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| 1 | ZFN,TALEN and CRISPR-Cas9 mediated homology directed gene insertion in Arabidopsis:A disconnect between somatic and germinal cells显示文摘Breakthroughs in the generation of programmable sequencespecific nucleases (SSNs),such as zinc finger nucleases (ZFNs), TAL effector nucleases (TALENs)and the RNA-directed nuclease CRISPR-associated protein 9 (Cas9),have greatly increased the ease of plant genome engineering (Voytas,2013;Malzahn et al., 2017).Programmable SSNs introduce a DNA double-strand break (DSB)at a target site in the genome that must be repaired by one of several endogenous DNA repair pathways. | Qiwei Shan Nicholas J.Baltes Paul Atkins Elida R. Kirkland Yong Zhang Joshua A.Ballet Levi G.Lowder Aimee A.Malzahn John C.Haugner Ⅲ Burckhard Seelig Daniel F. Voytas Yiping Qi | 2018 | Journal of Genetics and Genomics2018，45，12： | 9 |
| 2 | Trophic factors are essential for the survival of grafted oligodendrocyte progenitors and for neuroprotection after perinatal excitotoxicity显示文摘The consequences of neonatal white matter injury are devastating and represent a major societal problem as currently there is no cure.Prematurity,low weight birth and maternal pre-natal infection are the most frequent causes of acquired myelin deficiency in the human neonate leading to cerebral palsy and cognitive impairment.In the developing brain,oligodendrocyte(OL)maturation occurs perinatally,and immature OLs are particularly vulnerable.Cell replacement therapy is often considered a viable option to replace progenitors that die due to glutamate excitotoxicity.We previously reported directed specification and mobilization of endogenous committed and uncommitted neural progenitors by the combination of transferrin and insulin growth factor 1(TSC1).Here,considering cell replacement and integration as therapeutic goals,we examined if OL progenitors(OLPs)grafted into the brain parenchyma of mice that were subjected to an excitotoxic insult could rescue white matter injury.For that purpose,we used a well-established model of glutamate excitotoxic injury.Four-day-old mice received a single intraparenchymal injection of the glutamate receptor agonist N-methyl-D-aspartate alone or in conjunction with TSC1 in the presence or absence of OLPs grafted into the brain parenchyma.Energetics and expression of stress proteins and OL developmental specific markers were examined.A comparison of the proteomic profile per treatment was also ascertained.We found that OLPs did not survive in the excitotoxic environment when grafted alone.In contrast,when combined with TSC1,survival and integration of grafted OLPs was observed.Further,energy metabolism in OLPs was significantly increased by N-methyl-D-aspartate and modulated by TSC1.The proteomic profile after the various treatments showed elevated ubiquitination and stress/heat shock protein 90 in response to N-methyl-D-aspartate.These changes were reversed in the presence of TSC1 and ubiquitination was decreased.The results obtained in this pre-clinical study indicate that the use of a combinatorial intervention including both trophic support and healthy OLPs constitutes a promising approach for long-term survival and successful graft integration.We established optimal conditioning of the host brain environment to promote long-term survival and integration of grafted OLPs into an inflamed neonate host brain.Experimental procedures were performed under the United States Public Health Service Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care Committee at(UCLA)(ARC#1992-034-61)on July 1,2010. | Megumi Hirose-Ikeda Brian Chu Paul Zhao Omar Akil Elida Escalante Laurent Vergnes Carlos Cepeda Araceli Espinosa-Jeffrey | 2020 | Neural Regeneration Research2020，15，3： | 4 |
| 3 | Toll-like receptor 4 polymorphisms and bacterial infections in patients with cirrhosis and ascites显示文摘AIM To assess the relationship between the presence of toll-like receptor 4(TLR4) polymorphisms and bacterial infections in cirrhotic patients with ascites. METHODS We prospectively included consecutive patients with cirrhosis and ascites hospitalized during a 6-year period. Patients with human immunodeficiency virus(HIV) infection or any other immunodeficiency, patients with advanced hepatocellular carcinoma(beyond Milan's criteria) or any other condition determining poor short-term prognosis, and patients with a permanent urinary catheter were excluded. The presence of D299 G and/or T399 I TLR4 polymorphisms was determined by sequencing and related to the incidence and probability of bacterial infections, other complications of cirrhosis, hepatocellular carcinoma, and mortality during follow-up. A multivariate analysis to identify predictive variables of mortality in the whole series was performed. RESULTS We included 258 patients: 28(10.8%) were carriers of D299G and/or T399I TLR4 polymorphisms(polymorphism group) and 230 patients were not(wildtype group). The probability of developing any bacterial infection at one-year follow-up was 78% in the polymorphism group and 69% in the wild-type group(P = 0.54). The one-year probability of presenting infections caused by gram-negative bacilli(51% vs 44%, P = 0.68), infections caused by gram-positive cocci(49% vs 40%, P = 0.53), and spontaneous bacterial peritonitis(29% vs 34%, respectively, P = 0.99) did not differ between the two groups. The oneyear probability of transplant-free survival was 55% in the polymorphism group and 66% in the wild-type group(P = 0.15). Multivariate analysis confirmed that age, Child-Pugh score, active alcohol intake, previous hepatic encephalopathy, hepatocellular carcinoma and serum creatinine were associated with a higher risk of death during follow-up. CONCLUSION Genetic polymorphisms D299 G and/or T399 I of TLR4 do not seem to play a relevant role in the predisposition of cirrhotic patients with ascites to bacterial infections. | Edilmar Alvarado-Tapias Carlos Guarner-Argente Elida Oblitas Elisabet Sánchez Silvia Vidal Eva Román Mar Concepción Maria Poca Cristina Gely Oana Pavel Juan Camilo Nieto Cándido Juárez Carlos Guarner Germán Soriano | 2018 | World Journal of Hepatology2018，10，1： | 3 |
| 4 | 2014年猪场五种病毒性疾病的血清抗体检测显示文摘采用ELISA方法,对186个中小规模猪场的母猪群、保育猪群和育肥猪群的1400余份血清检测猪瘟、伪狂犬病、猪繁殖与呼吸障碍综合征、圆环病毒2型和口蹄疫O型的抗体水平。结果表明,75%~87%的猪群抗体水平合格,种猪群抗体合格率高于保育猪群和育肥猪群。伪狂犬病野毒gE抗体阳性率较高,母猪群中gE抗体阳性样品数占检测样品总数的40.93%,而母猪群中阳性场占总场数的89.74%。 | Gloria Solano Elida Bautista Thomas Molitor Joaquim Segalés Carlos Pijoan 丁文格 朱秀高 叶晶 侯博 蒋文明 石勇耀 程福生 樊学坤 陆军 熊剑锋 唐万勇 | 2015 | 中国猪业2015，10，8： | 2 |
| 5 | Cloning of the chaperonin t-complex polypeptide 1 gene from Schistosoma mansoni and studies of its expression levels under heat shock and oxidative stress 显示文摘 | Elida G C Fadi F H | 2000 | Parasitology Research2000，86，： | 1 |
| 6 | Painful ophthalmoplegia with reversible carotid stenosis in a child 显示文摘 | Mireia DT Alfons MC Elida VZ | 2001 | Pediatr Neurol2001，24，4： | 1 |
| 7 | Long-term decrease in immediate early gene expression after electroconvulsive seizures显示文摘 | Julien Braga Calais Samira S. Valvassori Wilson R. Resende Gustavo Feier Maria Carolina Pedro Athié Sidarta Ribeiro Wagner Farid Gattaz Jo?o Quevedo Elida Benquique Ojopi | 2013 | Journal of Neural Transmission2013，，2： | 1 |
| 8 | Hydro-geochemistry of groundwater and surface water in Dschang town(West Cameroon):Alkali and alkaline-earth elements ascertain lithological and anthropogenic constraints显示文摘The study aims to identify a suitable site for open and bore well in a farmhouseusing ground magnetic survey in south India.It also aims to define depth to granitoid and structural elements which traverse the selected area.Magnetic data(n=84)measured,processed and interpreted as qualitative and quantitatively.The results of total magnetic intensities indicate that the area is composed of linear magnetic lows trending NE-SW direction and circular to semi-circular causative bodies.The magnetic values ranged from-137 nT to 2345 nT with a mean of 465 nT.Reduction to equator shows significant shifting of causative bodies in the southern and northern directions.Analytical signal map shows exact boundary of granitic bodies.Cosine directional filter has brought out structural element trending NE-SW direction.Results of individual profile brought to light structurally weak zone between 90 m and 100 m in all the profile lines.Sudden decrease of magnetic values from 2042 nT to 126 nT noticed in profile line 6 between 20 m and 30 m indicates fault occurrence.Magnetic breaks obtained from these maps were visualized,interpreted and identified two suitable sites for open and bore well.Radially averaged power spectrum estimates depth of shallow and deep sources in 5 m and 50 m,respectively.Euler method has also been applied to estimate depth of granitoid and structural elements using structural indexes 0,1,2,and 3 and found depth ranges from<10 m to>90 m.Study indicates magnetic method is one of the geophysical methods suitable for groundwater exploration and site selection for open and borewells. | Yemeli Elida Joelle Temgoua Emile Kengni Lucas Ambrosi Jean-Paul Momo-nouazi Mathieu Silatsa-Tedou Francis Brice Wamba Franck Robean Tchakam-Kamtchueng Brice | 2021 | Journal of Groundwater Science and Engineering2021，9，3： | 1 |
| 9 | A chemilumi nescent flow sensing device for determination of choline and phos pbolipase d activity in biological samples显示文摘 | Pavel Rauch Elida N Ferri Stefano Girotti | 1997 | Analytical Biochem istry1997，，245： | 1 |
| 10 | Monitoring of environ-mental pollutants by bioluminescent bacteria显示文摘 | Stefano G Elida N F Maria G F | 2008 | Analytica Chimica Acta2008，608，1： | 1 |
| 11 | Quantitation of porcine reproductive and respiratory syndrome virus RNA in semen by single-tube reverse transcription nested polymerase chain reaction 显示文摘 | JINHO S ELIDA M B YUNG -BAI KANG | 1998 | Journal of Virological Methods1998，72，： | 1 |
| 12 | Mechanical properties and stem cell adhesion of injection‐molded poly(ether ether ketone) and hydroxyapatite nanocomposites显示文摘 | Bruna Turino Rego Wilson Alves Ribeiro Neto Ana Claudia Chagas de Paula Alfredo Miranda Góes Rosario Elida Suman Bretas | 2015 | J. Appl. Polym. Sci2015，，14： | 1 |
| 13 | Cloning of the chaperonin t -complex polypeptide 1 gene from Schistosoma mansoni and studies of its expression levels 显示文摘 | ELIDA G FADI C HAMDAN F | 2000 | Parasitol Res2000，86，： | 1 |
| 14 | Internal friction and loss tangent of nonlinear viscoelastic materials:different concepts,different results显示文摘 | Elida B H Povolo F Porta P | 2000 | Journal of Alloys and Compounds2000，310，： | 1 |
| 15 | Effects of fructooligosaccharides on intestinal flora and human health显示文摘 | Hidaka H Elida T Takizawa T | 1986 | Bifidobacteria Microflora1986，5，： | 1 |
| 16 | Is 27 a Big Number? Correlational and Causal Connections Among Numerical Categorization, Number Line Estimation, and Numerical Magnitude Comparison显示文摘 | Elida V.Laski Robert S.Siegler | 2007 | Child Development2007，，6： | 1 |
| 17 | Equine Botulinum Antitoxin for the Treatment of Infant Botulism显示文摘 | Elida E Vanella de Cuetos Rafael A | 2011 | clinical and vaccine immunology2011，18，11： | 1 |
| 18 | Monitoring of environmental pollutants by bioluminescent bacteria 显示文摘 | Stefano Girotti Elida Nora Ferri Maria Grazia Fumo Elisabetta Maiolini | 2005 | Analytica chimica acta2005，608，： | 1 |
| 19 | Endoplasmic reticulum stress:signaling the unfolded protein response 显示文摘 | Elida L Traey T Allen V | 2007 | Physiology2007，22，3： | 1 |
| 20 | PI3K/Akt inhibition modulates multidrug resistance and activates NF-κB in murine lymphoma cell lines显示文摘 | Mariana G. García Laura D. Alaniz Rosalía I. Cordo Russo Elida Alvarez Silvia E. Hajos | 2008 | Leukemia Research2008，，2： | 1 |
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