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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system显示文摘 | Haifeng Wan Chunjing Feng Fei Teng Shihua Yang Baoyang Hu Yuyu Niu Andy Peng Xiang Weizhen Fang Weizhi Ji Wei Li Xiaoyang Zhao Qi Zhou | 2015 | Cell Research2015，25，2： | 39 |
| 3 | Prevalence of HPV Infection And Cervical Intraepithelial Neoplasia And Attitudes towards HPV Vaccination among Chinese Women Aged 18-25 in Jiangsu Province显示文摘Objective:Few data are available on the epidemiology of HPV and cervical cancer among Chinese women younger than 25 years old.This study aimed to estimate the HPV infection rate and the prevalence of cervical intraepithelial neoplasia(CIN) in women aged 18-25,as well as their knowledge of and attitudes towards HPV vaccination.Methods:A population-based cervical cancer screening study was conducted on women aged 18-25 in Jiangsu province in 2008.Participants provided socio-demographic,reproductive and behavioral information and completed a survey about their knowledge of and attitudes towards HPV vaccination.Women then underwent a gynecologic exam to provide two cervical exfoliated cell samples for high risk HPV DNA testing and liquid-based cytology(LBC) as well as visual inspection with acetic acid(VIA).Women testing positive for any test were referred to colposcopy and biopsy.The gold standard for diagnosis of cervical lesions was directed or random biopsies.Results:Within the sample of 316 women,3.4% of them were diagnosed with CIN grade 2 or worse lesions and 17.1% were found to be positive for HPV DNA.Among these young women,extra-marital sexual behavior of them(OR=2.0,95%CI:1.1-3.8) or their husbands(OR=2.6,95%:1.4-4.7) were associated with an increased risk of HPV positivity.Although overall HPV awareness was low,after a brief educational intervention,98.4% reported they would electively receive HPV vaccination and would also recommend that their daughters be vaccinated.However,most urban and rural women reported their ideal maximum out-of-pocket contribution for HPV vaccination to be less than 500 RMB and 50-100 RMB,respectively.Conclusion:Our study indicates cervical disease burden is relatively high among sampled Chinese women aged 18-25.Appropriate educational interventions for female adolescents and strategies to subsidize vaccine costs are definitely needed to ensure the effectiveness of vaccination campaigns in China. | Shang-ying Hu Ying Hong Fang-hui Zhao Adam K. Lewkowitz Feng Chen Wen-hua Zhang O.in-jing Pan Xun Zhang Cindy Fei Hui Li You-lin Qiao | 2011 | Chinese Journal of Cancer Research2011，23，1： | 31 |
| 4 | Advanced treatment of dyeing wastewater towards reuse by the combined Fenton oxidation and membrane bioreactor process显示文摘The performance of combined Fenton oxidation and membrane bioreactor (MBR) process for the advanced treatment of an effluent from an integrated dyeing wastewater treatment plant was evaluated. The experimental results revealed that under the optimum Fenton oxidation conditions (initial pH 5, H 2 O 2 dosage 17 mmol/L, and Fe 2+ 1.7 mmol/L) the average total organic carbon (TOC) and color removal ratios were 39.3% and 69.5% after 35 min of reaction, respectively. Results from Zahn-Wallens Test also represented that Fenton process was effective to enhance the biodegradability of the test wastewater. As for the further purification of MBR process, TOC removal capacity was examined at different hydraulic retention times (HRT) of 10, 18 and 25 hr. Under the optimum HRT of 18 hr, the average TOC concentration and color of the final MBR effluent were 16.8 mg/L and 2 dilution time, respectively. The sludge yield coefficient was 0.13 g MLSS/g TOC and TOC degradation rate was 0.078 kg TOC/(m 3 ·day). The final effluent of MBR can meet the reuse criteria of urban recycling water-water quality standard for miscellaneous water consumption GBT18920-2002. | Fei Feng Zhenliang XU Xiaohuan Li Wenting You Yang Zhen | 2010 | Journal of Environmental Sciences2010，22，11： | 27 |
| 5 | Pathways of Influence of the Northern Hemisphere Mid–high Latitudes on East Asian Climate: A Review显示文摘This paper reviews recent progress made by Chinese scientists on the pathways of influence of the Northern Hemisphere mid-high latitudes on East Asian climate within the framework of a“coupled oceanic-atmospheric(land-atmospheric or seaice-atmospheric)bridge”and“chain coupled bridge”.Four major categories of pathways are concentrated upon,as follows:Pathway A—from North Atlantic to East Asia;Pathway B—from the North Pacific to East Asia;Pathway C—from the Arctic to East Asia;and Pathway D—the synergistic effects of the mid-high latitudes and tropics.In addition,definitions of the terms“combined effect”,“synergistic effect”and“antagonistic effect”of two or more factors of influence or processes and their criteria are introduced,so as to objectively investigate those effects in future research. | Jianping LI Fei ZHENG Cheng SUN Juan FENG Jing WANG | 2019 | Advances in Atmospheric Sciences2019，36，9： | 27 |
| 6 | Hepatitis C virus infection of human hepatoma cell line 7721 in vitro显示文摘AIM To establish a cell culture system with long-term replication of hepatitis C virus in vitro.``METHODS Human hepatoma cell line 7721 was tested for its susceptibility to HCV by incubating with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various phases during the culturing periods The presence of HCV RNA, the expression of HCV antigens in cells and/or supernatant were examined by RT-PCR, in situ hybridization and immunohistochemistry respectively.``RESULTS The intracellular HCV RNA was first detected on d 2 after infection and then could be intermittently detected in both cells and supernatant over a period of at least three months. The expression of HCV NS3, CP10antigens could be observed in cells. The fresh cells could be infected by supematant from cultured infected cells and the transmission of viral genome from HCV-infected 7721 cells to PBMCs was also observed.``CONCLUSION The hepatoma line 7721 is not only susceptible to HCV but also supports its long-term replication in vitro. | Zhi-Qiang Song~1 Fei Hao~1 Feng Min~2 Qiao-Yu Ma~2 Guo-Dong Liu~2 Department of Dermatology~1Department of Infectious Diseases~2,Southwest Hospital,Third Military Medical University,Chongqing 400038,China | 2001 | World Journal of Gastroenterology2001，7，5： | 26 |
| 7 | VGLL4 functions as a new tumor suppressor in lung cancer by negatively regulating the YAP-TEAD transcriptional complex显示文摘肺癌症是与高发生和死亡世界范围的大多数破坏疾病之一。河马(Hpo ) 小径是在果蝇和哺乳动物的机关尺寸的一个保存管理者。新兴的证据在癌症开发建议了 Hpo 小径的意义。在这研究,我们通过否定地调整 YAP-TEAD 建筑群的形成在肺 carcinogenesis 作为新奇肿瘤 suppressor 识别 VGLL4, Hpo 的核心部件小径。我们 VGLL4 经常被观察低层的数据表演在老鼠和人的肺癌症标本表示了。VGLL4 的宫外的表示显著地在 vitro 压制肺癌症房间的生长。更重要地, VGLL4 显著地在 de novo 老鼠模型禁止肺癌症前进。我们进一步发现 VGLL4 禁止 YAP-TEAD transcriptional 建筑群的活动。我们 VGLL4 直接在绑定与笨蛋竞争到 TEAD 并且执行的数据表演它通过二 TDU 域的生长禁止的函数。一起,我们的学习证明 VGLL4 是为通过否定地调整 YAP-TEAD 复杂形成并且这样的肺癌症的新奇肿瘤 suppressor Hpo 小径。 | Wenjing Zhang Yijun Gao Peixue Li Zhubing Shi Tong Guo Fei Li Xiangkun Han Yan Feng Chao Zheng Zuoyun Wang Fuming Li Haiquan Chen Zhaocai Zhou Lei Zhang Hongbin Ji | 2014 | Cell Research2014，24，3： | 25 |
| 8 | Minimal invasive microscopic tooth preparation in esthetic restoration: a specialist consensus显示文摘By removing a part of the structure,the tooth preparation provides restorative space,bonding surface,and finish line for various restorations on abutment.Preparation technique plays critical role in achieving the optimal result of tooth preparation.With successful application of microscope in endodontics for>30 years,there is a full expectation of microscopic dentistry.However,as relatively little progress has been made in the application of microscopic dentistry in prosthodontics,the following assumptions have been proposed:Is it suitable to choose the tooth preparation technique under the naked eye in the microscopic vision?Is there a more accurate preparation technology intended for the microscope?To obtain long-term stable therapeutic effects,is it much easier to achieve maximum tooth preservation and retinal protection and maintain periodontal tissue and oral function health under microscopic vision?Whether the microscopic prosthodontics is a gimmick or a breakthrough in obtaining an ideal tooth preparation should be resolved in microscopic tooth preparation.This article attempts to illustrate the concept,core elements,and indications of microscopic minimally invasive tooth preparation,physiological basis of dental pulp,periodontium and functions involved in tool preparation,position ergonomics and visual basis for dentists,comparison of tooth preparation by naked eyes and a microscope,and comparison of different designs of microscopic minimally invasive tooth preparation techniques.Furthermore,a clinical protocol for microscopic minimally invasive tooth preparation based on target restorative space guide plate has been put forward and new insights on the quantity and shape of microscopic minimally invasive tooth preparation has been provided. | Haiyang Yu Yuwei Zhao Junying Li Tian Luo Jing Gao Hongchen Liu Weicai Liu Feng Liu Ke Zhao Fei Liu Chufan Ma Juergen MSetz Shanshan Liang Lin Fan Shanshan Gao Zhuoli Zhu Jiefei Shen Jian Wang Zhimin Zhu Xuedong Zhou | 2019 | International Journal of Oral Science2019，11，4： | 25 |
| 9 | Size distribution of chemical elements and their source apportionment in ambient coarse, fine, and ultrafine particles in Shanghai urban summer atmosphere显示文摘Ambient coarse particles (diameter 1.8-10 μm), fine particles (diameter 0.1-1.8 μm), and ultrafine particles (diameter < 0.1 μm) in the atmosphere of the city of Shanghai were sampled during the summer of 2008 (from Aug 27 to Sep 08). Microscopic characterization of the particles was investigated by scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDX). Mass concentrations of Si, P, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Br, Rb, Sr, and Pb in the size-resolved particles were quantified by using synchrotron radiation X-ray fluorescence (SRXRF). Source apportionment of the chemical elements was analyzed by means of an enrichment factor method. Our results showed that the average mass concentrations of coarse particles, fine particles and ultrafine particles in the summer air were 9.38 ± 2.18, 8.82 ± 3.52, and 2.02 ± 0.41 μg/m3, respectively. The mass percentage of the fine particles accounted for 51.47% in the total mass of PM10, indicating that fine particles are the major component in the Shanghai ambient particles. SEM/EDX results showed that the coarse particles were dominated by minerals, fine particles by soot aggregates and fly ashes, and ultrafine particles by soot particles and unidentified particles. SRXRF results demonstrated that crustal elements were mainly distributed in the coarse particles, while heavy metals were in higher proportions in the fine particles. Source apportionment revealed that Si, K, Ca, Fe, Mn, Rb, and Sr were from crustal sources, and S, Cl, Cu, Zn, As, Se, Br, and Pb from anthropogenic sources. Levels of P, V, Cr, and Ni in particles might be contributed from multi-sources, and need further investigation. | Senlin Lu Rui Zhang Zhenkun Yao Fei Yi Jingjing Ren Minghong Wu Man Feng Qingyue Wang | 2012 | Journal of Environmental Sciences2012，24，5： | 22 |
| 10 | The Chinese Society of Clinical Oncology(CSCO)clinical guidelines for the diagnosis and treatment of nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is a malignant epithelial tumor originating in the nasopharynx and has a high incidence in Southeast Asia and North Africa.To develop these comprehensive guidelines for the diagnosis and management of NPC,the Chinese Society of Clinical Oncology(CSCO)arranged a multi-disciplinary team comprising of experts from all sub-specialties of NPC to write,discuss,and revise the guidelines.Based on the findings of evidencebased medicine in China and abroad,domestic experts have iteratively developed these guidelines to provide proper management of NPC.Overall,the guidelines describe the screening,clinical and pathological diagnosis,staging and risk assessment,therapies,and follow-up of NPC,which aim to improve the management of NPC. | Ling-Long Tang Yu-Pei Chen Chuan-Ben Chen Ming-Yuan Chen Nian-Yong Chen Xiao-Zhong Chen Xiao-Jing Du Wen-Feng Fang Mei Feng Jin Gao Fei Han Xia He Chao-Su Hu De-sheng Hu Guang-Yuan Hu Hao Jiang Wei Jiang Feng Jin Jin-Yi Lang Jin-Gao Li Shao-Jun Lin Xu Liu Qiu-Fang Liu Lin Ma Hai-Qiang Mai Ji-Yong Qin Liang-Fang Shen Ying Sun Pei-Guo Wang Ren-Sheng Wang Ruo-Zheng Wang Xiao-Shen Wang Ying Wang Hui Wu Yun-Fei Xia Shao-Wen Xiao Kun-Yu Yang Jun-Lin Yi Xiao-Dong Zhu Jun Ma | 2021 | Cancer Communications2021，41，11： | 22 |
| 11 | Resistin induces insulin resistance, but does not affect glucose output in rat-derived hepatocytes显示文摘目的: 现在的学习的目的是观察效果抵抗在在在导出老鼠的 hepatocytes.Methods 的胰岛素敏感度和葡萄糖输出上: 老鼠肝细胞瘤房间线 H4IIE 与有教养、刺激抵抗在; supernant 葡萄糖和肝糖内容被检测。胰岛素受体底层(红外)-1 和 IRS-2,蛋白质激酶 B/Akt,肝糖 synthase kinase-3 (3 (GSK-3p ) , suppress 或表明 3 的 cytokine (SOCS-3 ) ,蛋白质内容,以及磷酸化地位被西方的弄污估计。对 SOCS-3 指导的特定的反感觉 oligodeoxynucleotides 习惯于击倒的 SOCS-3.Results :抵抗素导致了抗胰岛素性,但是在老鼠肝细胞瘤房间的affect 葡萄糖输出没衬里 H4IIE.Resistin 胰岛素的稀释多重效果包括刺激胰岛素的肝糖合成和红外的磷酸化,蛋白质激酶 B/Akt ,象 GSK-3beta.Resistin 一样,处理显著地导致了基因和 SOCS-3 的蛋白质表示,胰岛素发信号的一个已知的禁止者。而且,对 SOCS-3 处理指导的特定的反感觉 oligodeoxynucleotide 阻止了抵抗在从反对胰岛素 action.Conclusion : 主要功能抵抗在在肝上是导致胰岛素 resistance.SOCS-3 感应的可以贡献胰岛素在 H4HE hepatocytes 发信号的调停 resistin 的抑制。 | Feng LIU Tao YANG Bin WANG Min ZHANG Nan GU Jie QIU Hong-qi FAN Chun-mei ZHANG Li FEI Xiao- qing PAN Mei GUO Rong-hua CHEN Xi-rong GUO | 2008 | Acta Pharmacologica Sinica2008，29，1： | 21 |
| 12 | Three-dimensional morphometric analysis for hepatectomy of centrally located hepatocellular carcinoma:A pilot study显示文摘AIM: To describe a three-dimensional model(3DM) to accurately reconstruct anatomic relationships of centrally located hepatocellular carcinomas(HCCs).METHODS: From March 2013 to July 2014, reconstructions and visual simulations of centrally located HCCs were performed in 39 patients using a 3D subject-based computed tomography(CT) model with customdeveloped software. CT images were used for the 3D reconstruction of Couinaud's pedicles and hepatic veins, and the calculation of corresponding tumor territories and hepatic segments was performed using Yorktal DMIT software. The respective volume, surgical margin, and simulated virtual resection of tumors were also estimated by this model preoperatively. All patients were treated surgically and the results were retrospectively assessed. Clinical characteristics, imaging data, procedure variables, pathologic features, and postoperative data were recorded and compared to determine the reliability of the model.RESULTS: 3D reconstruction allowed stereoscopic identification of the spatial relationships between physiologic and pathologic structures, and offered quantifiable liver resection proposals based on individualized liver anatomy. The predicted values were consistent with the actual values for tumor mass volume(82.4 ± 109.1 m L vs 84.1 ± 108.9 m L, P = 0.910), surgical margin(10.1 ± 6.2 mm vs 9.1 ± 5.9 mm, P = 0.488), and maximum tumor diameter(4.61 ± 2.16 cm vs 4.53 ± 2.14 cm, P = 0.871). In addition,the number and extent of portal venous ramifications, as well as their relation to hepatic veins, were visualized. Preoperative planning based on simulated resection facilitated complete resection of large tumors located in the confluence of major vessels. And most of the predicted data were correlated with intraoperative findings.CONCLUSION: This 3DM provides quantitative morphometry of tumor masses and a stereo-relationship with adjacent structures, thus providing a promising technique for the management of centrally located HCCs. | Fei Tian Jian-Xiong Wu Wei-Qi Rong Li-Ming Wang Fan Wu Wei-Bo Yu Song-Lin An Fa-Qiang Liu Li Feng Chao Bi Yun-He Liu | 2015 | World Journal of Gastroenterology2015，21，15： | 21 |
| 13 | Effect of curettage and copper wire on rabbit endometrium: a novel rabbit model of endometrial mechanical injury显示文摘它仍然是的背景几乎,为周期性的培植失败和怀孕损失的一种无助的状况目前由子宫内膜的损害引起了。这研究的目的是开发能为这困难的临床的 predicament.Methods 提供一个研究平台的子宫内膜的机械损害的一个兔子模型三个实验被进行。实验 1:在子宫角并且铜丝在一只角在刮术(双损害) 以后插入的刮术。组织学的变化在 0 点被监视, 24, 48, 72 个小时,以及在在操作以后的 1 和 2 个星期内。实验 2:指导在另外的角在一只角和双损害插入的铜丝。在两只角的电线在 2 个星期以后被移开。组织学的变化在电线移动以后在 0, 1 和 2 个星期被记录。实验 3:在一只角的双损害过程被执行,电线在 2 个星期以后被移开;另一只角被仍然是用作控制的正常。组织学的变化被记录,组织区域被测量,并且增长索引 PI, %) 在电线移动以后在 1,2, 4 和 8 个星期被计算,实验揭示了的 respectively.Results 由简单刮术或铜丝的受伤子宫内膜能是,这充分修理了。当子宫内膜的新生被双损害严重地损害时,子宫内膜和子宫的洞的两个区域减少了(P 0.05 ) ;两 PI 腺上皮并且在 4 个星期(P 0.05 ) 增加并且到达,但是由 8 weeks.Conclusion 归还了这研究最大值的 stromal 房间证明子宫内膜的损害的一个兔子模型能有效地与可比较的临床的索引通过 curettaqe 和铜丝的一个双损害过程被建立。 | LI Li SHIjing ZHANG Qiu-fang YAN Jie YAN Li-ying SHEN Fei QIAO Jie FENG Huai-liang | 2011 | Chinese Medical Journal2011，，11： | 20 |
| 14 | Fast magnetic reconstruction of the portal vein with allogeneic blood vessels in canines显示文摘BACKGROUND:The resection and reconstruction of large vessels,including the portal vein,are frequently needed in tumor resection.Warm ischemia before reconstruction might have deleterious effects on the function of some vital organs and therefore,how to reconstruct the vessels quickly after resection is extremely important.The present study was to introduce a new type of magnetic compression anastomosis(MCA)device to establish a quick non-suture anastomosis of the portal vein after resection in canines.METHODS:The new MCA device consists of a pair of titanium alloy and neodymium-ferrum-boron magnet(Ti-Nd Fe B)composite rings.The Nd Fe B magnetic ring as a core of the device was hermetically sealed inside the biomedical titanium alloy case.Twelve canines were divided into two groups:a MCA group in which the end-to-end anastomoses was made with a new device after resection in the portal vein and a traditional manual suture(TMS)group consisted of 6 canines.The anastomosis time,anastomotic patency and quality were investigated at week 24 postoperatively.RESULTS:The portal vein was reconstructed successfully in all of the animals and they all survived.The duration of portal vein anastomosis was significantly shorter in the MCA group than in the TMS group(8.16±1.25 vs 36.24±2.17 min,P<0.05).Portography and ultrasound showed that the blood flow was normal without angiostenosis or thrombosis in all of the canines.Hematoxylin-eosin staining and electron microscope scanning showed in contrast to the TMS group,MCA anastomotic intimal was much smoother with more regularly arranged endothelial cells at week 24 postoperatively. | Shan-Pei Wang Xiao-Peng Yan Fei Xue Ding-Hui Dong Xu-Feng Zhang Feng Ma Hao-Hua Wang Yi Lv | 2015 | Hepatobiliary & Pancreatic Diseases International2015，14，3： | 20 |
| 15 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 16 | Intestinal immune barrier integrity in rats with nonalcoholic hepatic steatosis and steatohepatitis显示文摘背景不含酒精的脂肪肝疾病( NAFLD )作为在这篇文章的 NAFLD.The 目的 pathogenis 的一个重要角色是完全在 NAFLD.Methods 的发展期间估计肠的有免疫力的障碍功能的长期的肝 injury.Intestinal 障碍戏的主要原因出现了 60 只男 Sprague-Dawley ( SD )老鼠被划分成 2 groups:normal 食谱( ND )组和高脂肪的饮食( HFD ) group.NAFLD 老鼠模型在 HFD 老鼠 group.Portal 血内毒素水平被建立被 li | Su Lin Wang Jian-hua Cong Xu Wang Li-hong Liu Feng Xie Xing-wang Zhang Heng-hui Wang Jiang-hua Fei Ran Liu Yu-lan | 2012 | Chinese Medical Journal2012，，2： | 18 |
| 17 | Macrophagic CD146 promotes foam cell formation and retention during atherosclerosis显示文摘 | Yongting Luo Hongxia Duan Yining Qian Liqun Feng Zhenzhen Wu Fei Wang Jing Feng Dongling Yang Zhihai Qin Xiyun Yan | 2017 | Cell Research2017，27，3： | 18 |
| 18 | Effect of icarisid II on diabetic rats with erectile dysfunction and its potential mechanism via assessment of AGEs, autophagy, roTOR and the NO-cGMP pathway显示文摘可勃起的机能障碍(编辑) 是糖尿病 mellitus 的主要复杂并发症。Icariin 被显示了通过它的 bioactive 形式提高可勃起的功能, icarisid II。这研究经由对先进 glycosylation 结束的评价与编辑和它的潜在的机制在糖尿病的老鼠上调查 icarisid II 的效果产品(年龄) , autophagy, mTOR 和 NO-cGMP 小径。Icarisid II 被一个酶的方法从 icariin 提取。在控制和糖尿病的编辑组,老鼠被管理正常盐;在 icarisid II 组,老鼠 intragastrically 是管理 icarisid II。可勃起的功能被测量 intracavernosal 压力 / 平均数评估动脉的压力(ICP/MAP ) 。年龄集中,氮的氧化物 synthase (NOS ) 活动和 cGMP 集中被酶免疫分析估计。房间增长被分析使用甲基 thiazolyl tetrazolium 试金和流动 cytometry。Autophagosomes 被传播电子显微镜学, monodansylcadaverine 染色和 GFP-LC3 本地化观察。在 autophagy 的 NOS isoforms 和关键蛋白质的表示被西方的污点检验。我们的结果证明了 Icarisid II 增加了 ICP/MAP 价值,光滑的肌肉房间(SMC ) 生长曲线, S 阶段和 SMC/collagen 纤丝(SMC/CF ) 比例和减少的 Beclin 1 (P< 0.05 ) 。Icarisid II 显著地增加了 proliferative 索引和 p-p70S6K (Thr389 ) 层次并且减少 autophagosomes 的数字和 LC3-II 的层次(P< 0.01 ) 。Icarisid II 减少了年龄集中和增加的 cGMP 集中, NOS 活动(P< 0.05 ) 并且 cNOS 层次(P< 0.01 ) 在糖尿病的编辑组。因此, Icarisid II 组成有希望的混合物因为糖尿病的编辑和力量涉及 SMC 增长和 NO-cGMP 小径和年龄, autophagy 和 mTOR 小径的 downregulation 的 upregulation。 | Jian Zhang Ai-Min Li Bao-Xing Liu Fei Han Feng Liu Shao-Peng Sun Xin Li Shu-Jin Cui Shao-Zhong Xian Guang-Qi Kong Zhong-Cheng Xin Zhi-Li Ji | 2013 | Asian Journal of Andrology2013，15，1： | 17 |
| 19 | Timing, distribution, and microbiology of infectious complications after necrotizing pancreatitis显示文摘BACKGROUND Acute pancreatitis(AP)is a common acute abdominal disease worldwide,and its incidence rate has increased annually.Approximately 20%of AP patients develop into necrotizing pancreatitis(NP),and 40%to 70%of NP patients have infectious complications,which usually indicate a worse prognosis.Infection is an important sign of complications in NP patients.AIM To investigate the difference in infection time,infection site,and infectious strain in NP patients with infectious complications.METHODS The clinical data of AP patients visiting the Department of General Surgery of Xuanwu Hospital of Capital Medical University from January 1,2014 to December 31,2018 were collected retrospectively.Enhanced computerized tomography or magnetic resonance imaging findings in patients with NP were included in the study.Statistical analysis of infectious bacteria,infection site,and infection time in NP patients with infectious complications was performed,because knowledge about pathogens and their antibiotic susceptibility patterns is essential for selecting an appropriate antibiotic.In addition,the factors that might influence the prognosis of patients were analyzed.RESULTS In this study,539 strains of pathogenic bacteria were isolated from 162 patients with NP infection,including 212 strains from pancreatic infections and 327 strains from extrapancreatic infections.Gram-negative bacteria were the main infectious species,the most common of which were Escherichia coli and Pseudomonas aeruginosa.The extrapancreatic infection time(9.1±8.8 d)was earlier than the pancreatic infection time(13.9±12.3 d).Among NP patients with early extrapancreatic infection(<14 d),bacteremia(25.12%)and respiratory tract infection(21.26%)were predominant.Among NP patients with late extrapancreatic infection(>14 d),bacteremia(15.94%),respiratory tract infection(7.74%),and urinary tract infection(7.71%)were predominant.Drug sensitivity analysis showed that P.aeruginosa was sensitive to enzymatic penicillins,thirdand fourth-generation cephalosporins,and carbapenems.Acinetobacter baumannii and Klebsiella pneumoniae were sensitive only to tigecycline;Staphylococcus epidermidis and Enterococcus faecium were highly sensitive to linezolid,tigecycline,and vancomycin.CONCLUSION In this study,we identified the timing,the common species,and site of infection in patients with NP. | Jiong-Di Lu Feng Cao Yi-Xuan Ding Yu-Duo Wu Yu-Lin Guo Fei Li | 2019 | World Journal of Gastroenterology2019，25，34： | 17 |
| 20 | Prolonged exposure to resistin inhibits glucose uptake in rat skeletal muscles显示文摘瞄准:在 basaland 上估计 resistin 的行动的效果和机制在老鼠的刺激胰岛素的葡萄糖举起骨胳的肌肉 cells.Methods:老鼠 myoblasts ( L6 ) werecultured 并且区分了进刺激与单个商业 resistin 跟随的 myotubes ( 130 ng/mL , 0-24 h )或从有 resistin-expressingvectors 的 293-T 房间 transfected 的有教养的上层清液( 130 ng/mL , 0-24 h ) .Liquid 焕发数被用来测定[ 3H ]胰岛素敏感的葡萄糖 transporters GLUT4 andGLUT1 的 2-deoxyglucoseuptake .The translocation , 联系synaptosomal 的蛋白质 23 ( SNAP23 )并且充斥蛋白质内容,以及 tyrosinephosphorylation statu 有单个 resistin 或有教养的 supernatantcontaining recombinant resistin 的 L6 myotubes 的处理减少了基础、刺激胰岛素的 2-deoxyglucose 举起 andimpaired 刺激胰岛素的 GLUT4 translocation.While SNAP23 蛋白质内容被减少, noeffects 在 GLUT4 或没有影响它的蛋白质内容,也减少的 insulin-stimulatedIRS-1 酷氨酸 phosphorylation 铺平的 GLUT1 蛋白质内容 Resistin 被注意从有 resistin-expr 的 293-T 房间 transfected 的效果 ofrecombinant resistin Resistin 调整了 IRS-1 功能并且减少响应 SNAP23 mayhave 的 insulin.The downregulated 表示的 GLUT4translocation 和葡萄糖举起部分由 resistin 处理 Theseobservations 热点归因于葡萄糖举起的减少在到肥胖的类型 2 diabetesrelated 的 pathophysiology 的 resistin 的潜在的角色。 | Hong-qi FAN Nan GU Feng LIU Li FEI Xiao-qin PAN Mei GUO Rong-hua CHEN Xi-rong GUO | 2007 | Acta Pharmacologica Sinica2007，28，3： | 16 |
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