Egbert Clevers Felicitas Engel Annika Gauss Wolfgang Herzog Robin Spiller Miriam Goebel-Stengel Hubert Mönnikes Viola Andresen Frieling Thomas Jutta Keller Christian Pehl Christoph Stein-Thöringer Gerard Clarke Timothy G Dinan Eamonn M Quigley Gregory Sayuk Magnus Simrén Jonas Tesarz Gudrun Rappold Lukas van Oudenhove Rainer Schaefert Beate Niesler | 2022 | World Journal of Gastroenterology2022，28，21： | 2 |
| 4 | Quantitation andidentity confirmation of residues of quinolones in tilapia fillets by LC-ESI-MS-MS QToF显示文摘 | JONAS A R P FELIX G R R SUSANNE R | 2009 | Anal Bioanal Chem2009，，： | 1 |
| 5 | Simulation of cell stacks using a computer-controlled power rectifier with the purposes of actual high-power injection applications显示文摘 | Jeferson M C Felix A F Jonas R Gomes | 2003 | IEEE Trans on Industry Application2003，39，4： | 1 |
| 6 | Artificial intelligence in pancreatic surgery:current applications显示文摘Surgery plays a central role in the treatment of benign and malignant pancreatic diseases.Artificial intelligence(AI)is an important upcoming technology to support surgeons in pre-,intra-,and postoperative diagnosis,decision-making and training toward an optimized patient care.Current AI applications show a promising role in the evaluation of preoperative images for prediction of malignancy and resectability,intraoperative decision support,surgical training as well as a postoperative risk stratification to per-sonalize the management of complications.This scoping review summarizes the most up to date developments of AI in pancreatic surgery with the highest available level of evidence. | Christoph Kuemmerli Fabian Rössler Caroline Berchtold Michael C.Frey Alexander Studier-Fischer Amila Cizmic Jan Philipp Jonas Thilo Hackert Felix Nickel Philip C.Müller | 2023 | Journal of Pancreatology2023，6，2： | 0 |
| 7 | Liberation of SARS-CoV main protease from the viral polyprotein:N-terminal autocleavage does not depend on the mature dimerization mode显示文摘 | Shuai Chen Felix Jonas Can Shen Rolf Hilgenfeld | 2010 | Protein & Cell2010，1，3： | 0 |
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